• Korean Journal of Veterinary Research
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• v.30 no.1
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• pp.107-112
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• 1990

The purpose of this study is to derive indirect values of volume of packed red cell(VPRC), without centrifugation, from erythrocyte sedimentation rate(ESR) of blood diluted with isotonic dextrose solution in cattle. Each of ten blood samples taken from apprently healthy Korean cows and Holstein cows was used to produce eight different mixture of autologous plasma and blood corpuscles such that their values of VPRC lay between 15 to 50ml/100ml, and then each of the blood mixture was again diluted with 5% dextrose solution. The measurements of ESR using 45 degree-angled capillary hematocrit tube, 1.1~1.2mm bore, ($45^{\circ}$-micro-ESR) were practised for the diluted blood of various levels of VPRC under the ambient temperature of $10^{\circ}$, $20^{\circ}$ and $30^{\circ}C$. Reliable values of VPRC on the basis of the correlating linear regressive equation to the ESR could be derived from the values of $45^{\circ}$-micro-ESR/hr in the mixture of one part of whole blood and one part of 5% dextrose solution(1:1). For an aid of practical use, authors suggested a list of the $45^{\circ}$-micro-ESR/hr values of the diluted blood equivalent to VPRC of whole blood.

• Korean Journal of Veterinary Research
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• v.29 no.2
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• pp.41-49
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• 1989

The study was undertaken to obtain indirect values of volume of packed red cell (VPRC) without centrifugation, using the erythrocyte sedimentation rate (ESR) of diluted blood in dogs. ESRs of diluted blood using the diluent of autologous plasma in which formed numerous RBC-rouleau clumps, autologous serum in which formed a few RBC-rouleau clumps, and 5% dextrose or 6% sucrose solutions in which formed numerous RBC-aggregation clumps, were accelerated. But, ESR of diluted blood using the 0.9% saline, D-S, ACD-B, CDP or D-PBS solutions were sluggish, because erythrocytes were dispersed in these diluents. Reliable values of VPRC on the basis of the correlating regressive equation to the ESR could be derived from values of$60^{\circ}$-angled-micro-ESR/40 min in the mixture, four parts of 5% dextrose solution and one part of whole blood. In the ESR values of diluted blood with low ratio, 1:1~3:1, $60^{\circ}$-micro-ESR was higher than $60^{\circ}$-Wintrobe-ESR. But, in the diluted blood with high ratio, 4:1~5:1, there was no different ESR values. For an aid of practical use, authers suggested a list of the $60^{\circ}$-micro-ESR/40 min in the diluted blood with equivalent VPRC of whole blood.