• 제목/요약/키워드: viviparous germination

검색결과 38건 처리시간 0.028초

벼 수발아시 종실내 효소활성의 품종간 차이 (Varietal Difference in Enzyme Activities during Preharvest Germination of Rice)

  • 고종철;김보경;이규성;최원영;최혜란;조은애;윤성중
    • 한국작물학회지
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    • 제50권6호
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    • pp.378-383
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    • 2005
  • 수발아는 벼 재배에서 주요한 문제 중의 하나이다. 본 연구는 수발아시 배유 저장 양분의 이용능력과 항산화 효소의 수발아성과의 관련성을 조사하기 위하여 실시하였다. 배유 저장양분 이용능력과 항산화 효소를 포함하는 효소의 활성은 수발아 중인 종자와 완전 성숙 건조한 종자에서 측정하였다. 효소 활성을 조${\cdot}$중만생 품종으로부터 수발아성이 서로 다른 각각 4개의 품종을 비교하였다. 그 결과를 요약하면 다음과 같다. 1. 중만생종에서 출수후 40일된 종실의 수발아율은 남평벼, 퐁안벼, 호안벼, 동진벼 순으로 높았으며 동진벼의 수발아율은 약 $62\%$였다. 조생종에서 수발아율은 운봉벼, 오대벼, 대관벼, 대성벼 순으로 높았으며 대성벼의 수발아율은 약 $59\%$였다. 2. $\alpha$-amylase 활성은 수발아율과 상관관계가 있었으며 출 수 후 40일된 종파의 $\alpha$-amylase 활성은 중만생종에서는 동진벼자 가장 높았고 조생종에서는 대성벼가 가장 높았다. 또한, 완전 성숙한 건조 종자의 $\alpha$-amylase 활성은 수발아성이 높은 품종에서 더 높았다. $\beta$-amylase활성은 $\alpha$-amylase활성에 비하여 매우 낮았으며, 조${\cdot}$중만생 품종 모두에서 $\beta$-amylase 활성과 수발아성 사이에는 일정한 관련성이 없었다. 3. 수발아 종자와 완전 성숙한 건조 종자의 glucose 함량은 수발아성이 높은 품종에서 더 높았다. 4. peroxidase 활성은 조${\cdot}$중만생종 모두에서 수발아율과 밀접한 관련성을 보였으나 Catalase 활성은 수발아성과 관련성을 보이지 않았다.

협채용 강낭콩의 채종기술 확립 (Technology of Good Quality Seed Production in Snap-bean (Phaseolus vulgaris L.))

  • 권철상;황영현
    • Current Research on Agriculture and Life Sciences
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    • 제22권
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    • pp.1-12
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    • 2004
  • Pod-edible bean or snap bean is a fairly new crop to domestic farmers but the national demand is steadily increasing in recent years along with the development of western food business and change in dietary patterns. At the same time, much efforts are being made to export it to foreign country, mainly to Japan. The amount of seeds introduced from outside is also continuously increasing along with the enlargement of area planted for the crop. Hybridization breeding for the crop has already been started to supply the cheaper and better seeds which will reduce the seed costs and foster the higher income to the farmers. In this experiment, several technologies related with the production of quality seeds are preliminary investigated. Some of the results obtained are summarized as follows; 1. Highly significant interaction was recognized between planting dates and no. of pods per plant and no. of branches but no interaction between planting dates and plant height and no. of nodes on main stem. Days to maturity was proportionally reduced to later planting dates. 2. Rate of viviparous pods and seeds was gradually increased in later planting dates but rate of germination was increased in earlier planting dates with lower germination rate in white seed coat grains than in colored seed ones. 3. Seed yield was higher in the earlier planting dates with a great deal of varietal difference. Early to mid April was considered to he the optimum planting dates for snap bean in Kyungbuk area. High correlation was recognized between seed yield and no. of pods per plant, no. of seeds per plant, and 100 seed weight. 4. Days to flowering was three and seven days longer in Cheongsong, high mountainous area than in Kunwi, somewhat prairie lowland. One hundred seed weight was also higher in Cheongsong than in Kunwi. Rate of viviparous grains, pods, and decayed seeds was higher in Cheongsong but, at the same time, the rate of germination and seed yield was also higher in Cheongsong. 5. One hundred seed weight of KLG5007 increased continuously up to 35days after flowering and decreased thereafter but that of KLG50027 increased to 40days after flowering and slowly reduced thereafter. The content of crude oil reached to maximum at 40 days after flowering and reduced thereafter. The rate of germination in Gangnangkong 1 was the highest, 89.3%, at 35 days after flowering and reduced thereafter while that in KLG50027 reached to maximum, 70.7%. at 40days after flowering and reduced thereafter. Thus, the optimum harvesting time for snap bean was considered to be 35~40days after flowering. 6. The snap bean pods at yellow bean stage easily became viviparous ones under saturated moisture conditions for 24 hours at $25{\sim}30^{\circ}C$. Therefore, it is recommended to harvest pods somewhat earlier than yellow-bean stage and let them do post maturing, especially when it is to be rained.

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Molecular Identification and Fine Mapping of a Major Quantitative Trait Locus, OsGPq3 for Seed Low-Temperature Germinability in Rice

  • Nari Kim;Rahmatullah Jan;Jae-Ryoung Park;Saleem Asif;Kyung-Min Kim
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2022년도 추계학술대회
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    • pp.283-283
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    • 2022
  • Abiotic stresses such as high/low temperature, drought, salinity, and submergence directly or indirectly influence the physiological status and molecular mechanisms of rice which badly affect yield. Especially, the low temperature causes harmful influences in the overall process of rice growth such as uneven germination and the establishment of seedlings, which has become one of the main limiting factors affecting rice production in the world. It is of great significance to find the candidate genes controlling low-temperature tolerance during seed germination and study their functions for breeding new rice cultivars with immense low-temperature tolerance during seed germination. In this study, 120 lines of Cheongcheong/Nagdong double haploid population were used for quantitative trait locus analysis of low-temperature germinability. The results showed significant difference in germination under low different temperature conditions. In total, 4 QTLs were detected on chromosome 3, 6, and 8. A total of 41 genes were identified from all the 4 QTLs, among them, 25 genes were selected by gene function annotation and further screened through quantitative real time polymerase chain reaction. Based on gene function annotation and level of expression under low-temperature, our study suggested OsGPq3 gene as a candidate gene controlling viviparous germination, ABA and GA signaling under low-temperature. This study will provide a theoretical basis for marker-assisted breeding.

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1999년에 발생한 기상재해 유형별 벼 수량반응조사 연구 (Yield Response of Rice Affected by Adverse Weather Conditions Occurred in 1999)

  • 주영철;임갑준;한상욱;박중수;조영철;김순재
    • 한국농림기상학회지
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    • 제2권1호
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    • pp.1-8
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    • 2000
  • 본 연구는 변색립, 수발아를 유발시킨 기상환경을 구명하고 출수기의 변색립, 등숙 중 .후기의 수발아, 생식생장기의 침관수, 황숙기의 도복에 타른 수량반응을 구명하기 위하여 피해를 받은 농가 포장 및 경기도농업기술원 포장에서 조사한 결과는 다음과 같다. 벼 출수시기에 영화변색 발생시의 기상조건은 평균 기온은 21.3-26.4$^{\circ}C$, 상대습도는 75.2-98.4%, 증발계수는 19.3, 풍속은 10.8-13.m/sec.이였으며, 영화변색비율이 63.2-65.7%, 38.3-45.2%일 때 임실비율과 등숙비율 저하로 수량이 각각 26-27%. 10-17%감소하였다. 벼 등숙 중.후기에 포장에서 연속강우에 의한 수발아 발생 기상조건은 7일간 연속강우로 상대습도가 96% 이상이였고 7일간의 평균기온은 18.9$^{\circ}C$, 최고기온은 21.9$^{\circ}C$, 최저기온은 16.8$^{\circ}C$이였으며, 수발아율이 높은 품종은 주안벼(45.5%), 진부벼(14.5%). 봉광벼(14.2%), 오봉벼(12.6%) 등 이였다. 벼 생식생장기 관수시 곁가지는 1.5-2m 수심에 1일간 관수부터 발생하였고, 3-4m 수심에 2-3일간 관수시는 m$^2$당 269-571개 발생하여 전체수량에 32-52%기여하였다. 벼 황숙기 도복시 일으켜 세움과 방치시 수량 차는 대차없었으나 방치시 미숙립 증가로 완전미비율이 세움에 비하여 중묘기계이앙답은 9.1%, 건답직파답은 12.5% 낮아졌다.

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OsDOR1, a novel glycine rich protein that regulates rice seed dormancy

  • Kim, Suyeon;Huh, Sun Mi;Han, Hay Ju;Cho, Mi Hyun;Lee, Gang Sub;Kim, Beom Gi;Kwon, Taek Yun;Yoon, In Sun
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.90-90
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    • 2017
  • Regulation of seed dormancy is important in many grains to prevent pre-harvest sprouting. To identify and understand the gene related to seed dormancy regulation, we have screened for viviparous phenotypes of rice mutant lines generated by insertion of Ds transposon in a Korean Japonica cultivar (Dongjin) background. One of the mutants, which represented viviparous phenotype, was selected for further seed dormancy regulation studies and designated dor1. The dor1 mutant has single Ds insertion in the second exon of OsDor1 gene encoding glycine-rich protein. The seeds of dor1 mutant showed a higher germination potential and reduced abscisic acid (ABA) sensitivity compared to wild type Dongjin. Over-expression of Dor1 complements the viviparous phenotype of dor1 mutant, indicating that Dor1 function in seed dormancy regulation. Subcellular localization assay of Dor1-GFP fusion protein revealed that the OsDor1 protein mainly localized to membrane and the localization of OsDOR1 was influenced by presence of a giberelin (GA) receptor OsGID1. Further bimolecular fluorescence complementation (BiFC) analysis indicated that OsDOR1 interact with OsGID1. The combined results suggested that OsDOR1 regulates seed dormancy by interacting with OsGID1 in GA response. Additionally, expression of OsDOR1 partially complemented the cold sensitivity of Escherichia coli BX04 mutant lacking four cold shock proteins, indicating that OsDOR1 possessed RNA chaperone activity.

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Effective Screening Method for Viviparous Germination of Rice

  • Ju, Young-Cheoul;Han, Sang-Wook;Park, Joong-Soo;Park, Kyeong-Yeol
    • 한국작물학회지
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    • 제45권2호
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    • pp.103-107
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    • 2000
  • The viviparity of 28 rice varieties was tested at 25 days after heading(DAH), 35DAH, and 45DAH in the laboratory and field condition for 12 days. The incubation temperature was 20/l$0^{\circ}C$ (day/night), 25/15$^{\circ}$C$ and 30/20$^{\circ}$C$ in the laboratory test, and under field water conditions in the field test. The biggest varietal difference of viviparity was found in the laboratory test when examined at 45DAH with the 6-day incubation under 25/15$^{\circ}$C$ . At this conditions the mean viviparous ratio was 32.1 % with the range of 53.9 and the variance of 259.5. In the field test, the significant varietal difference in the viviparity was also found in the lodging treatment at 45 DAH for 6 days. Correlation coefficient analysis between the field and laboratory tests was highly significant from 4 days after incubation at 45 DAH and after 6-day incubation at 35 DAH, and correlation coefficient was higher as incubation days in the laboratory and submerged days under field water became longer. Considering the correlation between the field and laboratory tests, varietal difference of viviparity and convenience of testing, the laboratory test at 45 DAH for 6-day incubation under 25/15$^{\circ}$C$ was the most efficient evaluation method for the viviparity of rice cultivar.

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