• Title/Summary/Keyword: viability monitoring

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Monitoring bridge scour using dissolved oxygen probes

  • Azhari, Faezeh;Scheel, Peter J.;Loh, Kenneth J.
    • Structural Monitoring and Maintenance
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    • v.2 no.2
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    • pp.145-164
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    • 2015
  • Bridge scour is the predominant cause of overwater bridge failures in North America and around the world. Several sensing systems have been developed over the years to detect the extent of scour so that preventative actions can be performed in a timely manner. These sensing systems have drawbacks, such as signal inaccuracy and discontinuity, installation difficulty, and high cost. Therefore, attempts to develop more efficient monitoring schemes continue. In this study, the viability of using optical dissolved oxygen (DO) probes for monitoring scour depths was explored. DO levels are very low in streambed sediments, as compared to the standard level of oxygen in flowing water. Therefore, scour depths can be determined by installing sensors to monitor DO levels at various depths along the buried length of a bridge pier or abutment. The measured DO is negligible when a sensor is buried but would increase significantly once scour occurs and exposes the sensor to flowing water. A set of experiments was conducted in which four dissolved oxygen probes were embedded at different soil depths in the vicinity of a mock bridge pier inside a laboratory flume simulating scour conditions. The results confirmed that DO levels jumped drastically when sensors became exposed during scour hole evolution, thereby providing discrete measurements of the maximum scour depth. Moreover, the DO probes could detect any subsequent refilling of the scour hole through the deposition of sediments. The effect of soil permeability on the sensing response time was also investigated.

Sensor enriched infrastructure system

  • Wang, Ming L.;Yim, Jinsuk
    • Smart Structures and Systems
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    • v.6 no.3
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    • pp.309-333
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    • 2010
  • Civil infrastructure, in both its construction and maintenance, represents the largest societal investment in this country, outside of the health care industry. Despite being the lifeline of US commerce, civil infrastructure has scarcely benefited from the latest sensor technological advances. Our future should focus on harnessing these technologies to enhance the robustness, longevity and economic viability of this vast, societal investment, in light of inherent uncertainties and their exposure to service and even extreme loadings. One of the principal means of insuring the robustness and longevity of infrastructure is to strategically deploy smart sensors in them. Therefore, the objective is to develop novel, durable, smart sensors that are especially applicable to major infrastructure and the facilities to validate their reliability and long-term functionality. In some cases, this implies the development of new sensing elements themselves, while in other cases involves innovative packaging and use of existing sensor technologies. In either case, a parallel focus will be the integration and networking of these smart sensing elements for reliable data acquisition, transmission, and fusion, within a decision-making framework targeting efficient management and maintenance of infrastructure systems. In this paper, prudent and viable sensor and health monitoring technologies have been developed and used in several large structural systems. Discussion will also include several practical bridge health monitoring applications including their design, construction, and operation of the systems.

Viability and Luciferase Activity of Freeze-Dried Recombinant Biosensor Cells for Detecting Aromatic Hydrocarbons

  • Kim, Mi-Na;Park, Hoo-Hwi;Lim, Woon-Ki;Shin, Hae-Ja
    • Biomedical Science Letters
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    • v.9 no.4
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    • pp.195-201
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    • 2003
  • Aromatic hydrocarbons are of major concern among genotoxic chemicals due to their toxicity and persistence. Some microorganisms can utilize aromatic hydrocarbons as carbon and energy sources by inducing expression of catabolic operon(s). The XylR regulatory protein activates transcription of the catabolic enzymes to degrade BTEX (benzene, toluene, ethylbenzene, and xylene) from its cognate promoters, Pu and Ps upon exposure of the cells to the aromatic hydrocarbons. The activity of XylR on the promoters was previously monitored using luciferase luc reporter system. The xylR, its promoter Pr and the promoter Po for the phenolic compound catabolic operon were introduced upstream of firefly luciferase luc in the pGL3b vector to generate about 7.1 kb of pXRBTEX. Here E. coli harboring the plasmid was freeze-dried under various conditions to fin,d optimal conditions for storage and transport. The cell viability and luciferase activity were maintained better, when the cells were freeze-dried at -7$0^{\circ}C$ in the addition of the 10% skim milk or 12% sucrose. However, coaddition of protectants such as 10% skim milk plus 10% glucose or 12% sucrose plus 10% glucose, resulted in much better viability and bioluminescence activity compared with the effect of single addition of each protectant. In addition, it was shown that the freeze-dried cells maintained almost intact bioluminescent activities and cell viability for at least 1 week after freeze-drying. This work demonstrated that the properly freeze-dried recombinant bacterial cells could be utilized as a whole-cell biosensor for simple and rapid monitoring of BTEX in the environment.

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In vitro Pollen Performance of Pinus densiflora and P. rigida: Temperature and Medium Nutrient Effects

  • Lee, Young-Keun;Kim, Yong-Ok;Booth, Thomas;Lee, Eun Ju
    • The Korean Journal of Ecology
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    • v.25 no.6
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    • pp.389-393
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    • 2002
  • Effects of environmental factors on in vitro pine pollen performance were investigated. Pinus densiflora and P. rigida pollen grains collected at Mt. Kwanak, Korea were used. Three environmental factors, such as pollen storage temperature, pollen culture temperature and nutrient condition in medium, were tested. To determine the storage temperature effects on pollen viability, pine pollen was stored at $-70^{\circ}C$, $-12^{\circ}C$, $4^{\circ}C$ and $22^{\circ}C$. Pollen viability was substantially extended at the storage temperatures of $-12^{\circ}C$ and $4^{\circ}C$ for more than 300 days. To elucidate the culture temperature effects on pine pollen germination and tube growth, pollen grains were cultured at the temperatures from $5^{\circ}C$ to $40^{\circ}C$ at $5^{\circ}C$intervals. The germination rate and tube growth were highest at $25^{\circ}C$ and decreased above $30^{\circ}C$. To investigate boron and sucrose effects on pollen tube growth, the pollen was cultured at different sucrose and boric acid concentrations. Germination rate was optimal in germination medium containing 3 or 5$\%$ sucrose with 0.01 $\%$ boric acid. These results indicate that the pine pollen can be stored for considerable length of time without noticeable loss of viability at storage temperature below or near $0^{\circ}C$. Optimal germination medium conditions were established for pine pollen. Therefore, pine pollen can be used for many biological and environmental monitoring researches.

Modified Design of Anterolateral Thigh Free Flap for Hypopharyngeal Reconstruction (하인두 재건을 위한 전외측 대퇴부 유리 피판의 변형된 도안)

  • Kim, Sung-Chan;Kim, Eun-Key
    • Archives of Reconstructive Microsurgery
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    • v.21 no.1
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    • pp.14-20
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    • 2012
  • Purpose: Defect after ablation of hypopharyngeal cancer often requires reconstruction by free tissue transfer. Since neo-hypopharynx is totally buried, various methods have been suggested for monitoring. We propose a modified design of anterolateral thigh (ALT) free flap for reconstruction of pharyngolaryngectomy defect, which has an exteriorized part for clinical monitoring and allows for primary closure. Materials and Methods: Three consecutive patients with hypopharyngeal cancer were reconstructed with ALT flap with modified design: 1) distal part of flap was elongated into fusiform shape and used as exteriorized monitoring segment with a deepithelized bridge and 2) proximal part was designed as curve so the maximum width of the flap was reduced to less than 10 cm. Results: Patient 1, 2 had uneventful postoperative course with healthy skin color and fresh pin prick bleeding. In patient 3, defect after cancer ablation was shorter than usual and deepithelized bridge was longer. When the general hemodynamic status of the patient was aggravated in postoperative course, the color of monitoring skin was changed. Viability of the whole flap was confirmed by endoscopy. However, leakage developed after 3 weeks and repair was necessary. In all patients the donor sites were closed primarily. Conclusion: By the modified design of ALT flap, clinical monitoring can be possible by examining exteriorized monitoring flap and also donor site can be closed primarily. However possibility of false positive exists and technical caution and patient selection is needed because of danger of leakage.

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Simple geometrical model to analyze the motion detection of bridges based-GPS technique: case study Yonghe Bridge

  • Kaloop, Mosbeh R.;Li, Hui
    • Structural Engineering and Mechanics
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    • v.36 no.2
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    • pp.129-147
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    • 2010
  • This study deals with the viability of using a designed geometrical model consists of plane, polar coordinates (PC) and span length in the determination of bridges deformation. The data of a Tianjin Yonghe bridge located in the southern part of China as collected by RTK-DGPS technique and Accelerometer were used in the analysis. Kalman filter and fast Fourier transformation (FFT) analyses were used to determine the frequency. The results indicate that the designed plane and PC geometrical model are easy to calculate the long-time structural deformation monitoring. In addition, the observed frequency using GPS with the rate of 20 Hz doesn't give correction natural frequency of the observation structures.

A Wind Turbine Fault Detection Approach Based on Cluster Analysis and Frequent Pattern Mining

  • Elijorde, Frank;Kim, Sungho;Lee, Jaewan
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.8 no.2
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    • pp.664-677
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    • 2014
  • Wind energy has proven its viability by the emergence of countless wind turbines around the world which greatly contribute to the increased electrical generating capacity of wind farm operators. These infrastructures are usually deployed in not easily accessible areas; therefore, maintenance routines should be based on a well-guided decision so as to minimize cost. To aid operators prior to the maintenance process, a condition monitoring system should be able to accurately reflect the actual state of the wind turbine and its major components in order to execute specific preventive measures using as little resources as possible. In this paper, we propose a fault detection approach which combines cluster analysis and frequent pattern mining to accurately reflect the deteriorating condition of a wind turbine and to indicate the components that need attention. Using SCADA data, we extracted operational status patterns and developed a rule repository for monitoring wind turbine systems. Results show that the proposed scheme is able to detect the deteriorating condition of a wind turbine as well as to explicitly identify faulty components.

Neuroprotective Effects by Nimodipine Treatment in the Experimental Global Ischemic Rat Model: Real Time Estimation of Glutamate

  • Choi, Seok-Keun;Lee, Gi-Ja;Choi, Sam-Jin;Kim, Youn-Jung;Park, Hun-Kuk;Park, Bong-Jin
    • Journal of Korean Neurosurgical Society
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    • v.49 no.1
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    • pp.1-7
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    • 2011
  • Objective: Glutamate is a key excitatory neurotransmitter in the brain, and its excessive release plays a key role in the development of neuronal injury. In order to define the effect of nimodipine on glutamate release, we monitored extracellular glutamate release in real-time in a global ischemia rat model with eleven vessel occlusion. Methods: Twelve rats were randomly divided into two groups: the ischemia group and the nimodipine treatment group. The changes of extracellular glutamate level were measured using microdialysis amperometric biosensor, in coincident with cerebral blood flow (CBF) and electroencephalogram. Nimodipine (0.025 ${\mu}g$/100 gm/min) was infused into lateral to the CBF probe, during the ischemic period. Also, we performed Nissl staining method to assess the neuroprotective effect of nimodipine. Results: During the ischemic period, the mean maximum change in glutamate concentration was $133.22{\pm}2.57\;{\mu}M$ in the ischemia group and $75.42{\pm}4.22\;{\mu}M$ (p<0.001) in the group treated with nimodipine. The total amount of glutamate released was significantly different (P<0.001) between groups during the ischemic period. The %cell viability in hippocampus was $47.50{\pm}5.64$ (p<0.005) in ischemia group, compared with sham group. But, the %cell viability in nimodipine treatment group was $95.46{\pm}6.60$ in hippocampus (p<0.005). Conclusion: From the real-time monitoring and Nissl staining results, we suggest that the nimodipine treatment is responsible for the protection of the neuronal cell death through the suppression of extracellular glutamate release in the 11-VO global ischemia model of rat.

Intraoperative Neurophysiologic Testing of the Perigastric Vagus Nerve Branches to Evaluate Viability and Signals along Nerve Pathways during Gastrectomy

  • Kong, Seong-Ho;Kim, Sung Min;Kim, Dong-Gun;Park, Kee Hong;Suh, Yun-Suhk;Kim, Tae-Han;Kim, Il Jung;Seo, Jeong-Hwa;Lim, Young Jin;Lee, Hyuk-Joon;Yang, Han-Kwang
    • Journal of Gastric Cancer
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    • v.19 no.1
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    • pp.49-61
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    • 2019
  • Purpose: The perigastric vagus nerve may play an important role in preserving function after gastrectomy, and intraoperative neurophysiologic tests might represent a feasible method of evaluating the vagus nerve. The purpose of this study is to assess the feasibility of neurophysiologic evaluations of the function and viability of perigastric vagus nerve branches during gastrectomy. Materials and Methods: Thirteen patients (1 open total gastrectomy, 1 laparoscopic total gastrectomy, and 11 laparoscopic distal gastrectomy) were prospectively enrolled. The hepatic and celiac branches of the vagus nerve were exposed, and grabbing type stimulation electrodes were applied as follows: 10-30 mA intensity, 4 trains, $1,000{\mu}s/train$, and $5{\times}$frequency. Visible myocontractile movement and electrical signals were monitored via needle probes before and after gastrectomy. Gastrointestinal symptoms were evaluated preoperatively and postoperatively at 3 weeks and 3 months, respectively. Results: Responses were observed after stimulating the celiac branch in 10, 9, 10, and 6 patients in the antrum, pylorus, duodenum, and proximal jejunum, respectively. Ten patients responded to hepatic branch stimulation at the duodenum. After vagus-preserving distal gastrectomy, 2 patients lost responses to the celiac branch at the duodenum and jejunum (1 each), and 1 patient lost response to the hepatic branch at the duodenum. Significant procedure-related complications and meaningful postoperative diarrhea were not observed. Conclusions: Intraoperative neurophysiologic testing seems to be a feasible methodology for monitoring the perigastric vagus nerves. Innervation of the duodenum via the celiac branch and postoperative preservation of the function of the vagus nerves were confirmed in most patients.

Performance indicator of the atmospheric corrosion monitor and concrete corrosion sensors in Kuwait field research station

  • Husain, A.;Al-Bahar, Suad Kh.;Salam, Safaa A. Abdul
    • Smart Structures and Systems
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    • v.17 no.6
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    • pp.981-994
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    • 2016
  • Two field research stations based upon atmospheric corrosivity monitoring combined with reinforced concrete corrosion rate sensors have been established in Kuwait. This was established for the purpose of remote monitoring of building materials performance for concrete under Kuwait atmospheric environment. The two field research sites for concrete have been based upon an outcome from a research investigation intended for monitoring the atmospheric corrosivity from weathering station distributed in eight areas, and in different regions in Kuwait. Data on corrosivity measurements are essential for the development of specification of an optimized corrosion resistance system for reinforced concrete manufactured products. This study aims to optimize, characterize, and utilize long-term concrete structural health monitoring through on line corrosion measurement and to determine the feasibility and viability of the integrated anode ladder corrosion sensors embedded in concrete. The atmospheric corrosivity categories supported with GSM remote data acquisition system from eight corrosion monitoring stations at different regions in Kuwait are being classified according to standard ISO 9223. The two nominated field sites where based upon time of wetness and bimetallic corrosion rate from atmospheric data where metals and rebar's concrete are likely to be used. Eight concrete blocks with embeddable anodic ladder corrosion sensors were placed in the atmospheric zone adjacent to the sea shore at KISR site. The anodic ladder corrosion rate sensors for concrete were installed to provide an early warning system on prediction of the corrosion propagation and on developing new insights on the long-term durability performance and repair of concrete structures to lower labor cost. The results show the atmospheric corrosivity data of the environment and the feasibility of data retrieval of the corrosion potential of concrete from the embeddable sets of anodic ladder corrosion sensors.