• Title/Summary/Keyword: visual cells

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Freeze/Thaw cycle effects on GDLs and MEAs of PEFC (동결/해동 열사이클이 PEFC의 GDL/MEA에 미치는 영향)

  • Lim, Nam-Yun;Park, Gu-Gon;Park, Jin-Soo;Yoon, Young-Gi;Lee, Won-Yong;Lim, Tae-Won;Kim, Chang-Soo
    • 한국신재생에너지학회:학술대회논문집
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    • 2006.06a
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    • pp.96-98
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    • 2006
  • Proper water management is vital to achieve high performance and durability of PEFC (Polymer Electrolyte Fuel Cell). The effects of the residual water from PEFC after purge in shut-down processes on GDL/MEAs were investigated with freeze/thaw cycles Freeze/thaw cycle tests were conducted with single cells which were designed from transparent acryl plates. Single cells which contain several amount of residual water were cycles from $80^{\circ}C$ to $-28^{\circ}C$. The resistance changes of the single cells which have various amount of residual water were evaluated by ac-impedance analysis with 24 times of freeze/thaw cycles. Also, after the freeze/thaw cycles, the property changes were characterized by visual methods such as SEM, EPMA. Though it was difficult to observe noticeable property changes in the visual characterizations, the resistance of cells dramatically increased with the amount of remained water.

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Visual Cells in the Retina of Iksookimia longicorpa (Pisces; Cobitidae) of Korea (한국산 미꾸리과 어류 왕종개 Iksookimia longicorpa 망막의 시각세포)

  • Kim, Jae Goo;Park, Jong Young
    • Korean Journal of Ichthyology
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    • v.27 no.4
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    • pp.257-262
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    • 2015
  • The visual cells in the retina of Iksookimia longicorpa (Pisces, Cobitidae) were investigated by light and scanning electron microscopes. The retina ($216.42{\pm}13.36{\mu}m$) has several layers, and the visual cell layer consists of unequal double cones and large rods. In a double cone, two members are unequal such that one cone is longer than the other (long element $26.42{\pm}1.7{\mu}m$, short element $16.82{\pm}1.1{\mu}m$). The cones form a row mosaic pattern in which the partners of double cones are linearly oriented with a large rod. The visual cells observed have an outer segment (hematophilic), inner segment (eosinophilic). In scanning electron microscopy, the outer segment links to inner segment by so-called calyceal piles (calyceal processes) of membrane discs surrounded by double membranes.

Information Processing in Primate Retinal Ganglion

  • Je, Sung-Kwan;Cho, Jae-Hyun;Kim, Gwang-Baek
    • Journal of information and communication convergence engineering
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    • v.2 no.2
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    • pp.132-137
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    • 2004
  • Most of the current computer vision theories are based on hypotheses that are difficult to apply to the real world, and they simply imitate a coarse form of the human visual system. As a result, they have not been showing satisfying results. In the human visual system, there is a mechanism that processes information due to memory degradation with time and limited storage space. Starting from research on the human visual system, this study analyzes a mechanism that processes input information when information is transferred from the retina to ganglion cells. In this study, a model for the characteristics of ganglion cells in the retina is proposed after considering the structure of the retina and the efficiency of storage space. The MNIST database of handwritten letters is used as data for this research, and ART2 and SOM as recognizers. The results of this study show that the proposed recognition model is not much different from the general recognition model in terms of recognition rate, but the efficiency of storage space can be improved by constructing a mechanism that processes input information.

Face recognition using a sparse population coding model for receptive field formation of the simple cells in the primary visual cortex (주 시각피질에서의 단순세포 수용영역 형성에 대한 성긴 집단부호 모델을 이용한 얼굴이식)

  • 김종규;장주석;김영일
    • Journal of the Korean Institute of Telematics and Electronics C
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    • v.34C no.10
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    • pp.43-50
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    • 1997
  • In this paper, we present a method that can recognize face images by use of a sparse population code that is a learning model about a receptive fields of the simple cells in the primary visual cortex. Twenty front-view facial images form twenty persons were used for the training process, and 200 varied facial images, 20 per person, were used for test. The correct recognition rate was 100% for only the front-view test facial images, which include the images either with spectacles or of various expressions, while it was 90% in average for the total input images that include rotated faces. We analyzed the effect of nonlinear functon that determine the sparseness, and compared recognition rate using the sparese population code with that using eigenvectors (eigenfaces), which is compact code that makes contrast with the sparse population code.

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The Effect of Laser Irridation on the Ultrastructure of Retina (Laser 조사가 망막의 미세구조에 미치는 영향)

  • Kim, Douk Hoon;Mun, Jung Hak
    • Journal of Korean Ophthalmic Optics Society
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    • v.1 no.1
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    • pp.15-22
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    • 1996
  • The fine structure of retinal tissue was studied to investigate on effect of Laser irridation on the ICR mouse with electron microscope. The results obtained were as follows: 1. At the normal groups, the most retinal layers were a complex structure, consisting of several specific cells and nerve fiver. 2. In the increasing time of Laser irridation, each cell layer of retina was not uniform of the structure and band. The visual cells were severely heterochromatin swelling of cytoplasm, irregular shape & heterochromatin of nuclear, and disappear of some cytoplasm. The nucleus and nerve fiber of retinal layer was a very irregular shape, formation of vesicle, not identify of each intercellular boundary. The pigment epithelial cells were not an uniform, a large vesicle formation of cytoplasm, and a condensation & very irregular shape of nucleus.

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Study on the Implementation of Primitive Visual Cortex Model in Retina Using Gabor Wavelet (가버 웨이블릿을 이용한 원시 시각 피질 모델 구현에 관한 연구)

  • Lee, Youngseok
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.13 no.6
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    • pp.477-482
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    • 2020
  • The human visual cortex has the characteristic that reacts sensitively to stimuli with special directional or temporal frequency changes while it is insensitive to selective stimuli of spatial phases. In this paper we implemented the model of complex cell using an image estimation iterative algorithm by Gabor wavelet transform. The performance of implemented model evaluated the consistency between the physiological experimental results in related papers. The implemented model is limited in the complete model of the receptive field in the retina where simple cells and complex cells are distributed together. But the implemented model express the reaction of the complex cells from the point of view of the detection of corners and edges.

Artificial Vision System using Human Visual Information Processing (시각정보처리과정을 이용한 인공시각시스템)

  • Seo, Chang-Jin
    • Journal of Digital Convergence
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    • v.12 no.11
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    • pp.349-355
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    • 2014
  • In this paper, we propose the artificial vision system using human visual information processing and wavelet. Artificial vision system may be used for the visually impaired person and the machine recognition system. In this paper, we have constructed the information compression process to ganglion cells from the human retina. And we have reconstructed the primary visual information using recovery process to primary visual cortex from ganglion. Primary visual information is constructed by wavelet transformation using a high frequency and low frequency response. In the experiment, we used the faces database of AT&T. And the proposed method was able to improve the accuracy of face recognition considerably. And it was verified through experiments.

Single Cell Gel Electrophoresis (comet assay) to Detect DNA Damage and Apoptosis in Cell Level (DNA damage와 Apoptosis를 정량화하는 단세포전기영동법)

  • 류재천;김현주;서영록;김경란
    • Environmental Mutagens and Carcinogens
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    • v.17 no.2
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    • pp.71-77
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    • 1997
  • The single cell gel electrophoressis(SCGE) assay, also known as the comet assay, is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakage in mammalian cells. The SCGE or comet assay is a promising test for the detection of DNA damage and repair in individnal cells. It has widespread potential applications in DNA damage and repair studies, genotoxicity testing and biomonitoring. In this microgel electrophoresis technique, cells are embedded in agarose gel on microscope slides, iysed and electrophoresed under alkaline conditions. Cells with increased DNA damage display increased migration of DNA from the nucleus towards the anode. The length of DNA migration indicates the amount of DNA breakage in the cell. The comet assay is also capable of identifying apoptotic cells which contain highly fragmented DNA. Here we review the development of the SCGE assay, existing protocols for the detection and analysis of comets, the relevant underlying principles determining the behaviour of DNA and the potential applications of the technique.

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Analysis of Neuronal Activities of Retinal Ganglion Cells of Degenerated Retina Evoked by Electrical Pulse Stimulation (전기자극펄스에 대한 변성망막 신경절세포의 응답특성 분석)

  • Ryu, Sang-Baek;Lee, Jong-Seung;Ye, Jang-Hee;Goo, Yong-Sook;Kim, Chi-Hyun;Kim, Kyung-Hwan
    • Journal of Biomedical Engineering Research
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    • v.30 no.4
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    • pp.347-354
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    • 2009
  • For the reliable transmission of meaningful visual information using prosthetic electrical stimulation, it is required to develop an effective stimulation strategy for the generation of electrical pulse trains based on input visual information. The characteristics of neuronal activities of retinal ganglion cells (RGCs) evoked by electrical stimulation should be understood for this purpose. In this study, for the development of an optimal stimulation strategy for visual prosthesis, we analyzed the neuronal responses of RGCs in rd1 mouse, photoreceptor-degenerated retina of animal model of retinal diseases (retinitis pigmentosa). Based on the in-vitro model of epiretinal prosthesis which consists of planar multielectrode array (MEA) and retinal patch, we recorded and analyzed multiunit RGC activities evoked by amplitude-modulated electrical pulse trains. Two modes of responses were observed. Short-latency responses occurring at 3 ms after the stimulation were estimated to be from direct stimulation of RGCs. Long-latency responses were also observed mainly at 2 - 100 ms after stimulation and showed rhythmic firing with same frequency as the oscillatory background field potential. The long-latency responses could be modulated by pulse amplitude and duration. From the results, we expect that optimal stimulation conditions such as pulse amplitude and pulse duration can be determined for the successful transmission of visual information by electrical stimulation.

Electrically-evoked Neural Activities of rd1 Mice Retinal Ganglion Cells by Repetitive Pulse Stimulation

  • Ryu, Sang-Baek;Ye, Jang-Hee;Lee, Jong-Seung;Goo, Yong-Sook;Kim, Chi-Hyun;Kim, Kyung-Hwan
    • The Korean Journal of Physiology and Pharmacology
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    • v.13 no.6
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    • pp.443-448
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    • 2009
  • For successful visual perception by visual prosthesis using electrical stimulation, it is essential to develop an effective stimulation strategy based on understanding of retinal ganglion cell (RGC) responses to electrical stimulation. We studied RGC responses to repetitive electrical stimulation pulses to develop a stimulation strategy using stimulation pulse frequency modulation. Retinal patches of photoreceptor-degenerated retinas from rd1 mice were attached to a planar multi-electrode array (MEA) and RGC spike trains responding to electrical stimulation pulse trains with various pulse frequencies were observed. RGC responses were strongly dependent on inter-pulse interval when it was varied from 500 to 10 ms. Although the evoked spikes were suppressed with increasing pulse rate, the number of evoked spikes were >60% of the maximal responses when the inter-pulse intervals exceeded 100 ms. Based on this, we investigated the modulation of evoked RGC firing rates while increasing the pulse frequency from 1 to 10 pulses per second (or Hz) to deduce the optimal pulse frequency range for modulation of RGC response strength. RGC response strength monotonically and linearly increased within the stimulation frequency of 1~9 Hz. The results suggest that the evoked neural activities of RGCs in degenerated retina can be reliably controlled by pulse frequency modulation, and may be used as a stimulation strategy for visual neural prosthesis.