• The Plant Pathology Journal
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• 제32권4호
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• pp.311-320
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• 2016

Xanthomonas axonopodis pv. glycines (Xag) is a necrotrophic bacterial pathogen of the soybean that causes bacterial pustules and is a nonhost pathogen of the chili pepper. In the current study, chili pepper fruit wound inoculated in planta with Xag 8ra formed necrotic lesions on the fruit surface and induced several structural and chemical barriers systemically in the fruit tissue. The initial defense response included programmed cell death of necrotizing and necrotized cells, which was characterized by nuclear DNA cleavage, as detected by TUNEL-confocal laser scanning microscopy (CLSM), and phosphatidylserine exposure on cell walls distal to the infection site, as detected by Annexin V FLUOS-CLSM. These two responses may facilitate cell killing and enhance transportation of cell wall materials used for cell wall thickening, respectively. The cells beneath the necrotic tissue were enlarged and divided to form periclinal cell walls, resulting in extensive formation of several parallel boundary layers at the later stages of infection, accompanying the deposition of wall fortification materials for strengthening structural defenses. These results suggest that nonhost resistance of chili pepper fruit against the nonhost necrotrophic pathogen Xag 8ra is activated systematically from the initial infection until termination of the infection cycle, resulting in complete inhibition of bacterial pathogenesis by utilizing organspecific in situ physiological events governed by the expression of genes in the plant fruit organ.

• Tuberculosis and Respiratory Diseases
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• 제47권1호
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• pp.66-76
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• 1999

연구배경: 비소세포성 폐암에서 장측 흉막 침범은 CT로 평가된 적이 드물고, CT상 기관지혈관속 비후는 병리적으로 확인되는 적이 드물다. 이들 침범양상이 국소적 재발과 원격 재발 그리고 생존에 영향을 미치는 요인을 분석하고자한다. 방법: 1986년에서 1995년까지 NSCLC로 근치적 수술을 시행 받은 434예 중에서 적어도 18개월에서 최장 103개월까지 추적 관찰하여 국소적 재발과 원격 전이 그리고 생존 여부를 확인한 81예를 대상으로 하였다. 장측 흉막 침범 여부 그리고 동측 폐엽 기관지혈관속 비후 여부에 따른 국소적 재발과 원격 전이 그리고 생존에 미치는 영향을 분석하였다. 장측 흉막 침범의 평가를 위해 고해상도 전산화 단충 촬영을 시행하고, 흉막이나 폐열에 근접한 종괴가 있었던 32예를 가지고 장측 흉막 침범 여부에 대한 고해상도 전산화단층촬영의 유용성을 알아보았다. 결과: CT상 종괴가 폐열을 건너간 경우에 장측 흉막 침범 진단에 대한 각각 높은 양성, 음성 예측도를 보였다(100%, 100%). 종괴와 면한 열이 종괴와 붙어 있거나 누르고 있는 경우 중에 불규칙한 변연을 보이는 2예에서는 모두 장측 흉막 침범을 보였다. 병리학적으로 확인된 장층 흉막 침범 여부는 국소적 재발에 유의한 차이가 있었고 (p<.05), 생존율에도 차이가 있었다(p<.05). CT상 동측 폐엽 기관지혈관속 비후 여부는 국소적 재발과 원격재발 모두 유의한 차이가 없었으나, 생존율에는 유의한 차이가 있었다(p<.05). 결론: 창측 흉막 침범 여부 그리고 CT상 동측 폐엽 기관지 혈관속 비후 여부는 재발와 생존에 영향을 미치는 요인으로 확인되었다. 따라서 장측 흉막 침범에 대한 CT의 분석과 기관지혈관속 비후의 병리학적 확인은 폐암환자의 예후 추정에 중요하다.

• Journal of Ginseng Research
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• 제13권1호
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• pp.71-78
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• 1989

인삼과실의 발달에 따른 과피의 구조적 변화를 광학 및 전자현미경으로 관찰하였다. 인삼과피는 크게 외과피, 중과피 및 내과피로 구분되었으며 외과피는 큐티클로 덮혀있는 단층표피와 하표피층으로 구성되었다. 외과피와 중과피층은 세포질의 대부분을 액포가 차지하고 있었고 세포의 배열이 불규칙적이었으며 분화가 진행될수록 세포벽의 용해가 일어나는 등 세포의 퇴화현상이 관찰되었다. 내과피 세포는 이차세포벽의 비후와 목질화 현상으로 다른 층과 쉽게 구별이 되었으며 이는 기계적인 보강조직으로의 분화라고 사료된다. Golgi comples와 조면소포체에서 생성되는 분비물질들을 세포벽에 축적되는데 이는 내과피가 종피로 분화될 때 종피의 주요 구성물질을 이루는 것으로 사료된다.

• 한국식물병리학회지
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• 제14권5호
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• pp.386-392
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• 1998

Reproduction of the soybean cyst nematode (SCN), Heterodera glycines Ichinohe on the susceptible soybean cultivar, Lee 74, was significantly reduced by pre-, post- and simultaneous treatments of acetylsalicylic acid (ASA, aspirin). The control efficiencies were 60%, 64% and 87% for pre-, post- and simultaneous treatments, respectively. ASA had no significant effect on the survival of 2nd stage juveniles and their penetration into the soybean root tissues, but significantly inhibited the early stage nematode growth in the roots. Syncytia were formed 2∼3 days after inoculation in the susceptible soybean without ASA treatment, characterized by dense cytoplasm and increased cellular organelles such as mitochondria and endoplasmic reticulum. The nematode stylet was penetrated into the syncytial cell, and feeding tube was formed at the nematode stylet was penetrated into the syncytial cell, and feeding tube was formed at the nematode stylet entry. However, in the ASA treatments, syncytium was not formed or degenerated, depending on the root tissues. In the pre-treatments of ASA, nematode stylets did not penetrate into cells, showing callose-like cell wall thickening formed at the nematode probing sites, or retracted from the infected cells. The stylet penetration sites of syncytial cells appeared to be sealed off with fibrillar materials. With post-treatment of ASA, syncytia formed by the nematode were degenerated, characterized by degradation of syncytial cytoplasm.

• Restorative Dentistry and Endodontics
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• 제25권4호
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• pp.491-493
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• 2000

A case of a botryoid odontogenic cyst of the globulomaxillary area between the right upper lateral incisor and the canine presenting as an apical periodontal cyst was reported. The cyst showed an unilocular radiolucency with a well delineated hyperostotic border. Histologic examination revealed multiple cysts lined by one or two-cell layers, some areas demonstrated a bud-like thickening of the epithelium. Clear cells were also conspicuous, but devoid of inflammatory reaction in the cystic wall. Some considerations regarding differential diagnosis, histogenetic and biologic behaviour of the lesion were discussed.

• Applied Microscopy
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• 제8권1호
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• pp.1-8
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• 1978

The aim of this research was to discover the action mechanism of various antituberculosis agents (isoniazid, paraaminosalicylic acid and streptomycin) which act on Mycobacteria tuberculosis hominis $H_{37}R_v$ and also to study the relationship of ultrastructural changes and the growth pattern in Mycobacterium tuberculosis hominis. The ultrastructural change was observed with an electron microscope while the growth pattern was studied through in vitro culture. The results are summarized as follows: 1. The ultrastructural changes found in the group treated only with isoniazid were the loss of nuclear materials and the appearence of electron dense granules. 2. In the group treated with paraaminosalicylic acid, thickening of nuclear filaments and meso some arrangement disorders were observed. 3. In the group treated with streptomycin, the ribosome particles appeared indistinct and the cytoplasm was denaturalized. 4. In the group cross treated with all three agents, all the ultrastructural changes mentioned above could be observed in the cell just as they appeared in the single treated groups. 5. In all of the single and in the crossly treated group, there were no significant changes note in the cell wall or cytoplasmic membranes of any of the cells observed. 6. In the cultural data in vitro, through the crossly treated group and single treated group. growth was observed in 3-5 weeks of culture.

• Journal of Medicine and Life Science
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• 제21권3호
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• pp.121-126
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• 2024

Disseminated tuberculosis (TB), resulting from the hematogenous spread of tubercle bacilli, typically affects immunocompromised individuals, such as those infected with the human immunodeficiency virus. However, risk factors in immunocompetent populations are not well understood. Here, we report a rare case of disseminated TB with CD4+ T-cell depletion in a previously healthy 35-year-old man. The patient presented with a 2-month history of intermittent gross hematuria, dysuria, loose stools, and weight loss. His medical history was unremarkable except for a herpes zoster infection 4 years prior to presentation. Laboratory tests revealed microscopic hematuria and pyuria; however, the urine culture was negative. Urine specimens tested positive for TB-polymerase chain reaction. Abdominal computed tomography revealed a focal filling defect in the left kidney, segmental wall thickening of the terminal ileum, and multiple enlarged lymph nodes with central necrosis. Chest computed tomography revealed active pulmonary TB. Colonoscopy confirmed intestinal TB in the terminal ileum and ileocecal valve, with positive TB-polymerase chain reaction results from sputum and ileal ulcer tissue. The patient was diagnosed with disseminated TB and was treated with standard anti-TB drugs. Although the human immunodeficiency virus test results were negative, the patient's CD4+ T-cell count was significantly low (278/μL). Follow-up tests after 1 month showed negative TB cultures; however, the patient's CD4+ T-cell depletion persisted, with counts remaining low after 1 year. This case highlights the rare occurrence of disseminated TB in immunocompetent individuals with CD4+ T-cell depletion and emphasizes the importance of CD4+ T-cell assessment in healthy patients presenting with disseminated TB.

• Journal of the Korean Wood Science and Technology
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• 제34권6호
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• pp.1-11
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• 2006

접목 과정에서 형성되는 새로운 2차목부의 특징을 광학현미경 및 전자현미경적 차원에서 조사하고자 해송(Pinus thunbergii)을 사용하여 자가접목(autograft)을 실시하였다. 조직학적으로 접목은 괴사층의 형성, 치유조직(callus)의 형성 및 발달, 새로운 형성층의 분화 및 새로운 2차조직의 형성 등 4단계를 거치는 것으로 나타났다. 접목 초기에 형성된 괴사층은 펙틴과 리그닌 성분으로 구성되어 있었으며, 점진적으로 분해되어 대목과 접수가 결합하는 시점에 소멸되었다. 접목 초기에 축적되었던 전분 입자와 지질은 접목이 진행될수록 점진적으로 감소하였으며 세포질 내 소기관, 핵 및 원형질연락사의 변화는 관찰되지 않았다. 유세포로 구성된 치유조직으로부터 접목 15일후부터 새로운 형성층(neocambium)이 발달하였으며, 이곳에서 새로운 2차조직이 형성되기 시작했다. 새로운 2차목부는 형성층에서 기원하는 정상적인 2차목부와는 다른 분화 및 형태를 나타냈다. 새로운 2차목부는 망상 또는 벽공상의 비후에 의한 2차벽을 갖는 가도관 요소에서 기원하였으며, 2차벽의 형성은 비후된 세포벽 사이를 충전시키는 형태로써 진행되었다. 2차벽의 형성은 불균일하고 간헐적이었으며 형성된 2차벽의 두께는 불균일하였다. 새로운 2차목부의 가도관은 S자 또는 소용돌이 모양을 지니는 것으로 수평 방향으로 배열되었다.

• 한국동물위생학회지
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• 제24권1호
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• pp.43-50
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• 2001

Swine proliferative enteritis(SPE) caused by inかsoma intracellularis is a common enteric disaese of grower and finisher pig. Swine affected with SPE show variable clinical signs including diarrhea, weight loss, aberrant growth and death. The characteristic lesion of ileitis at necropsy is marked thickening of the last section of the small intestine. The inner lining of the thickened intestine proliferates almost like a cancer and curved rod bacteria(L intracellularis) are always seen inside the intestinal wall. Infected swine shed the organism in the feces. Isolation and growth of pure L intracellularis in vitro requires a suitable cell culture. This procedure is difficult and not a practical means of diagnosis, thus the polymerase chain reaction(PCR) test of feces can be used to determine whether a pig is shedding the infective organism. A sensitive assay based on amplification of a 319bp DffA fragment of the L intracellularis of Swine proliferative enteritis was attempted for the detection of the organism in the 62 feces of swine. L intracellularis was identified on three herds and detected in 6 fecal samples, representing a infection rate of 9.7%. The PCR was very sensitive and specific on the individual level. The PCR technique could be very useful for the diagnosis of this disease.

• Journal of Gastric Cancer
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• 제11권4호
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• pp.234-238
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• 2011

A 67 year old male at a regular checkup underwent esophagogastroduodenoscopy. On performing esophagogastroduodenoscopy, a lesion about 1.2 cm depressed was noted at the gastric angle. The pathology of the biopsy specimen revealed a well-differentiated adenocarcinoma. On performing an abdominal computed tomography (CT) scan & positron emission tomography-computed tomography (PET-CT) scan, no definite evidence of gastric wall thickening or mass lesion was found. However, lymph node enlargement was found in the left gastric and prepancreatic spaces. This patient underwent laparoscopic assisted distal gastrectomy and D2 lymph node dissection. On final examination, it was found out that the tumor had invaded the mucosal layer. The lymph node was a metastasized large cell neuroendocrine carcinoma with an unknown primary site. The patient refused chemotherapy. He opted to undergo a close followup. At the postoperative month 27, he had a focal hypermetabolic lesion in the left lobe of the liver that suggested metastasis on PET-CT scan. He refused to undergo an operation. He underwent a radiofrequency ablation.