• 제목/요약/키워드: virus spread

검색결과 327건 처리시간 0.025초

COVID-19 Fake News Detection with Deep Learning

  • Rutchaneewan Kowirat;Laor Boongasame
    • Asia pacific journal of information systems
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    • 제33권1호
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    • pp.69-82
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    • 2023
  • Social media has become one of the most popular channels to keep updated with daily news because it can quickly and easily access information. This advantage is used by malicious people to spread fake news widely. Since the COVID-19 pandemic, fake news has become a huge social problem, causing people to panic and misunderstand how to cure or protect themselves from the virus. So, the goal of this research is to use deep learning as the Recurrent Neural Network (RNN) model to find fake news about COVID-19 in the Thai language on social media and help filter information by classifying real and fake news.

Detection of Viral Hemorrhagic Septicemia Virus (VHSV) from marine fish in the South Western Coastal Area and East China Sea (남.서해안과 동중국해 자연산 어류에서 Viral Hemorrhagic Septicemia Virus(VHSV)검출)

  • Lee, Wol-La;Yun, Hyun-Mi;Kim, Seok-Ryel;Jung, Sung-Ju;Oh, Myung-Joo
    • Journal of fish pathology
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    • 제20권3호
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    • pp.201-209
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    • 2007
  • Viral hemorrhagic septicemia (VHS) is one of the most serious viral disease of farmed rainbow trout and some marine fishes in Europe and North America. It has been reported in various marine fish species of Asian countries and induced cause mass mortality in Japanese flounder (Paralichthys olivaceus) culturing in Korea. The aims of this study were to monitor VHSV in wild marine fishes and to give critical information for controling the disease through prophylactic methods. Prevalence of the viral disease, geological distribution and reservoir of the virus were investigated using wild marine fishes captured in southern coast and east china sea for two years. (Reverse Transcriptase Polymerase Chain Reaction) RT-PCR results showed that VHSV were detected in 17 (10.6%) out of 160 fish. G gene sequences of viral strains isolated in this study were closely related to that of a reference strain, KVHS01-1, belonging to VHSV genotype Ⅰ. The results suggest that some of wild marine fishes are VHSV carriers and may spread the pathogen directly to fish farmed in coastal area.

Ebola Hemorrhagic Fever Outbreaks: Diagnosis for Effective Epidemic Disease Management and Control (에볼라 출혈열 발병 : 효과적인 전염병 관리 및 통제를 위한 진단)

  • Kang, Boram;Kim, Hyojin;Macoy, Donah Mary;Kim, Min Gab
    • Microbiology and Biotechnology Letters
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    • 제45권2호
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    • pp.87-92
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    • 2017
  • The first Ebola hemorrhagic fever outbreak occurred in the Democratic Republic of Congo and Sudan in 1976 and then emerged in West Africa in 2014 with a total of 27,741 cases and 11,284 deaths. The fever is caused by the Ebola virus, which belongs to the Filoviridae family and contains a ssRNA genome. The known subtypes of the virus are Bundibugyo ebolavirus, Reston ebolavirus, Sudan ebolavirus, $Ta\ddot{i}$ Forest ebolavirus, and Zaire ebolavirus. The Ebola outbreak was historically originated majorly from the East and Central African tropical belt. The current outbreaks in West Africa caused numerous deaths and spread fear in global society. In the absence of effective treatment strategies and any vaccine, accurate diagnosis is the most important contributing factor in the management and control of the epidemic disease. WHO (World Health Organization) has announced emergency guidance for the selection and use of Ebola in in vitro diagnostic assays. Numerous companies and research institutions have studied the various diagnosis methods and identified four WHO procurement approved as diagnosis kits: RealStar Ebolavirus Screen RT-PCR kit 1.0 (Altona), Liferiver-Ebola Virus (EBOV) Real time RT-PCR kit, Xpert Ebola Assay, and ReEBOV Antigen Rapid Test Kit. The efficiency of novel diagnostic kits such as Rapid Diagnosis Test (RDT) is currently being evaluated.

Antiviral Efficacy of Citra-kill®, Disinfectant Solution Against Avian Influenza Virus

  • Cha, Chun-Nam;Lee, Yeo-Eun;Kang, In-Jin;Yoo, Chang-Yeul;Park, Eun-Kee;An, Sun-Jeong;Kim, Suk;Lee, Hu-Jang
    • Journal of Food Hygiene and Safety
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    • 제27권1호
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    • pp.18-23
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    • 2012
  • Highly pathogenic avian influenza virus (HPAIV) is already panzootic in poultry and caused a considerable economic loss in poultry industry. In addition, HPAIV continues to cross species barriers to infect humans and other mammals, often with fatal outcomes. In this study, the virucidal efficacy of Citra-$Kill^{(R)}$ composed to quaternary ammonium chloride and citric acid was investigated against avian influenza H9N2 virus (AIV). A virucidal efficacy was determined with the viability of AIV contacted with the disinfectant in the allantoic membrane of chicken embryos. Citra-$Kill^{(R)}$ and AIV was reacted on the distilled water (DW), hard water (HW) or organic matter suspension (OM) condition. On DW condition, AIV was inactivated with 2,000 fold dilutions of Citra-$Kill^{(R)}$. When the antiviral effect on HW condition was evaluated, the antiviral activity of the disinfectant showed on 1,500 fold dilutions against AIV. With the investigation of the antiviral effect of the disinfectant on OM condition, AIV was inactivated on 500 fold dilutions of Citra-$Kill^{(R)}$. As Citra-$Kill^{(R)}$ possesses virucidal efficacy against AIV, the disinfectant solution can be used to limit the spread of animal viral diseases.

Rapid Determining for Subtypes and Pandemic Type of Swine Influenza Virus by Diagnostic One-step RT-PCR (진단용 one-step RT-PCR을 통한 돼지 인플루엔자 바이러스의 아형 및 pandemic 유형에 대 한 신속한 결정)

  • Kim, Gwang Il;Kim, Jee In;Kwon, Jin-Hyeap;Min, Yoo Hong;Kang, Joo Il;Lee, Chang-Ho;Kim, Sung-Hee;Lim, Jae-Hwan
    • Journal of Life Science
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    • 제28권5호
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    • pp.555-562
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    • 2018
  • Swine influenza virus (SIV) causes one of the most common diseases of the pig population, and its subtypes are determined by hemagglutinin (HA) and neuraminidase (NA). Recently, the SIV subtype diagnosis has been developed. The method using antigen-antibody reaction rather than PCR was mainly used because of the large change in the ribonucleotide sequences of SIV. Here, we have developed 10 diagnostic primer sets through multi-nucleotide sequences alignment of spreaded SIV since 2008 in Korea and then optimized the reaction of the one-step RT-PCR for rapid determination of SIV subtype. In addition, specific primers were designed to early determine the pandemic SIV by detecting unique M sequences proven in highly infectious and virulent subtypes of the influenza H1N1 (pH1N1). Here, some of the SIVs spread in Korea from 2008 to 2014 have been tested to determine the subtypes and pandemic potential of SIV. All diagnostic primer sets were found to be able to accurately determine the SIV subtype and to detect the pandemic SIV. In conclusion, it was confirmed that the optimized one-step RT-PCR analysis using these primer sets is useful for rapid diagnosis of SIV subtypes. These results can be used for development of SIV subtype diagnostic kit to early detect before virulent SIV spreads do.

Monitoring and Distribution of Mosquitoes in the Downtown Areas and Migratory Bird Habitats in the Jeonbuk Region, 2021 (2021년 전북지역 도심과 철새서식처의 모기 감시와 분포)

  • Park, Ho;Kwon, Pil-Seung
    • Korean Journal of Clinical Laboratory Science
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    • 제54권2호
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    • pp.142-148
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    • 2022
  • This survey was performed to monitor the spread of specific mosquito-borne pathogens at Jeonbuk. The frequency of occurrence of mosquito borne pathogens including Japanese encephalitis virus, West Nile virus, Zika virus, and yellow fever virus was assessed by collecting mosquitoes twice a month from March to December 2021 from various areas in Jeonbuk. A total of 15,975 mosquitoes from 15 species and 7 genera were collected. The highest number of 9,116 mosquitoes (trap index: TI, 506.4) were collected in the Wanju cattle pen, followed by the habitat for migratory birds and the downtown area in Jeonju. In the Gunsan habitat for migratory birds, 3,217 mosquitoes (TI, 178.7) were collected in the reed fields, 356 (TI, 19.7) in the men's toilets, and 1,948 (TI, 108.2) in the women's toilets. In Jeonju, 677 mosquitoes (TI, 37.6) were collected in the Deokjin park, 358 (TI, 19.8) in the Deokjin-gu office, and 303 (TI, 16.8) at the Jeonbuk National University. The largest population of mosquitoes was collected in the men's toilets in Gunsan and the Deokjin Park in downtown Jeonju. The results of the RT-PCR confirmation to determine the pathogen infection of the collected mosquitoes were all negative. These results provide a basis for tackling integrated mosquito-borne diseases in the Jeonbuk region.

Identification of a conservative site in the African swine fever virus p54 protein and its preliminary application in a serological assay

  • Xu, Lingyu;Cao, Chenfu;Yang, Zhiyi;Jia, Weixin
    • Journal of Veterinary Science
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    • 제23권4호
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    • pp.55.1-55.12
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    • 2022
  • Background: ASF was first reported in Kenya in 1910 in 1921. In China, ASF spread to 31 provinces including Henan and Jiangsu within six months after it was first reported on August 3, 2018. The epidemic almost affected the whole China, causing direct economic losses of tens of billions of yuan. Cause great loss to our pig industry. As ELISA is cheap and easy to operate, OIE regards it as the preferred serological method for ASF detection. P54 protein has good antigenicity and is an ideal antigen for detection. Objective: To identify a conservative site in the African swine fever virus (ASFV) p54 protein and perform a Cloth-enzyme-linked immunosorbent assay (ELISA) for detecting the ASFV antibody in order to reduce risks posed by using the live virus in diagnostic assays. Method: We used bioinformatics methods to predict the antigen epitope of the ASFV p54 protein in combination with the antigenic index and artificially synthesized the predicted antigen epitope peptides. Using ASFV-positive serum and specific monoclonal antibodies (mAbs), we performed indirect ELISA and blocking ELISA to verify the immunological properties of the predicted epitope polypeptide. Results: The results of our prediction revealed that the possible antigen epitope regions were A23-29, A36-45, A72-94, A114-120, A124-130, and A137-150. The indirect ELISA showed that the peptides A23-29, A36-45, A72-94, A114-120, and A137-150 have good antigenicity. Moreover, the A36-45 polypeptide can react specifically with the mAb secreted by hybridoma cells, and its binding site contains a minimum number of essential amino acids in the sequence 37DIQFINPY44. Conclusions: Our study confirmed a conservative antigenic site in the ASFV p54 protein and its amino acid sequence. A competitive ELISA method for detecting ASFV antibodies was established based on recombinant p54 and matching mAb. Moreover, testing the protein sequence alignment verified that the method can theoretically detect antibodies produced by pigs affected by nearly all ASFVs worldwide.

Comparison of Blood Test Results and Symptoms of Patients with COVID-19 Monoinfection and with COVID-19 and Influenza Virus Co-Infection (COVID-19 단일 감염 환자와 COVID-19 및 인플루엔자 바이러스 동시 감염 환자의 혈액 검사 결과 및 증상 비교)

  • Jung, Bo Kyeung;Ham, Seung Keun;Kim, Jae Kyung
    • Korean Journal of Clinical Laboratory Science
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    • 제54권2호
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    • pp.103-109
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    • 2022
  • In December 2019, the coronavirus disease 2019 (COVID-19) caused by the virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in China and spread rapidly around the world, infecting millions of people. Cases of COVID-19 infection were observed to lead to viral pneumonia. Thirty-five patients admitted to the Gyeonggi Medical Center, South Korea, between November 2020 to January 2021, were found to have been infected with the influenza virus A and B, which cause symptoms similar to COVID-19. The records of these patients and those of COVID-19 patients who visited the hospital for medical examination were compared. The study patients included thirty patients with COVID-19 and/or influenza, five of those with influenza alone. A group of 121 patients without infection was used as control. Patients with COVID-19 and influenza had significantly higher lactate dehydrogenase levels than the patients with COVID-19 alone. The erythrocyte sedimentation rate (ESR) was higher in patients with COVID-19 alone than in other groups. Significant clinical outliers were observed in the COVID-19 and influenza infection group compared with the COVID-19 alone group. These results are expected to play an important role in the analysis of the hematological data of infected patients and the comparison of simultaneous and single infection data to determine clinical symptoms and other signs. These results may also assist in the development of vaccines and treatments for COVID-19.

Clinical characterization of 3-month-old pigs infected with African swine fever virus from Vietnam

  • Oh, Sang-Ik;Bui, Vuong Nghia;Dao, Duy Tung;Bui, Ngoc Anh;Yi, Seung-Won;Kim, Eunju;Lee, Han Gyu;Bok, Eun-Yeong;Wimalasena, S.H.M.P;Jung, Young-Hun;Hur, Tai-Young;Lee, Hu Suk
    • Korean Journal of Veterinary Service
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    • 제45권2호
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    • pp.71-77
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    • 2022
  • African swine fever (ASF) is a fatal viral disease in pigs, with a short incubation period and causing immediate death. Few studies exist on the Asian epidemic ASF virus (ASFV) challenge in older pigs, including growing and fattening pigs and sows. We aimed to investigate clinical outcomes, pathomorphological lesions, and viral distribution in organs of 3-month-old growing pigs that were inoculated with the ASFV isolated in Vietnam. The clinical outcomes were recorded daily, and the dead or euthanized pigs immediately underwent necropsy. Viral loads were determined in 10 major organs using quantitative polymerase chain reaction. The average incubation period in growing pigs was more delayed (5.2±0.9 dpi) than that in weaned pigs, and the clinical signs were milder in growing pigs than in weaned pigs. The digestive and respiratory clinical signs in growing pigs showed at the end period of life, but these were observed at an early stage of infection in weaned pigs. The pathomorphological features were severe and nonspecific with hemorrhagic lesions in various organs. The viral loads in organs from growing pigs were higher than those from piglets, and the number of viral copies was related to gross lesions in the tonsil and intestine. In the absence of vaccines against ASF, early clinical detection is important for preventing the spread of the virus. Our findings elucidated that the clinical signs and gross lesions in growing pigs differed from those in weaned pigs, which provide valuable information for diagnosis of pigs with suspected ASF infection.

An Automatic Access Registration System using Beacon and Deep Learning Technology (비콘과 딥러닝 기술을 활용한 전자출입명부 자동등록시스템)

  • Huh, Ji-Won;Ohm, Seong-Yong
    • The Journal of the Convergence on Culture Technology
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    • 제6권4호
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    • pp.807-812
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    • 2020
  • In order to prevent the national wide spread of the COVID-19 virus, the government enforces to use an electronic access registration system for public facilities to effectively track and manage the spread. Initially, there was a lot of hassle to write a directory, but recently a system for creating an electronic access list using QR codes, what is called KI-Pass, is mainly used. However, the procedure for generating a QR code is somewhat cumbersome. In this paper, we propose a new electronic access registration system that does not require QR code. This system effectively controls the suspicious visitor by using a mask wearing discriminator which has been implemented using deep learning technology, and a non-contact thermometer package. In addition, by linking the beacon, a short-range wireless communication technology, and the visitor's smartphone application, basic information of the facility visitor is automatically registered to KDCA through the server. On the other hand, the user access information registered in the server is encrypted and stored, and is automatically destroyed after up to 4 weeks. This system is expected to be very effective in preventing the spread of other new infectious diseases as well as responding to the coronavirus which is recording a high spread worldwide.