• Animal cells and systems
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• 제10권3호
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• pp.137-143
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• 2006

A series of no-choice olfactory response tests using water pre-conditioned with females, with intact and surgically removed ventral glands, at various reproductive states were conducted to determine whether male Hynobius leechii discriminates among females in different reproductive states based on chemical cues. Similarly, ventral gland extracts were tested, and ventral glands were examined histologically. Males’ responses to putative odors of females in four (ovulating, ovulated, ovipositing, and oviposited) reproductive states were independently measured by: i) the latency time to initiate male behavioral response, ii) the arrival time at a fixed point of putative odor source, and iii) the staying time close to the odor point source. Male salamanders showed significant olfactory responses to recently ovulated and ovipositing female odors by quickly arriving at odor sources and staying longer at the origin of the source, but the olfactory responses to the earlier staged ovulating females and the later stage of already oviposited females were not different from controls. Olfactory responses of test males to water preconditioned by intact females or females with ventral glands excised were not different. In addition, ventral gland extracts did not induce significant olfactory responses of test males although the lumens of alveoli in ventral glands of oviposited females were smaller than those of ovulated females. These results indicate that male H. leechii recognizes ovulated and ovipositing females based on chemical cues released but not from the ventral glands.

• Applied Microscopy
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• 제27권1호
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• pp.1-12
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• 1997

Dorsal and ventral epithelium of leech, Whitemenia edentula, were observed, using both light and electron-microscope. Results are as follows. Epithelia are composed of simple columnar or irregularly-shaped epithelium, the transveral folds were seen in the cross-sectioned dorsal and ventral epithelia, but only longitudinal folds(depth $500{\mu}m$, width $350{\mu}m$) were discovered in the ventral epithelium. As to muscular tissue under the dorsal epithelium of Whitemenia edentula, that of longitudinal muscle layer is thicker and more developed than that of circular muscle layer. Seven gland cells (type-A, B, C, D, E, F and H cells) and one pigment cell (type-G cell) were discovered in the whitemenia edentula. Those were identified as three kinds of the neutral mucopolysaccharide granoles (type-A, B and F cells), two kinds of acidic mucopolysaccharide granules (type-C and D cells), and two kinds of non-reactive granules respectively (type-E and F cells). The glanules of the type-A and type-B gland cells ae electronly high-dense, and surrounded by the muscular tissue. As to the distribution of gland cells, the type-A, type-B, type-C, type-D, type-I and type-H gland cells were discovered only between the connective tissue and the circular muscle layer, while type-F gland cells were discovered only between tile circular muscle layer and the longitudinal muscle layer.

• 한국어류학회지
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• 제19권3호
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• pp.201-209
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• 2007

광학현미경상에서 복섬, Takifugu niphobles의 피부 표면은 불규칙한 주름을 가지는데, 이들 주름은 등 쪽보다 배 쪽에서 훨씬 뚜렷했다. 피부계는 외부로부터 상피층과 진피층으로 구분되며, 상피층은 다층상피층으로 상피세포, 점액세포, 곤봉상세포, 과립세포 그리고 multivacuolar gland들로 구성되어 있었다. 상피세포는 표면세포, 중간세포, 기저세포로 구분되며, 표면상피의 자유면은 미세융기로 덮여 있었다. 상피층의 선세포는 단세포선과 다세포선으로 구분되었다. 점액분비선은 다세포선의 형태로 점액세포들은 중성 당단백질의 점액물질을 함유하고 있었다. Multivacuolar gland들은 장경 약 $20{\mu}m$의 vacuole cell들로 구성되어 있었다. Vacuole cell들은 커다란 중심공포를 가지며, 잘 발달된 desmosome으로 연결되어 있었다. 점액분비선과 multivacuolar gland는 등 쪽에 비해 배 쪽에서 훨씬 발달되어 있었다. 진피층의 두께는 배 쪽 피부계의 경우에는 상피세포층의 3~5배였다. 진피층은 치밀결합조직으로 교원섬유, 섬유세포, 가시의 기저부, 색소세포 및 다수의 신경세포 분포가 확인되었다.

• Animal Systematics, Evolution and Diversity
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• 제22권1호
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• pp.17-22
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• 2006

Cryptocercus nymphs periodically groom ventral surface of their parents. The grooming might be licking-behavior to obtain secretions from the ventral surface of their parents, and some essential nutrients or hormones that facilitate nymphal development might be included in the secretions. We tried to find morphological structures for secretion outlets on the ventral surface. The deep depressions around setae were present, and their shape was an external morphological structure that liquid secretions from internal glands are likely to be well seized. There were also small holes on the depressions that might be external openings for secretions from the sternal glands. Another possible region on body surface for outlets of secretions might be the apophyses. In Cryptocercus individuals, mucous liquid on body surface was relatively highly present around coxa. The intercoxal apodemes, to which muscles are attached and which open externally between the mid and hindcoxae, might have evolved a secondary function of producing nourishment for the young.

• Applied Microscopy
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• 제39권1호
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• pp.17-25
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• 2009

본 연구에서는 에스트로겐 수용체 알파 효능제인 propyl pyrazole triol (PPT)을 수컷 생쥐에 투여하였을 때 부속 생식샘의 조직학적 변화가 유발되는지를 조사하였다. 광학현미경으로 조사하기 위해 수컷 생식기관을 고정, 탈수, 포매, 절편 과정을 거쳐 프레파라트를 완성하였다. PPT에 의해 복부쪽 전립샘, 정낭, 포피샘의 각 무게는 실험기간에 따라 감소되었다. 투여군의 경우, 복부쪽 전립샘의 샘 조직 내강은 위축되었다. 전립샘 상피조직의 형태가 단층 원주상피에서 중층 입방 상피 혹은 편평상피로 변화되었다. 투여군 전립샘과 정낭의 상피조직 아래에 있는 결합조직은 증가되었다. 특히, 투여군 정낭의 내강이 대조군에 비해 위축되었다. 포피샘의 투여군 8주에서 상피세포 높이가 감소되었다. 수컷 내에서 에스트로겐 수용체 효능제의 이런 영향을 파악함으로서 생식기관 내 에스트로겐의 생리학적 기능을 이해하는데에 도움을 줄 것으로 사료된다.

• Applied Microscopy
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• 제41권4호
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• pp.265-276
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• 2011

에스트로겐 수용체 알파 촉진제인 propyl pyra zole triol (PPT)를 투여하여 복부 전립샘, 정낭, 포피샘과 같은 수컷 생쥐 부속생식샘의 무게 및 조직학적 구조에 미치는 영향을 조사하였다. 본 연구에서는 성체 수컷 생쥐를 대상으로 casor oil에 각각 0.01 mg, 0.1 mg, 1mg의 농도로 희석한 PPT를 주 1회씩 3, 5, 8주 동안 피하주사 한 후 부속생식샘의 무게 및 조직학적 변화를 관찰하였다. 전체적으로 부속생식샘의 무게가 PPT 0.01 mg과 0.1 mg 처리군에서 증가하였지만, PPT 1.0 mg 처리군에서는 감소하였다. 전립샘의 상피조직은 대조군의 원주상피에서 처리군의 평편상피 또는 입방상피 형태로 변화하였다. PPT 처리군의 3주째부터 전립샘의 상피세포의 높이가 감소하였다. 정낭의 내강면적은 처리군에서 줄어든 형태로 나타났다. PPT 처리군에서 정낭의 상피세포의 높이는 감소되었다. PPT 1.0 mg 처리군에서는 포피샘의 샘포조직이 급격하게 위축되었다. 이러한 결과는 수컷 생쥐의 에스트로겐에 대한 생리적 기능을 이해하기 위한 실험에서 PPT 농도를 결정하는 데에 참고 자료로 활용될 수 있을 것으로 생각된다.

• 대한수의학회지
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• 제42권3호
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• pp.389-392
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• 2002

A 17-year-old female tiger (Panthera tigris altica) was found dead after suffering from continuously growing mass at the right mammary gland area. At necropsy, a firm tan mass approximately 25 cm in diameter was noted at the ventral abdomen. The mass was not fixed to the adjacent tissue and mottled tan to red on cut sections. Chains of similar nodules ranging from 2 to 5 cm in diameter were also present along the right mammary glands region. Histologically, the neoplastic masses consisted of lobules that were filled with pleomorphic neoplastic cells and separated by fibrious conntective tissue. The neoplastic cells have hyperchromatic nuclei with prominent nucleolus and moderate amount of cytoplasm. The degree of mitosis was high. Multiple areas of necrosis, hemorrhage, mineralization and tumor emboli were also noted. Metastasis to the regional lymph nodes, lung, liver, spleen, kidney, and adrenal gland were observed. Based on the gross and histopathologic examinations, a diagnosis of lobular type metastatic mammary gland carcinoma was made.

• Animal Systematics, Evolution and Diversity
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• 제35권2호
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• pp.49-56
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• 2019

A new marine halacarid species of the genus Agauopsis Viets, 1927 (Acari: Halacaridae) is recorded from Jeju Island, Korea. Agauopsis tetrasetosa n. sp. belongs to the microrhyncha group in sharing one ventral and three ventromedial spines on tibia of leg I, of which two ventromedial ones are adjacent to each other, with its 20 congeneric members. Agauopsis tetrasetosa n. sp. is most similar to A. miliaris Bartsch, 2005 and A. similis Bartsch, 1979 in sharing the similar chaetotaxy of legs I-IV, positions of the second and fourth dorsal setae, and non 'H'- or 'M'-shaped costae on anterior dorsal plate. However, the new species is distinguished from them by four pairs of perigenital setae in female, the relatively anterior location of gland pores on posterior dorsal plate, and three ventral spines on the tibia of leg II. We describe the new species herein, with detailed illustrations under a differential interference contrast microscope. We also make some brief comments on the affinities between the congeneric species of the microrhyncha group and the new species, based on a tabular key to species of the group.

• 한국임상수의학회지
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• 제41권4호
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• pp.234-240
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• 2024

A 9-year-old, 5.6 kg female Shih Tzu dog presented with exudate at the right eye enucleation site three months post-enucleation at the local animal hospital. Surgical removal of the periorbital tissue was immediately performed. Still, the clinical signs were not improved. Thus, the dog was referred to Chonnam National University Veterinary Medical Teaching Hospital for treatment. On physical examination, pinkish-colored viscous exudate was observed, and the Periodic acid-Schiff (PAS) staining of the exudate confirmed a leakage of saliva. Computed tomography (CT) scan images showed an indistinct margin of the right zygomatic salivary gland, leading to a suspected right zygomatic salivary gland rupture. Consequently, sialoadenectomy was planned. The surgical approach to the zygomatic salivary gland was performed along the ventral margin of the zygomatic arch without ostectomy. After dissecting the masseter muscle, the ruptured zygomatic salivary gland and the affected salivary duct were successfully removed. There were no complications, and no pain response occurred at the surgical site for three months after surgery. This report demonstrates potential complications resulting from aggressive periorbital tissue debridement following enucleation. Before surgery, it is necessary to determine the cause using PAS staining and a CT scan.

• Animal cells and systems
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• 제13권1호
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• pp.25-30
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• 2009

A new marine gastrotrich species, Ptychostomella jejuensis n. sp. belonging to the family Thaumastodermatidae, is described on the basis of the specimens from subtidal sand bottom at about 6-7 m depth of Jeju Island, South Korea. Ptychostomella jejuensis n. sp. is distinguished from its congeneric species with smooth cuticular armature by the character combination: (1) small body up to about 160 ${\mu}m$ in length; (2) presence of knob-like cephalic tentacles; (3) absence of dorsal and ventral adhesive tubes; (4) bifid pedicles; (5) pyriform copulatory organ. Under scanning electron microscopy, numerous epidermal gland openings were observed in the new species, characteristically flanking a bristle. Taxonomic accounts on the affinities, some brief remarks on the epidermal gland openings and the cooccurrence with P. orientalis Lee and Chang, 2003 are also presented with detailed illustrations and scanning electron photomicrographs.