• Title/Summary/Keyword: water surface

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Evaluation of Meteorological Elements Used for Reference Evapotranspiration Calculation of FAO Penman-Monteith Model (FAO Penman-Monteith 모형의 증발산량 산정에 이용되는 기상요소의 평가)

  • Hur, Seung-Oh;Jung, Kang-Ho;Ha, Sang-Keun;Kim, Jeong-Gyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.5
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    • pp.274-279
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    • 2006
  • The exact estimation of crop evapotranspiration containing reference or potential evapotranspiration is necessary for decision of crop water requirements. This study was carried out for the evaluation and application of various meteorological elements used for the calculation of reference evapotranspiration (RET) by FAO Penman-Monteith (PM) model. Meteorological elements including temperature, net radiation, soil heat flux, albedo, relative humidity, wind speed measured by meteorological instruments are required for RET calculation by FAO PM model. The average of albedo measured for crop growing period was 0.20, ranging from 0.12 to 0.23, and was slightly lower than 0.23. Determinant coefficients by measured albedo and green grass albedo were 0.97, 0.95 and standard errors were 0.74, 0.80 respectively. Usefulness of deductive regression models was admitted. To assess an influence of soil heat flux (G) on FAO PM, RET with G=0 was compared with RETs using G at 5cm soil depth ($G_{5cm}$) and G at surface ($G_{0cm}$). As the results, RET estimated by G=0 was well agreed with RET calculated by measured G. Therefore, estimated net radiation, G=0 and albedo of green grass could be used for RET calculation by FAO PM.

Mineralogical and Chemical Characteristics of the Oyster Shells from Korea (국내산 굴 패각의 광물학적 화학적 특성)

  • Ha, Su Hyeon;Cha, Min Kyung;Kim, Kangjoo;Kim, Seok-Hwi;Kim, Yeongkyoo
    • Journal of the Mineralogical Society of Korea
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    • v.30 no.4
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    • pp.149-159
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    • 2017
  • We investigated the mineralogical and chemical characteristics of oyster shell as the possible substitute for the limestone used as an absorbent of $SO_2$ gas. The oyster shells from Taean and Tongyeong were used for the comparison with limestone and those from Boyreong and Yeosu were additionally investigated. XRD results show that all shells are composed of calcite with the exception of the myostracum layer attached to adductor muscle and ligament, which is composed of aragonite. The marine sediments as impurities exist on the surface of shells or as inclusions in the shells. Calcite is the main mineral composition of the shell of barnacle which is also one of the impurities. The oyster shell is composed of three main layers; prismatic, foliated, and chalk. The oyster shell from Tongyeong with the largest shell size, has the smallest thickness of prismatic and foliated layers which contain protein called conchiolin, whereas that from Taean with the smallest shell size has the largest prismatic and foliated layers. The sizes of those two layers of the shells from Boryeong and Yeosu are larger than that from Tongyeong but smaller than Taean. Those differences are supposed to be due to the different growth environments because the oysters from Tongyeong are cultured under the sea while those from Taean are in the tidal zone. The oyster shells generally show higher amount of sulfur and phosphorus than limestone, mainly due to the composition of protein. Some elements such as Mg show significant variations in different layers. As for trace elements, Li shows much higher amount in oyster shells than limestone, suggesting the influence of the composition of the sea water on the formation of the oyster shells.

Convenient Nucleic Acid Detection for Tomato spotted wilt virus: Virion Captured/RT-PCR (VC/RT-PCR) (Tomato spotted wilt virus를 위한 간편한 식물바이러스 핵산진단법: Virion Captured/RT-PCR (VC/RT-PCR))

  • Cho Jeom-Deog;Kim Jeong-Soo;Kim Hyun-Ran;Chung Bong-Nam;Ryu Ki-Hyun
    • Research in Plant Disease
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    • v.12 no.2
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    • pp.139-143
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    • 2006
  • Virion captured reverse transcription polymerase chain reaction (VC/RT-PCR) could detect plant virus quickly and accurately. In the VC/RT-PCR, no antibody is needed unlike immuno-captured RT-PCR (IC/RT-PCR) which had been improved method of RT-PCR for plant viruses, and virus nucleic acids can be obtained easily within 30minutes by property of polypropylene PCR tube which is hold and immobilized viral particles on its surface. For the virion capture of Tomato spotted wilt virus (TSWV), the extraction buffer was tested. The optimum macerating buffer for TSWV was 0.01M potassium phosphate buffer, pH 7.0, containing 0.5% sodium sulfite. The viral crude sap was incubated for 30 min at $4^{\circ}C$. The virions in the PCR tubes were washed two times with 0.01M PBS containing 0.05% Tween-20. The washed virions were treated at $95^{\circ}C$ immediately for 1 min containing RNase free water and chilled quickly in the ice. Disclosed virions' RNAs by heat treatment were used for RT-PCR. Dilution end point of $10^{-5}$ from plant's crude sap infected with TSWV showed relatively higher detection sensitivity for VC/RT-PCR. During multiple detection using two or more primers, interference was arisen by interactions between primer-primer and plant species. The result of multiplex RT-PCR was influenced by combinations of primers and the kind of plant, and the optimum extraction buffer for the multiplex detection by VC/RT-PCR should be developed.

Analysis of Clonorchis sinensis antigens and diagnosis of clonorchiasis using monoclonal antibodies (단세포군 항체를 이용한 간흡충 항원의 분석 및 간흡충증의 진단)

  • Yong, Tae-Sun;Im, Gyeong-Il;Jeong, Pyeong-Rim
    • Parasites, Hosts and Diseases
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    • v.29 no.3
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    • pp.293-310
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    • 1991
  • Clonorchis sinensis is a common parasite of man in Korea. Researches on the specific antigens of C. sinensis would be valuable not only because those elucidate the molecular characteristics of this fluke but also because it is applicable to immunodiagnosis. Although many monoclonal antibodies have been used in the field of parasite immunology, few articles on monoclonal antibodies against C. sinensis have been published so far. The aim of this study was to analyse C. sinensis antigens recognized by monoclonal antibodies, and to set up ELISA-inhibition test using C. sinensis specific monoclonal antibodies for improved specificity of immunodiagnostic tests. By fusion between spleen cells of the mice immunized with C. sinensis water-soluble crude adult worm antigens and plasmacytoma cells of mouse origin, 29 hybridoma clones secreting anti-C. sinensis monoclonal antibodies were made, and 8 clones among those were found specific. After cell cloning, isotypes of 6 selected specific monoclonal anti- bodies were determined to be IgGl, IgG2b and IgA. Four exposed antigenic determinants of natural infection were recognized by different specific monoclonal antibodies. By enzyme-immunoelectrotransfer blot, 10 KD, 34 KD antigenic determinants were found to be reacted with CsHyb 0714-20, CsHyb 0605-10 monoclonal antibodies, respectively, The antigenic determinant recognized by CsHyb 0714-20 monoclonal antibody was revealed to be located at the surface and parenchyme of a parasite by indirect immunoauorescent antibody technique, and those reacted with CsHyb 0605-10, CsHyb 0714-25 monoclonal antibodies were found at the parenchyme and intestine. The antigenic determinant reacted with CsHyb 0605-23 monoclonal antibody was found mainly around the uterine eggs. Four antigenic determinants recognized by specific monoclonal antibodies were all found to be present in the early eluted fractions of C. sinensis antigens separated by Sephadex G-200 gel filtration. By conventional ELISA, 75% of clonorchiasis cases were found positive, but 7.1% of normal controls and 37.5% of paragonimiasis cases showed false positives. However, by ELISA-inhibition test using C. sinensis specific monoclonal antibody (CsHyb 0605-23), 77.1% of clonorchiasis cases were found positive, and there were no false positives in normal controls or paragonimiasis cases, indicating 100% specificity. The ELISA- inhibition test using monoclonal antibodies was found to have same sensitivity and definitely high specificity in comparison with conventional ELISA for serodiagnosis of human clonorchiasis.

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Combined Effects of Filter-feeding Bivalve and Zooplankton on the Growth Inhibition of Cyanobacterium Microcystis aeruginosa (남세균 제어를 위한 동물플랑크톤(Daphnia magna)과 패류(Unio douglasiae)의 단독 및 혼합적용)

  • Kim, Nan-Young;Park, Myung-Hwan;Hwang, Su-Ok;Kim, Baik-Ho;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.48 no.2
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    • pp.108-114
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    • 2015
  • Single - and combined effects of a domestic freshwater bivalve Unio douglasiae (7.6~8.6 cm in shell length) and zooplankton Daphnia magna (1~2 mm in body size) were examined to understand whether they inhibit the growth of harmful cyanobacterial bloom (i.e. Microcystis aeruginosa) in a eutrophic lake. The experiments were triplicated with twelve glass aquaria (40 L in volume); three aquaria without mussel and zooplankton, served as a control, three zooplankton aquaria (Z, density=40 indiv. $L^{-1}$), three mussel aquaria (M, density=0.5 indiv. $L^{-1}$), and three mussel plus zooplankton aquarium (ZM, density=40 indiv.Z $L^{-1}$ plus 0.5 indiv.M/L), respectively. Algal growth inhibition (%) calculated as a difference in the concentration of chlorophyll-a (Chl-a) before and after treatment. Chl-a in all aquaria decreased with the time, while a greatest algal inhibition was seen in the ZM aquaria. After 24 hrs of incubation, Chl-a concentration at the mid-depth (ca. 15 cm) in ZM aquaria reduced by 90.8% of the control, while 63.2% and 79.8% in Z and M aquaria, respectively. Interestingly, during the same period, the surface Chl-a was diminished by 51.9% and 65.4% relative to the control in Z and ZM aquaria, while 27.4% of initial concentration decreased in M aquarium, respectively. These results suggest that 1) this domestic freshwater filter-feeding bivalve plays a significant role in the control of cyanobacterial bloom (M. aeruginosa), and 2) the combination with zooplankton and mussel has a synergistic effect to diminish them, compared to the single treatment of zooplankton and mussel.

Research Trend of Biomass-Derived Engineering Plastics (바이오매스 기반 엔지니어링 플라스틱 연구 동향)

  • Jeon, Hyeonyeol;Koo, Jun Mo;Park, Seul-A;Kim, Seon-Mi;Jegal, Jonggeon;Cha, Hyun Gil;Oh, Dongyeop X.;Hwang, Sung Yeon;Park, Jeyoung
    • Applied Chemistry for Engineering
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    • v.31 no.2
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    • pp.115-124
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    • 2020
  • Sustainable plastics can be mainly categorized into (1) biodegradable plastics decomposed into water and carbon dioxide after use, and (2) biomass-derived plastics possessing the carbon neutrality by utilizing raw materials converted from atmospheric carbon dioxide to biomass. Recently, biomass-derived engineering plastics (EP) and natural nanofiber-reinforced nanocomposites are emerging as a new direction of the industry. In addition to the eco-friendliness of natural resources, these materials are competitive over petroleum-based plastics in the high value-added plastics market. Polyesters and polycarbonates synthesized from isosorbide and 2,5-furandicarboxylic acid, which are representative biomass-derived monomers, are at the forefront of industrialization due to their higher transparency, mechanical properties, thermal stability, and gas barrier properties. Moreover, isosorbide has potential to be applied to super EP material with continuous service temperature over 150 ℃. In situ polymerization utilizing surface hydrophilicity and multi-functionality of natural nanofibers such as nanocellulose and nanochitin achieves remarkable improvements of mechanical properties with the minimal dose of nanofillers. Biomass-derived tough-plastics covered in this review are expected to replace petroleum-based plastics by satisfying the carbon neutrality required by the environment, the high functionality by the consumer, and the accessibility by the industry.

A Study on Physical Properties About Velvet Antler of the Elk(Cerus canadensis) (엘크 녹용의 물성 특성에 관한 연구)

  • Sung, S.H.;Han, J.H.;Kim, Y.M.;Kim, B.K.
    • Journal of Animal Environmental Science
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    • v.13 no.3
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    • pp.201-210
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    • 2007
  • In this study, physical properties of Elk antler was investigated to develop the optimum drying and packaging methods for improving the antler quality as well as deal with diversify of demand. After the antler was sliced with 5 mm thickness, and the compressive, shear, and tensile stresses were measured at the center and velvet parts of pre-dried and dried antlers after the contained water rate of the dried antlers was maintained below 10%. The results are as follows. 1. Considering the center of pre-dried antlers, the compressive stresses were $60.73\;g/mm^2$, $145.65\;g/mm^2$, and $260.97\;g/mm^2$, respectively at the upper, middle, and lower parts while $70.67\;g/mm^2$, $811.90\;g/mm^2$, $3,235.52\;g/mm^2$, respectively for velvet layer. Considering the center of dried antlers, the compressive stresses were $190.43\;g/mm^2$, $445.81\;g/mm^2$, and $705.86\;g/mm^2$, respectively at the upper, middle, and lower parts while $734.01\;g/mm^2$, $1,238.40\;g/mm^2$, $4,134.03\;g/mm^2$, respectively for velvet layer. 2. For the pre-dried, the shear stresses were $50.24\;g/mm^2$, $294.44\;g/mm^2$, and $423.47\;g/mm^2$, respectively, and $124.14\;g/mm^2$, $367.69\;g/mm^2$, and $425.86\;g/mm^2$, respectively for the dried antlers. 3. The tensile stresses were $13.59\;g/mm^2$, $62.85\;g/mm^2$, and $112.07\;g/mm^2$, respectively for the pre-dried and $77.24\;g/mm^2$, $175.87\;g/mm^2$, and $184.06\;g/mm^2$, respectively for the dried antlers. 4. In the case of drying antlers, the physical characteristics of the antlers was. changed such as moisture evaporation, contraction, and surface hardening. For the center part, the changes of the physical characteristics were more significant at the lower part while at the upper part for the velvet layer. 5. The stress changes of Elk antlers was shown very remarkably according to growth point. Moreover, the stress was clearly higher at velvet layer part to the center part, base parts compared to the upper parts.

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Microbiological Hazard Analysis for HACCP System Application to Non Heat-Frozen Carrot Juice (비가열냉동 당근주스의 HACCP 시스템 적용을 위한 미생물학적 위해 분석)

  • Lee, Ung-Soo;Kwon, Sang-Chul
    • Journal of Food Hygiene and Safety
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    • v.29 no.2
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    • pp.79-84
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    • 2014
  • This study has been performed for about 270 days at analyzing biologically hazardous factors in order to develop HACCP system for the non heat-frozen carrot juice. A process chart was prepared by manufacturing process of raw agricultural products of non heat-frozen carrot juice, which was contained water and packing material, storage, washing, cutting, extraction of the juice, internal packing, metal detection, external packing, storage and consignment (delivery). As a result of measuring Coliform group, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Listeria Monocytogenes, Enterohemorrhagic E. coli before and after washing raw carrot, Standard plate count was $4.7{\times}10^4CFU/g$ before washing but it was $1.2{\times}10^2CFU/g$ detected after washing. As a result of testing airborne bacteria (Standard plate count, Coliform group, Yeast and Fungal) depending on each workplace, number of microorganism of in packaging room, shower room and juice extraction room was detected to be 10 CFU/Plate, 60 CFU/Plate, 20 CFU/Plate, respectively. As a result of testing palm condition of workers, as number of Standard plate count, Coliform group and Staphylococcus aureus was represented to be high as $6{\times}10^4CFU/cm^2$, $0CFU/cm^2$ and $0CFU/cm^2$, respectively, an education and training for individual sanitation control was considered to be required. As a result of inspecting surface pollution level of manufacturing facility and devices, Coliform group was not detected in all the specimen but Standard plate count was most dominantly detected in scouring kier, scouring kier tray, cooling tank, grinding extractor, storage tank and packaging machine-nozzle as $8.00{\times}10CFU/cm^2$, $3.0{\times}10CFU/cm^2$, $4.3{\times}10^2CFU/cm^2$, $7.5{\times}10^2CFU/cm^2$, $6.0{\times}10CFU/cm^2$, $8.5{\times}10^2CFU/cm^2$ respectively. As a result of analyzing above hazardous factors, processing process of ultraviolet ray sterilizing where pathogenic bacteria may be prevented, reduced or removed is required to be controlled by CCP-B (Biological) and critical level (critical control point) was set at flow speed is 4L/min. Therefore, it is considered that thorough HACCP control plan including control criteria (point) of seasoning fluid processing process, countermeasures in case of its deviation, its verification method, education/training and record control would be required.

COMPARATIVE ENAMEL BOND STRENGTH BETWEEN LIGHT-AND DUAL-CURED COMPOSITES BONDED BY SELF-ETCHING ADHESIVES (자가 산부식 접착제로 접착된 광중합과 이원중합 복합레진의 법랑질 결합강도 비교)

  • Cho, Young-Gon;Yoo, Sang-Hoon
    • Restorative Dentistry and Endodontics
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    • v.32 no.1
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    • pp.1-8
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    • 2007
  • This study compared the microshear bond strength (${\mu}SBS$) of light-cured and dual-cured composites to enamel bonded with three self-etching adhesives. Crown segments of extracted human molars were cut mesiodistally, and 1 mm thickness of specimen was made. They were assigned to three groups by used adhesives: Xeno group (Xeno III), Adper group (Adper Prompt L-Pop), and AQ group (AQ Bond). Each adhesive was applied to cut enamel surface as per manufacturer's instruction. Light-cured (Filtek Z 250) or dual-cured composite (Luxacore) was bonded to enamel of each specimen using Tygon tube. After storage in distilled water for 24 hours, the bonded specimens were subjected to ${\mu}SBS$ testing with a crosshead speed of 1 mm/minute. The mean ${\mu}SBS$ (n = 20 for each group) was statistically compared using two-way ANOVA, Tukey HSD, and t test at the 0.05 probability level, The results of this study were as follows ; 1. The ${\mu}SBS$ of light-cured composite was significantly higher than that of dual-cured composite when same adhesive was applied to enamel. 2. For Z 250, the ${\mu}SBS$ of AQ group ($9.95{\pm}2.51 MPa$) to enamel was significantly higher than that of Adper soup ($6.74{\pm}1.80 MPa$), but not significantly different with Xeno group ($7.73{\pm}2.01 MPa$). 3. For Luxacore, the ${\mu}SBS$ of Xeno group ($5.19{\pm}1.32\;MPa$) to enamel was significantly higher than that of Adper group ($3.41{\pm}1.19\;MPa$), but not significantly different with AQ group ($4.50{\pm}0.96\;MPa$).

Investigations on the Adsorption Characteristics of $SO_2$ Gas on Fixed Bed Manganese Nodule Column (고정(固定) 흡착층(吸着層)에서 망간단괴(團塊)의 $SO_2$ 가스 흡착(吸着) 특성(特性)에 관한 연구(硏究))

  • Baek, Mi-Hwa;Kim, Dong-Su;Jung, Sun-Hee;Park, Kyoung-Ho
    • Resources Recycling
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    • v.15 no.4 s.72
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    • pp.3-12
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    • 2006
  • The feasibility for the employment of manganese nodule as an adsorbent for $SO_{2}$ gas has been investigated. The specific surface area of manganese nodule particle, which used in the experiments, was ca. $221.5m^{2}/g$ and the content of sulfur in manganese nodule was observed to significantly increase after $SO_{2}$ was adsorbed on it. The EPMA for the distilled water-washed and methanol-washed manganese nodule particle after $SO_{2}$ adsorption showed that its sulfur content was slightly decreased to 14.7% and 13.1% respectively, from 15.4% before washing. The XRD analysis of manganese nodule showed that todorokite and birnessite, which are manganese oxides, and quartz and anorthite were the major mineralogical components and weak $MnSO_{4}$ peaks were detected after $SO_{2}$ was adsorbed on manganese nodule. For an comparative investigation, limestone was also tested as an adsorbent for $SO_{2}$, however, no peaks for $CaSO_{4}$ were found by XRD analysis after the adsorption of $SO_{2}$. As the size of adsorbent increased, time for breakthrough was decreased and the adsorbed amount of $SO_{2}$ was also diminished. The $SO_{2}$ adsorption was hindered when its flow rate became high and the adsorption capacity of manganese nodule was observed to be superior to that of limestone. In addition, the mixture of manganese nodule and limestone did not show an increase in the adsorption of $SO_{2}$. Finally, as the temperature was raised, the adsorbed amount of adsorbate on manganese nodule was found to be decreased.