• Journal of Environmental Science International
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• v.17 no.4
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• pp.439-449
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• 2008

Algicidal bacterium was isolated from sea water during the declining period of Cochlodinium polykrikoides blooms and this bacterium had a significant algicidal activity against C. polykrikoides. In this study, algicidal bacterium was identified on the basis of biochemical and chemotaxonomic characteristics, and analysis of 16S rDNA sequences. The algicidal bacterium showed 98.6% homology with Micrococcus luteus ATCC $381^T$. Therefore, this bacterium was designated Micrococcus luteus SY-13. The optimal culture conditions of the algicidal bacterium was $25^{\circ}C$, initial pH 8.0, and 3.0% NaCl concentration. M. luteus SY-13 is assumed to produce secondary metabolites which have algicidal activity. When 10% culture filtrate of this strain was applied to C. polykrikoides ($1.0\;{\times}\;10^4\;cells/ml$) cultures, over 98% of C, polykrikoides cells were destroyed within 6 hours. The culture filtrate of M. luteus SY-13 exhibited similar algicidal activity after heat-treatment at $121^{\circ}C$ for 15 min. While algicidal activity remained in filtrates with pH adjusted to 8.0, loss of algicidal activity occurred when the pHs of filtrates were adjusted to over 9.0 or heat-treated at $121{\times}180^{\circ}C$ for 1 hour. M. luteus SY-13 showed significant algicidal activities against C. polykrikoides (98.9%) and a wide algicidal range against various harmful algal bloom (HAB) species. However, there was no algicidal effect on diatom and marine livefood organisms except Isocrysis galbana. These results suggest that M. luteus SY-13 could be a candidate for use in the control of HABs.

• Journal of the Korean Recycled Construction Resources Institute
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• v.4 no.3
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• pp.235-242
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• 2016

As for a possibility of using high volume of recycled aggregate in concrete mixture, recycled fine one which is known to be worse in quality and hard to control was selected and investigated in terms of performance of mortar as the replacement ratio to natural fine aggregate was changed. As a result of test, it is found that grade of recycled fine aggregate was beyond standard one and fineness modulus of that itself was increased in compare to natural one. In case of making mortar with recycled fine aggregate, disadvantageous results such as less fluidity and air content including the increase of dry shrinkage were shown but strengths of mortar were comparable to the one making with natural aggregate, which means that planned strength of common concrete structure can be achieved by controlling W/C and the amount of chemical admixture, and also that large amount of recycled fine aggregate is applicable to the precast concrete products generally free to the amount of water.

• The Journal of Advanced Prosthodontics
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• v.4 no.2
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• pp.84-88
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• 2012

PURPOSE. To evaluate the efficacy of sodium hypochlorite (1 : 10) and iodophor disinfectants on alginate impressions along with their effect on the survived bacterium count on the gypsum cast. MATERIALS AND METHODS. Four alginate impression on each dentate patients were made, of which Group I were not washed or disinfected, Group II impressions were merely washed with water, Group III were disinfected by spraying with sodium hypochlorite (1 : 10), Group IV were disinfected with iodophor (1 : 213). Gypsum cast (type III) were made from all the impression. Impressions and gypsum cast were swabbed in mid palatal region for bacterial culture. Bacterial colony counting done after 3 days of incubation at $37^{\circ}C$ in blood agar media. The data obtained was analyzed by one way ANOVA test at a significant difference level of 0.05. RESULTS. Group I and Group II showed significantly more bacteria compared to Group III and Group IV. Bacterial colonies on the alginate impression and gypsum cast in group disinfected with Sodium hypochlorite (1 : 10) were 0.18, 0.82 respectively compared to group treated with iodophor (1 : 213). There was an increase in bacterial count on dental cast compared to source alginate impressions. CONCLUSION. Sodium hypochlorite (1 : 10) was found to be better disinfectant for alginate impression. There was an indication of increase in number of bacteria from alginate impression to making of dental cast. Additional gypsum cast disinfectant procedures need to be encouraged to completely eliminate cross infection to dental laboratory.

• Journal of Technologic Dentistry
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• v.35 no.1
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• pp.57-64
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• 2013

Purpose: Waste parts of zirconia blocks and powders were remained after CAD/CAM process. In order to make these residual zirconia fit for practical use, zirconia single cores were produced by drain casting process. Methods: Remained zirconia blocks were reduced to powders with zirconia mortar, and screened with 180 mesh sieve. Zirconia slip was prepared from waste parts of zirconia by ball milling. Plaster molds for forming cores by slip casting were also prepared. Formed cores were removed from mold after partial drying. Dried cores were biscuit fired at $1,100^{\circ}C$ for 1hour. Biscuit fired cores were treated with tools to control the fitness and thickness. Finished cores were $2^{nd}$ fired at $1,500^{\circ}C$ for 1hour. Microstructure of cross section of core was observed by SEM. Results: When mill pot was filled with 100g of zirconia and alumina mixed powder, 300g of zirconia ball, and 180g of distilled water, the optimum slip for drain casting was obtained. Gypsum plaster for ceramic forming was more suitable then yellow stone plaster for casting process. SEM photograph showed the microstructure of fully dense with uniform grain size of zirconia and well dispersed alumina grains into the zirconia matrix. Conclusion: Zirconia single cores were produced by drain casting process. Drain casting is useful process to make these residual zirconia fit for practical use. Further study will be focused on the preparation of the bridge type cores by casting.

• Journal of Environmental Health Sciences
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• v.34 no.1
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• pp.55-61
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• 2008

This study was carried out to investigate the antioxidative and antitumor activities of Saussurea lappa for the purpose of developing a functional food. The methanol extract of Saussurea lappa was fractionated with five solvents (hexane, chloroform, EtOAc, BuOH, water) and examined for antioxidative activities and xanthine oxidase inhibitory activity in addition to growth inhibitory activity of human cancer cell (HT-29, SNU-1, HeLa). Total phenol compound contents were higher in EtOAC fraction than other fractions. Also, electron donating ability was over 90% at $500{\mu}g/ml$ (93.1 %) and $1000{\mu}g/ml$ (94.3%). The hexane fraction revealed stronger nitrite scavenging ability than the positive control (ascorbic acid) and its abilities were 22.4% and 42.8% at $500{\mu}g/ml$ and $1000{\mu}g/ml$, respectively. Xanthine oxidase inhibitory activity had similar tendency to electron donating ability. The EtOAc fraction showed high inhibition to xanthien oxidase activities at $500{\mu}g/ml$(81.9%) and $1000{\mu}g/ml$(90.4%). In the antitumor activity test, the hexane fraction exhibited potent growth inhibition activity against HT-29, SNU-1 and HeLa cells. Therefore, we believe that Saussurea lappa can be developed into a functional food with antioxidant activity. Additional studies are required for the hexane and chloroform fractions of Saussurea lappa to develop them into therapeutic supplements for stomach cancer, colon cancer, and cervical cancer.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.3
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• pp.405-410
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• 2015

The objective of this study was to evaluate the effects of sequential applications of ${\varepsilon}$-polylysine (EPL) or lauramide arginine ethyl ester (LAE) sprays followed by an acidic calcium sulfate (ACS) spray on inoculated chicken carcasses to reduce Salmonella (Salmonella enterica serovars including Salmonella typhimurium and Salmonella enteritidis) contamination during 6 days of storage ($4.4^{\circ}C$). Secondly, reductions of the resident microflora were studied on uninoculated chicken carcasses following the sequential application of the treatments, chilling and 10 days of storage at $4.4^{\circ}C$. The treatment of Salmonella inoculated carcasses with 300 mg/L EPL followed by 30% ACS (EPL300-ACS30) sprays reduced Salmonella counts initially by 1.5 log cfu/mL and then by 1.2 log cfu/mL (p<0.05) following 6 days of storage at $4.4^{\circ}C$. Likewise, 200 mg/L LAE followed by 30% ACS (LAE200-ACS30) treatment reduced initial Salmonella counts on poultry carcasses by 1.8, 1.4 and 1.8 log cfu/mL (p<0.05), respectively, after 0, 3, and 6 days storage. Immediately after the treatments, EPL300-ACS30 and LAE200-ACS30 both reduced Escherichia coli counts significantly by 2.6 and 2.9 log cfu/mL, respectively. EPL300-ACS30 and LAE200-ASC30 were effective in lowering psychrotroph counts by 1 log cfu/mL on day 10 when compared to the control and distilled water treatments. This study demonstrated that EPL300-ACS30 and LAE200-ACS30 were effective in reducing Salmonella on inoculated chicken carcasses both after treatment and during the storage at $4.4^{\circ}C$ for up to 6 days. In addition, reductions in psychrotroph counts indicated that these treatments might have the potential to increase the shelf-life of poultry carcasses.

• Journal of Aquaculture
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• v.9 no.3
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• pp.279-285
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• 1996

The effects of various concentrations of $17{\beta}-methyltestosterone$ (MT) or $17{\beta}-estradiol\;(E_2)$ on sex reversal, survival and growth rates of olive flounder, Paralichthys olivaceus were investigated. MT and $E_2$ were treated to juvenile flounder for 60 days from 30 (total length : 14.5 mm) to 90 (TL : $10.41\~11.17$ mm) days after hatching. Fish were treated with 0, 1, 10 and 100 ppb of MT or $E_2$ in the rearing water for 2 hours per day. At the time of 200 days after hatching, fish were sampled to examine sex ratio. One hundred pub of MT produced $100\%$ male, however 1 and 10 ppm MT produced 72.5 and $87.2\%$ males. One, 10 and 100 ppb of $E_2$ produced 75.5, 91.9 and $97.2\%$ females, respectively. Survival and growth rates of each experimental group at the end treatment were not significantly different from those of the control (P>0.05).

• Korean Journal of Construction Engineering and Management
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• v.7 no.3 s.31
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• pp.149-158
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• 2006

For construction projects, it is required to estimate the Environmental Management Cost(EMC) for pollution prevention, using the calculation standards prescribed in the Construction Technology Management Law(CTML). However, the EMC standards are difficult to utilize without definite site information. Therefore, it is needed a new calculation method reflecting project site information. According to the definition of EMC in the CTML, this study identifies the EMC items and classifies them into the pollution types such as air, water, noise, waste and others. With this EMC classification, the stud:』 analyzes using the SPSS the EMC for the 78 new multi-housing projects, which were executed during the past three years, 2000-2002. And then an EMC estimating matrix is developed with simple information such as site location and size of area from historical data. The proposed matrix can be effectively used to check and control budgeting and estimating the EMC of the multi-housing projects. In addition, the proposed EMC matrix are validated through a case study.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.4
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• pp.931-940
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• 2015

Roller Compacted Concrete Pavement (RCCP), is a type of pavement using compaction roller and asphalt finisher on concrete mixture that contains low amount of water. RCCP strength and durability are greatly affected by compaction level. Quality control is performed by ensuring the degree of compaction at site based on dry density. In the field, Modified Proctor Test is used in order to obtain optimum dry density. However, there is no clear compaction curve analysis criteria of Modified Proctor Test for RCCP. In this study, compaction curve built by three samples of Modified Proctor Test was produced and it was used to compare with compaction curve contented lower number of samples (one and two samples) in order to analyze their reliability. Thus, a conclusion was drawn from the results; by comparing to the result from Modified Proctor Test of three samples, the use of two samples represented result with only 0.5% of error which means the reliability is 99.5%.

• Korean Journal of Community Nutrition
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• v.22 no.3
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• pp.228-237
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• 2017

Objectives: The purpose of this study was to investigate the smoking status among adult workers, and current status of sugar intake. Methods: The survey included 500 men working in Gyeonggi-do from October to November in 2016. Questionnaire items covered their age, working status, smoking status, eating habits, eating behaviors, snack consumption status, habits and behaviors related to sugar intake. All data were analyzed by SPSS program (Ver. 23) and descriptive statistics was performed; a t-test, ${\chi}^2$ test, One-way ANOVA and Scheffe test were used for post-hoc test. Results: The study results showed that eating habits and behaviors of non-smokers were better than those of smokers. The frequency of daily snack consumption was the highest in smokers compared to and non-smokers. The smokers' favorite taste after smoking was 'Sweet'. The average score of sugar-related nutrition knowledge was higher in non-smokers compared to smokers. Non-smokers had better recognition of 'sugar reduction', and smokers were more likely to eat sweet foods, respectively. On the other hand, non-smokers could observe that they were trying to control themselves for health reasons. According to the results of the study, non-smokers showed better eating habits and dietary habits and consumed less sugar. Also, it was found that non-smokers tried to drink more water than beverages and refrain from eating sweets to reduce their sugar intake. Further, the most of the bread, coffee and beverages were also consumed at a lower frequency by non-smokers compared to smokers. Conclusions: This study results showed that smoking and sugar consumption were closely related. Therefore, adult workers should actively promote and learn so that they can maintain healthy and suitable dietary habits through reduction of sugar consumption.