• Title/Summary/Keyword: water control

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Simultaneous Determination of Hesperidin and Glycyrrhizin in Pyungwi-san by HPLC/DAD (HPLC-DAD를 이용한 평위산 중의 Hesperidin 및 Glycyrrhizin의 동시분석법 확립)

  • Lee, Mi-Kyeong;Choe, Ok-Gyeong;Park, Jin-Ho;Cho, Jung-Hee;Kim, Do-Hoon;Baek, Ju-Hyun;Kim, Hyo-Jin;Lee, Ki-Yong;Kim, Sang-Du;Kim, Young-Choong;Sung, Sang-Hyun
    • Korean Journal of Pharmacognosy
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    • v.39 no.3
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    • pp.199-202
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    • 2008
  • A high performance liquid chromatographic (HPLC) method for the simultaneous determination of hesperidin and glycyrrhizin was established for the quality control of traditional herbal medicinal preparation, Pyungwi-san (PWS). Separation and quantification were successfully achieved with a Waters XTerra RP18 column ($5{\mu}m$, 4.6 mm I.D. ${\times}$ 150 mm) by gradient elution of a mixture of acetonitrile and water containing 0.03% phosphoric acid (pH 2.03) at a flow rate of 1.0 ml/min. The diode-array UV/vis detector (DAD) was used for the detection and the wavelength for quantification was set at 230 nm. The presence of hesperidin and glycyrrhizin in this extract was ascertained by retention time, spiking with each authentic standard and UV spectrum. All four compounds showed good linearity $(r^2>0.995)$ in a relatively wide concentration ranges. The R.S.D. for intra-day and inter-day precision was less than 7.0% and the limits of detection (LOD) were less than 60 ng. The mean recovery of each compound was 99.0-105.6% with R.S.D. values less than 4.0%. This method was successfully applied to the determination of contents of hesperidin and glycyrrhizin in three commercial products of PWS. These results suggest that the developed HPLC method is simple, effective and could be readily utilized as a quality control method for commercial PWS products.

Effect of Dietary Herb Mix on the Physicochemical Quality of Cooked Chicken Egg during Refrigerated Storage (복합 한약재 급여가 냉장 저장 중 삶은 계란의 물리화학적 품질에 미치는 영향)

  • Liu, X.D.;Jang, A.;Shin, M.H.;Lee, B.D.;Lee, S.K.;Kim, E.M.;Jo, C.
    • Korean Journal of Poultry Science
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    • v.35 no.1
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    • pp.1-7
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    • 2008
  • Three dietary herbs, Coptis japonica Makino (48.5%), Lonicera japonica Thunb (48.5%), and Morus alba L. (3%) were mixed and used as additives in hen's feed. One hundred-eight, 28-wk-old Lohmann Brown hens were assigned randomly with three diet treatments (0, 0.3, and 1% of herb mix). Hens were fed for 6 wks to investigate the effect of herb mix on the physicochemical quality of eggs during storage at $4^{\circ}C$ for 0, 3, 7, and 14 days. Proximate composition including the content of water, protein, fat, and ash had no difference among 3 diet treatments. Objective color of cooked whole egg from hens fed herb mix did not show any effect compared with control except for the color $a^*$- and $b^*$-values at day 3. However, color $a^*$- and $b^*$-values increased during storage regardless of treatment. From the texture analysis, eggs from hens fed with the herb mix were not different compared with control remove for springness at day 14. However, it was observed that springness and gumminess were decreased as storage period increased. Therefore, a dietary supplementation of herb mix to hen may not affect on proximate composition, color and texture of eggs.

Effect of Dietary Supplemention with Probiotics, Illite, Active Carbon and Hardwood Vinegar on the Performance and Carcass Characteristics of Broiler (사료내 생균제, 일라이트, 활성탄 및 목초액의 첨가가 육계의 성장 능력 및 도체 특성에 미치는 영향)

  • Kim, Y.J.
    • Korean Journal of Poultry Science
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    • v.34 no.3
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    • pp.165-172
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    • 2007
  • We investigated the effects of dietary supplements of probiotics, illite, active carbon and hardwood vinegar on growth performance, feed intake, and pH, shear force, sensory evaluation, meat color and fatty acid composition of meat in broilers. Two hundred broilers were fed diets for five weeks containing 0.2% of probiotics (T1), and 1% of Illite (T2), 1% active carbon (T3), or 1% hardwood vinegar (T4). Body weight gain was higher in T1 and T4 groups fed the starter diet but was the lowest in C and T4 for finishing period (P<0.05). Feed efficiency was not significantly different. In proximate composition, crude fat content of chicken meat were decreased lower in all treatment groups than control, but moisture, crude protein and crude ash were not significantly different. Cooking loss was decreased in T3 and T4 and WHC (water holing capacity) was increased in T3 and T4 groups compared to the other groups. In sensory evaluation, T4 tended to improve the hardness. Redness $(a^*)$ and yellowness $(b^*)$ were no difference between the all treatment groups, lightness $(L^*)$ were higher in T1, T2, T3 and T4 groups than control group (P<0.05). Stearic acid content was lower in T1, T2, T3 and T4 groups, but oleic acid contents were higher in T1, T2, T3 and T4 groups (P<0.05). These results showed that supplementing broiler diets with 1.0% hardwood vinegar may noticeably improve the meat quality of broiler.

Improvement of Takju Quality by a Ripening-Fermentation Process Using Honey and Extension of Shelf Life by Control of Takju Mash Sediment (벌꿀을 이용한 고액분리 숙성 탁주의 주질 향상과 고형분의 조절에 따른 품질유지기간 증대)

  • Jung, Seung-Jin;Shin, Tai-Sun;Kim, Jin-Man
    • Journal of Life Science
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    • v.22 no.1
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    • pp.80-86
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    • 2012
  • This study was conducted to improve quality of takju where natural honey is used to control the ripening fermentation and the amount of sediment derived from takju mash. A koji was prepared using rice starch and Aspergillus awamori var. kawachii. Takju mash was prepared by alcohol fermentation with Saccharomyces cerevisiae and a 3-step addition of steamed rice. The clean part of the mash (CPM) was separated from the sediment at $5^{\circ}C$ and 5% (w/v) of natural honey was added and then ripened for 23 days at 5, 10, or $15^{\circ}C$. Temperature, pH, acidity, and total sugar content showed no significant differences, but a 0.2 percent reduction in alcohol content occurred during storage. However, CPM ripened with honey had a comparatively higher score on sensory evaluation than did immature CPM with added honey added. Takju with 8% alcohol content was prepared by mixing the water from the mixed CPM ripened with honey together with 100, 50, and 25% of the frozen sediment. Several quality characteristics of the takju were checked over 37 days of fermentation at $10^{\circ}C$. The pH was sustained between 4.1 and 4.3, and changes in the number of viable yeast cells, acidity, total sugar amounts, and alcohol content showed similar patterns but differences in scale. Smaller amounts of sediment affected the stability of the takju. Mixing the CPM ripened with natural honey at low temperature moderately reduced the amount of sediment in the mash and resulted in a highly flavorful takju with an extended shelf life.

Effects of the Prunus persica Batsch var. davidiana Max. Extract on the Blood Glucose and Serum Lipid Components in Streptozotocin-Induced Diabetic Rats (돌복숭아(Prunus persica Batsch var. davidiana Max.) 추출액이 Streptozotocin 유발 당뇨성 흰쥐의 혈당 및 지질 성분에 미치는 영향)

  • Kim Han-Soo
    • The Korean Journal of Food And Nutrition
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    • v.17 no.3
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    • pp.337-345
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    • 2004
  • The purpose of this study was designed to observe the effects of the feeding Prunus persica Batsch var. davidiana Max. extract on the improvement of the blood glucose, lipid compositions in the serum of streptozotocin(STZ)-induced diabetic rats fed the experimental diets for 5 weeks. Concentrations of blood glucose, total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, free-cholesterol, cholesteryl ester, triglyceride(TG) and phospholipid (PL) in serum were significantly higher in the STZ (55 mg/kg B.W.)-induced diabetic group (group 2) and STZ(I.P.)+Prunus persica 5.0 g% extract group(group 3) than those in the control group (group 1, basal diet + water). But the concentrations of blood glucose, total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, free-cholesterol, cholesteryl ester, TG and PL in serum were remakably lower in the group 3 than those in the group 2. In the ratio of HDL-cholesterol concentration to total cholesterol and HDL-cholesterol concentration, Prunus persica 5.0 g% extract administration group(group 3) were higher percentage than in the group 2. The activities of aspartate aminotransferase(AST), alanine aminotransferase(ALT), lactate dehydrogenase (LDH) and alkaline phosphatase(ALP) in serum were rather lower in the Prunus persica 5.0 g% extract administration group(group 3) than in the STZ- induced diabetic group (group 2). From the above results, it was suggested that the Prunus persica Batsch var. davidiana Max. were effective on the improvement of the blood glucose, lipid compositions in serum of STZ-induced diabetic rats. Moreover, in Prunus persica Batsch var. davidiana Max. was effective therapeutic regimen for the control of metabolic derangements in adult disease.

Effects of Immersion Period after Tumbling Processing on the Quality Properties of Boiled Pork Loin with Soy Sauce (텀블링 공정 후 침지기간이 간장첨가 삶은 돈육의 품질 특성에 미치는 영향)

  • Choi, Yun-Sang;Jeong, Jong-Youn;Choi, Ji-Hun;Lee, Mi-Ae;Lee, Eui-Soo;Kim, Hack-Youn;Han, Doo-Jeong;Kim, Jin-Man;Kim, Cheon-Jei
    • Korean journal of food and cookery science
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    • v.22 no.3 s.93
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    • pp.379-385
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    • 2006
  • The purpose of this study was to investigate the effects of immersion period after tumbling on the physicochemical properties of boiled pork loin with soy sauce. After the samples were tumbled for 30 min with soy sauce (1.5% salt concentration), they were immersed for 1, 5 or 10 days. Boiled pork loin was cooked at an internal temperature to $75^{\circ}C$. Cooking yields of boiled pork loin were significantly increased with increasing immersion period. The reduction in diameter and thickness for pork loin were significantly decreased with increasing immersion period, but moisture content and water holding capacity were increased. Shear force in the immersed samples after tumbling was significantly lower than in the control. In sensory evaluation color, flavor, texture, and juiciness of boiled pork loin immersed after tumbling should higher ratings than those of control.

Agronomic Characteristics of Waxy Hybrid Corn on the paddy soil conditions (논 토양조건에 따른 찰옥수수 교잡종의 작물학적 특성)

  • Ji, Hee Chung;Kim, Choong-Soo;Hong, Bom-Young;Lee, Hee-Bong
    • Korean Journal of Agricultural Science
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    • v.33 no.2
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    • pp.123-127
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    • 2006
  • This study was carried out to compare growth and yield performance for soil conditions at the Maize Genetics and Breeding Lab. in Chungnam National University. The waxy hybrid corn in the drained soil of paddy field showed higher fresh yield per 10a and 100-kernel weight per plant than control. The average dry yield of drained and drainless soil were 804kg/10a and 498kg/10a compared with a control (751kg/10a), respectively. The waxy hybrid corn in the drained soil also had better good stand and stay green characteristics than drainless soil condition, and showed moderately resistance to water stress and weed. Vegetational feature of weeds was characterized as the prevalence of Acalypha auatralis, Echinochloa crus-galli, Persicaria hydrpopiper in drained paddy soil and Persicaria hydrpopiper, Acalypha auatralis, Echinochloa crus-galli in drainless paddy soil, and Echinochloa crus-galli, Acalypha auatralis in upland soil.

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Effect of ultrasonication, salt solution and liquid smoke treatment on germination of Setaria italica seeds

  • Kim, Young Ae;Kim, Min Geun;Oh, Ju-Sung;Kim, Du Hyun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.215-215
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    • 2017
  • The preference for domestic cereal crop of Setaria Italica has been increased with the recent interest in healthy foods. However, the productivity of Korean domestic produce, which has been decreasing due to the lack of cultivation technology and the low rate of mechanization during cultivation. Increase of germination ability will have a positive effect on the cultivation by reducing the labor of the manpower consuming and weeding work. Therefore, red light, ultrasonication, liquid priming and liquid smoke treatment that are effective for the germination of the seeds evaluated. The seeds of 1.4mm or more were used for the experiment. The priming solution used in the experiment was 1% $KH_2PO_4$ (74mM). During the priming, the light treated seeds at 2000 lux for 15, 30, 60 and 120 minutes. Ultrasonicationd treatment was performed for 5, 10, and 20 minutes at exposures of 60%, 80%, and 100% of ultrasound up to 21.6 kHz during priming. Light or ultrasound treated seeds transferred to priming treatment at $15^{\circ}C$ for 24 hours. The treatment of the liquid smoke was divided into the treatment of the liquid smoke alone and the treatment of the liquid smoke with the priming. The liquid smoke alone was diluted with distilled water without priming solution and the treatment of the liquid smoke was diluted with the salt priming solution. Both treatments were performed at 0.0%, 0.5%, 1.0%, 5.0%, and 10.0% of the liquid smoke (pH7) concentration at $15^{\circ}C$ for 6 hours. After each treatment, the seeds were dried to moisture content ranged 5-8% at $25^{\circ}C$ for 24 hours. All treatments showed better results than the non-treated control. Light treatment for 120 minutes improved for germination percentage (GP), Germination uniformity (GU) and heath seed percentage (HS). Ultrasonication treatment was most effective when treated with ultrasound at 21.6 kHz for 5 minutes in all germination characteristics. Ten % of the liquid smoke increase in 92% GP, 1.8 days MGT, $54%{\cdot}day^{-1}GR$, 0.76 GU and 88% HS comparing to non-treated control (72% GP, 2.3 days MGT, $45%{\cdot}day^{-1}GR$, 1.48 GU, and 63 % HS). This study showed that it is possible to obtain high germination by adding liquid smoke treatment to the seeds supplied to the farmers. The efficacy of light, ultrasonication, inorganic salt priming, and liquid smoke treatment on the seeds found in the experiment will be a positive alternative to labor force problems in the cultivation by improving germination.

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Effect of Lighting Regimes on Performance and Meat Quality of Broilers (점등체계가 육계의 생산성과 육질특성에 미치는 영향)

  • Na, Jae Cheon;Park, Sung Bok;Kang, Hwan Ku;Kim, Dong Wook;Kim, Min Ji;Bang, Han Tae;Chae, Hyun-Seok;Choi, Hee Chul;Suh, Ok Suk;Hong, Eui Chul
    • Journal of Animal Environmental Science
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    • v.18 no.3
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    • pp.173-182
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    • 2012
  • The study was carried out to investigate the effect of lighting regimes on performance and meat quality of broiler. A total of 912 chicks was divided into 2 groups with male (3 replications/group, 34 heads/replication) and female (3 replications/group, 42 heads/replication). Treatments were control (C, natural lighting), T1 treatment (24L), and T2 treatment (1~2d, 24L; 2~4d, 23L+1D; 4~16d, 16L+8D; 16~21d, 18L+6D; 21~42d, 23L+1D) by lighting regimes. There was no significantly different on body weight, body weight gain, feed intake, and feed conversion ratio of male and female of broilers among treatments. There was no significantly different on cooking loss and water hold capacity of all broiler's meats among treatments. Share force of lighting regimes was higher compared to control (P<0.05). There was no significantly different on common ingredients (moisture, crude protein, crude fat, and ash) of broiler's meat. Finally, lighting regimes may decrease the stress as well as improve the growth performance and meat quality of broilers

Effects of Dandelion (Taraxacum coreanum) Extracts on the Mouse Liver with Acute Toxicated by Mercury Chloride (흰민들레(Taraxacum coreanum)추출물이 급성 수은 중독된 생쥐의 간에 미치는 효과)

  • Cheong, Min-Ju;Yoon, Jung-Sik;Huh, Jin;Roh, Young-Bok;Choi, Young-Bok;Kim, Jong-Se;Lee, Hyun-Hwa
    • Applied Microscopy
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    • v.38 no.1
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    • pp.1-10
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    • 2008
  • Dandelion has been frequently used as a remedy for women's disease, inflammatory diseases and disorders of the liver and gallbladder. Dandelion extracts water extract, an herbal medication, may have an effect on the activity of hepatic antioxidant enzymes in diabetic rat. This study aims demonstrate the effect of dandelion extracts, one of the natural chelator, on the biochemical and enzyme activity changes in the mouse liver caused by $HgCl_2$. Mice approximately 30 gm in weight were grouped into the control, mercury chloride-treated, and the dandelion extracts-treated after mercury chloride groups. $HgCl_2$ (5 mg/kg) and dandelion extracts (3 g/kg) were delivered orally. Serum AST and ALT were measured, enzyme activity of liver were examined by spectrophotometer and ultrastructural alteration of liver were examined by light and electron microscopy. Dandelion extracts were decreased the increase of serum AST and ALT level induced by mercury. The catalase activity was decreased in the dandelion extracts group. The activity of SOD was dereased, but did not show significant differences. Mercury chloride-treated hepatic cell were irregular nucleus, enlarged and reduced number of mitochodria, enlarged rough endoplasmic reticulum, loss of ribosomes. Cells treated with dandelion extracts were similar to those of the control group. In conclusion, dandelion extracts may protect the mercury-induced toxicity on Liver.