• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.21 no.2
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• pp.39-45
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• 2008

Objective : Skin barrier protects skin against harmful environment. Its function includes antimicrobial barrier as well as physical barrier. But there are few studies about the factors which improve skin barrier recovery after injury. The aim of this study is to investigate the factors which improve skin barrier recovery. Method : Nine hairless mice was anesthetized by inhalation and we tape stripped them. We used thermometer to know temperature one day ago, before anesthesia, before tape stripping, and after tape stripping. Vapometer was used to know transepidermal water loss before tape stripping, after tape stripping. And we used doppler flowmeter to measure blood flow before tape stripping, after tape stripping. Result : After analyzed data, we concluded that temperature was lower after anesthesia than before anesthesia and after tape stripping than before tape stripping. We could know that transepidermal water loss was lower after tape stripping than before tape stripping and more blood flowed after tape stripping. Conclusion : In our study, it was observed that temperature, transepidermal water loss, blood flow changed after tape stripping. But we thought lowered temperature was pathologic situation, more blood flow was to recover after injury. In traditional korean medicine, cold(寒) and imbalance of blood flow(血行) don't only make many skin diseases, but cold(寒) also obstructs blood flow. So we need to study how to warm cold(寒) and improve blood flow.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.20 no.2 s.33
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• pp.179-186
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• 2007

Objective : Skin barrier protects skin against harmful environment. Its function includes antimicrobial barrier as well as physical barrier. But there are few studies about skin barrier function about men and women who don't have skin disease. The aim of this study is to investigate skin barrier function healthy men and women show. Method : There are many ways to measure skin barrier function, but lately transepidermal water loss is mainly used to know skin barrier function. Transepidermal water loss was measured over eight four the Korean twenties and thirties who don't have skin disease from March 14th, 2007 to March 16th 2007. We surveyed cubital fossa and cheek beside nose to measure transepidermal water loss and used vapometer. Result : After analyzed data, we concluded that cheek showed higher transepidermal water loss than cuital fossa. At cheek, the thirties had higher transepidermal water loss than the twenties. The male got higher transepidermal water loss than the female at cheek and in the average. And temperature and humidity effected on transepidermal water loss. Conclusion: In our study, it was observed that temperature and humidity effected on transepidermal water loss. In traditional korean medicine, the inbalance of cold(寒), heat(熱), dryness(燥), wetness(濕) makes many diseases and the inbalance of them induces dameum(痰飮) and the disorder of blood flow. Dameum(痰飮) and the disorder of blood flow make skin diseases made by abnormal skin barrier function in many ways. So we need to study dameum(痰飮) and the disorder of blood flow to take care of patients who have abnormal skin barrier function.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.38 no.1
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• pp.43-50
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• 2012

The safety of cosmetics or cosmetic ingredients on human skin is generally evaluated by visual assessment but some early subtle skin changes may not be noticed by the naked eyes. Thus, the present study was conducted to detect skin reactions induced by mildly irritating cosmetic ingredients by using a laser Doppler perfusion imager (LDPI) method that measures blood flow, a $Vapometer^{(R)}$ that measure strans epidermal water loss (TEWL), and a spectrophotometer that measures the skin color as the erythema values ($a^*$). Visual assessment showed that all tested oils and humectants except propylene glycol belong to the low skin irritation ranges (grades 0+ to 2.9+) while all tested surfactants and propylene glycol belong to the moderate-to strong-skin irritation ranges (grades 3+ to 5+). Among three instrumental methods, TEWL assessment appeared to be more sensitive than spectrophotometric or LDPI method and suitable for the detection of subtle skin response invisible to the naked eye (grades 0+ to 2.9+). Skin reactions of grade 3+ to 5+ could be detected by all three instrumental methods. In conclusion, the current study suggested that the sub-clinical skin reactions due to mild irritants contained in cosmetics can be best assessed by TEWL measurements.

• Journal of the Korean Applied Science and Technology
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• v.40 no.5
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• pp.936-944
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• 2023

The purpose of this study is to verify the effect of essence containing Coptis Japonica extract on skin barrier strengthening and moisturizing. The essence containing the Coptis Japonica extract was used by 22 subjects for four weeks, and the amount of percutaneous water loss (g/m²h) and water (A.U.) in the designated upper limit were measured. As a result of measuring percutaneous water loss using a vapometer, it was found to be 11.08±2.55 after 4 weeks of use from 14.82±4.01 before use, and the significance probability decreased significantly by 25.27% to p<0.001. The amount of skin moisture measured by the Corneometer was found to be 46.73±7.45 after use from 31.73±8.09 before use, and the significance probability increased by 47.26% to p<0.001, and the subject's subjective efficacy evaluation through a questionnaire showed that all subjects were 100% satisfied without skin adverse reactions. As a result of this, it has been confirmed that Coptis Japonica essence is effective in strengthening skin barriers and moisturizing, which is expected to be a cornerstone for the development of cosmetics using various activities of Coptis Japonica extract in the future.

• Journal of Life Science
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• v.33 no.11
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• pp.905-914
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• 2023

To evaluate the effectiveness of the skin barrier improvement of lactic acid (LA) and gluconolactone (GL), the expression of filaggrin, loricrin, hyaluronic acid (HA), hyaluronan syhthase-2 (HAS2), and aquaporine-3 (AQP3) in keratinocytes, and the moisture content and transepidermal water loss (TEWL) by clinical trials were evaluated. The expression levels of filaggrin and locricrin, which are the main factors affecting the proper functioning of skin barrier function, and HA, HAS2, and AQP3, which are skin moisturizing-related proteins measured by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting. The results showed that the expression levels of the factors that decreased by H2O2 treatment were significantly increased by LA, GL, and a mixture of LA and GL at the mRNA and protein levels (p<0.05). The nanoemulsion containing a mixture of LA and GL was prepared using the emulsion inversion method, and the average particle size was 299.9 ± 0.287 nm. After measuring the TEWL of nanoemulsion using Vapometer, it was found that TEWL significantly decreased by 15.53% and 26.73% after two weeks and four weeks of product use, respectively, compared to TEWL before product use (p<0.001). Similarly, the skin moisture content of the nanoemulsion significantly increased by 15.40% and 26.59% after two weeks and four weeks of product use, respectively, compared to skin moisture content before product use (p<0.001). Therefore, the skin barrier function and moisturizing effect of a mixture of LA and GL are shown by increasing the moisture content and decreasing the TEWL by increasing the expression of filaggrin, loricrin, HA, HAS2, and AQP3. This suggests the possibility for the development of functional cosmetic ingredients in the future.