• Environmental Engineering Research
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• 제24권3호
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• pp.397-403
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• 2019

Microcystis sp. is one of the most common harmful cyanobacteria that release toxic substances. Counting algal cells is often used for effective control of harmful algal blooms. However, Microcystis sp. is commonly observed as a colony, so counting individual cells is challenging, as it requires significant time and labor. It is urgent to develop an accurate, simple, and rapid method for counting algal cells for regulatory purposes, estimating the status of blooms, and practicing proper management of water resources. The flow cytometer and microscope (FlowCAM), which is a dynamic imaging particle analyzer, can provide a promising alternative for rapid and simple cell counting. However, there is no accurate method for counting individual cells within a Microcystis colony. Furthermore, cell counting based on two-dimensional images may yield inaccurate results and underestimate the number of algal cells in a colony. In this study, a three-dimensional cell counting approach using a novel model algorithm was developed for counting individual cells in a Microcystis colony using a FlowCAM. The developed model algorithm showed satisfactory performance for Microcystis sp. cell counting in water samples collected from two rivers, and can be used for algal management in fresh water systems.

• 한국수산과학회지
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• 제31권1호
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• pp.109-113
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• 1998

황토에 의한 Cochlodinium 종의 제거효과실험을 실내와 현장조사를 통하여 실시하였다. 실내실험은 Cochiodinium 적조생물을 배양하여 황토 및 이를 산처리로 활성화시켜 살포농도를 $2\;g/\ell$에서 $10\lg/\ell$로하여 제거효율을 조사하였다. 황토의 적조생물제 거효과는 살포농도 $2\;g/\ell$에서 $43\%$, $6\;g/\iota$에서 $64\%$로 점차 향상되기 시작하여 $10g/{\ell}$에서는 살포 60분후 $88\%$의 제거효과를 나타냈다. 현장조사는 Cochlodinium 적조가 빈발한 통영 활목-오비도해역에서 1996년 9월에 2회 실시되었다. 황토살포 전후로 해서 실시된 조사에서도 역시 황토살포 30분후에 Cochlodinium 적조생물이 $72\~83\%$가 제거됨으로써 좋은 제거효과를 나타냈다. 수질인자중 수온과 pH는 살포전후 변화가 없었으나 용존산소, 화학적산소요구량 및 클로로필 a의 농도는 다소 감소하였고 부유물질은 증가하였다. 부영양화 인자중 용존무기인은 살포전후 농도변화가 없는 반면, 용존무기질소는 살포후가 살포전에 비해 다소 감소하였다. 따라서 황토살포는 $10\;g/{\ell}$전후로 살포하는 것이 적조생물 제거효과에 바람직하며 수질에도 큰 영향이 없는 것으로 조사되었다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2020년도 학술발표회
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• pp.231-231
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• 2020

Unlike conventional treatment technologies, the performance of nature-based facilities were susceptible to seasonal changes and climatological variabilities. This study evaluated the effects of seasonal variables on the treatment performance of constructed wetlands (CWs). Two CWs treating runoff and discharge from agricultural and livestock areas were monitored to determine the efficiency of the systems in reducing particulates, organics, and nutrients in the influent. For all four seasons, the mean effluent suspended solids concentration in the agricultural CW (ACW) increased by -2% to -39%. The occurrence of algal blooms in the system during summer and fall seasons resulted to the greatest increase in the amount of suspended materials in the overlying water. unlike ACW, the livestock CW (LCW) performed efficiently throughout the year, with mean suspended solids removal amounting to 61% to 68%. Algal blooms were still present in LCW seasonally; however, the constant inflow in the system limited the proliferation of phytoplankton through continuous flushing. The total nitrogen (TN) and total phosphorus (TP) removal efficiencies in ACW were higher during the summer (21% to 25%) and fall (8% to 21%) seasons since phytoplankton utilize nitrogen and phosphorus during the early stages of phytoplankton blooms. In the case of LCW, the most efficient reduction in TN (24%) and TP (54%) concentrations were also noted in summer, which can be attributed to the favorable environmental conditions for microbial activities. The mean removal of organics in ACW was lowest during summer season (-52% to 35%), wherein the onset of algal decay triggered a relative increase in organic matter and stimulate bacterial growth. The removal of organics in LCW was highest (54 % to 55%) during the fall and winter seasons since low water temperatures may limit the persistence of various algal species. Variations in environmental conditions due to seasonal changes can greatly affect the performance of CW systems. This study effectively established the contributory factors affecting the feasibility of utilizing CW systems for treating agricultural and livestock discharges and runoff.

• 한국관개배수논문집
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• 제18권1호
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• pp.33-43
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• 2011

Jundae reservoir has basin area of 234ha, average depth of 3.77m and total storage of $619{\times}10^3m^3$, and is located in Dangin-gun, Chungcheongnam-do. The water quality of Jundae reservoir exceeded the IV grade of water quality standard as available for irrigation water in COD, TN, TP, Chl-a. COD and Chl-a were higher in spring season, because the algal bloom by phytoplankton increased. And the algal blooms in October by inflow non-point pollution during summer rainy season. The most dominant zooplankton was rotifers during study period at all stations. Dominant species were Keratella cochlearis, Polyarthra spp., and Trichocerca spp. We successfully established 2 isolated clone cultures as predator. One is Rotifer, Euchlanis sp. and another is cladocerans, Bosmina sp. To test the removal rate of 2 cultures against Microcystis aeruginosa, we inoculated Euchlanis sp. and Bosmina sp. separately when the abundance reached at $1.0{\times}10^6$cells/ml. Euchlanis sp. removed M. aeruginosa around 98.9% and Bosmina sp. removed it around 98.4%. They are useful grazers for controling algae blooms, Euchlanis sp. and Bosmina sp. feeding on M. aeruginosa highly.

• 한국해양공학회지
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• 제22권5호
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• pp.7-24
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• 2008

Masan-Jinhae Bay, in Korea, is known for its frequent algal bloom outbreaks. This study was conducted in order to examine the environmental characteristics of the area, with the aim of identifying indicators that could be used to speculate about future algal blooms. The water temperatures and salinities in Haengam Bay, one of the small inner bays within Jinhae, appeared to re relatively higher than those in Masan and Jinhae bays, across most seasons. Furthermore, stratification begins to develop in all three regions from spring to summer as a result of the local heating effects and an increase in the efficient from the surrounding land. As a result, anoxic conditions appear near the bottom layer of the bay, leading to the deterioration of water quality, which has been identified as one of the causes of bloom outbreaks. Compared to Haengam and Jinhae bays, concentrations of DIN and DIP were remarkably higher in Masan Bay. However, the mean ratio of DIN to DIP was 3.3$\sim$13.6 in all three regions throughout the year, suggesting that nitrogen can function as a growth-limiting factor for phytoplankton. The results of mathematical models showed that cumulative organic pollutants may be a trigger for direct algal bloom occurrences, since residual tidal currents appeared to be less than $3\;cm\;\cdot\;s^{-1}$. Furthermore, computed DO concentrations in the four small inner bays of Jinhae during the summer appeared to be $3\;cm\;\cdot\;l^{-1}$ indicating a hypoxic state. Likewise, computed Chl-a concentrations turned out to be more than $0.01\;mg\;\cdot\;l^{-1}$, indicating eutrophication across most seasons. Based on the overall results, Masan-Jinhae Bay appeared to possess a very high potential for algal bloom outbreaks at anytime during the year.

• 한국물환경학회지
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• 제31권2호
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• pp.110-119
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• 2015

The construction of the eight large weirs in the Nakdong River, the second largest river in Korea, caused big changes in the physical environment of the water system. Algal communities and their correlations with environmental factors, mainly nutrients were studied at five weir areas in the Nakdong River from 2010 to 2013. Water quality, hydrodynamics and algal composition were investigated. Results showed that flow velocities were reduced compared with that before weir construction near the areas where are located in the mid and upstreams of the Nakdong River. A seasonal algal community succession was observed and it was mainly correlated with temperature and phosphorus. Diatoms were dominated from winter to spring months and massive diatomic blooms of Stephanodiscus sp. occurred early in March during survey period. Cyanobacterial blooms of Microcystis sp. occurred from July to September 2013 and was preceded by the lower total phosphorus concentration of $0.05mg\;L^{-1}$. The correlations between total phosphorus concentrations and algal abundances were not significant during the survey periods. However, significant correlation with cyanobacteria was found in the period of weir construction after only at the GG survey site and blooms periods of 7 times in the survey sites, and its correlation coefficients were 0.53 (p<0.001) and 0.42 (p<0.01) respectively. When algal bloom was observed, partially low nutrient concentration was observed in the Nakdong River. In conclusion, partially low nutrient concentration which may result from algal bloom was observed, and we presume it caused the reduction of algal abundunces.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2016년도 정기학술대회 발표논문집
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• pp.183-183
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• 2016

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume I
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• pp.441-444
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• 2006

The MERIS MCI (Maximum Chlorophyll Index), measuring the radiance peak at 709 nm in water-leaving radiance, indicates the presence of a high surface concentration of chlorophyll ${\underline{a}}$ against a scattering background. The index is high in 'red tide' conditions (intense, visible, surface, plankton blooms), and is also raised when aquatic vegetation is present. A bloom search based on MCI has resulted in detection of a variety of events in Canadian, Antarctic and other waters round the world, as well as detection of extensive areas of pelagic vegetation (Sargassum spp.), previously unreported in the scientific literature. Since June 1 2006, global MCI composite images, at a spatial resolution of 5 km, are being produced daily from all MERIS (daylight) passes of Reduced Resolution (RR) data. The global composites significantly increase the area now being searched for events, though the reduced spatial resolution may cause smaller events to be missed. This paper describes the composites and gives examples of plankton bloom events that they have detected. It also shows how the composites show the effect of the South Atlantic Anomaly, where cosmic rays affect the MERIS instrument.

• 한국수산과학회지
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• 제36권2호
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• pp.178-186
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• 2003

Seasonal variation of phytoplankton was investigated with surface mixed layer ecosystem model in the East Sea. The model consisted of four compartments (phytoplankton, zooplankton, nutrient, detritus) forced by mixed layer depths, photosynthetically available radiation and nutrient concentrations. From model results we estimated entrainment rate $2.5-4.0\;m{\cdot}day^{-1}$ to reproduce the two annual blooms, and reproduced seasonal variation of phytoplankton at southern and northern regions by the difference of surface winter mixed layer depth (MLD) using the entrainment rate value $3.0\;m{\cdot}day^{-1}$. The spring blooms in the southern and northern regions closely related to deepening of a winter surface MLD. In the southern region where MLD was shallow and phytoplankton spring bloom occurs one month in advance to the northern region where MLD was deep. The amount of light increases within the MLD during the onset of stratification and water temperature increases faster in spring in the southern region than the northern region. Decrease of phytoplankton was mainly affected by zooplankton grazing in the southern region and by nutrient exhaustion in the northern region. The fall bloom in the two regions was caused by the nutrient availability and entrainment on the phytoplankton.

• 한국물환경학회지
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• 제33권4호
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• pp.441-448
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• 2017

Microcystis aeruginosa (M. aeruginosa) is a cyanobacterium species that can form harmful algal blooms in freshwater bodies worldwide. The use of Artemisia asiatica extracts to control M. aeruginosa inhibition will be environmentally friendly and promising. Artemisia asiatica extracts removed successfully upto 88% of M. aeruginosa pH 8 at $25^{\circ}C$ of temperature. These results was indicated that the amount of 2.24 g/L Artemisia asiatica extracts was removed 1g dryweight/L of M. aeruginosa. The kinetic data showed substrate inhibition kinetics and maximum growth rate was obtained when the M. aeruginosa was grown in medium containing 2.5 g/L of initial concentration of Artemisia asiatica extracts. In the various growth control models, Luong model showed the highest correlation coefficient of 0.9916. Therefore, the Luong model was the most suitable control model for the growth control of M. aruginosa using Artemisia asiatica extracts. In conclusion, the growth control of M. aruginosa using Artemisia asiatica extracts can be applied in the field without controlling the temperature and pH of rivers and streams, and it is possible to control the growth of M. aruginosa efficiently in a short time. The natural extract, Artemisia asiatica extracts, can be a promising inhibition due to its high efficiency and low dose requirements.