• Biomolecules & Therapeutics
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• v.5 no.2
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• pp.165-173
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• 1997

This study was conducted to investigate the effect of DA-9601, an extract of Artemisiae Herba, which is known to possess mucoprotective action either by free radical scavenging effect or increase of mucus secretion, against animal models of inflammatory bowel disease (IBD) induced by trinirobenzene sulfonic acid (TNBS) or other noxious agents. Experimental colitis was induced by intracolonic administration of TNBS in 50% ethanol, or 1 ml of 7% acetic acid solution (AA), by subcutaneous injection of indomethacin (INDO) in rats, or by supplementing drinking water with 5% dextran sodium sulfate (DSS) in albino mice. DA-9601 was treated orally for 4 to 7 days. Animals were euthanized 1 day after the last treatment for morphological and biochemical analysises. All the noxious agents including TNBS, AA, INDO and DSS elicited severe colitis. The animals treated with DA-9601 showed a consistent, dose-related reduction in the severity of colitis, grossly and histologically. The reduction was significant (p<0.05) after administration of DA-9601 at dose range of 10 mg/kg or above. In TNBS-induced colitis, the rats receiving DA-9601 showed significantly decreased mucosal myeloperoxidase (MPO) and thiobarbituric acid-reactive substances (TBA-RS), when compared to control and mesalazine groups. Mucosal proinflammatory cytokine levels were also decreased after DA-9601 treatment. In conclusion, DA-9601 ameliorated macroscopic and histologic scores in experimental colitis either through decreasing oxidative stress or by attenuating cytokines involved in inflammation. DA-9601 could be a promising drug for the therapy of IBD.

• Biomolecules & Therapeutics
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• v.13 no.3
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• pp.165-173
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• 2005

The main aim of this study was to investigate stress related activities of ginsenosides and their action mechanism. Control group and ginsenoside supplemented groups were exposed to stress while no-stress group was not done. Animals of each group (n=$8\~10$) were orally administerd 100 mg red ginseng extract (R-G), or 10 mg ginsenosides/kg body weight once a day. Animals were given materials for 5 days without stress, and then were given supplements for 5 days with restraint and electroshock stress. Mice were given materials for 5 days for experiments on anti-fatigue effect. After loading final stress, stress-related behavioral changes of experimental animals were examined and plasma corticosterone levels were measured. R-G and ginsenoside $Rb_{1}$ supplementation partially blocked the stress effects on locomotion and elevated plus-maze test in rats and mice. They also partially blocked stress induced behavioral changes such as freezing, smelling, face-washing, rearing behavior in rats. R-G and $Rb_{1}$ decrease adrenal gland size and plasma corticosterone level, which were increased by stress in rats. R-G increased enduring time on the Rota rod, cold water and horizontal wire, but $Rb_{1}$ didn't. Effects of $Rb_{1}$ on plusmaze test were inhibited by administration of flumazenil. These results suggest that $Rb_{1}$ is the main antistress principle in ginseng and it's effect is modulated by GABAnergic nervous system.

• Journal of Soil and Groundwater Environment
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• v.15 no.1
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• pp.57-65
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• 2010

Currently, the pursuit of industrialization for rapid economic growth has led to serious environmental damage and related problems because of comtamination with chemicals. Over time, this contaminaion is getting worse and worse. Most developing countries have tried to pursue their economic development and growth, however without preparation for the control and disposal of hazardous wastes. Permitting hazardous waste to be abandoned and disposed in appropriately causes soil pollution, groundwater contamination, and surface water contamination, which are all extremely serious. In particular, when hazardous wastes which include toxic chemicals are distributed around the living environment even in small amounts, concentration can occur in the food chain through ecological systems, which can therefore affect human. Chromium(VI) has contaminated soil in China. We experimented to test the immobilization, chemical reduction, and soil washing methods in order to make our experiment efficient in the purification of soil contaminated with Chromium(VI), and to prove the theoretical models by experiments testing the field applications. The field experiment showed that result of experiment satisfied with the standards of China. The result of multi-stage continuous remediation reduce the Chromium(VI) concentration to 11.95 mg/kg and 14.83 mg/kg that were equivalent to 98% and 97% removal rates. This result implied we could apply multi-stage process of Chromium(VI) contaminated soil to meet the regulatory limit of Chromium(VI).

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.5
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• pp.558-564
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• 2010

Cleaning up the landfill soil by phytoremediation in association with biomass production and utilization of biosolid as a soil amendment will be an attractive green technology. In order to examine this integrated green technology, in the current study of pot trial, heavy metal removal rate and biomass production were determined following cultivation of three different plant species in the landfill soil incorporated with biosolid at two different levels (25 ton $ha^{-1}$ and 50 ton $ha^{-1}$). Among the three plant species including Indian mustard (Brassica juncea), giant sunflower (Helianthus giganteus. L), and giant cane (Arundo donax. L), sunflower appeared to produce the largest biomass yield (19.2 ton $ha^{-1}$) and the produced amounts were magnificently increased with biosolid treatment compared to the control (no biosoild treatment). The increased production associated with biosolid treatment was common for other plant species and this was attributed to the biosolid originated nutrients as well as the improved soil physical properties due to the organic matter from biosolid. The elevated heavy metals in soil which was originated from the incorporated biosolid were Cu and Zn. Based on the phytoavailable amount of heavy metals from biosolid, the removed amount by plant shoots were 95% and 165% for Cu and Zn, respectively, when sunflower was grown. This indicated that mitigation of heavy metal accumulation in soils achieved by the removal of metal through sunflower cultivation enables the successive treatment of biosolid to soils. Moreover, sunflower showed heavy metal stabilization ability in the rhizosphere resulting in alleviation of metal release to ground water.

• Korean Journal of Soil Science and Fertilizer
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• v.51 no.1
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• pp.1-7
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• 2018

The veterinary antibiotics treated to livestock have a potential risk to reach to soil and water environment, and eventually be taken up by plants. The objective of the study was to investigate the effect of zeolite and shell meal fertilizer amendment on antibiotic uptake by plant when veterinary antibiotics in chicken manure compost were applied to agricultural land. Model antibiotics used in the study were chlortetracycline (CTC), tylosin (TYL), and sulfamethazine (SMT). Chlortetracycline level in lettuce was decreased to less than $0.08ug\;kg^{-1}$ by application of zeolite as compared with about $0.26ug\;kg^{-1}$ for control without amendment on 33 days after transplanting. Tylosin was not detected for all the treatment. Sulfamethazine levels in lettuce ranged from 11 to $19{\mu}g\;kg^{-1}$ on a fresh weight basis and gradually decreased with time. Zeolite application decreased the SMT levels in lettuce by greater extent than shell meal fertilizer amendments. Results from the 61-d greenhouse experiment imply that application of zeolite at a rate of $1.5Mg\;ha^{-1}$ or shell meal fertilizer at a rate of $2.0Mg\;ha^{-1}$ can reduce CTC and SMT concentration in lettuce cultivated in soil fertilized with antibiotic-contaminated chicken manure compost.

• Korean Journal of Veterinary Research
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• v.34 no.3
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• pp.609-617
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• 1994

This study was performed for assessing carcinogenicity of Folpet using medium-term carcinogenicity bioassay. Sprague-Dawley rats aged six weeks divided into four grout's and were initially given an intraperitoneal injection of diethylnirosamine at 200mg/kg body weight. Two weeks later, group 1(negative control) was treated with basal diet. A Folpet was given per oral administration to group 2(100 ppm) and goup 3(1,000 ppm). Group 4 was fed on water containing 0.05% phenobarbital sodium as a promtor for six weeks. At three weeks after beginning of the experiment, partial hepatectomy was performed in all rats. The tumor-promoting effects were examined by the numbers and areas per $cm^2$ of induced glutathion S-tranferase placetal form(GST-P) positive foci in liver, and silver stained nucleolar organizer regions(AgNORs) which have recently introduced as one of the indicators for the cell proliferative activity. As the results, Folpet didn't have tumor-promoting effects on GST-P positive foci developement and AgNORs during promoting stage after initiation, whereas phenobarbital sodium treatment group showed promoting effect. It was concluded that Folpet didn't have promoting effect at 500, 1,000 ppm using this midium-term carcinogenicity bioassay model.

• Journal of Wetlands Research
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• v.15 no.4
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• pp.453-460
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• 2013

Rural area sewage distribution rate is 40 % less than urban area. Previous research results announced that one of the reason in water resource pollution was caused of low rural area sewage distribution rate. Analysis on operation result of 11 RCSTP in Bong-hwa area which is located at the upper region of An-dong Dam and Im-ha Dam. Based on operation result, an analysis on characteristics of wastewater in Bong-hwa and treatment efficiency of installed treatment process was conducted. The wastewater influent of Bong-hwa contained high organic matter and nutrient than those of urban area. Treatment process showed totally stable treatment efficiency. But, The treatment efficiency for nutrient showed high fluctuation. This result means which is need to operation condition control of nutrient treatment when operating RCSTP.

• Journal of Microbiology
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• v.40 no.1
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• pp.77-81
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• 2002

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pleiotropic hematspoietic growth factor involved in the development of myeloid cells from bone marrow, and an activator of mature myeloid cells functioning in a variety of antimicrobial and inflammatory responses. Recently, recombinant GM-CSF is increasingly under clinical study for treatment of various diseases including cancer, infectious diseases and hematopoietic diseases as well as for an immune response modulator, In this study, we constructed a recombinant human GM-CSF (rhGM-CSF) expression plasmid with a pelB leader sequence and His. Tag under T7 promoter control. The expression construct was shown to produce a recombinant protein of 20 kDa in the 8M urea preparation, indicating the rhGM-CSF may be expressed as an insoluble inclusion form. The 20 kDa recombinant protein in 8M urea was transformed into the water-so1ub1e form by dialysis against PBS buffer (phosphate buffered saline). The soluble rhGM-CSF protein was shown to stimulate colony formation and cell proliferation in vitro, indicating that the rhGM-CSF could be refolded into its native form to show colony stimulating activity.

• Journal of Energy Engineering
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• v.13 no.1
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• pp.82-91
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• 2004

In order to utilize inorganic components in coal and wastewater sludge as an environmentally stable material, slag-forming is considered as one of the suitable methods better than producing as an ash. Coal slag that was produced by gasification as well as the slag made from wastewater sludge by incineration or melting process have been analyzed with the viewpoint of recyclability. Slags produced by water quenching exhibited a cracked shape that has a size of few millimeters with sharp edges. Slags contain the unburned carbon content below 0.15％ and expose mostly amorphous structural characteristics. Analysis results in the extraction of heavy metal compounds demonstrate that both slags from coal and wastewater sludge could be utilized as a safe recycle material even with a Japanese environmental regulation that is ten times more stringent than the current Korean standard. Slags from coal and wastewater sludge show significant differences in contents of each heavy metal compound. Since the future trend of environmental regulation shifts to the control of total content for each heavy metal compound, proper mixing of slags that contain different heavy metal contents might be an option for manufacturing recycle materials.

• Journal of the Korea Furniture Society
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• v.22 no.1
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• pp.54-62
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• 2011

The metalized wood composites with natural grain of imported Juglans nigra, which was impregnated with low melting alloy were manufactured and evaluated in this study. And the proper manufacturing conditions was also investigated in this study. The low melting alloy with bismuth (Bi) and tin (Sn) which are harmless to humans, was applied to this new composites. The composites showed not only no defects of discoloration, delamination, swelling, and cracking, because of high dimensional stability and low thickness swelling, but also much improved performance such as high bending strength, high hardness, abrasion resistance, high thermal conductivity as floor materials. This study also suggested the proper impregnating condition, such as 10 minutes of the preliminary vacuum time, $186^{\circ}C$ of the heating temperature and 10 minutes of the maintaining pressure time at the pressure of $30kgf/cm^2$. This metalized wood composites showed 7 times higher density than control, great increase in bending strength from $131.8N/mm^2$ to $192.3N/mm^2$, and great increase in hardness from $18.2N/mm^2$ to $90.4N/mm^2$. The composites demonstrated not only high emissivity of 91%, high shilding effectiveness of 92.59∼99.99%, high fire resistance but also great decrease in abrasion depth, water absorption and thickness swelling.