• Title/Summary/Keyword: visual cells

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Visual Cell : Image Analysis and Visual Retrieval System for Biology Cell Image Bigdata (Visual Cell : 바이오세포 이미지 빅데이터를 위한 이미지 분석 및 시각적 검색 시스템)

  • Park, Beomjun;Jo, Sunhwa;Lee, Suan;Shin, Jiwoon;Yoo, Hyuk Sang;Kim, Jinho
    • The Journal of Bigdata
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    • v.4 no.1
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    • pp.53-61
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    • 2019
  • The extracellular matrix, which provides the structural and biochemical support of surrounding cells, is a cell physiological modulator that controls cell division and differentiation. In the bio sector, the company produces Scapold, a three-dimensional support for tissue engineering, and cultivates stem cells in the produced Scapold to be transplanted into animals to assess tissue regeneration. This depends on components such as collagen in the tissue. Therefore, it is very important to identify the inclusion rate and distribution of components in the tissue, and the data are obtained by analyzing the color of the dyed tissue image. The process from image collection to analysis is costly, and the data collected and analyzed are managed in different formats by different research institutions. Therefore, data integration management and analysis results search are not being performed. In this paper, we establish a database that can manage relevant bigdata in an integrated manner, and propose a bio-image integrated management and retrieval system that can be searched based on color, an important analytical measure in this field of study.

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Morphology of Retinas and Lenses in the Fish of the Genus Zacco (Cypriniformes, Cyprinidae): Possible Relationship with Prey and Habitat

  • Lim, Jae-Won;Lee, Chung-Lyul;Lee, Moo-Sam
    • Animal cells and systems
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    • v.7 no.4
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    • pp.331-335
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    • 2003
  • Vertebrates with different habitats have different proportions of visual cells, with the rod cells responding to scotopic vision and the cone cells responding to photopic and color vision in their retinas. The present work studied whether the kinds and arrangement patterns of the cone cells and interlocking morphology of the lens were related to the kind of preys and habitats in the genus Zacco. The retinas were observed by a light microscopy using H-E staining method and the interlocking formula of the lens fibers were investigated by a scanning electron microscopy. The interlocking formula of the lens fibers of Z. temmincki is an ' anchor and socket ' connection, and that of Z. platypus is a ' ball and socket ' connection. The cone cells of Z. platypus and Z. temmincki constituted compacted mosaic patterns of row type. Away from the center, the double and single cone cells gradually increased in diameter. Zacco temmincki had identical double cone cells and Z. platypus had non-identical double and single cone cells. The eyes of Z. temminckifeeding on a moving aquatic insects in relative limpid water and swift current of mid and upper stream have better resolution than that of Z. platypus feeding on mainly adhesive algae and some aquatic insects in slightly turbid water of mid stream.

Design Learning Environment based on Affordance Concept for Convergent Design Education

  • Kim, Sunyoung
    • International Journal of Advanced Culture Technology
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    • v.8 no.1
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    • pp.199-206
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    • 2020
  • I suggested the design learning environment based on affordance concept approach for supporting and improving learners' behavior and outcome for convergent design education in this study. The design learning space should be applied teaching and learning activity, especially learners' behavior, physical space condition to support the design thinking process. The design learning space needs openness, individuality and connectivity to support the learners' behavioral to immerse, participate, cooperate, understand, think and fulfill the design thinking process. The composition principles of the learning environment for convergent design education supports communication and collaboration among members for independence and interaction. The spaces for design research and teaching needs high privacy while facilitating visual communications through special materials and wall structure design. Also, for connectivity to improve the learners' physical and visual contact, the environment of the classrooms requires flexibility and mobility by providing an open space integrating unit cells for realizing learning purpose. These are provided by formed of an open structure for inducing visual communication and physical contact to involve the design activities and the mutual interchange.

Petri-Nets Modeling and Performance Evaluation of Optical-components Manufacturing System (광 부품 조립 시스템의 모델링과 성능평가)

  • 김영호;김지한;정승권;배종일;이만형
    • Proceedings of the Korean Society of Machine Tool Engineers Conference
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    • 2002.04a
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    • pp.491-495
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    • 2002
  • In the paper, a Visual factory model for a optical-components manufacturing process is built. The optical-components manufacturing process is composed of 3 operation processes; optical sub assembly process, package assembly process, and fiber assembly process. Each process is managed not a batch mode, which is one of most popular manufacturing styles to produce a great deal of industrial output, but though a modular cell. In the processes, a modular cell has to be processed independently of the other cells. Optimization for the composition of assembly cell in the optical-components system is made by the Visual factory model.

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Modeling of Optical-components Manufacturing System Using Petri-Net (페트리 네트를 이용한 광부품 조립 시스템의 모델링)

  • 김영호;차동국;정승권;배종일;이만형
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2002.05a
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    • pp.636-639
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    • 2002
  • In the paper, a Visual factory model for a optical-components manufacturing process is built. The optical-components manufacturing process is composed of 3 operation processes; optical sub assembly process, package assembly process, and fiber assembly process. Each process is managed not a batch mode, which is one of most popular manufacturing styles to produce a great deal of industrial output, but though a modular cell. In the processes, a modular cell has to be processed independently of the other cells. Optimization for the composition of assembly cell in the optical-components system is made by the Visual factory model.

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Characterization of Conduction Mechanism in Cu Schottky Contacts to p-type Ge

  • Kim, Se Hyun;Jung, Chan Yeong;Kim, Hogyoung
    • Transactions on Electrical and Electronic Materials
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    • v.15 no.6
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    • pp.324-327
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    • 2014
  • Germanium (Ge) is a promising material for next generation nanoelectronics and multiple junction solar cells. This work investigated the electrical properties in Cu/p-type Ge Schottky diodes, using current-voltage (I-V) measurements. The Schottky barrier heights were 0.66, 0.59, and 0.70 eV from the forward ln(I)-V, Cheung, and Norde methods, respectively. The ideality factors were 1.92 and 1.78 from the forward ln(I)-V method and Cheung method, respectively. Such high ideality factor could be associated with the presence of an interfacial layer and interface states at the Cu/p-Ge interface. The reverse-biased current transport was dominated by the Poole-Frenkel emission rather than the Schottky emission.

Bilateral Optic Neuritis after Measles Infection (홍역 감염 후 발생한 양측성 시신경염 1례)

  • Hwang, Sung Hyun;Kim, Jong-Hyun;Oh, Jin Hee;Koh, Dae Kyun
    • Pediatric Infection and Vaccine
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    • v.11 no.2
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    • pp.192-197
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    • 2004
  • A 9-year-old boy who was confirmed measles by clinical manifestations and serum measles IgM antibody presented with bilateral visual loss 12 days after the onset of maculopapular rash. Complete ophthalmic and neurologic examinations, radiologic studies, and lumbar puncture were performed. Visual acuities were counting fingers in both eyes, with mild bilateral optic disk hyperemia and swelling noted. Neurologic examination was unremarkable, however, a magnetic resonance imaging of the brain showed high signals on basal ganglia, and periventricular white matter. The cerebrospinal fluid was devoid of white cells. Intravenous methylprednisolone and high dose immunoglobulins were administered, and clinical findings resolved completely within 6 months.

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Ultrastructure of Stemmata in Cabbage Butterfly, Pieris rapae L. (배추흰나비 옆홑눈의 미세구조)

  • Kim, Chang-Shik
    • Applied Microscopy
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    • v.25 no.4
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    • pp.9-16
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    • 1995
  • Ultrastructure of stemmata(larval eye) of 5th-instar larval in cabbage butterfly, Pieris rapae L, was morphologically investigated with light microscope, scanning electron microscope and transmission electron microscope Six stemmata are on each side of the head. Stemmata V and VI have a Y-shaped sulcus on the surface of their corneal lenses, the others have a columnar shaped process and smooth globular surface. The visual type of stemmata is resembled a single ommatidium of compound eye. The dioptric apparatus are a biconvex shaped cornea and crystalline cone. As a photoreceptor, each stemmata consists of 7 retinular cells arranged into 2 tiers. The first ceil tier of 3 distal retinular cells has formed a V-shaped cup rhabdome and the second cell tier of 4 basal retinular cells has formed a H-shaped fused rhabdome. Each retinular cell filled with pigment granules and contained multivesiclular bodies, coated vesicle and common organelles. The peripheral parts of retinular cells are enveloped by neuroglia cells and retinular cells are surrounded by 3 corneagenous cells. The distal portions of the 3 corneagenous cells contact each other, but the Y-shaped stemmata is separated from each other immediately under the cornea. The 7 axons from each stemma congregate into a bundle and each 7-axon group joins to form a stemmatal nerve, consisting of 42 retinular axons.

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Studies of Opsin Genes in a Smelt Fish, Ayu (Plecoglossus altivelis)

  • Minamoto, Toshifumi;Shimizu, Isamu
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.269-271
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    • 2002
  • To investigate the visual and extra-ocular photoreception, we cloned the opsin genes in ayu (Plecoglossus allivelis). Amplified fragments encoding exon-4 (-5) of opsin cDNAs were cloned from the retina and brains of ayu, and sequenced. One clone was identified as rod (AYU-Rh), two as green cone (AYU-GI, -G2), one as red cone (A YU-R), two as ultraviolet cone (AYU-UVl, UV2), one as VA (AYU-VA), and one as extra-ocular rod (AYU-ExoRh) opsins. 335 amino acids sequence deduced from the full-length cDNA of AYU-Rh showed high identity with that of other fish. Southern blotting analysis indicated that ayu possess two 'rhodopsin' genes, one is visual rhodopsin and the other is non-visual extra-ocular rhodopsin. In situ hybridization showed that the mRNA of AYU-Rh was localized only in rod cells in the retina. On the other hands, AYU-ExoRh was expressed only in the pineal. We cloned two isoforms (AYU-VAM and -VAL) of VA opsin from ayu. The deduced amino acid sequences of these variants were identical to each other within the first 342 residues, but they showed divergence in the C-terminal sequence. AYU- VAL corresponded to the long isoform found in other fish, and AYU-VAM was identified as a new type of VA opsin variant. Pal-VAM is a new probably functional non-visual photoreceptive molecule in fish.

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Study for the Production of Immunodeficiency Animal for Xenotransplantation

  • D. I. Jin;Lee, S. H;J. H. An;Y. G. Ko;Kim, H. J.;Lee, S. H.;Park, C. S.
    • Korean Journal of Animal Reproduction
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    • v.26 no.4
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    • pp.347-351
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    • 2002
  • Transgenes in HSY-TK gene driven by the lck promoter was tested for the expression in immune cells (Jurkat cells) to apply xenotransplantation of human cells into transgenic animals for the potential use of the proliferation or differentiation of human stem cells in the large animal such as an pig. Also, lck-CFP gene was used for transfection experiment into Jurkat cell to confirm the proper regulation of lck promoter for transgene expression in the T cells. Transfection of lck-GFP gene into Jurkat ceils induced CFP expression in transfected cells. The expression of Ick-TK and Ick-CFP genes was confirmed by RT-PCR using RNAs extracted from Jurkat cells, When Jurkat cells transfected with TK and CFP genes were selected against G418 or gancyclovir treatments, Jurkat cells transfected with TK gene were not proliferated in G4i8 and gancyclovir medium while intact cells or cells transfected with CFP gene could grow in gancyclovir medium. However, Jurkat cells transfected with TK or GFP gene were proliferated in G418 medium probably due to Neo$^{r}$ gene in the vector. Gancyclovir treatment destroyed Jurkat cells expressing TK gene indicating that T-cells expressing TK gene can be selectively eliminated by TK gene expression driven by lck promoter.