• Preventive Nutrition and Food Science
• /
• v.22 no.1
• /
• pp.50-55
• /
• 2017

Raphanus sativus L. (RS) is a cruciferous vegetable that is widely consumed in Korea. The anticancer activity of leaves of RS (RSL) extract has been investigated; however, no studies focused on its anti-inflammatory effects. Therefore, the aim of the current study was to evaluate the anti-inflammatory effects of RSL extract. In brief, RSL powder was fractionated into n-hexane, chloroform, ethyl acetate, n-butanol, and water-soluble fractions. Lipopolysaccharide (LPS)-stimulated RAW264.7 cells were treated with each fraction for initial screening. It was found that the chloroform fraction significantly inhibited nitric oxide release in LPS-stimulated RAW264.7 cells with a half maximal inhibitory concentration value of $196{\mu}g/mL$. In addition, the mRNA and protein expression levels of inducible nitric oxide synthase, measured using reverse transcriptase-polymerase chain reaction and western blotting, respectively, were reduced in a concentration-dependent manner. Moreover, the inflammatory cyclooxygenase-2 enzyme expression decreased. Furthermore, the expression of nuclear factor-kappa B ($NF-{\kappa}B$), the key regulator of the transcriptional activation of the inflammatory cytokine genes, was reduced by the RSL chloroform fraction. Therefore, the results of our study suggest that RSL exhibits anti-inflammatory effects in LPS-stimulated macrophages via $NF-{\kappa}B$ inactivation.

• KSBB Journal
• /
• v.5 no.2
• /
• pp.183-189
• /
• 1990

An efficient method has been developed for the purification of urokinase from 1, 000 liter batches of human urine. The procedure involved precipitation of urokinase with 2mM zinc chloride, resuspension of the precipitate with 0.1M EDTA/0.5M Glycine solution, and CM-Toyopearl and benzamidine-Sepharose column chromatography. The purified urokinase was fully active and possessed a specific activity of 1.07$\times$105IU/mg. The recoveries ranged from 42 to 65% in several preparations(mean value was 51%). And the urokinase purified by this process consisted of about 13% of single chain urokinase (pro-urokinase) as evaluated by SDS-polyacrylamide gel electrophoresis in reducing condition and by S-2444 amldolytic activity under plasmin treatment.

• Proceedings of the Safety Management and Science Conference
• /
• 2003.11a
• /
• pp.105-111
• /
• 2003

This paper examines to focus on General Strategy of e-logistics activity for SCM(Supply Chain Management) Build-up, especially the field service logistics Integration. And this research analyzes the case study (Business of Strategy Group Unit) Survey) involving effective logistics management that leads customer satisfaction, seeking to find out the key elements of competitive policy. Because problems arise from the differences between their goals and structures, which cause each sied to feel vulneralbe to opportunism or shirking of responsibilities by the other. The goal of a logistics system is to ensure that every family planning client always receives the contraceptives partner wants, and that they are in good condition and have not expired. In order to select, procure, and distribute the right Quantities of the right contraceptives, managers of the logistics system must keep informed of the rates of use of each contraceptive and of the Quantity of available supplies. As a result, We propose that a firm's e-logistics strategy is an important locus innovation and a crucial source of value creation for the firm its suppliers, partners, and customers.

• Journal of the Korea Safety Management & Science
• /
• v.5 no.4
• /
• pp.117-130
• /
• 2003

This paper examines to focus on General Strategy of e-logistics activity for SCM(Supply Chain Management) Build-up, especially the field service logistics Integration. And this research analyzes the case study(Business of Strategy Group Unit) Survey) involving effective logistics management that leads customer satisfaction, seeking to find out the key elements of competitive policy. Because problems arise from the differences between their goals and structures, which cause each sied to feel vulneralbe to opportunism or shirking of responsibilities by the other. The goal of a logistics system is to ensure that every family planning client always receives the contraceptives partner wants, and that they are in good condition and have not expired. In order to select, procure, and distribute the right quantities of the right contraceptives, managers of the logistics system must keep informed of the rates of use of each contraceptive and of the quantity of available supplies. As a result, We propose that a firm's e-logistics strategy is an important locus innovation and a crucial source of value creation for the firm its suppliers, partners, and customers.

• Microbiology and Biotechnology Letters
• /
• v.20 no.1
• /
• pp.25-33
• /
• 1992

One acetolactate synthase isozyme which has Rf value of 0.83 on polyacrylamide gel electrophoresis was purified from Sewatia marcescens ATCC 25419 by ammonium sulfate fractionation, DEAE-Sephacel chromatography, Phenyl-Sepharose chromatography, Sephacryt S-400 gel filtration followed by native gel elution. The native molecular weight of the enzyme was determined to be 531,400 by gel filtration method, and SDS-polyacrylamide gel electrophoresis separated the native enzyme into two polypeptides having molecular sizes of 55,000 and 38,900 respectively. In kinetic parameters, $K_m$ value for pyruvate was 2.54 mM, and $V_{max}$ was 21.75 nmoie/min/mg. The enzyme showed maximal activity around pH 8.0 and optimal temperature of the acetolactate formation was $37^{\circ}C$. Feedback inhibition studies indicate that the purified enzyme is rather resistant to branched chain amino acids when compared with acetolactate synthase isozymes of plants or other enterobacteria.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.29 no.2
• /
• pp.286-297
• /
• 2005

Recently, due to the gloalization of the industry environment and the consequent multi-national companies led by industrialized countries, fashion companies of newly industrialized countries are pursuing to globalize their businesses. This study conducted to review the global configuration of activities of the added value chain, the strategies to enhance global competitiveness, and global performance of value activities of Korean fashion companies. The data were collected from the survey by questionnaire on Korean fashion companies. The methods of analysis used in this study were regression, and descriptive statistics of the data. the results werw as follows. First, Korean fashion companies were undergoing diverse value activities through their foreign subsidiaries and overseas out-sourced companies in a wide spectrum of business activities ranging from design/planning o products to marketing/logistics. Second, the global competitive strategies that the Korean fashion companies were most focused on were the cost/productivity aspects. The efforts to differentiate the products from competitors, the efforts for innovation, an the efforts of growth were the next strategies. The strategy that was least emphasized was on seeking alliance with other business partners. Third, the result of the globalization efforts showed an overseas market growth of and average of 9%, and an average overseas market profit of 7.8%. The overall satisfaction on the result of the overseas market activities, and the rate of accomplishment of market targets were generally low.

• International conference on construction engineering and project management
• /
• 2005.10a
• /
• pp.1089-1093
• /
• 2005

Proving the effect of lean construction has yet to be done for its staying poor basic building though there has been efforts and wants to introduce the theory and concept of lean construction in practical site. Therefore, we need to specify the methods of productivity improvement and construction process through various adaptations in the site. Also, to apply such improvement methods systematically, we need active participation of owners, contractors and supply chain members in relation to construction industry and are also require appropriate methods to adapt. At first, this study thinks over the techniques of lean construction that has been verified through studies carried before. For second, it selects the technique of lean construction with the practicality in the construction site. For third, it is aimed to make a manual of lean process management by the use of TACT and V.S.M. For fifth, it will show the method of management for repeated activity and develop a guide-line and procedure that applies the concept of work sequence and resource equalization to each phase of process management. At last, it will show methods of reflecting V.S.M. to improve and grasp the waste factor from the step of managing an activity process and develop the form to analyze the waste in construction site by mapping the value flow.

• Microbiology and Biotechnology Letters
• /
• v.19 no.5
• /
• pp.470-476
• /
• 1991

The distribution of peroxidase activity in 9 kinds of cruciferous plants was investigated. Among the plants examined, peroxidase activity was found to be high levels in roots of Chinese cabbage. One kind of peroxidase was purified approximately 56-fold from crude extracts of Chinese cabbage roots. The molecular weight of the enzyme was 50, 000 and consisted oif a single polypeptide chain, as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Sephadex G-150 gel column chromatography. The enzyme showed optimum activity at pH 7.0 and $50^{\circ}C$. Phenol and phenol derivatives serves as substrates of the enzyme and Km value for $H_2O_2$ was 1.6 mM toward pyrogallol. The enzyme showed a Soret band at 406 nm and this result indicate that the enzyme contained heme as a prosthetic group. The immunochemical and electrophoretic properties of purified peroxidase from Chinese cabbage were very similar to horseradish peroxidase.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.7
• /
• pp.898-904
• /
• 2014

The stability of Bacillus licheniformis alpha-amylase (BLA) under acid condition was enhanced through direct evolution using the error-prone polymerase chain reaction. One beneficial mutation site, H281I, was obtained in BLA. The specific activity of H281I was 161/352 U/mg, which was 62.6/27.5% higher than that of the wild-type (WT) (99/276 U/mg) at pH 4.5/6.5 and $95^{\circ}C$. The pH optimum for H281I was decreased about 1 unit, whereas no significant changes of optimum temperature and thermostability were observed compared with the wild type (WT). The $k_{cat}/K_m$ value of H281I was 1.7-/1.4-fold higher at pH 4.5/6.5, respectively, than that of WT. The structure model analysis indicated that the H281I mutation altered the predicted interaction between the amino acid residues at 281 and 273, thus creating a conducive local environment for substrate binding, as reflected by its decreased $K_m$, and consequently increased the specific activity.

• Journal of Microbiology and Biotechnology
• /
• v.23 no.2
• /
• pp.251-259
• /
• 2013

An endoxylanase gene (PoxynA) that belongs to the glycoside hydrolase (GH) family 11 was cloned from a xylanolytic strain, Penicillium oxalicum B3-11(2). PoxynA was overexpressed in Trichoderma reesei QM9414 by using a constitutive strong promoter of the encoding pyruvate decarboxylase (pdc). The high extracellular xylanase activities in the fermentation liquid of the transformants were maintained 29~35-fold higher compared with the wild strain. The recombinant POXYNA was purified to homogeneity, and its characters were analyzed. Its optimal temperature and pH value were $50^{\circ}C$ and 5.0, respectively. The enzyme was stable at a pH range of 2.0 to 7.0. Using beechwood as the substrate, POXYNA had a high specific activity of $1,856{\pm}53.5$ IU/mg. In the presence of metal ions, such as $Cu^{2+}$, and $Mg^{2+}$, the activity of the enzyme increased. However, strong inhibition of the enzyme activity was observed in the presence of $Mn^{2+}$ and $Fe^{2+}$. The recombinant POXYNA hydrolyzed birchwood xylan, beechwood xylan, and oat spelt xylan to produce short-chain xylooligosaccharides, xylopentaose, xylotriose, and xylobiose as the main products. This is the first report on the expression properties of a recombinant endoxylanase gene from Penicillium oxalicum. The properties of this endoxylanase make it promising for applications in the food and feed industries.