• Korean Journal of Food Science and Technology
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• v.20 no.3
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• pp.433-440
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• 1988

Dried anchovy (Engraulis japonica) was ground and treated with 0.3N NaOH solution and then hydrolyzed with proteolytic enzymes. Extracts obtained by centrifugation of alkali-enzyme treated anchovy slurry was compared with water extract for the yields of soluble solid, protein and ashes and organoleptic characteristics. The data for the yields of the soluble solids, protein and ash showed that a 2-3 folds increase in those yields was resulted by combined alkali-enzyme treatments when it was compared to water only extract. The organoleptic evaluation on the alkali-enzyme treated anchovy extracts also showed a 2-3 folds in flavor strength of all descriptions in odor and taste and a significant improvement in total odor or taste acceptability.

• Applied Biological Chemistry
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• v.40 no.1
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• pp.43-47
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• 1997

The weight ratio of the seed in jujube averaged to 19.1%. The chemical components of dried pitted-jujube were moisture 20.4%, protein 5.3%, fat 1.3%, ash 2.7%, fiber 4.4% and nitrogen-free extract 65.9%. Alcohol insoluble solid content of dried pitted-jujube was 17.4%, in which ammonium oxalate-soluble pectin was 3.7%, water-soluble pectin 3.0%, hydrochloric acid-soluble pectin 1.0%, and sodium hydroxide-soluble pectin 1.0%. Jujube contained sucrose, glucose and fructose as free sugar. The sugars of dried pitted-jujube was composed of sucrose 48.1%, fructose 18.7%, and glucose 11.3%. Drying experiments of pitted-jujube and unpitted-jujube were carried out. Moisture content and brix of dried pitted-jujube were reached at that of dried unpitted-jujube(11%, 78 brix in 48 hours of dry) in 11 hours of drying. And this means that dried pitted-jujube dried 4 times faster than dried unpitted-jujube. The extracts of dried pitted-jujube and dried unpitted-jujube were examinet according to the amount of water added and the extraction time. The brix of dried pitted-jujube extract for 30 min is similar to that of dried unpitted-jujube extract for 150 min. The brix of the extracts did not increase after 150 min extraction. As to the color of extract, 'L' value was higher in dried unpitted-be, while 'a' and 'b' values were higher in dried pitted-jujube.

• Applied Microscopy
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• v.22 no.1
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• pp.70-78
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• 1992

Antibacterial effects of the water-soluble extract of Ulmus pumila L. on Staphylococcus aureus and Escherichia coli were studied by cylinder plate dilution method. The cells of S. aureus and E. coli treated with the extract were comparatively examined by scanning and transmission electron microscopies for their morphological features. The normal cells of S. aureus and E. coli showed thypical coccus and rod shapes, respectively, but the cells of S. aureus treated with the extract showed rough surface structures with many granular protrusions and were destroyed to form ghost cells by liberating their cytoplasmic components. E. coli cells treated with the extract were destoryed without enlarging in size and producing granules on their surfaces.

• Journal of Applied Biological Chemistry
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• v.65 no.4
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• pp.337-341
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• 2022

In this study, the effect of the combined use of a lipid-soluble n-hexane extract of red ginseng marc (HERGM) and water-soluble arginine, which exhibits anticaries activity, on the growth of Streptococccus mutans was investigated. As a result of checkerboard assay, HERGM and arginine showed a synergistic effect in inhibiting the growth of S. mutans with a fractional inhibitory concentration index of 0.396. Combination treatments of HERGM and arginine resulted in leakage of nucleic acid components and decrease in the viable cell counts of S. mutans, all of which were proportional to the concentration of HERGM. In conclusion, the synergistic effect of HERGM and arginine on the growth inhibition of S. mutans is mainly attributed to HERGM.

• Journal of the Korean Society of Tobacco Science
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• v.10 no.2
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• pp.99-107
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• 1988

Pot experiment was conducted to find out the effect of lime application on yield and chemical composition of burley tobacco in 1986, Lime increased exchangeable calcium and pH of soil, but decreased Al, Fe and Mn concentrations. Yield was increased by lime application, however lime could not be caused to yield increasing in the soil with high calcium contents. Cored leaves of limed Plot contained higher Mg. K, total nitrogen, NO3-nitrogen, water soluble and insoluble ash, alkalinity number of water soluble and insoluble ash, citric and malic acid, but lower Fe, Mn, protein-nitrogen, NH3-nitrogen, nicotine petroleum ether extract, palmitic and linolenic acid concentrations than those of unlined plot. The linoleic acid and volatile neutral constituents of cured leaves were not affected by liming. Lime increased yield, however it did not affect leaf duality in respect to chemical characteristics, suggesting that liming should be necessary for tobacco cultivation.

• Korean journal of food and cookery science
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• v.22 no.6 s.96
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• pp.905-913
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• 2006

The properties of water soluble powder of propolis(WSP), made with different levels(0, 20, 40, 60, 80%) of ethanol extract of propolis(EEP) and hydrocolloid were investigated, along with the quality changes of its bread after 7 days' of storage at $30^{\circ}C$ The yield of WSP containing 40% EEP treated at $160^{\circ}C$ was the highest at 59.3% and the brown color of all the powders tended to be darkened with increasing EEP content. The turbidity of WSP treated at higher temperature was decreased in its aqueous solution (10%, w/w), and this was considered to be due to the presence of minute nonsoluble particles. Antioxidative activities determined by DPPH(1,1-diphenyl-2-picrylhydrazyl) were the lowest in WSP treated at $140^{\circ}C$, while those of the WSP samples prepared at 160 and $180^{\circ}C$ were as high as that of WSP containing more than 40% EEP, regardless of EEP concentration. The propolis breads with added WSP made at $160^{\circ}C$ were selected as the most desirable powder for subsequent study. Bread with WSP40 was the heaviest while the volume loss of WSP80 was the greast after baking. The moisture contents of the propolis bread were drastically decreased until 3 days' of storage, but it was thought that WSP might be ineffective for the prevention of moisture loss. The pH of breads without EEP was decreased after 3 days' of storage, while that of the WSP breads remained almost unchanged until 5 days' of storage. Total bacterial counts also exhibited decay levels during the storage. In conclusion, water soluble powder of propolis is useful as a natural antioxidative and antibacterial material in various types of food.

• Korean Journal of Food Science and Technology
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• v.33 no.2
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• pp.161-165
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• 2001

The dietary fiber was extracted from apple pomace with various concentrations of acidic, alkaline solvents and water in $24.4{\sim}29.3%$ yield. The extractions of water-soluble dietary fiber with acidic(6.85%) and alkaline solvent(6.55%) were more effective than the water extraction(6.0%). The higher extraction yield was obtained with increased concentration of acidic or alkaline solvents. The content of galacturonic acid was higher in the order, 0.01 M HCl(77.5%)>0.05 M HCl(76.8%)>$H_2O(76.1%)$>0.05 M NaOH(73.8%)> 0.01 M NaOH(69.0%) and the extraction with acidic solvent showed the highest pectin yield. The color value increased by extraction with solvent in higher concentration. And at equal concentration, the color value of alkaline extract was higher than that of acidic exctract. There was no relationship between the color value and the extraction yield of dietary fiber. In the investigation on the rheological properties, the shear stress increased in proportion to galacturonic acid contents. While water-soluble dietary fiber extracted with acid and water represented Newtonian rheological properties, the alkaline extract was inclined to have converting property from pseudoplastic to Bingham pseudoplastic with increment of alkaline concentration. In the studies on the time-dependence of apparent viscosity, the thixotropic property with hysteresis loop was observed in alkaline extract.

• YAKHAK HOEJI
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• v.28 no.4
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• pp.231-235
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• 1984

Active principles for the anti-fatigue activity of Panax ginseng were studied in mice using the swimming performance method. Ginseng water extract maximized the prolongation of swimming time 18 hours after administration. The potencies of anti-fatigue activities were found as in the order of ether soluble fraction and butanol soluble fraction as those of antioxidant activities previously determined. The anti-oxidant components, maltol, salicylic acid and vanillic acid isolated from the ether soluble fraction of Panax ginseng strongly exhibited the antifatigue activities, where as highly purified crystalline ginsenoside $4Rb_1$, Re and $4Rg_1$ did not.

• Journal of Food Hygiene and Safety
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• v.35 no.4
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• pp.382-390
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• 2020

This study was carried out to evaluate the antimicrobial effect of red ginseng (Panax ginseng C.A. Meyer) against several foodborne pathogens including Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus niger. The antimicrobial effect was determined by agar diffusion method using red ginseng extract, crude saponin and non-water-soluble fractions. Red ginseng extract showed antimicrobial effect against S. aureus, but not C. albicans or A. niger. The extract showed anti-bacterial activity at concentration above 30% against S. aureus, which cause both food poisoning and atophic dermatitis. Crude saponin showed antibacterial activity above 7.5% against the bacterium. However, the ginsenosides purified from crude saponin showed no antimicrobial activities at 100-200 ㎍/mL. To investigate the mode of growth inhibition, red ginseng extract and crude saponin were added to 0.85% NaCl solution containing S. aureus and then incubated at 35℃ for 12 h. The results showed that viable cells were rapidly reduced in above 10% concentration of red ginseng extract and above 2% of crude saponin, respectively. However, the crude saponin and red ginseng extract did not inhibit the bacterial cells completely at those same concentrations. On the other hand, whereas all non-water-soluble fractions showed inhibition zones above 10 mm against S. aureus, they showed no inhibition effects against E. coli, C. albicans or A. niger. The methanol fraction-1 (MF-1) showed the highest antibacterial activity against S. aureus, and the MIC (minimal inhibitory concentration) was 0.625 mg/mL. These results suggest that red ginseng extract, crude saponin and non-water-soluble fractions show selective antibacterial activity against S. aureus, and non-water-soluble fractions might be used as natural antibacterial agents.

• Journal of Pharmaceutical Investigation
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• v.8 no.1
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• pp.6-16
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• 1978

We obtained 4 kinds of extract fraction from Acanthopanacis Radicis Cortex and studied on the influence to the blood pressure of rabbit. These 4 fractions were obtained as follows; Fraction I was insoluble fraction by 99% ethanol from 80% methanol extract of Acanthopanacis Radicis Cortex, fraction II, precipitated fraction by ether from 99% ethanol soluble fraction of 80% methanol extract of Acanthopanaacis Radicis Cortex, fraction III, no precipitated fraction by ether from 99% ethanol soluble fraction of above 80% methanol extract and fraction IV, water extract of Acanthopanacis Radicis Cortex. All of fractions, when administered into ear-vein of rabbit, produced fall of blood pressure. Among these 4 fractions, although fraction III was not only the most potent but had the greatest efficacy, we observed the mechanism of hypotensive action of Acanthopanacis Radicis Cortex, making use of fraction II which was thought as a comparatively pure fraction. Hypotensive action of fraction II (APF II) was not affected by vagotominization but markedly inhibited by atropine. Pretreatment of bethanidine showed a tendency to weaken the depressor action of APF II, although it was not a significant result, but diphenhydramine did not influence APF II action. Phentolamine, guanethidine and chlorisondamine inhibited significantly the hypotensive action of APF II. APF II elicited the potentiation of norepinphrine pressor action dependent on the time-factor whereas it did not influence angiotesin pressor action. It is seemed that APF II exhibited hypotensive action, causing peripheral muscarinic-effect and centrally induced sympatholytic action.