• Archives of Pharmacal Research
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• 제28권3호
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• pp.311-318
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• 2005

Oxidative stress has been reported to elevate ceramide level during cell death. The purpose of the present study was to modulate cell death in relation to cellular glutathione (GSH) level and GST (glutathione S-transferase) expression by regulating the sphingolipid metabolism. LLC­PK1 cells were treated with H$_2$O$_2$ in the absence of serum to induce cell death. Subsequent to exposure to H$_2$O$_2$, LLC-PK1 cells were treated with desipramine, sphingomyelinase inhibitor, and N-acetylcysteine (NAC), GSH substrate. Based on comparative visual observation with H202-treated control cells, it was observed that 0.5 $\mu$M of desipramine and 25 $\mu$M of NAC exhibited about 90 and $95\%$ of cytoprotection, respectively, against H$_2$O$_2$-induced cell death. Desipramine and NAC lowered the release of LDH activity by 36 and $3\%$ respectively, when compared to $71\%$ in H$_2$O$_2$-exposed cells. Cellular glutathione level in 500 $\mu$M H202-treated cells was reduced to 890 pmol as compared to control level of 1198 pmol per mg protein. GST P1-1 expression was decreased in H$_2$O$_2$-treated cells compared to healthy normal cells. In conclusion, it has been inferred that H$_2$O$_2$-induced cell death is closely related to cellular GSH level and GST P1-1 expression in LLC-PK1 cells and occurs via ceramide elevation by sphingomyelinase activation.

• 한국임상약학회지
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• 제30권1호
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• pp.59-64
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• 2020

The emergence of phosphodiesterase (PDE) 5 inhibitors gave rise to the solution for erectile dysfunction, starting with the development of sildenafil. Although their efficacy in treating erectile dysfunction has been shown, the side effects of PDE5 inhibitors, especially sildenafil, must be taken into consideration. A 64-year-old man received 100 mg of sildenafil and experienced blue vision in both eyes; however, after a day or so, his symptoms improved. The symptoms disappeared when he stopped administering sildenafil, but reappeared when the medication was re-administered. Therefore, he discontinued sildenafil treatment and was prescribed udenafil instead. After that, visual adverse events no longer occurred. Causality assessment showed that in this case, sildenafil-induced cyanopsia was "certain" under the World Health Organization-Uppsala Monitoring Center (WHO-UMC) criteria and Korean causality assessment algorithm (Ver.2), and was "probable" according to the Naranjo scale. In addition, sildenafil also led to abnormal visual reactions in other cases. Sildenafil can also inhibit PDE6, which is present in retinal cells, unlike other PDE5 inhibitors. Thus, visual adverse reactions, such as blue vision, are the unique results of sildenafil, and other PDE5 inhibitors may be used to prevent them.

• 대한전자공학회논문지SP
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• 제38권6호
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• pp.617-629
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• 2001

인간시각체계에서 단순세포의 수용영역에 대한 수학적인 모델 연구가 정신물리학, 생리학, 신경과학 측면에서 발전되어 왔다. 여러 분야에서 사용되는 모델은 Gabor 복소함수, Gaussian 미분, Hermite 함수를 포함하며, 영상처리와 컴퓨터 시각 분야에 많이 응용되고 있다. 본 논문에서는 불확실성원리에 기초하여 고려된 함수들의 결합유효폭을 계산하고, 인간시각필터와 일치되는 중심주파수와 각 함수의 변수들을 결정하고, 결과적인 대역폭을 분석한다. 더욱이 함수들의 위치와 주파수 영역의 특성을 파악하여 정신물리학적으로 획득한 실험적인 데이터와의 관련성을 검토한다.

• Molecules and Cells
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• 제46권8호
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• pp.461-469
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• 2023

The tail of the striatum (TS) is located at the caudal end in the striatum. Recent studies have advanced our knowledge of the anatomy and function of the TS but also raised questions about the differences between rodent and primate TS. In this review, we compare the anatomy and function of the TS in rodent and primate brains. The primate TS is expanded more caudally during brain development in comparison with the rodent TS. Additionally, five sensory inputs from the cortex and thalamus converge in the rodent TS, but this convergence is not observed in the primate TS. The primate TS, including the caudate tail and putamen tail, primarily receives inputs from the visual areas, implying a specialized function in processing visual inputs for action generation. This anatomical difference leads to further discussion of cellular circuit models to comprehend how the primate brain processes a wider range of complex visual stimuli to produce habitual behavior as compared with the rodent brain. Examining these differences and considering possible neural models may provide better understanding of the anatomy and function of the primate TS.

• Journal of Photoscience
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• 제9권2호
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• pp.44-46
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• 2002

In the vertebrate retina, rods mediate twilight vision and cones daylight vision. Rods have been purified easily from the retina, and thus the phototransduction mechanism in rods is now well documented. However, it has not been possible to purify cones in large quantities, and therefore, the knowledge on the mechanism in cones is limited. Here we report purification of carp (Cyprinus carpio) cones with a stepwise Percoll gradient. Using purified cells, we compared the phototransduction mechanism between rods and cones. The results showed that both transducin activation and phosphodiesterase activation are less effective, and visual pigment phosphorylation is faster in cones. These differences explain lower light-sensitivity and briefer photoresponse time course in cones.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2001년도 ICCAS
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• pp.133.4-133
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• 2001

In the paper, a Visual factory model for a optical-components manufacturing process is built. The optical-components manufacturing process is composed of 3 operation processes; optical sub assembly process, package assembly process, and fiber assembly process. Each process is managed not a batch mode, which is one of most popular manufacturing styles to produce a great deal of industrial output, but though a modular cell. In the processes, a modular cell has to be processed independently of the other cells. Optimization for the composition of assembly cell in the optical-components system is made by the Visual factory model.

• Journal of Photoscience
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• 제3권3호
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• pp.127-132
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• 1996

Calcium has a variety of functions in neuron and muscle cells and blood clotting, especially in the visual system where dark adapted rods cotransport with Na$^+$ into the cell. An influx of Ca$^{2+}$ flows out of the cell through the Na$^+$ - Ca$^{2+}$ exchanger. By using a modified Ussing chamber in order to bring in vivo environment close, we have concluded that Ca$^{2+}$ blocks the activity of guanylate cyclase; in consequence, having an effect on the amplitude of electroretinogram (ERG). We suggest that Ca$^{2+}$ moves between the photoreceptor and the vitreous humor by way of certain Ca$^{2+}$ transport mechanisms. Also, the effect of Zn$^{2+}$ in Ca$^{2+}$ - free ringer solution caused an elevation of amplitude in ERG and a reduction of threshold.

• 대한의용생체공학회:의공학회지
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• 제22권4호
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• pp.331-338
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• 2001

In this paper, we have proposed a new computer retina model reflecting the mechanism of transient amacrine cell on the basis of a conventional computer retina model to understand mechanism of visual information processing. The conventional computer retina model contained most of mechanism for other retina models and it was verified with the physiological data. However, we found that a conventional computer retina model doesn't have the mechanism of amacrine cell that was likely to respond to moving stimulus. In proposed model, therefore, a conventional computer model that considered from photoreceptors to bipolar cells and a new computer model that considered for transient amacrine cell and ganglion cell was combined. As we compared the physiological data with the results of computer simulation of transient amacrine cell about fixed stimulus and moving stimulus, we confirmed that the proposed new computer retina model was normally operated.

• 대한의용생체공학회:의공학회지
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• 제24권4호
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• pp.241-246
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• 2003

정상 성인을 대상으로 언어생성과제의 시각적 지시과제와 청각적 지시과제에 대한 기능적 MR영상들을 비교하고자 하였다. 6명의 정상 성인을 대상으로 자기공명영상기기를 이용하였다 시각적, 청각적 언어 지시과제는 명사의 언어를 이용하여. 휴지기. 확성기를 각각 6번 구성하였다. 뇌의 활성영역의 휴지기와 확성기의 신호변화에 따른 데이터는 MRDx 프로그램으로 처리하였고 cross correlation 방법으로 통계 처리하였다. 시각적, 청각적 지시과제의 분석방법은 lateralization index 공식으로 뇌 반구의 활성화 voxel의 총합과 좌$.$우 반구의 픽셀 수를 구하였고, 지시 과제에 대한 활성화 영역을 “Area of Activation Cells” 값에 따라 4등급으로 분류하여 비교 분석하였다. 시간적 청각적 지시과제에서 Broca, Wernicke 영역 등에서 크기는 다르지만 모두 활성화가 관찰되었다. lateralization index에서 뇌반구의 활성화 값에 차이는 시각적 지시과제에 비해 청각적 지시과제가 높은 활성화 값을 보였다. 그러므로 언어영역에 대한 뇌의 기능적 영상을 얻는데 두 방법 모두 유용하다고 생각된다.

• 생약학회지
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• 제36권2호통권141호
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• pp.88-92
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• 2005

Apoptosis plays an important role in autoimmune chronic (Hashimoto's) thyroiditis, a disorder that often results in hypothyroidism. The goal of this study was to induce apoptosis by the combination of inflammatory cytokines, interferon $(IFN)-{\gamma}$ and tumor necrosis factor $(TNF)-{\alpha}$, and to investigate a potential role of medicinal plants in the thyroid follicular cells (FRTL) in vitro. The apoptosis was evaluated by cellular viability, DNA fragmentation, and terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling (TUNEL) assay. Extract of Gamgung-tang (GGT, Glycyrrhizae Radix, black beans, Angelicae Radix, and Cnidii Rhizoma) $(0.3{\sim}9.0mg/ml)$ was shown to maintain the viability of cells treated with $IFN-{\gamma}(100U/ml)$ and $TNF-{\alpha}$ (0.5 ng/ml). FRTL cells were found to undergo DNA fragmentation with the inflammatory cytokines. The extract of GGT inhibited DNA fragmentation in dose-dependent manner. The cells with TUNEL-positive nuclei were detected with $IFN-{\gamma}$ and $TNF-{\alpha}$ treatment. The number of TUNEL-positive cells decreased with the treatment of extract of GGT. These results indicate that medicinal plants inhibit the occurrence of apoptosis in thyroid follicular cells, therefore, may have therapeutic potential in the treatment of autoimmune chronic thyroiditis.