• Journal of Sensor Science and Technology
• /
• v.29 no.2
• /
• pp.93-99
• /
• 2020

A colorimetric paper sensor was used to detect volatile nitrogen-containing compounds emitted from spoiled salmon filets to determine their freshness. The sensing mechanism was based on acid-base reactions between acidic pH-indicating dyes and basic volatile ammonia and amines. A sensing layer was simply fabricated by drop-casting a dye solution of bromocresol green (BCG) on a polyvinylidene fluoride substrate, and its color-change response was enhanced by optimizing the amounts of additive chemicals, such as polyethylene glycol, p-toluene sulfonic acid, and graphene oxide in the dye solution. To avoid the adverse effects of water vapor, both faces of the sensing layer were enclosed by using a polyethylene terephthalate film and a gas-permeable microporous polytetrafluoroethylene sheet, respectively. When exposed to basic gas analytes, the paper-like sensor distinctly exhibited a color change from initially yellow, then to green, and finally to blue due to the deprotonation of BCG via the Brønsted acid-base reaction. The use of ammonia analyte as a test gas confirmed that the sensing performance of the optimized sensor was reversible and excellent (detection time of < 15 min, sensitive naked-eye detection at 0.25 ppm, good selectivity to common volatile organic gases, and good stability against thermal stress). Finally, the coloration intensity of the sensor was quantified as a function of the storage time of the salmon filet at 28℃ to evaluate its usefulness in monitoring of the food freshness with the measurement of the total viable count (TVC) of microorganisms in the food. The TVC value increased from 3.2 × 10⁵ to 3.1 × 10⁹ cfu/g in 28 h and then became stable, whereas the sensor response abruptly changed in the first 8 h and slightly increased thereafter. This result suggests that the colorimetric response could be used as an indicator for evaluating the degree of decay of salmon induced by microorganisms.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.35 no.3
• /
• pp.248-253
• /
• 1990

Germination and seedling growth of chinese milkyetch (Astragalus sinicus L. Line A) seeds were measured at four temperatures (15. 25. 30, and 35$^{\circ}C$), three polyethylene glycol (PEG) 10.000 (MW) solutions (0, 20 and 25 g/100$m\ell$ H$_2$O), and five sulfuric acid treatments (conc, H$_2$SO$_4$: Seeds/weight base: 0, 1:1 10min.. 1:1 20min.. 1:1.5 10min. and 1:1.5 20min, immersion), Adjustments of PEG 10.000 solutions were based on water potentials of 0.0, -0,5 and -0.8 ㎫ at room temperature. Observations were taken at 7 days for each treatment combinations. Germination percent of chinese mikvetch seeds was the highest at 15$^{\circ}C$ and seeding length was the longest at 25$^{\circ}C$. Both germination percent and seedling length were rapidly decreased as PEG concentration increased from 0 to 25g/100$m\ell$ H$_2$O. The highest germination percent was found at PEG 20 and 25 of 15$^{\circ}C$ among those of four temperatures, Sulfuric acid treatments showed little effect on germination percent and seedling length compared to untreated control, However. in the case of PEG 0, greater germination perccent was obtained at the treatment of 1:1.5 for 10 minutes immersion, The result of this study-indicated that germination of chinese milkvetch seeds was affected mostly by moisture stress conditions.

• Journal of Life Science
• /
• v.9 no.2
• /
• pp.169-175
• /
• 1999

Salmonella typhimurium can encounter a wide variety of environments during its life cycle. In nature, S. typhimurium can experience and survive dramatic acid stresses that occur in diverse ecological niches ranging from pond water to phagolysosomes. These survival mechanism is aquired by the Acid Tolerance Response(ATR) in Salmonella. The ATR of S. typhimurium is a complex inducible phenomenon in which exposures to slight or moderate low pH will produce a stress response capable of protecting the organism against more severe acid challenges. ATR in Salmonella has two different systems that are called RpoS dependent and independent. We found that ATR in anaerobic was showed RpoS independent because rpoS$\Omega$AP had ATR as S. typhimurium UK1. Using the P22 MudJ(Km, lacZ) operon fusion technique and a lethal selection procedure combining low pH(pH4.5) and sodium acetate(10mM, pH4.5), we isolated LF487 aatA::MudJ which showed acid sensitive in anaerobic condition. aatA locus was determined at 12 min on Salmonella Genetic Map. The survival rate of aatA mutant was showed significantly diminished at pH4.3 than virulent wild type Salmonella in anaerobic condition(5% $CO_2$, 5% H$_2$, 90% $N_2$). Therefore isolated gene was confirmed important gene for anaerobic ATR system.

• Journal of Life Science
• /
• v.9 no.2
• /
• pp.231-240
• /
• 1999

Regular exercise is effective in preventing coronary disease such as angina pectoris and infarction, inside it can lower the blood pressure and aids in weight control and release of stress. Risk factors of arteriosclerosis is hypertension, hyperlipidemia, diabetes, obesity, physical inactivity and excessive smoking. Arteriosclerosis begins at young age worsens with age, particulary in male. For people with risk factors of arteriosclerosis, it is important to prevent arteriosclerosis-related disease with dietary, living pattern and exercise prescription. Dietary fibers promote exercise of the digestive tract and shortens the time food remains inside the digestive tract. It can prevent obesity, hyperlipidemia, arteriosclerosis and colin cancer by blocking the absorption of cholesterol. Various vegetables and sea foods are lichen unsaturated fats and prevent the absorption of cholesterol inside the digestive tract. Essential fatty acids and unsaturated fats which are contained in vegetable oils, promotes metabolism while preventing absorption. In fruits, pectin water-soluble fiber, is present and lowers the level of cholesterol. By consuming foods that low in cholesterol and saturated fats, and rich in unsaturated fats, aliomentotherapy alone can reduce the plasma cholesterol by 10~l5$\%$. For ideal exercise, it should be aerobic with intensity of 60~80$\%$ HRmax, duration of 15~60min/day. The frequancy of 3~6/week is desirable the better exercise prescription is endurance aerobic exercise. To get more effect exercise, exercise consistency is very important.

• Tunnel and Underground Space
• /
• v.10 no.4
• /
• pp.526-532
• /
• 2000

The uniaxial compressive strength of rock mass is known as the major factor in the assessment of drillability and the optimum excavation design in full-face tunnel excavation by TBM. Referring to worldwide cases, TBM has been applied mostly to the rock mass within the strength range of 80~250 MPa. Recently, a water way tunnel has been constructed as a part of Milyang dam project by TBM within the rock masses where the rock type is mainly granite with some granophyre, hornfels and andesite. Their uniaxial compressive strengths in extended area are estimated higher than 260 MPa. In this paper, the relation between the penetration rate and the rock mass properties is analyzed and TBM application to the very hard rocks is discussed. As a result that three suggestions to predict the TBM net penetration rate are analyzed, NTH method seems a better approach than other methods in the extremely hard rocks. NTH prediction matches with the results of actual values with the variations of 2~20%. Hardness measurement by Schmidt hammer and RMR estimation are carried out along the L = 5.3 km entire TBM tunnel alignment. The net penetration rate measured monthly is shown to be reciprocally proportional to Schmidt rebound hardness and RMR where coefficients of correlation, $R^2$are 0.705 and 0.777 respectively. As a result, they are good quantitative indices for the prediction of TBM net penetration rate in the extremely hard rocks. Magnitude of in-situ stress has a certain effect on TBM performance, and it is required to measure the in-situ stresses in TBM excavation design.

• Tunnel and Underground Space
• /
• v.19 no.3
• /
• pp.236-247
• /
• 2009

Overpopulation has significantly increased the use of underground spaces in urban areas, and led to the developments of shallow-depth underground space. Due to unexpected rock fall, however, it is very necessary to understand and categorize the rock mass behaviors prior to the tunnel excavation, by which unnecessary casualties and economic loss could be prevented. In case of cave-in, special attention should be drawn since it occurs faster and greater in magnitude compared to rock fall and plastic deformation. Types of cave-in behavior are explained and categorized using seven parameters - Uniaxial Compressive Strength (UCS), Rock Quality Designation (RQD), joint surface condition, in-situ stress condition, ground water condition, earthquake & ground vibration, tunnel span. This study eventually introduces a new index called Cave-in Behavior Index (CBI) which explains the behavior of cave-in under given in-situ conditions expressed by the seven parameters. In order to assess the mutual interactions of the seven parameters and to evaluate the weighting factors for all the interactions, survey data of the experts' opinions and Rock Engineering Systems (RES) were used due to lack of field observations. CBI was applied to the tunnel site of Seoul Metro Line No. 9. UDEC analyses on 288 cases were done and occurrences of cave-in in every simulation were examined. Analyses on the results of 288 cases of simulations revealed that the average CBI for the cases when cave-in for different patterns of tunnel support was estimated by a logistic regression analysis.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
• /
• 2001.06a
• /
• pp.1256-1256
• /
• 2001

The physico-chemical and texture characteristics of meat determine the nutritional, technological and sensory quality. However, the analysis of meat quality requires expensive, laborious and time consuming analytical methods. The objective of this study was to evaluate NIR spectroscopy using transmittance for determining the moisture, fat, protein and total pigment content, the water holding capacity (WHC) and the toughness of beef meat. A total of 318 spectra were recorded from ground beef samples by a Feed Analyzer 1265 of Infratec. The samples were obtained from the Longissimus muscle of the 10$^{th}$ rib of yearling bulls, ground with an electrical chopper, vacuum packaged, aged during 7 days and frozen at -24$^{\circ}C$ until the analyses were done. Moisture content was measured by oven drying at 10$0^{\circ}C$, fat content was determined by Soxhlet extraction and protein content was estimated from nitrogen content using the Kjeldahl analysis. The total pigment content was determined by the method of Hornsey and the WHC using the method of filter paper press. The instrumental evaluation of texture (maximum load WB, maximum stress MS and toughness) was conducted in an Instron equipment with a Warner-Bratzler shearing device. This analysis was performed on a chop of 3.5 cm obtained from the longissimus of the 8$^{th}$ rib, aged during 7 days, kept frozen at -24$^{\circ}C$ and cooked before the analysis. Near infrared spectra were recorded as log 1/T (T=transmittance) at 2 nm intervals from 850 to 1050 nm using a Feed Analyzer 1265 of Infratec. Calibrations were performed with the WinISI software (vs. 1.02) using the MPLS method. To examine the effect of scatter correction o. derivation of spectra on the calibration performance, calibrations were calculated with the crude spectra or pretreated with different mathematical treatments (inverse MSC, SNVD) and/or second derivative operation. For chemical composition, the use of the scatter corrections improved the calibration statistics, in terms of lower SECV and higher $r^2$. In most of the variables, the use of the 2$^{nd}$ derivative improved the predictions, mainly when combined with the SNVD treatment. However, for predicting the texture traits, the best estimation was obtained from the crude spectrum. These results showed that the equations obtained for predicting moisture, fat and total pigments were very accurate, with $r^2$ being higher that 0.9. However, the prediction of the texture traits (WB, MS, toughness) from ground meat was poor.

• Restorative Dentistry and Endodontics
• /
• v.35 no.1
• /
• pp.51-58
• /
• 2010

Dental composites have improved significantly in physical properties over the past few decades. However, polymerization shrinkage and stress is still the major drawback of composites, limiting its use to selected cases. Much effort has been made to make low shrinking composites to overcome this issue and silorane-based composites have recently been introduced into the market. The aim of this study was to measure the volumetric polymerization shrinkage kinetics of a silorane-based composite and compare it with conventional methacrylate-based composites in order to evaluate its effectiveness in reducing polymerization shrinkage. Five commercial methacrylate-based (Beautifil, Z100, Z250, Z350 and Gradia X) and a silorane-based (P90) composites were investigated. The volumetric change of the composites during light polymerization was detected continuously as buoyancy change in distilled water by means of Archemedes' principle, using a newly made volume shrinkage measurement instrument. The null hypothesis was that there were no differences in polymerization shrinkage, peak polymerization shrinkage rate and peak shrinkage time between the silorane-based composite and methacrylate-based composites. The results were as follows: 1. The shrinkage of silorane-based (P90) composites was the lowest (1.48%), and that of Beautifil composite was the highest (2.80%). There were also significant differences between brands among the methacrylate-based composites. 2. Peak polymerization shrinkage rate was the lowest in P90 (0.13%/s) and the highest in Z100 (0.34%/s). 3. The time to reach peak shrinkage rate of the silorane-based composite (P90) was longer (6.7 s) than those of the methacrylate-based composites (2.4-3.1 s). 4. Peak shrinkage rate showed a strong positive correlation with the product of polymerization shrinkage and the inverse of peak shrinkage time (R = 0.95).

• Herbal Formula Science
• /
• v.25 no.4
• /
• pp.509-526
• /
• 2017

Background and objectives : Soeumin Bojungykgi-tang (seBYTE) has been used to supplement qi in Korean medicine. It has been demonstrated to possess various biological functions such as anti-cancer, anti-aging and anti-inflammatory effects. The present study evaluated the protective roles of seBYTE in hepatotoxic in vitro and in vivo model. Methods : To investigate cytoprotective effect of seBYTE, HepG2 cells were pretreated with seBYTE and then subsequently exposed to $10{\mu}m$ AA for 12 h, followed by $5{\mu}m$ iron. Cell viability was examined by MTT assay, and expression of apoptosis-related proteins was evaluated by immunoblot analysis. For responsible molecular mechanisms, ROS production, GSH contents, and mitochondrial membrane potential were measured. In addition, hepatoprotective effect of seBYTE in vivo was assessed in $CCl_4$-induced animal model. Results : seBYTE prevented AA + iron-induced cytotoxicity in concentration dependent manner. In addition, ROS production, GSH depletion, and mitochondrial dysfunction induced by AA + iron were significantly reduced by seBYTE pretreatment. Furthermore, seBYTE recovered expression of the pro-apoptotic proteins such as PARP and pro-caspase-3. In animal experiment, plasma ALT and AST levels were significantly elevated in $CCl_4$ treatment, but seBYTE significantly decreased the ALT and AST levels. Moreover, seBYTE alleviated the numbers of histological activity index, percentages of degenerative regions, degenerated hepatocytes, infiltrated inflammatory cells, nitrotyrosine- and 4-hydroxynonenal-positive cells in liver. Conclusions : These results showed that hepatoprotective effect of seBYTE against on $CCl_4$-induced hepatic damages is partly due to antioxidative and anti-apoptotic process.

• Journal of fish pathology
• /
• v.20 no.2
• /
• pp.139-145
• /
• 2007

To decrease stress in eel (Anguilla japonica) during its culture or transportation, aspirin (ASA) known as analgesic, antiinflammatory and antithrombic agent was administrated by dipping or oral routes. Concentrations of aspirin (ASA) and salicylic acid (SA) in eel plasma were simultaneously measured by a high performance liquid chromatography (HPLC). The plasma was acidified with 0.2 M HCl and 0.2 M orthophosphoric acid, and mixed with acetonitrile. ASA and SA extracted with acetonitrile were analyzed by the HPLC equipped with reversed phase Novapak C18 column (4 ㎛ silica, 150×4 mm) and UV detector(237 nm). The mobile phase consisted of 740 ㎖ water, 900 ㎕ orthophosphoric acid (85%) and 180 ㎖ acetonitrile. The retention times of ASA, SA and 2-methylbenzoic acid(MBA) were 4.8 min, 8.4 min and 11.5 min, respectively. The limit of quantification was 0.01 ㎍/㎖ for SA and 0.05 ㎍/㎖ for ASA. The mean recovery from eel plasma was 70.8～99.6% for ASA and 95.2～100.3% for SA. This HPLC method was applied to analyze ASA and SA of eel plasma after either dipping in a concentration of 20 ppm or feeding the feed supplemented with 50 ㎎/kg BW. Only SA was detected in eel plasma after the administration of ASA by dipping or oral routes because the drug was quickly decomposed into SA in eel plasma. The amount of SA in eel plasma reached the highest value at 3hr in dipping and 7 days in oral administration. When the ASA-administrated eel were kept in ASA free aquaria, 0.02-0.03 ㎍/㎖ of SA were detected 48 hr after the administration in both routes.