• The Korean Journal of Mycology
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• v.41 no.4
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• pp.261-267
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• 2013

In order to select antagonists for biological control of downy mildew of cucumber, 126 bacteria were isolated from cucumber plants collected from several locations in Korea. Among them, Five isolates were selected as potential biocontrol agents of cucumber downy mildew using a leaf disc bioassay method. In preventive and curative effect tests, the isolate CC110 was found to be effective to control downy mildew on cucumber showing diseased area by 0% whereas that of control was 15.0~18.0%. A bacterium isolate CC110 was identified as Bacillus amyloliquefaciens subsp. plantarum based on phylogenetic analysis using gyrB gene sequence. The culture liquid of isolate CC110 in TSB media were more effective for the control of the disease than those cultured in LB, NB, and KB media in leaf disk bioassay. when undiluted liquid, two-fold, five-fold diluted culture broth, and undiluted liquid, two-fold, five-fold diluted filtrate of isolate CC110 in TSB media were treated, diseased area of cucumber powdery mildew were 0%, 3.0%, 8.0%, 0%, 4.0% and 7.0%, respectively, whereas diseased area in the control was 21.0%. In the cucumber seedling tests, when the culture broth of isolate CC110 in TSB media was treated, diseased area were 35.0%, whereas that of control was 82.0%. When B. amyloliquefaciens CC110 was treated four times at five-day interval in the vinylhouse test, the control effect of cucumber downy mildew was higher than that treated three at seven-day interval.

• Korean Journal of Soil Science and Fertilizer
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• v.24 no.3
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• pp.192-199
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• 1991

To manufacture a good organic fertilizer through rapid composting process, cow dung was mixed with bulking materials such as rice straws, shredded bark, wood chips, and saw dust. The mixing ratio of the cow dung and bulking material was two to one on volume basis and moisture content was adjusted to 60 to 70 percent, C/N ratio 25 to 30 and aeration forced with suction during the aerated pile fermentation. Aerated pile was established outdoors and curing pile was in vinylhouse and the experiment was conducted for 3 years from '88 to '90. After pilling, temperature in aerated pile was reached to the maximum level at 7 to 9 days and the temperatures of cow dung + shredded bark, cow dung + wood chips, and cow dung + rice straws were higher than that of cow dung, and cow dung + saw dust. Total carbon, and C/N ratio in aerated pile fermentation period were increased in the middle stage and then they were decreased with the process of days, and nutrient contents in curing pile period had the same tendency as in the aerated pile.

• Korean Journal Plant Pathology
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• v.3 no.4
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• pp.252-260
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• 1987

Incidence of lettuce drop was observed throughout the growing season in the vinylhouse at the southern part of Korea, Kimhai. Occurrence of this disease was especially severe at the seedling stage. Number of sclerotia in surface soil $(30\times30\times5cm)$ was 22.0 at the seedling stage, and 5.3 at harvest in the infected area. Temperature for mycelial growth ranged from 5 to $30^{\circ}C$ with optimum temperature at $25^{\circ}C$. Sclerotia were formed fewer at low temperature, but their size was larger resulting in heavier dry weight than that at high temperature. The apothecia were formed from the sclerotia that were buried in March, April and September upto 3cm soil depth, but formed from those buried only 1 em soil depth in October. Sclerotia buried in June and December did not form apothecia regardless of soil depth by 90 days. The sclerotia buried in the 5cm of soil depth did not form apothecia. Sclerotia that were embedded in wet or flooded soil at $25^{\circ}C$ and $30^{\circ}C$ for 5 weeks lost their viability. Infection of lettuce was possible with mycelia originated from sclerotia on autoclaved lettuce plant fragments. The fungus was pathogenic on 25 plant species in 8 families in artificial inoculation tests. Lettuce seedlings appeared to be infected by airborne ascospore originated from sclerotia on crops and weeds around paddy fields, because sclerotia existing in soil might perish under long flood conditions during rice cultivation.

• Research in Plant Disease
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• v.19 no.2
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• pp.124-127
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• 2013

Black necrotic spots were observed from the fruits of paprika that were cultivating in a vinylhouse. The casual agents of the symptom were identified as several isolates of Pepper mild mottle virus (PMMoV) by responses of indicator plants, electron microscopy, and RT-PCR analysis. Symptoms of the viral disease were mild mottle in the young leaves, necrotic spots on the fruits and the fruit apex of paprika, but the symptoms were not shown on the mature leaves. All of the PMMoV isolates were determined as $P_{1.2.3}$ pathotypes from the biological responses on the chilli pepper lines used for discrimination of tobamovirus pathotypes. Pathogenicity of the PMMoV isolates was also confirmed using mechanical inoculation method to paprika seedlings. The coat protein (CP) genes of the PMMoV isolates were compared at the nucleotide and amino acid levels with the previously published PMMoV isolate. The isolates share 96 to 99% CP nucleotide identity among the isolates. The CP of $P_{1.2}$-pathotype PMMoV-P2 presented Met at position 139, But the CPs of $P_{1.2.3}$-pathotype PMMoVs from paprika showed Met to Asn substitution at the same position. This is the first report of identification of $P_{1.2.3}$-pathotype PMMoV isolates from paprika in Korea.

• Korean Journal of Weed Science
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• v.32 no.2
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• pp.115-120
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• 2012

Weed control is of fundamental importance when planting cereal crops, particularly during the establishment phase. Weeds compete for nutrients, water and light, and can severely threaten the survival and early growth of newly planted crops. Field experiment was conducted to evaluate the effect of ethanol treatment on the weed control efficacy in cereal crops in protected vinylhouse. Five treatments like water, 25 L, 50 L, 75 L, and 100 L per $m^{-2}$ at 2% ethanol were applied and covered with vinyl on 10 days before foxtail millet seeding. After that, foxtail millet seeds were sown on June 20 at $60{\times}10cm$ planting distance and weed control efficacy was recorded at 30, and 50 days after seeding based on the dry weight. Soil oxidation-reduction potential was recorded -200~-400 mV after treatment 2 days. Weed control efficacy of at 75 L, and 100 L per $m^{-2}$ was observed 92%, 84% in 2% ETOH, respectively. Yields of foxtail millet at 75 L, and 100 L per $m^{-2}$ were 1,570 kg $ha^{-1}$, 1,230 kg $ha^{-1}$, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.1
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• pp.27-31
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• 1985

To get well matured farm yard manure from ricestraw as quickly as possible during winter season, straw piles wrapped with polyethylene film and/or straw thatch were stored in the vinyl house or open air. Their maturities and changes of temperature in heap were investigated from the beginning of December 1983 to March of next year. Heat increment in vinyl house was high $2-5^{\circ}C$ than at open air at the lowest temperature but it didn't rise over the freezing point. However, the highest temperature was arisen over than $20^{\circ}C$ averagely at the vinylhouse compared to those of open air during three months. Temperature in piles of straw manure was reached to about $70^{\circ}C$ in maximum and rose again very rapidly after repiling in the vinyl house, whereas increment of temperature after repiling was delayed and took long times to reach the maximum temperature at open air. Wrapping with P.E. film also affected the insulation of decomposing heat of straw pile and promoted the repeat of piling even at open air. By these results, ricestraw would be decomposed rapidly by insulation with P.E. film in the vinyl house and could be reached to matured compost for application to field on next spring season. P.E. film covered for vinyl house was endured until May of next year without tear by weathering.