• Journal of Animal Environmental Science
• /
• v.18 no.3
• /
• pp.137-144
• /
• 2012

This study was conducted to determine the effect of feed additives including probiotics, moisture and feed ingredients and the effect of fermented feed on digestibility and volatile fatty acid (VFA) level in finishing pigs. Feed was mixed with microbials including Saccharomyces, Lactobacillus, Enterococcus and Pediococcus together with different levels of probiotics, 0.25, 0.5, 1, 2, 3%. Addition of probiotics showed improved fermentation rate after 48 h incubation. To determine the optimal moisture level for fermentation, different levels of water, 30, 40, 50 and 60%, were added into the feed. Fermentation rate of feed with 40~50% moisture level was higher than that from 60% level at 60 h post-fermentation. In vitro fermentation rate of feed ingredients was analyzed by comparing VFA levels. Beet pulp and tapioca showed higher fermentation rate compare to other ingredients including canola meal or rapeseed meal. To determine the effect of administration of fermented feed In vivo, feces from finishing pigs were analyzed. Finishing pigs administrated with fermented feed showed improved digestibility and higher volatile fatty acid (VFA) level. In conclusion, results from the current study indicate that 40~50% of moisture with addition of beet pulp and tapioca in feed is optimal condition for fermentation. Furthermore, our data suggest that fermentation of feed can improve the feed quality and digestibility, thereby provide more nutrient in finishing pigs.

• Asian-Australasian Journal of Animal Sciences
• /
• v.17 no.9
• /
• pp.1286-1290
• /
• 2004

This study was carried out to screen yeasts effective in reducing odor from pig feces. Three isolates from soil and compost sources were selected to treat pig feces. On the basis of morphological and biochemical characteristics, one isolate from compost was Candida rugosa, and two isolates from soil were Candida rugosa and Candida maris. These isolates showed deodorizing activity by reducing the concentration of ${NH}_3$ and R-${NH}_2$. Volatile fatty acids (VFA) are the specific malodorous compounds of pig feces, and the Candida maris from soil showed a 100% reduction of butyric, iso-butyric, and iso-valeric acid in 10% pig slurry medium. However, the Candida rugosa from compost showed a 100% reduction of butyric and iso-butyric acid while that from soil showed a 100% reduction of propionic, butyric acid and iso-valeric acid in the medium. Also, these yeasts were effective in reducing $NH_{4}-N$, soluble-N, and biological oxygen demand (BOD).

• Asian-Australasian Journal of Animal Sciences
• /
• v.33 no.11
• /
• pp.1779-1786
• /
• 2020

Objective: To evaluate soy sauce oil (a by-product of making whole soybean soy sauce) as a new dietary lipid source, a large amount of soy sauce oil was administered into the rumen of dairy cows. Methods: Four Holstein dairy cows fitted with rumen cannulae were used in a 56-day experiment. Ruminal administration of soy sauce oil (1 kg/d) was carried out for 42 days from day 8 to day 49 to monitor nutritional, physiological and production responses. Results: Dry matter intake and milk yield were not affected by soy sauce oil administration, whereas 4% fat-corrected milk yield and the percentage of milk fat decreased. Although ruminal concentration of total volatile fatty acids (VFA) and the proportion of individual VFA were partially affected by administration of soy sauce oil, values were within normal ranges, showing no apparent inhibition in rumen fermentation. Administration of soy sauce oil decreased the proportions of milk fatty acids with a carbon chain length of less than 18, and increased the proportions of stearic, oleic, vaccenic and conjugated linoleic acids. Conjugated linoleic acid content in milk became 5.9 to 8.8 times higher with soy sauce oil administration. Blood serum concentrations of non-esterified fatty acid, 3-hydroxybutyric acid, total cholesterol, free cholesterol, esterified cholesterol, triglyceride and phospholipid increased with administration of soy sauce oil, suggesting a higher energy status of the experimental cows. Conclusion: The results suggest that soy sauce oil could be a useful supplement to potentially improve milk functionality without adverse effects on ruminal fermentation and animal health. More detailed analysis is necessary to optimize the supplementation level of this new lipid source in feeding trials.

• Asian-Australasian Journal of Animal Sciences
• /
• v.20 no.3
• /
• pp.365-372
• /
• 2007

This study was conducted to determine effects of salinomycin (SL) on feeding-induced changes in glucose kinetics and blood VFA concentrations in sheep fed a high-roughage diet. Four sheep were fed the diet with or without 20 mg/kg diet of SL once daily for 21 d. Glucose entry and utilization rates were determined during the prefeeding and 3 h postfeeding periods, using a [$^{13}C_6$]glucose dilution method and non-steady state equations. Ruminal characteristics and concentrations of blood VFA, plasma glucose and insulin were also measured during the same periods. A feeding-induced increase in ruminal total VFA concentration tended to be inhibited (p<0.10) with SL, although ruminal pH was unaffected (p>0.10) with SL or by feeding. Salinomycin decreased (p<0.05) acetate proportion and increased (p<0.05) propionate proportion in the rumen, but did not modify these changes in response to feeding (p>0.10). A feeding-induced increase in blood acetate concentration was attenuated (p<0.05) with SL. Salinomycin tended to increase (p<0.10) blood propionate concentration without modifying its response patterns to feeding (p>0.10). Plasma concentrations of glucose or insulin were unaffected (p>0.10) with SL. Salinomycin tended to enhance (p<0.10) glucose entry and utilization rates. Feeding also enhanced (p<0.01) both rates, whereas their interactive effect was not detected (p>0.10). We conclude that SL possibly enhances whole body glucose entry and utilization with an increase in blood propionate concentration in sheep given a high-roughage diet, although SL does not appear to affect their responses to feeding.

• Journal of Animal Science and Technology
• /
• v.61 no.1
• /
• pp.18-27
• /
• 2019

With South Korea's limited capability of feed production because of its relatively small cultivable area, the country is pushed to depend on foreign feed imports despite the immensely fluctuating price of corn. Hence, intensive efforts to increase the total cultivable area in Korea like extending of farming to mountainous area is being practiced. Corn was planted in Goesan County, a mountainous area in the country. Grain and stover were harvested separately in three harvest periods: early-harvest (Aug 8), mid-harvest (Aug 18), and late-harvest (Aug 28). The nutrient composition such as dry matter (DM), crude protein (CP), crude fat (EE), organic matter (OM), neutral detergent fiber (NDF), acid detergent fiber (ADF), and non-fibrous carbohydrates (NFC) was determined after harvest. Effective degradability (ED) of the major nutrients (DM, NDF, ADF, and CP) were measured through in vitro fermentation of rumen fluid from Hanwoo (Korean cattle). pH, ammonia-N concentration, volatile fatty acid (VFA) concentration, and gas production were periodically measured at 0, 3, 6, 12, 24, 48, and 72 h. Corn grain showed higher nutrient content and ED than stover. It also had higher gas production but its pH, ammonia-N, and total VFA concentration were lower than corn stover. The best nutrient composition of corn grain was observed in early-harvest (high CP, EE, NDF, OM, NFC, and low ADF). Early-harvest of corn grain also had high effective degradability of dry matter (EDDM), effective degradability of neutral detergent fiber (EDNDF), effective degradability of acid detergent fiber (EDADF), and total VFA concentration. On the other hand, the best nutrient composition of stover was observed in mid-harvest (high DM, CP, NDF, and low ADF). EDDM, EDNDF, and EDADF were pronounced in early-harvest and mid-harvest of stover but the latter showed high total VFA concentration. Hence, early and mid-harvested corn stover and grain in a mountainous area preserved their nutrients, which led to the effective degradation of major nutrients and high VFA production.

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.3
• /
• pp.350-353
• /
• 2005

Effects of sucrose supplement on the pattern of VFA production and microbial protein synthesis in the rumen were examined in sheep consuming basal diet of grass silage (2.5 kg fresh wt/d) that was provided in 24 equal meals each day by an automatic feeder. Four mature wethers were allocated to four experimental treatments in a 4${\times}$4 Latin square design with periods lasting 14 days. The treatments were (1) the basal diet, (2) supplemented with 150 g sucrose and 7.0 g urea, (3) 300 g sucrose and 13 g urea, and (4) 450 g sucrose and 20 g urea given as a continuous intraruminal infusion for 24 h. All infusions were given in 2 litres of aqueous solution per day using a peristaltic pump. The effect of sucrose level on rumen mean pH was significantly linear (p<0.01). There were not significant differences in the concentration of ammonia-N, total VFA and the molar proportions of acetate, propionate and butyrate with the level of sucrose infusion. The molar proportions of isobutyric acid (p<0.05) and isovaleric acid (p<0.001) were significantly reduced when the infused amount of sucrose was increased. The flow of microbial N was linearly (p<0.001) increased with sucrose and urea level. High levels of readily fermentable carbohydrate in a ration reduced the efficiency of microbial protein synthesis in the rumen. It was demonstrated that of the individual fatty acids, only the molar proportion of isovalerate showed a significant negative correlation (R2=$0.3501^{**}$) with the amount of microbial N produced and a significant positive correlation (R2=$0.2735^{**}$) with the efficiency of microbial growth.

• Korean Journal of Veterinary Research
• /
• v.35 no.4
• /
• pp.823-831
• /
• 1995

The composition of rumen volatile fatty acids(VFA) and the blood chemistry were investigated in 5 clinically health dairy cows(Group I) reared on dairy farms and in 5 cows with post-parturition(POP) primary ketosis(Group II). The determinations were performed on days 5 to 7 pre-parturition(PRP), immediately POP, and on days 5, 10, 15, 20 and 25 POP. In both groups, the total VFA levels gradually increased starting from day 5 POP, but the levels were lower in Group II than in Group I. With regard to POP. changes in the composition of VFA, Group II occasionally showed lower levels of acetic acid and caproic acid than did Group I. Blood glucose levels decreased POP in both groups. In contrast, blood levels of ketone bodies and 3-hydroxybutyric acid were increased POP, but there was no statistically by significant difference between the groups. The aspartate aminotransferase level was transiently increased immediately POP in both groups, and the increase was more marked in Group II than in Group I. Both groups showed a tendency for total cholesterol, free cholesterol, ester cholesterol, phospholipid, and total bile acid to be increased POP, but there was no statistically significant difference between the groups. Clinically healthy dairy cows also showed POP changes in the composition of VFA and blood similar to those in dairy cows with ketosis, suggesting that even apparently healthy cows are at risk of subclinical ketosis POP.

• Journal of Animal Environmental Science
• /
• v.3 no.1
• /
• pp.1-12
• /
• 1997

This study was conducted to investigate the combined effects of VFA composition of rumen fluid and heat exposure (30${\pm}$2$^{\circ}C$) on the general clinical view and insulin secretory response to glucose in sheep. The total infusion of nutrients was examined in sheep via the technique of continuous alimentation. Four adult Suffolk sheep fitted with a permanent ruminal cannula and a simple T-shaped duodenal cannula were used. A peristaltic pump was used to infuse the solutions of volatile fatty acid triglycerides (VFA-TG) consisting of 70 triacetin : 20 tripropionin : 10 tributyrin (low propionin division: LP) and 50 triacetin : 40 tripropionin : 10 tributyrin (high propionin division: HP) on the basis of energy and minerals into the rumen, and casein solution into the duodenum. The effects of heat exposure and type of the levels of VFA-TG solutions on the insulin secretory response to glucose in sheep were investigated by using hyperglycemic clamp (HGC) technique. The results obtained are summarized as follows: 1. During the heat exposure (latter half of the infusion period), respiration rate, heart rate and rectal temperature increased (P＜0.01, P＜0.01, P＜0.05), but the levels of VFA-TG solutions (LP and HP division) did not affect the general clinical view except for the heart rate. 2. In the HGC technique, glucose infusion rate (GIR) and mean plasma insulin increments (MPII) tended to be ower in the heat exposure than in the thermoneutral environment, but no significant difference was found among the treatments. GIR and MPII remained unchanged between the levels of VFA-TG solutions. 3. In the HGC technique, ratio of MPII to GIR (MPII/GIR) which represents pancreatic ${\beta}$-cell response to glucose stimulation remained unchanged among the treatments.

• Korean Journal of Agricultural Science
• /
• v.48 no.2
• /
• pp.231-239
• /
• 2021

This study evaluated the effect of microwave-dried black soldier fly (Hermetia illucens) larvae meal (HILM) as a dietary protein source for broiler diets. A total of 250 male broilers were randomly allocated to five dietary treatment groups (10 replicates and 5 birds·pen^-1) which were respectively fed the following: a control diet (HI0) and four experimental diets in which soybean meal was replaced with 2 (HI2), 4, 6, and 8% HILM. At the end of the study (35 d), blood samples were collected from 10 randomly selected broilers (1 bird per pen) to determine serum parameters. Then, the broilers were slaughtered to determine volatile fatty acid (VFA) profiles in cecal digesta. The average daily gain and average daily feed intake linearly decreased (p < 0.01), and the feed conversion ratio linearly increased (p < 0.05) according to the inclusion level of HILM; however, there were no significant differences between the HI0 and HI2 groups. Dietary HILM increased (p < 0.01) the total VFAs in cecal digesta and decreased (p < 0.05) the triglyceride level in the blood. The broilers fed HILM had lower (p < 0.01) serum phosphorus levels; dietary HILM increased (p < 0.05) the serum calcium level. The total VFAs in cecal digesta were positively influenced by the dietary microwave-dried HILM. However, a low inclusion level (2%) of HILM in broiler diets is proper in terms of growth performance and health.

• New & Renewable Energy
• /
• v.5 no.4
• /
• pp.80-85
• /
• 2009

The effects of various organic wastes on anaerobic fermentative hydrogen production were studied using enriched mixed microflora in batch tests. Rotten fruit, corn powder and organic wastewater enriched with sulfate (up to 1,000 mg/L) were used for experiments. Maximum hydrogen production (547.1 mL) was observed from rotten apple with initial substrate concentration of 132.2 g COD/L. The experimental result on sulfate enriched organic wastewater indicated that hydrogen production is not adversely influenced by relatively high sulfate concentration. Residual sulfate content remained at 96-98 % after 75 hours of reaction, which showed that no major sulfate reduction was occurred at pH 5.3-5.5 in the reactor. The volatile fatty acid (VFA) fractions produced during the reaction was in the order of butyrate > acetate > propionate in all experiments. The results of this study would be useful for controlling the conditions on fermentative hydrogen production using different feedstocks.