• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1162-1162
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• 2001

Whereas NIR spectroscopy has been applied in agriculture for more than 20 years, few studies refer to those plant substances occurring only in smaller amounts. Nevertheless there is a growing interest today to support efficiently activities in the production of high-quality medicinal and spice plants by this fast and non-invasive method. Therefore, it was the aim of this study to develop new NIR methods for the reliable prediction of secondary metabolites found as valuable substances in various plant species. First, sophisticated NIR methods were established to perform fast quality analyses of intact fennel, caraway and dill fruits deriving from single-plants [1]. Later on, a characterization of several leaf drugs and the corresponding fresh material has been successfully performed. In this context robust calibrations have been developed for dried peppermint, rosemary and sage leaves for the determination of their individual essential oil content and composition [2]. A specially adopted NIR method has been developed also for the analysis of carnosic acid in the leaves of numerous rosemary and sage gene bank accessions. Carnosic acid is an antioxidative substance for which several health promoting properties including cancer preservation are assumed. Also some other calibrations have been developed for non-volatile substances such as aspalathin (in unfermented rooibos leaves), catechins (in green tea) and echinacoside (in different Echinacea species) [3]. Some NIR analyses have also been successfully performed on fresh material, too. In spite of the fact that these measurements showed less accuracy in comparison to dried samples, the calibration equations are precise enough to register the individual plant ontogenesis and genetic background. Based on the information received, the farmers and breeders are able to determine the right harvest time (when the valuable components have reached their optimum profile) and to select high-quality genotypes during breeding experiments, respectively. First promising attempts have also been made to introduce mobile diode array spectrometers to collect the spectral data directly on the field or in the individual natural habitats. Since the development of reliable NIRS methods in this special field of application is very time-consuming and needs continuous maintenance of the calibration equations over a longer period, it is convenient to supply the corresponding calibration data to interested user via NIRS network. The present status of all activities, preformed in this context during the last three years, will be presented in detail.

• 대한의용생체공학회:의공학회지
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• 제24권1호
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• pp.37-44
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• 2003

본 실험연구에서는 정상유동상태에서 새롭게 설계된 자가팽창성 그래프트 스텐트의 수력학적 성능을 평가하고자 하였다. 코팅 재질이 다른 두 개의 그래프트 스텐트와 한 개의 타이티놀 금속스텐트가 실험에 사용되었으며, 유량이 가자 5, 10, 15 1/min에서 스텐트 전후에서의 압력변화 및 속도분포를 측정하였다. 스텐트 삽입에 의한 압력손실은 유량이 증가함에 지수적으로 증가하였다. 특히 15 1/min의 유량에서 다공성 PTFE 그래프트 스텐트와 TiNi 금속스텐트의 압력손실은 거의 동일하나 PU 그래프트 스텐트는 약 6배 이상의 현저한 증가를 보이고 있다. 스텐트 후류에서의 속도분포는 다공성 PTFE 그래프트 스텐트와 TiNi 금속스텐트는 유량에 관계없이 유사한 형태를 보여주고 있다. 그러나, PU 그래프트 스텐트에서는 특히 유량이 10 1/min 이상에서 속도분포가 비대칭적이고 관 중심에서의 상대적인 낮은 유속을 보여주고 있으며, 결과적으로 벽면전단응력 및 수직응력의 증가론 초래하고 있다. 이와같이 PU 그래프트 스텐트의 상대적으로 낮은 수력학적 성능은 스텐트가 보다 작은 관에 삽입되었을때 코팅재질의 낮은 유연성으로 인하여 스텐트의 표면에 주름이 발생하여 유동단면이 비대칭적으로 되고 벽면의 조도가 증가하며, 관벽과 스텐트와 틈새가 존재하여 제트류가 형성되기 때문으로 해석된다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권11호
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• pp.1547-1554
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• 2009

This study was carried out to evaluate the effects of dietary supplementation of different sources of chromium on growth performance, blood profile and carcass trait in growing-finishing pigs. A total of 200 growing pigs (Landrace${\times}$Yorkshire)${\times}$Duroc, average initial weight 8.5 kg) were allotted to 5 treatments with 4 replicates per treatment and 10 pigs per replicate. Five treatments were designated as follows according to the source of chromium. i) Control (No chromium): corn-soybean meal based basal diet, ii) $CrCl_{3}$: control diet+200 ppb Cr as $CrCl_{3}$, iii) CrPic: control diet+200 ppb Cr as Cr picolinate, iv) CrMet-1: control diet+100 ppb Cr as Cr methionine, and v) CrMet-2: control diet+200 ppb Cr as Cr methionine. After the feeding trial, three pigs per replicate (12 pigs per treatment) were slaughtered for the evaluation of carcass traits. Average daily gain (ADG), average daily feed intake (ADFI), and feed: gain ratio (F/G) were not different (p>0.05) among dietary Cr sources. However, whole-period ADG of pigs fed CrPic, CrMet-1 and CrMet-2 diets was higher (p<0.05) than for the control diet. Nutrient digestibility was not different (p>0.05) among dietary Cr sources, but the nutrient digestibility of pigs fed CrPic, CrMet-1 and CrMet-2 diets was higher (p<0.05) than for the control diet. BUN level decreased with more magnitude (p<0.05) in pigs fed Cr during the 20 to 50 kg period. Although both serum cholesterol and triglyceride were different (p<0.05) among treatments, there was no consistent response that could be related to the dietary Cr sources regardless of growth phase. However, the overall data suggested that serum cholesterol level increased as BW of pigs increased. Blood total protein (TP) increased (p<0.05) in pigs fed Cr only during the 90-110 kg phase, and blood creatinine (Creat) level was higher in $CrCl_{3}$ and CrPic treatments than in the control only during the 90-110 kg phase. Backfat thickness was thinner (p<0.05) in pigs fed CrMet-2 than in the control treatment. Therefore, lean percentage was higher (p<0.05) in CrMet-2 than in control pigs. However, dressing percentage and Longissimus muscle area (LMA) were not different (p>0.05) among treatments. In conclusion, dietary supplementation of 200 ppb Cr, via either CrPic or CrMet, improved pig growth performance and nutrient digestibility. Moreover, dietary CrMet supplementation for the growing-finishing pig is evidently remarkable for improving both lean percentage of the carcass and backfat thickness.

• Asian Pacific Journal of Cancer Prevention
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• 제15권1호
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• pp.495-499
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• 2014

Estrogens are considered the major breast cancer risk factor, and the carcinogenic potential of estrogens might be attributed to DNA modification caused by derivatives formed during metabolism. $17{\beta}$-estradiol ($E_2$), the main steroidal estrogen present in women, is metabolized via two major pathways: formation of 2-hydroxyestradiol (2-OH $E_2$) and 4-hydroxyestradiol ($4-OH\;E_2$) through the action of cytochrome P450 (CYP) 1A1 and 1B1, respectively. Previous reports suggested that $2-OH\;E_2$ has putative protective effects, while $4-OH\;E_2$ is genotoxic and has potent carcinogenic activity. Thus, the ratio of $2-OH\;E_2/4-OH\;E_2$ is a critical determinant of the toxicity of $E_2$ in mammary cells. In the present study, we investigated the effects of berberine on the expression profile of the estrogen metabolizing enzymes CYP1A1 and CYP1B1 in breast cancer MCF-7 cells. Berberine treatment produced significant induction of both forms at the level of mRNA expression, but with increased doses produced 16~ to 52~fold greater induction of CYP1A1 mRNA over CYP1B1 mRNA. Furthermore, berberine dramatically increased CYP1A1 protein levels but did not influence CYP1B1 protein levels in MCF-7 cells. In conclusion, we present the first report to show that berberine may provide protection against breast cancer by altering the ratio of CYP1A1/CYP1B1, could redirect $E_2$ metabolism in a more protective pathway in breast cancer MCF-7 cells.

• 대한약침학회지
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• 제19권1호
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• pp.37-44
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• 2016

Objectives: This study examined the relative efficacies of a derivative of betulinic acid (dBA) and its poly (lactide-co-glycolide) (PLGA) nano-encapsulated form in A549 lung cancer cells in vivo and in co-mutagen [sodium arsenite (SA) + benzo[a]pyrene (BaP)]-induced lung cancer in mice in vivo. Methods: dBA was loaded with PLGA nanoparticles by using the standard solvent displacement method. The sizes and morphologies of nano-dBA (NdBA) were determined by using transmission electron microscopy (TEM), and their intracellular localization was verified by using confocal microscopy. The binding and interaction of NdBA with calf thymus deoxyribonucleic acid (CT-DNA) as a target were analyzed by using conventional circular dichroism (CD) and melting temperature (Tm) profile data. Apoptotic signalling cascades in vitro and in vivo were studied by using an enzyme-linked immunosorbent assay (ELISA); the ability of NdBA to cross the blood-brain barrier (BBB) was also examined. The stage of cell cycle arrest was confirmed by using a fluorescence-activated cell-sorting (FACS) data analysis. Results: The average size of the nanoparticles was ~ 110 nm. Confocal microscopy images confirmed the presence of NdBA in the cellular cytoplasm. The bio-physical properties of dBA and NdBA ascertained from the CD and the Tm profiles revealed that NdBA had greater interaction with the target DNA than dBA did. Both dBA and NdBA arrested cell proliferation at G0/G1, NdBA showing the greater effect. NdBA also induced a greater degree of cytotoxicity in A549 cells, but it had an insignificant cytotoxic effect in normal L6 cells. The results of flow cytometric, cytogenetial and histopathological studies in mice revealed that NdBA caused less nuclear condensation and DNA damage than dBA did. TEM images showed the presence of NdBA in brain samples of NdBA fed mice, indicating its ability to cross the BBB. Conclusion: Thus, compared to dBA, NdBA appears to have greater chemoprotective potential against lung cancer.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제14권9호
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• pp.916-920
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• 2008

개인 컴퓨팅 환경은 사용자에 특화된 응용프로그램 및 설정, 사용자 데이타 등을 총칭하는 개념으로써, 노트북, UMPC 등의 휴대용 컴퓨팅 H/W는, 어떤 곳에서도 항상 개인에게 특화된 컴퓨팅 환경에서 작업 가능하도록 소형화, 경량화가 진행되고 있다. 그러나, 최근 가상화 기술 및 휴대용 스토리지 기술을 이용한 다양한 개인 컴퓨팅 환경 이동성 지원 기술이(c.f. VMWare Pocket ACE[1], Mojopac[2], u-PC[3] 등) 등장함으로써, 공용 PC 상에 자신의 컴퓨팅 환경을 로딩하여 사용할 수 있도록 지원하는 기술이 주목받고 있다. 특히, 본 논문의 이전 연구로써, u-PC[3]에서는 UPnP 및 iSCSI 프로토콜을 이용하여 사용자 영역 네트워크(PAN)상에서 자동 검색 및 구성이 가능한 무선 스토리지 기술과, Windows 운영체제 상에서 구동 가능한 개인 컴퓨팅 환경 이동성지원 기술을 제안하였다. 그러나, 기존의 u-PC 기술에서는, Windows 운영체제 상 Filter Driver 계층에서 IRP(I/O Request Packet) forwarding을 이용하여 개인 컴퓨팅 환경의 이동성 지원 기술을 구현함으로써, 제한적인 응용 프로그램만 구동 가능한 한계점이 있었다. 본 논문에서는 Windows 운영체제 상 System Call Hooking 기법을 이용하여 운영체제 가상화 기술을 구현하고, 개인 컴퓨팅 환경의 이동성을 지원함으로써, 기존 논문에서의 한계성을 극복하였으며, VMWare Pocket ACE, Mojopac 등의 단점인 개인 컴퓨팅 환경의 구동 초기화 오버 헤드를 극복하였다. 또한, Ultra Wide Band (UWB) 기반 고속 무선 네트워크 상 적용 가능한 무선스토리지 기술을 개발하고, 개선된 u-PC 플랫폼을 연동하여 다양한 형태의 개인 컴퓨팅 환경 사용 모델을 제시함으로써 기술의 적용성을 검증하였다.

• 한국어병학회지
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• 제27권1호
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• pp.1-9
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• 2014

저수온기만 넙치에 대량 폐사를 일으키는 바이러스성 출혈성 패혈증을 폐사가 발생하는 $15^{\circ}C$, 폐사가 발생하지 않는 $20^{\circ}C$에서 인공감염시켜 넙치의 면역 유전자 발현 profile을 cDNA microarray 분석하였으며, 특히 저수온기에 폐사가 나타나는 원인을 면역 유전자 발현과 관련시켜 알아보고자 하였다. $15^{\circ}C$, $20^{\circ}C$의 감염 세포구에 공통으로 발현되는 유전자는 MHC class I, IL-8, myeloperoxidase 및 endonuclease G-like 유전자로 모든 세포표면에 존재하여 항원을 제시하거나 호중구 주화성을 자극하는 유전자들이었다. 항원 가공 및 제시, 항체 생성에 관여하는 MHC class II, immunoglobulin (Ig)과 retinoblastoma 등의 유전자는 $20^{\circ}C$에서는 발현이 증가하였으나 $15^{\circ}C$에서는 발현이 감소되었다. 이로부터 폐사가 발생하지 않는 $20^{\circ}C$는 바이러스 감염초기의 항원 제시, MHC class I과 II에 의한 항원제시, apoptosis 및 이후의 항체 생산이 정상적으로 이루어져 폐사가 발생하지 않는 것으로 생각되었다. 그러나 폐사가 발생하는 $15^{\circ}C$에서는 MHC class I매개의 항원 제시와 탐식 작용등의 선천 면역은 이루어지나 macrophage에 의한 MHC class II매개의 항원 제시와 apoptosis저하, 항체 생산 관련 유전자의 발현저하가 관찰되어 초기 macrophage에 의한 항원제시의 실패로 적응 면역이 제대로 활성화되지 않아 폐사가 발생한 것으로 사료된다.

• 한국지반환경공학회 논문집
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• 제19권5호
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• pp.23-31
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• 2018

최근 해양구조물의 건설과 간척지의 개발이 활발히 이루어짐에 따라 해안 구조물 시공 및 간척지 개발 시 필요한 준설작업은 중요 사업으로 대두되고 있다. 이에 보다 정확하고 정량적인 준설물량의 예측과 산출이 더욱 중요해지고 있다. 해저 지층정보는 시추조사를 통하여 지점별 1차원 지층 정보를 얻는 것이 일반적이며, 준설물량은 시추조사를 통하여 얻어진 종결심도나 두께를 토대로 3차원 지반 정보화 프로그램을 활용하여 산출하게 된다. 이 과정에서 대상 지역 특정 지층의 2차원적 종결심도를 결정하기 위해 1차원적인 시추공 데이터들을 공간보간한 입력DB를 구축해야 한다. 입력DB는 시추공 활용 여부와 공간보간하는 대상에 따라 구축방안이 상이하다. 따라서 본 연구는 대표적인 간척지 지역인 새만금지역과 군산외항 앞바다의 시추공 데이터를 활용하여 입력 DB 구축 방안에 따른 지층의 3차원 가시화 및 정량적인 준설물량을 비교분석하였다. 그 결과 지층의 두께를 공간보간한 구축 방안이 두 지역 모두 정밀도가 높았고 3차원 가시화 결과도 잘 나타났다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권15호
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• pp.6227-6232
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• 2014

Background: Oral squamous cell carcinoma (OSCC) remains as one of the most difficult malignancies to control because of its high propensity for local invasion and cervical lymph node dissemination. The aim of present study was to evaluate the efficacy of novel pH and temperature sensitive doxorubicin-methotrexate-loaded nanoparticles (DOX-MTX NP) in terms of their potential to change the VEGF-C expression profile in a rat OSCC model. Materials and Methods: 120 male rats were divided into 8 groups of 15 animals administrated with 4-nitroquinoline-1-oxide to induce OSCCs. Newly formulated doxorubicin-methotrexate-loaded nanoparticles (DOX-MTX NP) and free doxorubicin were IV and orally administered. Results: Results indicated that both oral and IV forms of DOX-MTX-nanoparticle complexes caused significant decrease in the mRNA level of VEGF-C compared to untreated cancerous rats (p<0.05). Surprisingly, the VEGF-C mRNA was not affected by free DOX in both IV and oral modalities (p>0.05). Furthermore, in DOX-MTX NP treated group, less tumors characterized with advanced stage and VEGF-C mRNA level paralleled with improved clinical outcome (p<0.05). In addition, compared to untreated healthy rats, the VEGF-C expression was not affected in healthy groups that were treated with IV and oral dosages of nanodrug (p>0.05). Conclusions: VEGF-C is one of the main prognosticators for lymph node metastasis in OSCC. Down-regulation of this lymph-angiogenesis promoting factor is a new feature acquired in group treated with dual action DOX-MTX-NPs. Beside the synergic apoptotic properties of concomitant use of DOX and MTX on OSCC, DOX-MTX NPs possessed anti-angiogenesis properties which was related to the improved clinical outcome in treated rats. Taking together, we conclude that our multifunctional doxorubicin-methotrexate complex exerts specific potent apoptotic and anti-angiogenesis properties that could ameliorate the clinical outcome presumably via down-regulating dissemination factor-VEGF-C expression in a rat OSCC model.

• Asia pacific journal of information systems
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• 제22권4호
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• pp.125-149
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• 2012

As the smart phone is propagating rapidly, the importance of mobile advertisement has also grown. One of the main characteristics of the Internet and smart phone advertising is that they can deliver personalized advertisements to each customer. The smart phone enables the identification of additional personalized information such as the customer's location and the accessibility to the site at any place any time. As the Internet platform becomes richer, firms that offer the ad services via the wired PC Internet and wireless smart phone are seeking various types of personalized ads. However, their service platform and Information and Communication Technology (ICT) platform should be suitable to the characteristics of personalized ads. This research explores various types of personalized ad methods and evaluates their adequacy encompassing four types of ad service platforms (such as search portal, news portal, e-mall servers, and SNS) and two types of ICT platforms (PC Internet and smart phone). To this end, we classified the personalized ads into seven types: three basic types and four composite types. The basic types of ad methods are identified by considering the current activity that the customer is engaged, the individual profile and log history, and the customer's current location or planning location. Four composite types of ad methods are constructed as the combination of these basic types. For those types of ad methods, we evaluate whether each ad method adequately maps with four types of ad service platforms and two types of ICT platforms. We proposed a metric of evaluation and demonstrated the concept with illustrative numbers. Specifically, we analyze and compare personalized ad methods in three ways. Firstly, the possibility of implementing a personalized ad method on the platform is analyzed to confirm the degree of suitability. Secondly, the value of personalized ad method is analyzed based on the customer accessibility. Lastly, expected effectiveness for each personalized ad method is computed by multiplying the possibility and the value. Through this kind of analysis, the ad service providers as well as advertising companies can evaluate what kinds of personalized ad methods and platforms are possible and suitable to maximize their ad effectiveness on the Internet and smart phone platforms.