• 한국자원식물학회지
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• 제26권6호
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• pp.673-677
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• 2013

In order to examine the effects of Korean cucurbitaceous plants on sonic hedgehog pathway and growth of cancer cells with over-activated hedgehog pathway, we measured the sonic hedgehog conditioned medium (shh-CM) induced alkaline phosphatase (ALP) activity and cell viability of pancreatic cancer cell lines by treatment of cucurbitaceous plants. Among the tested cucurbitaceous plants, Actinostemma lobatum Maxim, Cucumis sativus L., Momordica charantia L., Schizopepon bryoniaefolius Maxim and Trichosanthes kirilowii Max, var. japonica Kitam showed the potent inhibitory effects (> 50 % at $20{\mu}g/mL$) on shh-CM induced ALP activity. We also evaluated the cell viability of pancreatic cancer cells treated with the cucurbitaceous plants. The tested cucurbitaceous plants showed the very weak effects on cancer cell proliferation but, T. kirilowii Max, var. japonica Kitam presented the inhibitory effect of 72.7 % on the proliferation of pancreatic cancer cells at $20{\mu}g/mL$. Taken together, we screened the effects of Korean cucurbitaceous plants on shh-CM induced ALP activity and cell viability of pancreatic cancers to search for the modulators of the hedgehog pathway leading to the inhibition of cancer cell proliferation. T. kirilowii Max, var. japonica Kitam, among the tested cucurbitaceous plants, showed the inhibitory effects on the shh-CM induced ALP activity and the proliferation of pancreatic cancer cells.

• 한국약용작물학회지
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• 제22권1호
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• pp.53-59
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• 2014

This study was conducted to determine the effects of seed storage temperature and pre-treatment on seed germination, seedling quality and vigor index of wild Trichosanthes kirilowii and Trichosanthes kirilowii var. japonica. As a result the highest germination rate of T. kirilowii was the 95% at seed stored in room temperature and then soaking for 24 hours in $dH_2O$. And the highest germination rate of T. kirilowii var. japonica was 96% at seed stored in $2^{\circ}C$ and then soaking for 24 hours in $GA_3$ (100 ppm). But the seed germination rate was non-significance in pre-treatment at seed stored in room temperature $2^{\circ}C$. Seedlings of T. kirilowii and T. kirilowii var. japonica showed not only the best seedling quality but also seedling vigor index in seed stored at $2^{\circ}C$ and then soaking for 24 hours in $GA_3$ (100 ppm). In this research, T. kirilowii and T. kirilowii var. japonica seed were stored in room temperature or $2^{\circ}C$, and then sown in peat moss, seed germination rate was more than 90% and production of superior quality seedlings.

• 식물분류학회지
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• 제42권1호
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• pp.40-49
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• 2012

동북아산 미역취 복합체(S. virgaurea complex)에는 미역취(S. virgaurea subsp. asiatica), 잔미역취(S. virgaurea var. taquetii), 나래미역취(S. virgaurea var. coreana), 산미역취(S. virgaurea subsp. leiocarpa), 울릉미역취(S. virgaurea subsp. gigantea) 등 5가지의 종하분류군이 보고되고 있으나, 분류군의 실체 및 한계가 명확히 구분되지 않고 있다. 따라서 본 연구에서는 원기재문과 기준표본을 바탕으로 국내의 9개 자생지 집단을 조사하여 미역취의 외부형태학적 형질을 재검토하였고, 동북아산 미역취 복합체의 실체 확인 및 분류학적 처리를 수행하였다. 또한 문헌 및 표본 관찰을 통해 동아시아산 미역취속의 분포역을 파악하였다. 그 결과, 미역취는 유럽산 미역취(S. virgaurea L.)와 명확히 구분되는 독립된 종(S. japonica Kitam.)으로 인정되었으며, 울릉도를 제외한 한반도 전역과 일본에만 한정 분포하는 것으로 조사되었다. 그리고 잔미역취와 나래미역취의 검색형질들은 미역취의 해당 형질 변이 폭 내에 포함되므로 모두 미역취의 이명으로 처리하였으며, 산미역취는 국내에 분포하지 않는 것으로 확인되었다. 한편 울릉미역취는 충분한 실험재료가 확보되지 않았기 때문에 분류학적 처리를 보류하였으며, 두메미역취가 한반도 북부에 자생하는 것으로 확인되었다.