• Title/Summary/Keyword: viscous

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Establishment of a quality index of surfactin production during cheonggukjang fermentation (청국장 발효 중 surfactin 생산량 품질지표 설정)

  • Chang-Geun Ji;Jeong-Hwa Hong;Sang-Hyun Lee
    • Food Science and Preservation
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    • v.30 no.2
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    • pp.311-320
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    • 2023
  • Surfactin, one of the most powerful biosurfactants, can be widely applied in agriculture, food, and pharmaceutics. The purpose of the present study was to establish suitable indicators for a rapid detection method that can confirm the surfactin productivity of cheonggukjang. In the present study, changes in the total number of bacteria were quantified according to the fermentation time of chenggukjang. Furthermore, physicochemical factors, such as pH, color value, surface tension, refractive index, absorbance, and protein contents, were assessed in chenggukjang viscous substances, and a correlation analysis between the physicochemical factors and surfactin contents was performed. We found that the protein contents gradually increased up to 48 h (4.21±0.11 mg/mL) without a change in the surface tension. Furthermore, the refractive index, absorbance at 280 nm, and color value were significantly increased as the fermentation time increased; however, there were no statistically significant differences after 36 h of fermentation. Interestingly, this result exhibited a tendency similar to the surfactin content according to fermentation time. These findings suggest that surfactin can be used as a suitable quality indicator and may provide an experimental basis for the fermented food industry.

Fluid bounding effect on FG cylindrical shell using Hankel's functions of second kind

  • Khaled Mohamed Khedher;Shahzad Ali Chattah;Mohammad Amien Khadimallah;Ikram Ahmad;Muzamal Hussain;Rana Muhammad Akram Muntazir;Mohamed Abdelaziz Salem;Ghulam Murtaza;Faisal Al-Thobiani;Muhammad Naeem Mohsin;Abeera Talib;Abdelouahed Tounsi
    • Advances in nano research
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    • v.16 no.6
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    • pp.565-577
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    • 2024
  • Vibration investigation of fluid-filled functionally graded cylindrical shells with ring supports is studied here. Shell motion equations are framed first order shell theory due to Sander. These equations are partial differential equations which are usually solved by approximate technique. Robust and efficient techniques are favored to get precise results. Employment of the Rayleigh-Ritz procedure gives birth to the shell frequency equation. Use of acoustic wave equation is done to incorporate the sound pressure produced in a fluid. Hankel's functions of second kind designate the fluid influence. Mathematically the integral form of the Langrange energy functional is converted into a set of three partial differential equations. A cylindrical shell is immersed in a fluid which is a non-viscous one. These shells are stiffened by rings in the tangential direction. For isotropic materials, the physical properties are same everywhere where the laminated and functionally graded materials, they vary from point to point. Here the shell material has been taken as functionally graded material. After these, ring supports are located at various positions along the axial direction round the shell circumferential direction. The influence of the ring supports is investigated at various positions. Effect of ring supports with empty and fluid-filled shell is presented using the Rayleigh - Ritz method with simply supported condition. The frequency behavior is investigated with empty and fluid-filled cylindrical shell with ring supports versus circumferential wave number and axial wave number. Also the variations have been plotted against the locations of ring supports for length-to-radius and height-to-radius ratio. Moreover, frequency pattern is found for the various position of ring supports for empty and fluid-filled cylindrical shell. The frequency first increases and gain maximum value in the midway of the shell length and then lowers down. It is found that due to inducting the fluid term frequency result down than that of empty cylinder. It is also exhibited that the effect of frequencies is investigated by varying the surfaces with stainless steel and nickel as a constituent material. To generate the fundamental natural frequencies and for better accuracy and effectiveness, the computer software MATLAB is used.

Steady Shear Flow and Dynamic Viscoelastic Properties of Semi-Solid Food Materials (반고형 식품류의 정상유동특성 및 동적 점탄성)

  • 송기원;장갑식
    • The Korean Journal of Rheology
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    • v.11 no.2
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    • pp.143-152
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    • 1999
  • Using a Rheometrics Fluids Spectrometer(RFS II), the steady shear flow and the small-amplitude dynamic viscoelastic properties of three kinds of semi-solid food materials(mayonnaise, tomato ketchup, and wasabi) have been measured over a wide range of shear rates and angular frequencies. The shear rate dependence of steady flow behavior and the angular frequency dependence of dynamic viscoelastic behavior were reported from the experimentally measured data. In addition, some viscoplastic flow models with a yield stress term were employed to make a quantitative evaluation of the steady flow behavior, and the applicability of these models was also examined in detail. Furthermore, the correlations between steady shear flow(nonlinear behavior) and dynamic viscoelastic(linear behavior)properties were discussed using the modified power-law flow equations. Main results obtained from this study can be summarized as follows : (1) Semi-solid food materials are regarded as viscoplastic fluids having a finite magnitude of yield stress, and their flow behavior shows shear-thinning characteristics, exhibiting a decrease in steady flow viscosity with increasing shear rate. (2) The Herschel-Bulkley, Mizrahi-Berk, and Heinz-Casson models are all applicable to describe the steady flow behavior of semi-solid food materials. Among these models, the Heinz-Casson model has the best validity. (3) Semi-solid food materials show a stronger shear-thinning behavior at shear rate region higher than a critical shear rate where a more progressive structure breakdown takes place. (4) Both the storage and loss moduli are increased with increasing angular frequency, but they have a slight dependence on angular frequency. The elastic behavior is dominant to the viscous behavior over a wide range of angular frequencies. (5) All of the steady flow, dynamic, and complex viscosities are well satisfied with the power-law model behavior. The relationships between steady shear flow and dynamic viscoelastic properties can well be described by the modified forms of the power-law flow equations.

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The Characteristics of Longitudinal Permeability and Hydraulic Resistance in Stem of Acer mono (고로쇠나무 줄기의 통수성(通水性)과 통수저항(通水抵抗)의 특성(特性))

  • Kim, Sun-Hee;Han, Sang-Sup
    • Journal of Korean Society of Forest Science
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    • v.89 no.4
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    • pp.488-496
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    • 2000
  • This study was carried out to investigate the characteristics of longitudinal permeability and hydraulic resistance, and to compare the longitudinal permeability ($K_E$) calculated by the Hagen-Poiseuille's law (Siau, 1971) and the longitudinal permeability (K) measured in sapwood of Acer mono stem. The volume flow rate (Q) in a vessel was $0.80{\times}10^{-4}cm^3/sec$ and the hydraulic resistance ($R_S$) to viscous flow through a vessel was, on average, $1.37{\times}10^{10}dyn{\cdot}sec{\cdot}cm^{-3}{\cdot}cm^{-2}$. The average value of volume flow rate ($Q_N$) through the cross section of sapwood was $0.32cm^3{\cdot}sec^{-1}{\cdot}cm^{-2}$, and the average resistance ($R_{SN}$) was $3.59{\times}10^6dyn{\cdot}sec{\cdot}cm^{-3}{\cdot}cm^{-2}$. The values of K decreased as the diameter of stem increases, which was attributable to variations in the number of vessel per unit area rather than in vessel diameter, and to different resistances in the conducting tissues of each part of the stem. The average value of K measured at breast height was 31% of average value of $K_E$. The $K/K_E$ ratios were 100% in 4 to 6 year-old stems and more than 90% in 7 to 27 year-old stems. The $K/K_E$ ratio decreased as the age of stems increases, and was not more than 20% in near-ground parts of stem.

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Production and Characterization of Extracellular Polysaccharide Produced by Pseudomonas sp. GP32 (Pseudomonas sp. GP32에 의해 생산된 세포 외 다당류의 생산 및 특성)

  • Lee, Myoung Eun;Lee, Hyun Don;Suh, Hyun-Hyo
    • Journal of Life Science
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    • v.25 no.9
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    • pp.1027-1035
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    • 2015
  • A strain GP32 which produces a highly viscous extracellular polysaccharide was conducted with soil samples and identified as Pseudomonas species. The culture flask conditions for the production of extracellular polysaccharide by Pseudomonas sp. GP32 were investigated. The most suitable carbon and nitrogen source for extracellular polysaccharide production were galactose and (NH4)2SO4. The optimum carbon/nitrogen ratio for the production of extracellular polysaccharide was around 50. The optimum pH and temperature for extracellular polysaccharide production was 7.5 and 32℃, respectively. In batch fermentation using a jar fermentor, the highest extracellular polysaccharide content (15.7 g/l) was obtained after 70 hr of cultivation. The extracellular polysaccharide produced by Pseudomonas sp. GP32 (designated Biopol32) was purified by ethanol precipitation, cetylpyridinium chloride (CPC) precipitation, and gel permeation chromatography. Biopol32, which has an estimated molecular weight of over 3×107 datons, is a novel polysaccharide derived from sugar components consisting of galactose, glucose, gulcouronic acid and galactouronic acid in an approximate molar ratio of 1.85 : 3.24 : 1.00 : 1.42. The solution of Biopol32 showed non-Newtonian characteristics. The viscosity of Biopol32 exhibited appeared to be higher at all concentration compared to that of zooglan from Zoogloea ramigera. An analysis of the flocculating efficiency of Biopol32 in industry wastewater (food, textile, and paper wastewater) revealed chemical oxygen demand (COD) reduction rates 58.4-67.3% and suspended solid (SS) removal rates 82.6-91.3%. Based on these results, Biopol32 is a possible candidate for industrial applications such as wastewater treatment.

The Formation of the Cenozoic Volcanic Edifice in the Goseong-Ganseong Area, Gangwondo, Korea (강원도 고성-간성일대의 신생대 화산체의 형성과정)

  • Kim, Hwa Sung;Kil, Youngwoo;Lee, Moon Won
    • Journal of the Korean earth science society
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    • v.33 no.7
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    • pp.627-636
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    • 2012
  • In the Obongri-Goseong area of Gangwondo, South Korea, there are six densely distributed volcanic edifices i.e., Duibaejae, Oeumsan, Galmibong, 249 m height, 166 m height, and 102 m height, and two other volcanic edifices including Goseongsan and Unbongsan volcanic edifice that are separately located from a distance. A previously undiscovered 249m volcanic edifice in Obongri was found in this investigation, and the six volcanic edifices distributed in Obongri will be referred to as the Obongri volcanic edifice group. Volcanic edifices in this area were interpreted by other researchers as being volcanic plug, plug dome, and cylindrical volcanic pipe type edifices. The aim of this study is to investigate the aspect of volcanic activity in the Obongri-Goseong area and the formation of volcanic edifices by examining of the shape of volcanic edifices, stratigraphy, and characterization of volcanic products. All the volcanic edifices in the area are composed of basaltic rocks on the Mesozoic granite basement, and the prevalence of the dome shape increased towards the upper part of the mountain. Three volcanic edifices (Duibaejae, 166 m height, 102 m height) include intercalated pyroclastic deposits between the basaltic rocks and the basement. The pyroclastic deposit in the Duibaejae volcanic edifice is composed of quartz, feldspar, granite fragments originated from the basement, and scoria fragments originated from the volcanic eruption. In addition to angular olivine, plagioclase, and pyroxene xenocrysts, all the basaltic rocks contained mantle xenolith, gabbroic xenolith originated from the lower crust, and granitic xenolith originated from the basement. This fact indicates that magma rapidly rose to the surface and that the volcanic activity was explosive. It is also interpreted that, as the basaltic magma became highly viscous due to the large amount of xenocrysts, the erupted magma formed a dome structure on the surface. The original dome structure was then severely eroded out leaving a plug dome formation on the basement.

Characteristics of Edible Films Based with Various Cultivars of Sweet Potato Starch (고구마 전분을 이용한 가식성 필름의 제조와 특성)

  • Lee, Jung-Ju;Rhim, Jong-Whan
    • Korean Journal of Food Science and Technology
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    • v.32 no.4
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    • pp.834-842
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    • 2000
  • In order to investigate the characteristics of various sweet potato starches, gelatinization temperatures, solution viscosity of starch separated from two cultivars of the dry type sweet potatoes(Yulmi and Gunmi), one cultivar of moist type sweet potato(Jinmi), and one cultivar of purple colored variety(Jami) were compared, and properties of the edible films prepared with the starches were determined. Under a differential scanning colorimetry(DSC), initial temperatures for starch gelatinization of the dry type sweet potatoes (Yulmi and Gunmi) were higher than that of the moist type sweet potato (Jinmi), and that of Jami was close to those of the dry type ones. The sweet potato starch solutions tested by a cone and plate viscometer, showed peudoplastic characteristics. The moist type sweet potato was the most viscous followed by Jami, Yulmi, and Gunmi among the tested starch solutions. Total color difference of the edible films prepared with different cultivars of sweet potatoes showed appreciable differences between cultivars, caused by differences in Hunterb values. Water Vapor Permeability (WVP) of sweet potato starch films also showed significant differences between cultivars. Films prepared with the dry type sweet potato, Gunmi, showed the lowest WVP value of $0.83{\times}10^{-9}\;g\;{\cdot}\;m/m^{2}\;{\cdot}\;s\;{\cdot}\;Pa$, followed by Jami, Yulmi, and Jinmi. Water solubility of the films did not show any significant differences between cultivars. Tensile strength of the dry type sweet potato and Jami, which ranged 14.18-18.75 MPa, were higher than that of the moist type sweet potato, which was 4.66 MPa. Elongation values of the films, which were 5-6%, indicated that sweet potato starch films were not so elastic.

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Effectiveness and Preparation of Nano-emulsion of a Rapeseed Oil Extract Originated from Jeju with PIT Emulsifying System (PIT유화시스템을 이용한 제주산 유채씨앗 오일추출물의 나노에멀젼의 제조 및 효과)

  • Joo, Se-Jin;Kim, Hack-Soo;Lee, Jeong-Koo;Lee, Min-Hee;Kim, In-Young
    • Journal of the Korean Applied Science and Technology
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    • v.29 no.3
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    • pp.486-494
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    • 2012
  • Nano-emulsion with phase inversion temperature (PIT) emulsifying system was prepared to use rapeseed oil from originating Jeju in order to apply various cosmetic applications. Natural rape seed oil (NRSO) extraction was extracted using n-hexane as a solvent. NRSO extract showed a light yellowish color of viscous liquid as well as yield was $43{\pm}2.5%$. Acid value was $2.76{\pm}0.5$ and gravity was $0.89{\pm}0.05$. Droplet size of PIT-Yuche-NE with 20wt% of rapeseed oil was 50-120nm (average: $82{\pm}5.8nm$) and zeta potential was -29.5mV. It was thermodynamically good stable emulsion due to $(PEG)_{5-30}$fattyacidether. Some conclusions from the result of characteristic experiment were obtained as follows. First, the anti-oxidative activity was measured by free radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl radical). Anti-oxidative activity of PIT-Yuche-NE was $37.2{\pm}6.7%$ on 10mg/mL compared with PIT-Toco-NE (Natural tocopherol nano-emulsion, $28.8{\pm}6.5%$ on 10 mg/mL) and PIT-Nokcha-NE (Green tea extract nano-emulsion, $29.6{\pm}7.2%$ on 10mg/mL). Second, the collagen synthesis activity of PIT-Yuche-NE was $148{\pm}15.2%$ compared with PIT-Toco-NE (Natural tocopherol nano-emulsion, $121{\pm}13.5%$ on 10mg/mL) and PIT-Nokcha-NE (Green tea extract nano-emulsion, $95{\pm}12.7%$ on 10mg/mL). Third, the effectiveness of moisturizing activity of Yuche-CRM with Aramo-TS after 6 hours increase $47{\pm}3.9%$ (*p-value£0.05, n=7) whereas Both Toco-CRM was $30{\pm}5.2%$ (*p-value£0.05, n=7) and Nokcha-CRM was $35{\pm}4.5%$. Therefore, Yuche-CRM has higher moisturizing effect than other two creams. Finally, Nano-emulsion stabilizing rapeseed oil using PIT emulsifying system of this study can be used to apply cosmetics industry and pharmaceutical industry.

EFFECT OF A NEW RESIN MONOMER ON THE MICROLEAKAGE OF COMPOSITE RESIN RESTORATIONS (새로운 레진 단량체가 복합레진수복물의 미세변연누출에 미치는 영향)

  • Bae, J.H.;Kim, Y.K.;Yoon, P.Y.;Lee, M.A.;Cho, B.H.
    • Restorative Dentistry and Endodontics
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    • v.32 no.5
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    • pp.469-475
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    • 2007
  • The purpose of this study was to evaluate the effect of a new resin monomer on the microleakage of composite resin restorations. By adding new methoxylated Bis-GMA (Bis-M-GMA, 2,2-bis[4-(2-methoxy-3-methacryloyloxy propoxy) phenyl] propane) having low viscosity, the content of TEGDMA which has adverse effects on polymerization shrinkage might be decreased. As a result, microleakage might be improved. $2\;mm\;{\times}\;2\;mm\;{\times}\;2\;mm$ cavities with occlusal margins in enamel and gingival margins in dentin were prepared on buccal and lingual surfaces of 40 extracted human premolars. Prepared teeth were randomly divided into four groups and restored with Clearfil SE bond (Kuraray, Japan) and one of experimental composite resins; EX1, Experimental composite resin1 (Bis-M-GMA/TEGDMA = 95/5 wt%, 40 mm nanofillers); EX2, Experimental composite resin2 (Bis-M-GMA/TEGDMA = 95/5 wt%, 20 mm nanofillers); EX3, Experimental composite resin3 (Bis-GMA/TEGDMA = 70/30 wt%, 40 nm nanofillers); and Filtek Z250 (3M ESPE, USA) was filed as a control group. The restored teeth were thermocycled, and immersed in 2% methylene blue solution for 24 hours. The teeth were sectioned buccolingually with a low speed diamond saw and evaluated for microleakage under stereomicroscope. The data were statistically analyzed by Pearson Chi-Square test and Fisher Exact test (p = 0.05). The microleakage scores seen at the enamel margin were significantly lower than those of dentin margin (p = 0.007). There were no significant differences among the composite resins in the microleakage scores within each margin (p > 0.05). Bis-M-GMA, a new resin monomer having low viscosity, might in part replace high viscous Bis-CMA and might improve the quality of composite resin.

Strain Improvement and Bioprocess Optimization for Enhanced Production of Haluronic Acid(HA) in Bioreactor Cultures of Streptococcus zooepidemicus (히알루론산 생산성 향상을 위한 Streptococcus zooepidemicus 균주 개량 및 발효조 배양공정 최적화)

  • Kim, Soo Yeon;Chun, Gie-Taek
    • Microbiology and Biotechnology Letters
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    • v.48 no.3
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    • pp.344-357
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    • 2020
  • Strain improvement and bioprocess development were undertaken to enhance hyaluronic acid(HA) production by Streptococcus zooepidemicus cells. Using a high-yielding mutant strain, statistical medium optimization was carried out in shake flask cultures, resulting in 52% increase in HA production (5.38 g/l) at the optimal medium composition relative to the parallel control cultures. For sufficient supply of dissolved oxygen (DO), which turned out to be crucial for enhanced production of HA, agitation system and speed were intensively investigated in 5 L bioreactor cultures. Increase in oxygen mass transfer coefficient (kLa) through increment of agitation speed (rpm) and 35% expansion of diameter of the newly-designed impellers showed significantly positive effects on HA production. By installing an expanded Rushton-turbine impeller for efficient break-down of sparged air, and an extended marine impeller above the Rushton-turbine impeller for efficient mixing of the air-born viscous fermentation broth, maximum amount of HA (9.79 g/l) was obtained at 450 rpm, 1.8 times higher level than that of the corresponding flask culture. Subsequently, the possibility of bioprocess scale-up to a 50 L bioreactor was investigated. Despite almost identical maximum HA production (9.11 vs 9.25 g/l), the average HA volumetric productivity (rp) of the 50 L culture turned out only 74% compared to the corresponding 5 L culture during the exponential phase, possibly caused by shear damages imposed on the producing cells at the high stirring in the 50 L culture. The scale-up process could be successfully achieved if a scale-up criterion of constant oxygen mass transfer coefficient (kLa) is applied to the 50 L pilot-scale bioreactor system.