• Title/Summary/Keyword: variety selection

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Efficient shoot regeneration using cotyledon explants and Agrobacterium tumefaciens - mediated transformation of bottle gourd (Lagenaria sicraria Standl.) (박 자엽조직을 이용한 효율적인 식물체 분화와 Agrobacterium에 의한 형질전환)

  • Kim, Soo-Yun;Ahn, Yul-Kyun;Huh, Yun-Chan;Lee, Hye-Eun;Kim, Do-Sun
    • Journal of Plant Biotechnology
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    • v.41 no.3
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    • pp.140-145
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    • 2014
  • This study were carried out for selection of proper transformation variety and development of efficient regeneration and transformation methods. The number of shoot in commercial varieties of gourd plant were 0 ~ 7.3. and fusarium wilt resistant pure lines were 2.0 ~ 6.5 per dish containing on MS medium supplemented with 3 mg/L BA. The shoot regeneration frequency of fusarium wilt resistant pure lines were wide variation on the deviation. The expression of GFP was high 67% and 100% at the co-cultivation with Agrobacterium. The effective shoot regeneration plant hormone were combination BA and 2,4-D. The number and elongation condition of shoot was good after 4 weeks change with MS medium supplemented with 1 mg/L BA. Effective callus production plant hormone were combination of 3 mg/L BA and 0.1 mg/L 2.4-D.

A Study on Effective Plan for Manpower Development of CCTV Sectors (CCTV 분야별 인력양성을 위한 효율화 방안 연구)

  • Yoo, Soonduck;Yi, Seung-Jai;Ryu, Daehyun
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.16 no.3
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    • pp.57-64
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    • 2016
  • The purpose of this research is to study, through the Delphi method, using an expert panel of 14 persons, effective workforce plans for CCTV applications. The need for professional manpower for activities related to CCTV has emerged through technological development and increased utilization of the CCTV industry sectors. Efficient workforce improvement for the CCTV sector presented in this study are in regards to student education. This study proposes an appropriate description for curriculum and related promotional information. This study should create an environment for overcoming the lack of student involvement and commitment to the environment and ensure training after qualification status after the training provided, including measures to maintain. In order to prevent employee turnover, CCTV training is necessary to actively review government support measures. This study also proposes providing a proper educational environment and supply of replacement staff members brought on by training courses. In terms of education administration bodies, we propose that education and training in CCTV management provide a variety of benefits including participation in education, adequate publicity through cooperation with relevant institutions, and introduces new educational programs. We propose providing adequate training that meets the needs of consumer, as well as education, course selection and expanded curriculum in accordance with operating options and the costs of training. In addition, senior student-selected personnel are needed to address the shortage of operators starting work-experience or training.

The effects of storytelling techniques in the sex education on sound sexual value of Middle school students - Focused on the contents of the unit of 'Understanding of Adolescents' in Technology.Home Economics Textbook in Middle School - (스토리텔링 기법을 적용한 성교육이 중학생의 건강한 성가치관 형성에 미치는 효과 - 기술.가정 교과 '청소년의 이해' 단원을 중심으로 -)

  • Kang, So-Jeong;Cho, Byung-Eun
    • Journal of Korean Home Economics Education Association
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    • v.25 no.1
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    • pp.15-36
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    • 2013
  • This study aimed to identify the effects of using storytelling techniques in sex education, in which students are encouraged to talk about their experiences with sex and love and helps them form their own sex values and decision-making skills related to sex. A five-period teaching-learning plan covering issues including safe, considerate and responsible sex was developed using the storytelling technique for 134 first-year students in 4 classes. 134 questionnaires were administrated through pre-and post-test and 38 worksheets applying a variety of storytelling activities were analyzed using content analysis. Students were found to have gained a more positive outlook on relationships and a considerate attitude towards others in sex. There was also a significant increase in items considering safe sex, including perceptions on contraception methods and selection of safe contraception methods. The students' sense of sexual responsibility was also enhanced. Results of content analysis found students to have shifted to sex focusing on love and responsibility, and to have grown a stronger sense of responsibility in their relationships. Furthermore, the respondents expressed interest in selecting proper contraception methods and held strongly negative perceptions about abortion.

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Evaluation of the Genetic Diversities and the Nutritional Values of the Tra (Pangasius hypophthalmus) and the Basa (Pangasius bocourti) Catfish Cultivated in the Mekong River Delta of Vietnam

  • Men, L.T.;Thanh, V.C.;Hirata, Y.;Yamasaki, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.5
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    • pp.671-676
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    • 2005
  • A total of 50 individual catfish, the Tra (Pangasius hypophthalmus) cultivated in either floating cages (Tra-c) or in ponds (Tra-p) and the Basa (Pangasius bocourti) raised in three floating cages, were collected in two of the Mekong Delta provinces. The caudal fin of each individual fish was used for protein electrophoresis employing the SDS-PAGE method. The one fillet sides were used as a representative sample to determine the dry matter (DM), crude protein (CP), ether extract (EE) and amino acids (AAs). The catfish oil was extracted from the belly fats, and the fatty acid (FA) composition was analyzed. There were 21 bands of the Tra and the Basa. Protein bands of the two varieties were 28.6-33.3% polymorphic, while polymorphic individuals of the Tra ranged from 80.0 to 100.0%, and the Basa was 90.0% polymorphic. The phenotypic diversity (Ho) of the Tra ranged from 1.71 to 1.80, while the Basa ranged as high as 2.14%. Diversity values (H$_{EP}$) for genetic diversity markers were equal in the Tra and the Basa. The sum of the effective number of alleles (SENA) of both varieties ranged from 3.40 to 3.83 for the Basa and the Tra, respectively. The lower values of Ho and SENA, as compared with those of the fresh water prawn (Macrobrachium equidens) in the area, would suggest that the species with the low values will become extinct due to inbreeding; the gene pools of each observed population were below a suitable threshold. Many of the differences in the nutritional values of the Tra-c, the Tra-p and the Basa were measured; their nutrient values were comparable to fishmeal or fish oil. Most of the DM, CP, and EE were higher in the Tra, especially in the Tra-c. The essential AA content, especially that of lysine, was highest in the Tra-c, next highest in the Tra-p, and lowest in the Basa. Therefore, the amino acid patterns were closer to the ideal patterns in the same sequences. In contrast, the essential FAs were concentrated in the Basa fish oil. It was found that suitable selection of parents for seed production is required to avoid inbreeding. Catfish may be valuable sources of nutrition for both humans and animals, and the differences in their nutritional values by variety and/or management must be taken into account.

Selection and Feed Value Evaluation of Wheat for Grain Feed at the paddy field in Honam Region (호남지역 논 재배에 적합한 곡실 사료용 밀 품종 선발 및 사료가치 평가)

  • Baek, Youl-Chang;Choi, Hyuck;Kim, Minseok;Park, Jong-Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.3
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    • pp.156-163
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    • 2020
  • The purpose of this study was to select the wheat varieties, among 8 domestic cultivates, that are suitable, for use as grain feed at paddy fields in the Honam region. The seeds of wheat were sown and harvested in Wanju County. Among the wheat varieties, "Jojoong" was the earliest for its heading date on April 15, and it showed the earliest day for the maturing date on May 24. The heading date of "Baegjong" was the same as that for "Keumkang" on April 18, and the maturing date was one day later than that for "Keumkang" on May 29. But there was no significant difference of heading and maturing dates among the 8 wheat varieties. The lowest number of spikes/㎡ was 513/㎡ of "Goso", and the highest number of spikes/㎡ was 974/㎡ of "Chongwoo". The spikes/㎡ and yield of "Baegjon" was 542kg/10a and it was the highest yielding variety among the 8 wheat varieties. The chemical compositions of DM (dry matter) and CP (crude protein) on rumen digestibility were not significantly different among the 8 wheat varieties. Furthermore, rumen digestibility of DM and CP, and the nutrients value were slightly higher than those of corn. The domestic 8 wheat varieties for grain feed, with their high nutrient qualities, could eventually replace imported grain feed and wheat grain feed. These 8 varieties are cost-effective feed and paddy field fodder for livestock farmers.

The Development of Diagnosis for Atopic Dermatitis by Evaluating the Expression of Skin Barrier Proteins Using a Non-Invasive Method (비침습적 피부장벽단백질 측정을 통한 아토피 피부염의 진단 및 유용성)

  • Kim, In Sik;Lee, Ji-Sook
    • Korean Journal of Clinical Laboratory Science
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    • v.49 no.4
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    • pp.395-400
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    • 2017
  • Atopic dermatitis is a chronic inflammatory skin disease caused by a variety of genetic and environmental factors, dysregulation of immunological response, as well as dysfunction of the skin barrier proteins. The purpose of this study is to develop an ELISA kit suitable for evaluating the expression of skin barrier proteins. Proteins were obtained from the skin via AriNo and D-Squame patches. The efficiency of protein collection from the skin, using the Arino patch, was shown to be more effective than using D-Squame; while the efficiency of lysis using 0.1% Triton-X100 was higher than that of other lysis solutions, including 0.1 M Tris-HCL, 0.1% Tween-20, and 5 mM KOH. Recombinant skin barrier proteins, such as filaggrin and involucrin, were produced by molecular biological methods. Monoclonal antibodies against filaggrin and involucrin were produced by immunization of mice, fusion of spleen cells and myeloma cells, as well as a selection of antibody-producing hybridoma cells. The filaggrin expression in the skin of subjects suffering from atopic dermatitis was lower than that in normal mice. Involucrin expression was not altered between normal individuals and subjects with atopic dermatitis. These findings contribute to an elucidation of the importance of the skin barrier protein expression in atopic dermatitis and the development of a diagnostic kit for atopic dermatitis.

Optimization of Processing Conditions and Selection of Optimum Species for Sweet Potato Chips (품종별 고구마 칩의 제조 조건 최적화 및 최적 품종 선정)

  • Jang, Gwi-Yeong;Li, Meishan;Lee, Sang-Hoon;Woo, Koan-Sik;Sin, Hyun-Man;Kim, Hong-Sig;Lee, Jun-Soo;Jeong, Heon-Sang
    • The Korean Journal of Food And Nutrition
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    • v.26 no.3
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    • pp.565-572
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    • 2013
  • This study was performed to optimize the processing conditions and to select the optimum species for colored sweet potato chips (Shinjami, Juhwangmi and Hayanmi varieties) baked by far-infrared radiation. Sweet potato chips were prepared by different slicing thickness (1~3 mm), concentration of sucrose solution for soaking (15~30%), blanching time (15~60 sec), baking temperature ($110{\sim}140^{\circ}C$) and baking time (23~31 min) through sensory evaluation and failure stress measurement. Optimal processing condition of sweet potato chips using Shinjami was determined to 1 mm, 20%, 45 sec, $120^{\circ}C$ and 31 min, and those using Juhwangmi was determined as 1 mm, 25%, 45 sec, $130^{\circ}C$ and 29 min. Sweet potato chips using Hayanmi was determined as 1 mm, 20%, 45 sec, $120^{\circ}C$ and 31 min, respectively. Free sugar content of sweet potato chips was higher in chips than in raw materials. In the sensory evaluation, appearance, sweet taste, hardness, and overall acceptance were higher in Juhwangmi than in the Shinjami and Hayanmi varieties. Overall acceptance, sugar content, b-value and failure stress were highly correlated among factors affecting the sensory characteristics (p<0.01). From the results of this study, Juhwangmi variety was selected for production of sweet potato chips.

Dietary Attitude of Single Households in Metropolitan Areas (수도권에 거주하는 1인 가구 식생활 태도 조사)

  • Heo, Yun Kyung;Sim, Ki Hyeon
    • The Korean Journal of Food And Nutrition
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    • v.29 no.5
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    • pp.735-745
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    • 2016
  • This study conducted a survey with 203 single households among men and women in their 20s to 40s who were living in metropolitan areas from October 6 to November 4, 2012 in order to investigate the dietary attitude of the single households. The ratio of single households who had three meals a day regularly was 2.85 points, which was lower than the normal level, and it turned out that those in their 20s and 30s had meals more regularly than those in their 40s did (p<0.001). As for the irregular meal time, most were breakfast (85.9%), and it turned out that they often skipped meals mostly because they did not have time to eat and (41.7%) or because that bothered them (26.0%). 62.6% of the single households did overeating and most of them (39.4%) did overeating because of their irregular meals. Of the single households, women or persons who had lived alone for less than 3 years or more than 7 years cooked at home, more often (p<0.05), and most of them (42%) cooked noodles, easy to cook, but women cooked Korean food-based homemade food such as rice (31.7%) or soup and stew (21.2%), often (p<0.05). It turned out that 36.9% of the single households often ate out about two to three times a day, and as for their favorite eating-out menus, 39.4% were Korean food, followed by Western food (23.8%), flour-based food (13.5%), fast food (9.8%), Chinese food (7.3%) and Japanese food (6.2%). Lastly, as for inconveniences when they ate out, most were the 'price' (22.8%), followed by 'too much amount of food for one person' (20.2%) and 'limitations in menu selection' (19.2%). As a result of this study, it appeared that the single households had an irregular dietary life, often did overeating and often ate out, so it is judged that it would be necessary to develop a variety of nutritionally-balanced HMR food and eating-out menus in a reasonable price range for their healthy dietary life.

Selection of Optimal Vegetation Indices for Estimation of Barley & Wheat Growth based on Remote Sensing - An Application of Unmanned Aerial Vehicle and Field Investigation Data - (원격탐사 기반 맥류 작황 추정을 위한 최적 식생지수 선정 - UAV와 현장 측정자료를 활용하여 -)

  • Na, Sang-il;Park, Chan-won;Cheong, Young-kuen;Kang, Chon-sik;Choi, In-bae;Lee, Kyung-do
    • Korean Journal of Remote Sensing
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    • v.32 no.5
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    • pp.483-497
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    • 2016
  • Unmanned Aerial Vehicle (UAV) imagery are being assessed for analyzing within field spatial variability for agricultural precision management, because UAV imagery may be acquired quickly during critical periods of rapid crop growth. This study refers to the derivation of barley and wheat growth prediction equation by using UAV derived vegetation index. UAV imagery was taken on the test plots six times from late February to late June during the barley and wheat growing season. The field spectral reflectance during growing period for the 5 variety (Keunal-bori, Huinchalssal-bori, Saechalssal-bori, Keumkang and Jopum) were measured using ground spectroradiometer and three growth parameters, including plant height, shoot dry weight and number of tiller were investigated for each ground survey. Among the 6 Vegetation Indices (VI), the RVI, NDVI, NGRDI and GLI between measured and image derived showed high relationship with the coefficient of determination respectively. Using the field investigation data, the vegetation indices regression curves were derived, and the growth parameters were tried to compare with the VIs value.

Selection of Superior Sorghum Accession by Assessing Agronomic Characters and Biological Activity (수수 수집종의 농업적 형질 및 생리활성 분석을 통한 우수계통 선발)

  • Jeon, Mi Ran;Yoo, Ji Hye;Kim, Chang Heum;Choi, Jae Hoo;Kang, Byeong Ju;Seong, Eun Soo;Yu, Chang Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.5
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    • pp.386-392
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    • 2016
  • Background: Sorghum is a major cereal food crop used in many parts of the world. It has been grown on a subsistence level by farmers, under various conditions of environmental stresses in the semi-arid tropics of Africa and Asia. This plant has received significant attention because of its ability to reduce cholesterol in the blood, and its anti-dementia, antioxidant, and antimicrobial properties. It is possible to develop a functional and commercially viable sorghum variety by using superior cultivars of sorghum. The objective of this study was to build a database of superior sorghum accession. Methods and Results: We used 250 sorghum accessions collected from different geographical bioregions in Korea. We determined various agronomic characters including germination rate and ear length of these accessions. To determine the antioxidant capacity, we measured the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt radical scavenging activity, total phenolic contents, and total flavonoid contents. Accession 189 showed higher germination (> 80%) than the other accessions. Higher 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was observed in 11-SB-078 ($RC_{50}$; $1.89{\pm}2.88{\mu}g/m{\ell}$), and higher 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)-diammonium salt radical scavenging activity was recorded in 11-SB-116 ($RC_{50}$; $35.48{\pm}2.42{\mu}g/m{\ell}$) than in the other accessions. The ear length ranged from 15 cm to 48 cm, the total phenolic contents ranged from $3mg{\cdot}GAE/g$ to $77mg{\cdot}GAE/g$, and total flavonoid contents ranged from $0.09mg{\cdot}QE/g$ to $1.07mg{\cdot}QE/g$. Conclusions: Among 250 sorghum accessions, we selected 10 with both superior agronomic characters and highly functional food quality.