• Biomedical Science Letters
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• v.20 no.4
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• pp.209-220
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• 2014

Vascular endothelial growth factor (VEGF) is a key factor involved in the induction of angiogenesis and has become an attractive target for anti-angiogenesis therapies. The purpose of this study was to elucidate the anti-angiogenic activity of Rubus coreanus Miquel water extract (RCME). Rubus coreanus Miquel has long been employed as a traditional medicine, and recent studies have demonstrated that it has measureable biological activities. Thus, we investigated for the first time the effect of RCME on angiogenesis and its underlying signaling pathways. The effects of RCME were tested on in vitro models of angiogenesis, namely, proliferation, migration, invasion and tube formation of human umbilical vein endothelial cells as well as an ex vivo model of vessel sprouting from the rat aorta in response to VEGF. We observed that VEGF-induced angiogenesis was strongly suppressed by RCME treatment compared to that of the control group. Moreover, we found that RCME inhibited VEGF-induced activation of matrix metalloproteinases and phosphorylation of extracellular signal-regulated kinase and p38, and also effectively inhibited phosphorylation of VEGF receptor 2. These results indicated that RCME inhibits angiogenesis by suppressing phosphorylation of the VEGF receptor and may be useful for the treatment of angiogenesis-dependent diseases such as cancer and diabetic retinopathy.

• Archives of Plastic Surgery
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• v.33 no.4
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• pp.510-513
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• 2006

Purpose: The Rectus abdominis muscle free flap is utilized in various reconstruction surgeries due to easiness in harvesting, consistency of vascular pedicle and reduced donor site morbidity. But rarely, femoral nerve injury during rectus abdominis harvesting can be resulted. We report a case of femoral nerve injury after rectus muscle harvesting and discuss the injury mechanism with the follow-up process of this injury. Methods: To reconstruct the defect of middle cranial base after wide excision of cystic adenocarcinoma of the external ear, rectus muscle free flap was havested in usual manner. To achieve a long vessel, inferior epigastric artery was dissected to the dividing portion of femoral artery and cut. Results: One week after the surgery, the patient noted sensory decrease in the lower leg, weakness in muscle strength, and disabilities in extension of the knee joint resulting in immobilization. EMG and NCV results showed no response on stimulation of the femoral nerve of the left leg, due to the defects in femoral nerve superior to the inguinal ligament. With routine neurologic evaluations and physical therapy, on the 75th day after the operation, the patient showed improvement in pain, sensation and muscle strength, and was able to move with walking frame. In 6 months after the operation, recovery of the muscle strength of the knee joint was observed with normal flexion and extension movements. Conclusion: Rarely, during dissection of the inferior epigastric artery, injuries to the femoral nerve can be resulted, probably due to excessive traction or pressure from the blade of the traction device. Therefore, femoral nerve injury can be prevented by avoiding excessive traction during surgery.

• Fire Science and Engineering
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• v.20 no.3 s.63
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• pp.29-34
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• 2006

The flash and fire point are the most important combustible properties used to determine the potential for the fire and explosion hazards of flammable material. The flash point is defined as the lowest temperature at which a flammable liquid gives off sufficient vapor to form an ignitable mixture with air near its surface or within a vessel. The fire point is the temperature of the flammable liquid at which there will be flaming combustion, sustained 5 seconds in response to the pilot flame. In this study, the flash points and fire points were measured to present raw data of the flammable risk assessment for acids, using Pensky-Martens Closed Cup(C.C.) apparatus (ASTM-D93) and Tag Open-cup (O.C.) apparatus(ASTM D 1310-86). The measured fire points were compared with the estimated values based on 1.11 times stoichiometric concentration. The values calculated by the proposed equation were in good agreement with measured values.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2002.11a
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• pp.95-100
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• 2002

The wing in ground effect (WIG) ship is an energy saying vessel that uses the lift from its air-wing along with the lift increase from the ground effect by flying low above the sea surface. The WIG Ship should consist of thin plate in order to float on the sea and to fly in the air. Therefore, the structure of WIG, Ship has very thin and light shell plate and stiffener like stringer and frame has comparatively large cross section area. This structure makes shell plate nearly pure shear field when shell plate is pressed by in-plane load. This complex thin plate structure of WIG Ship can he considered as a closed section beam which makes it possible to analyze structure response of WIG Ship affected by shear load and bending load. In this respect, the present study will show basic theory for analysing shear stress and focus on the analysis of structure strength of model WIC Ship's wing.

• Journal of Microbiology and Biotechnology
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• v.14 no.4
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• pp.707-714
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• 2004

How to improve the cell culture method on scaffolds is important in the tissue engineering fileld. In this study, we optimized seeding and culture methods to vascular smooth muscle cells (VSMCs) on biodegradable polymer scaffold. The primary culture of VSMCs obtained from canine external jugular vein was accomplished by applying the explant-derived method. The primary cultured VSMCs were seeded into scaffolds and then cultured by using various different methods; static or dynamic seeding, static or dynamic culture. The difference in proliferative response of VSMCs was analyzed with an alamar blue assay. Cell-polymer construct was examined by histochemical method and scanning electron microscopy. Mesh type scaffold ($10 \times 10 \times0.4 mm$) was made of polyglycolic acid (PGA) suture thread. The PGA mesh type scaffold was 45% in porosity, and 0.03 g in weight. The primary cultured VSMCs were confirmed with immunohistochemical staining using monoclonal anti-$\alpha$-smooth muscle actin. The density and distribution of proliferated VSMCs within the scaffold and cellular adherence on the surface of the scaffold showed better results in the static seeding condition than in the dynamic condition. Under the same condition of seeding method as the static condition, the dynamic culture condition showed enhanced proliferation rates of the VSMCs when compared to the static culture condition. In conclusion, to improve the VSMCs proliferation in vitro, static seeding is better than the dynamic condition. In the culture condition, however, culture under the dynamic status is better than the static condition. This was a pilot study to manufacture artificial vascular vessel by tissue engineering.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.1
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• pp.69-74
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• 2004

We have examined the relaxative response to the water extract of Rheum undulatum L. (ERU) and water extract of alcohol steamed Rheum undulatum L. (SRU) with isolated thoracic aorta from sprague dawley (SD) rat. Rat thoracic aorta was investigated in vessel segments suspended for isometric tension recording by polygraph. Responses to ERU and SRU were investigated in vessels precontracted with 5-hydroxytryptamine(5-HT). We found that the thoracic aorta segments responded to ERU and SRU with a dose-dependent vasorelaxation : The thoracic aorta segments responded to ERU and SRU with a dose-dependent vasodilation. The amounts of emodin were 0.063%, 0.076% and 0.145% in ERU and SRU, respectable. The 5-HT induced contraction at 10-4M were inhibited by 85.2±4.76% and 84.0±2.91% after addition of the 0.1mg/mL water extract of ERU and SRU. The 5-HT induced contraction at 10/sup -3/M were inhibited by 100% after 10/sup -3/M emodin. In conclusion, vasodilation effect of the water extract of Rheum undulatum L. in rat thoracic aorta was not decreased according to the processing of alcohol steamed Rheum undulatum L.

• KSBB Journal
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• v.29 no.1
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• pp.36-41
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• 2014

Skin disease is one of the most common diseases and its incidence is increasing dramatically in modern society. Specially, many attempts have been made to treat chronic skin inflammation diseases, such as psoriasis and atopic dermatitis, but effective therapies for the immune cell-mediated skin diseases, including psoriasis and atopic dermatitis have not been developed. Until recently, several drug candidates which were claimed to be effective for skin diseases have been reported, but most of them are not used to treat chronic skin disease. Especially, Psoriasis is characterized by excessive growth and aberrant differentiation of keratinocytes, but is fully reversible with appropriate therapy. The trigger of the keratinocyte response is thought to be activation of the cellular immune system, with T cells and various immune-related cytokines. Formation of new blood vessels starts with early psoriatic changes and disappears with disease clearance. Several angiogenic mediators are up-regulated in psoriasis development. Contact- and mediator-dependent factors derived from keratinocytes, mast cells and immune cells may contribute to the strong blood vessel formation of psoriasis. New technologies and experimental models provide new insights into the role of angiogenesis in psoriasis pathogenesis. TMP and its derivatives themselves effectively inhibited in vitro cell migration, tube formation, and the expression of angiogenic factors. However, TMP and its derivatives induced side effects including hemolysis and local side effects. Therefore, in an attempt to reduce the toxicity and the undesirable side effects of TMP and derivatives, a liposomal formulation was prepared and tested for its effectiveness. TMP and derivatives liposomes retained the effectiveness of TMP in vitro while side effects were reduced. These results support the conclusion that TMP effectively inhibits in vitro angiogenesis, with the possibility that use as a psoriasis relief agent.

• International Journal of Naval Architecture and Ocean Engineering
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• v.3 no.1
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• pp.27-36
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• 2011

In the proposed paper numerical calculations are carried out using two versions of a three-dimensional, timedomain panel method developed by the group of Prof. P. Sclavounos at MIT, i.e. the linear code SWAN2, enabling optionally the use of the instantaneous non-linear Froude-Krylov and hydrostatic forces and the fully non-linear SWAN4. The analytical results are compared with experimental results for three hull forms with increasing geometrical complexity, the Series 60, a reefer vessel with stern bulb and a modern fast ROPAX hull form with hollow bottom in the stern region. The details of the geometrical modeling of the hull forms are discussed. In addition, since SWAN4 does not support transom sterns, only the two versions of SWAN2 were evaluated over experimental results for the parent hull form of the NTUA double-chine, wide-transom, high-speed monohull series. The effect of speed on the numerical predictions was investigated. It is concluded that both versions of SWAN2 the linear and the one with the non-linear Froude-Krylov and hydrostatic forces provide a more robust tool for prediction of the dynamic response of the vessels than the non-linear SWAN4 code. In general, their results are close to what was expected on the basis of experience. Furthermore, the use of the option of non-linear Froude-Krylov and hydrostatic forces is beneficial for the accuracy of the predictions. The content of the paper is based on the Diploma thesis of the second author, supervised by the first one and further refined by the third one.

• Journal of the Society of Naval Architects of Korea
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• v.51 no.5
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• pp.369-379
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• 2014

Planing hull forms have significant influences on those hydrodynamic performances in calm water and in waves. Therefore, the hydrodynamic performance of a planing vessel should be predicted by model tests or theoretical calculations, and be confirmed whether it shows the performance requirements at the design stage. In this study, four planing hull forms are designed with the goal of the improvement of resistance and seakeeping performance, and 1/6.5 scale model tests are carried out in Seoul National University towing tank. The effects of design parameters such as length-to-beam ratio, deadrise angle and forebody shape on the hydrodynamic performance are investigated, based on model test results. Running attitude and resistance of model ships in calm water are also estimated by empirical formulae proposed by Savitsky (1964; 2007; 2012), and compared with the model test results. It is shown that calm water performance of non-prismatic planing hulls can be predicted well by Savitsky (2012)'s formula which improves the original Savitsky(1964/2007)'s formula by taking into account the variations of deadrise angles, and the actual angles between the hull bottom and the free surface.

• Journal of the Korean Institute of Navigation
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• v.10 no.2
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• pp.97-114
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• 1986

Ship's maneuverability is very important factor in safe ship handling and economical ship operation. Steering characteristics are consisted of course stability and maneuverability. Today in many advanced ship-building countries, they study ship's course stability, using model ship tests, such as straight line tests, rotating arm tests and Planar Motion Mechanism (PMM) etc., in tow in tanks. It is the purpose of this paper to provide ship's handlers with better understanding of steering characteristics and to help them in safe controlling and manevering . In this paper, the author simulated response of various vessels, running straight course with constant speed, and they are disturbed by small external disturbance of one degree yaw angle with no angular velocity . The author used the hydrodynamic derivtives resulted at tests of Davidson's laboratory in Stevens Institute of Technology, New Jersey, U.S.A. Course stability was evaluated and analyzed in various respects, such as block coefficient, ratio of ship's length to beam, draft and rudder area ratio etc. The obtained results are as follows : (1) The ship's course stability is affected by magnitude of block coefficient greatly. In case that the block coefficient is more than 0.7, the deviation varies at nearly same rate but the requistite time to reach the steady course is different. (2) The ship's course stability is affected by magnitude of L/B. When the dimensionless time reaches about 3, the deviation and requisite time to reach the steady course are influenced nearly same. After the dimensionless time is about 3, they change on invariable ratio. (3) The effect to course stability by L/T and RA' can be neglected. (4) The reason why thy VLCC and container feeder vessel are unstable on their course is that their block coefficient is generally more than 0.8 and the ratio of ship's length to beam is about 6.0.