• Korean Journal of Microbiology
• /
• v.9 no.4
• /
• pp.163-168
• /
• 1971

Neutral protease which was obtained from a genus of Aspergilli as the crystal form were investigated for their purification and properties. The results of biochemical and enzymatic studies for their purification and properties in this enzyme were as follows. 1) On the wheat media containing 70%-water and $CaCo_{3}$, Aspergilus oryzae S.H.W. 131 is satisfactorily grown under the basic optimum conditions temperature $27^{\circ}C$- $30^{\circ}C$at relative humidity 100% for three days. 2) The enzyme solution extracted with water is successively purified through the passing on column of Asmti-177N for decolorization of it. And ion exchanger such as DEAAE Sphadex A-50 or Shepadex G-100 and fraction collector is necessary for the sepearte treatments of this enzyme. After washing it with organic solvents as aceton-EtOH, etc., it should be dried on the vacuum dryer at $40^{\circ}C$) The protease activity is determined by the amounts of amino acids, tyrosine. 4) The optimum pH of neutral protease is 6.0-8.0. 5) In effectively decomposing with this neutral protease, the optimum temperature is $35^{\circ}C$. 6) It is interesting that the amounts of metal ion affects the activity of neutral protease. For examples, if it were treated with manganic ion, its activity would be more effective than any other that.

• Journal of Microbiology and Biotechnology
• /
• v.9 no.1
• /
• pp.56-61
• /
• 1999

Pseudomonas sp. SW1 grew and produced biosurfactants on 3% hexadecane as the energy and carbon source. As a result of biosurfactant synthesis, the surface tension of the medium was reduced from 72 dyne/cm to 30 dyne/cm. The properties of biosurfactants that were purified from Pseudomonas sp. SW1 were investigated. The purification procedure included acid precipitation from culture supernatant, silica gel G60 column chromatography, and Sephadex G-150 gel filtration. The biosurfactants were separated into two different types, viz., types I and II. Biosurfactant type Isignificantly reduced the surface tension of water from 72 to 27 dyne/cm at concentration levels above 30 mg/l. The surface tension of water was reduced to a minimum of approximately 30 dyne/cm by biosurfactant type II at concentration levels over 80 mg/l. The biosurfactants were effective in a wide range of pHs, at NaCl concentrations of up to 4%, at $CaCl_2$ concentration up to 100 mM, and at temperatures up to $200^{\circ}C$ for 8 h.

• Applied Biological Chemistry
• /
• v.24 no.2
• /
• pp.132-138
• /
• 1981

Four species of the photosynthetic bacteria from 36 samples were isolated. their characteristics, their capability for nitrogen fixation and their capability for purification of organic waste water by photosynthetic bacteria were examined. Photosynthetic bacteria are widely distributed in soil. The isolated species are Rhodopseudomonas capsulatus. R. spheroides, R. gelatinosa, and R. ruburm. The capability for nitrogen fixation varies with the species of photosynthetic bacteria, and it is very pronounced in the R. capsulatus. The capability for purification of organic waste water is relatively strong but varies with the species.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2010.06a
• /
• pp.44-44
• /
• 2010

Conventional water treatment facilities like precipitation process need large-scale equipment and wide space to purify the wastewater of paper factory. In case of massive waste water, high gradient magnetic separation (HGMS) parts are more effective to purify it rapidly and to occupy relatively small space, since large voids at filter with HGMS are adopted. Cryo-cooled Nb-Ti superconducting magnet with room temperature bore in diameter of 100 mm and 600 mm in height was used for magnetic separator. Magnetic filters were designed by the analysis of magnetic field distribution at superconducting magnets.

• The Korean Journal of Ecology
• /
• v.23 no.2
• /
• pp.163-167
• /
• 2000

To evaluate the water purification capacity of 4 emergent macrophytes in 4 tributaries of Mankyung River, nitrate reductase activity (NRA) and nutrient removal capacity were determined. Higher NRA occurred in emergent macrophytes such as Persicaria thunbergii and Oenanthe iavanica with 7.8 and 5.4 ${\mu}$moi NO$_2$ g$^{-1}$d.wt. h$^{-1}$. respectively. The nitrogen removal capacity of emergent macrophytes displaying higher NRA fell within the range of 0.85 to 1.95 mg g$^{-1}$d.wt. day$^{-1}$ and was higher in the order Phragmites communis > Persicaria thunbergii > Oenanthe iavanica > Zizania latifolia. The phosphorus removal capacity was within the range of 0.07 to 0.12 mg g$^{-1}$d.wt. day$^{-1}$ and was higher in the order Phragmites communis > Oenanthe iavanica > Persicaria thunbergii > Zizania latifolia. In all the domestic, industrial and agricultural wastewaters, Phragmites communis showed the highest nitrogen and phosphorus removal capacity; 1.36 and 0.0088 mg g$^{-1}$d.wt. day$^{-1}$ respectively. Among the 4 macrophytes. Phragmites communis was the most suitable species for water purification in 4 tributaries of Mankyung River.

• Journal of the Korean Wood Science and Technology
• /
• v.24 no.2
• /
• pp.20-25
• /
• 1996

Wood chips of oak(Quercus mongolica) and larch(Larix leptolepis) were exploded with the optimum condition. Main components of exploded wood were separated with hot hot water and methanol. The hemicelluloses were purified from hot water extracts and alditol complexs were prepared from purified hemicellulose. And also, cellulose nitrate was prepared from extractive residue and characterized. The results can be summarized as follows. 1. Amounts of carbohydrate(72~79%) in the crude hemicellulose of larch wood was more than those of oak wood(55~66%). 2. The crude hemicelluloses were mainly composed of oligosaccharides in oak wood but those in larch wood contained about 50% monosaccharides. 3. Decolorization of hemicellulose was successful with activated charcoal and ion-exchange resin treatment. The alditol yields were 56.3~82.9%. 4. The degree of substitution(D.S.) of cellulose nitrate was 1.95~2.87 and it showed a good acetone solubility.

• 유체기계공업학회:학술대회논문집
• /
• 2003.12a
• /
• pp.421-426
• /
• 2003

Recently, mixers are being widely used in the water purification plant in order to increase the filtration efficiency. The mixer normally consists of impeller, shaft, hub, reduction gear, and the driving motor. It is one of the key design issues to confirm that the vibration caused by the rotation of the shaft should not coincide with the natural vibration frequency of the shaft itself. The vibration characteristics of the hydrofoil type mixer, which is the most widely used in real plants are evaluated through the finite element modeling and verified by experiment using the mock-up facility. The fundamental natural frequency of the mixer's shaft is found to be around 1.8 Hz which showed in good agreement with the experiment. The higher natural frequencies to the 2nd, 3rd, and 4th modes are also verified by the experiment. Thus the developed model is to be utilized for the structural design of the real mixer system.

• Nuclear Engineering and Technology
• /
• v.49 no.3
• /
• pp.556-561
• /
• 2017

Serving as an excellent adsorbent and inorganic ion exchanger in the water purification field, zeolite 4A has in this work presented a strong capability for purifying radioactive waste, such as $Sr^{2+}$, $Cs^+$, and $Co^{2+}$ in water. During the processes of decontamination and decommissioning of suspended solids and organics in low-level radioactive wastewater, the purification performance of zeolite 4A has been studied. Under ambient temperature and neutral condition, zeolite 4A absorbed simulated radionuclides such as $Sr^{2+}$, $Cs^+$, and $Co^{2+}$ with an absorption rate of almost 90%. Additionally, in alkaline condition, the adsorption percentage even approached 98.7%. After conducting research on suspended solids and organics of zeolite 4A for the treatment of radionuclides, it was found that the suspended clay was conducive to absorption, whereas the absorption of organics in solution was determined by the species of radionuclides and organics. Therefore, zeolite 4A has considerable potential in the treatment of radioactive wastewater.

• Journal of environmental and Sanitary engineering
• /
• v.9 no.1
• /
• pp.1-16
• /
• 1994

Water environment is vital for our daily life, especially all living things, next to the air environment we breathe. In this regard, Great Britain is known as one of the most developed and well experienced countries which has developed a comprehensive system of the water environment management. In England and Wales, the National Rivers Authority was established in 1989, as an independent regulatory body taking over from the 10 Regional Water Authorities responsibility for the management of water environment. In Scotland, responsibility rests River Purification Boards and Island Council while, the Department of the Environment is the sole water authority for Northern Ireland. Overall results has made by the authority together with their strategies are reviewed in this paper.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2005.05b
• /
• pp.1254-1258
• /
• 2005

Net outflow flux and unit load of pollutants were investigated at a paddy fields area(Soro-ri) with large-scaled plots on loam soil during irrigation seasons of $2001\~2003$. Water samples were collected, and inflow and outflow were .measured at $5\~10$ day intervals during non-storm periods and at $2\~6$ hours intervals during storm events. The average concentration of TP in percolated water was much smaller than that in irrigation and surface outflow water likely due to phosphorus absorption capacity of paddy soils. Net outflow flux in study area was significantly correlated with rainfall and surface outflow discharge. Nutrient flux from Paddy fields can be abated by reduction in outflow surface discharge.