• Clinical and Experimental Reproductive Medicine
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• 제21권2호
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• pp.183-190
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• 1994

Purpose of the present study was to find the optimal culture conditions for the maturation and fertilization of immature oocytes by the use human body fluids and gonadotropins (Gn) in the mouse model. Cumulus-enclosed mouse immature oocytes were incubated in the medium containing various human body fluids with or without Gn in vitro, and examined to confirm nuclear maturation (NM) and fertilization. Female ICR mice were stimulated with 7.5 IU pregnant mares' serum gonadotropin (PMSG). Cumulus-enclosed immature oocytes were isolated at 48-52 hr post PMSG injection and cultured in TCM 199 supplemented with various concentrations (20, 50, and 70%) of human body fluids such as fetal cord serum (hCS), follicular fluid (hFF), peritoneal fluid (hPF) and amniotic fluid (hAF) in the presence or absence of 10 IU/ml PMSG and 10 IU/ml human chorionic gonadotropin (hCG) for 18 hr. Fetal calf serum (FCS) was used as a control for the supplements. Matured oocytes were fertilized with sperm collected from the epididymis of male mice. Fertilization was conducted in T6 medium containing 15 mgl ml bovine serum albumin, and confirmed at 6 hr post-insemination. Evaluation of nucler maturation and fertilization was carried out by rapid staining using fuchin. There was no significant difference between the effects of human body fluids and FCS supplements on nuclear maturation of cumulus enclosed mouse immature oocytes. When maturation medium was supplemented with 20% hPF or 20% hAF, fertilization rates were significantly (P<0.01) lower than that of 20% FCS, hCS and hFF groups. However, higher concentrations of body fluids during IVM were not more beneficial on fertilizability of oocytes. The addition of Gn significantly increased the fertilization rates in hPF and hAF groups (hPF without Gn; 51.5%, compared with 85.1% for addition of Gn, and hAF without Gn; 30.1% compared with 85.8% for addition of Gn) at 20% concentration. These results suggest that human body fluids at 20% concentration and gonadotropins can be used as supplements for the maturation of mouse immature oocytes in vitro. When gonadotropins supplemented with the human body fluids in the maturation medium, fertilizability of mouse immature oocytes was increased in hPF and hAF groups. These results can be applied to maturation of human immature oocytes in vitro.

• Wind and Structures
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• 제20권4호
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• pp.549-564
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• 2015

The three-dimensional unsteady incompressible Reynolds-averaged Navier-Stokes equations and k-${\varepsilon}$ double equations turbulent model were used to investigate the effect on the measurements of anemometers due to a passing high-speed train. Sliding mesh technology in Fluent was utilized to treat the moving boundary problem. The high-speed train considered in this paper was with bogies and inter-carriage gaps. Combined with the results of the wind tunnel test in a published paper, the accuracy of the present numerical method was validated to be used for further study. In addition, the difference of slipstream between three-car and eight-car grouping models was analyzed, and a series of numerical simulations were carried out to study the influences of the anemometer heights, the train speeds, the crosswind speeds and the directions of the induced slipstream on the measurements of the anemometers. The results show that the influence factors of the train-induced slipstream are the passing head car and tail car. Using the three-car grouping model to analyze the train-induced flow is reasonable. The maxima of horizontal slipstream velocity tend to reduce as the height of the anemometer increases. With the train speed increasing, the relationship between $V_{train}$ and $V_{induced\;slipstream}$ can be expressed with linear increment. In the absence of natural wind conditions, from the head car arriving to the tail car leaving, the induced wind direction changes about $330^{\circ}$, while under the crosswind condition the wind direction fluctuates around $-90^{\circ}$. With the crosswind speed increasing, the peaks of $V_X,{\mid}V_{XY}-V_{wind}{\mid}$ of the head car and that of $V_X$ of the tail car tend to enlarge. Thus, when anemometers are installed along high-speed railways, it is important to study the effect on the measurements of anemometers due to the train-induced slipstream.

• Asian Pacific Journal of Cancer Prevention
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• 제16권15호
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• pp.6463-6468
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• 2015

Background: Chemotherapy is one of the common approaches in treatment of cancers, especially leukemia. However, drug resistance phenomena reduce the likelihood of treatment success. Resveratrol is a herbal compound which through complicated processes makes some selected cells sensitive to treatment and induction of apoptosis. In the present study, the effects of resveratrol on the expression of miR 15a and miR16-1 and apoptosis in the CCRF-CEM cell line were investigated. Materials and Methods: The CCRF-CEM cell line was cultured under standard conditions and changes in miR 15a and miR 16-1 expression were analyzed by real time-PCR technique, with attention to reveratrol dose and time dependence. Also, apoptosis is evaluated by flow cytometry using annexin V and PI. Results: CCRF-CEM cells underwent dose-dependent apoptotic cell death in response to resveratrol. MiR 15a and miR 16-1 expression was up-regulated after 24 and 48 hours resveratrol treatment (p<0.05). Conclusions: The results of our study indicate that resveratrol induces apoptosis in a time and dose-dependent manner in CCRF-CEM cells. Also, increased expression level of miR 16-1 and miR 15a by means of resveratrol in CCRF-CEM cells might have a role in apoptosis induction and predisposition. According to our results resveratrol can be regarded as a dietary supplement to improve efficacy of anti-leukemia therapies.

• 생태와환경
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• 제41권3호
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• pp.367-373
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• 2008

유해남조의 생물학적 제어를 위한 연구의 일환으로 북한강수계(가평)에 서식하는 이매패 말조개(Unio douglasiae)의 섭식특성을 조사하였다. 섭식실험은 크게 시간별, 개체크기, 동물밀도, 먹이밀도 (엽록소-a 농도) 등에 따라 패류의 여과율 및 배설물 생산량을 각각 측정하였다. 말조개의 여과율은 섭식 후 적용 7시간째 (0.21 L $g^{-1}h^{-1}$, 0.54 mg $g^{-1}h^{-1}$), 크기 5 cm보다 작은 개체에서 최고 여과율 및 배설물 생산량 (5.89 L $g^{-1}h^{-1}$, 16.38 mg $g^{-1}h^{-1}$)을 각각 나타냈다. 또한 가장 낮은 먹이밀도 (엽록소-a 88.5 ${\mu}gL^{-1}$)에서 0.41 L $g^{-1}h^{-1}$로 최고 여과율을 보였으며, 일정 먹이농도 이상에서는 배설물 생산량이 증가하지 않는 것으로 나타났다. 또한 패류의 조류 제어효과는 개체 밀도가 증가할수록 뚜렷한 효과를 나타냈다. 결국 이상의 결과들은 북한강에 서식하는 이매패 말조개가 부영양호수의 남조류 제어의 유용생물로서 적용 가능성을 입증하였다.

• 핵의학기술
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• 제16권2호
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• pp.106-109
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• 2012

최근 늘어나는 [$^{18}F$]FDG-PET 검사 증대와 더불어 새로운 방사성의약품으로 [$^{11}C$]아세테이트 검사가 신설되고 다양한 연구용 $^{11}C$-표지 방사성의약품 이용이 증대되고 있다. 본 연구에서는 성공리에 수행한 한국형 사이클로트론의 $^{11}C$-표적시스템을 이용하여, $[^{11}C]CO_2$ 생산 최적화 및 임상에서 사용가능한 $^{11}C$-표지 방사성의약품 생산 적용 연구를 수행하였다.

• 한국지반공학회지:지반
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• 제14권5호
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• pp.39-52
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• 1998

본 논문은 정규압밀된 점성토 지반에서 강관 매입 말뚝의 수평 거동에 영향을 미치는 여러인자들 중에서 말뚝의 근입길이, 지반조건(건조단위 중량 $\gamma_4$), 말뚝 두부의 구속조건의 영향에 관하여 모형실험을 수행하였으며, 이러한 영향을 정량화 할 수 있는 경첩식을 그 결과들로부터 얻었다. 모형실험에 사용한 지반은 3종류의 정규압밀 점토이다. 2종류의 말뚝 근입길이와 말뚝 두부의 고정 자유조건의 모형실험결과들에 의하여 수평하중-변위 관계는 $\gamma_d/\gamma_{dmax}$=0.84 이하에서 완전 탄소성체의 거동형상을 보일 것으로 나타났으며, 각 실험에서 최대 수평하중(Q--) 이후의 수평하중 감소는 상당한 시간 의존성을 보였다. 본 연구에서는 수평 하중-변위관계(logo-logy/D)와 최대 휨 모멘트-변위관계($loaM_{max}$.-.-logy/D)에서 각각 구한 항복 수평하중(Qy)과 항복 최대 휭 모멘트(My)가 직선적인 관계로 밝혀졌다. Relaxation에 의한 수평하 중은 모형실험 결과들로부터 시간을 변수로 한 지수 함수식으로 회귀분석 하였다. 수평 극한하중과 항복하중에 대한 지반조건의 영향은 $\gamma_4$$/\gamma_{dmax}$의 변수로 한 지수함수식으로 모델화 하였으며, Broms와 Budhu et at.에 의한 결과와의 비교에서 예측결과가 26-78%정도 과대평가 되었다. 수평하중-변위 관계에 대한 말뚝 두부의 고정조건 영향에서 $Q_{mxed}/Q_{free}$-y/D의 관계는 상당히 비선형적으로 나타났으며, yiD를 변수로 한 지수함수식으로 모델화하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권14호
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• pp.5773-5777
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• 2015

Background: Oral squamous cell carcinoma (OSCC) is thought to develop from precancerous dysplastic lesions through multistep processes of carcinogenesis involving activation of oncogenes and loss of tumor suppressor genes. The human epidermal growth factor receptor 2 (Her-2/neu [erbB-2]), a cell membrane glycoprotein, is a growth factor receptor that has receptor tyrosine kinase activity. Her2/neu activation plays a central role in cell proliferation and survival. It has been shown that overexpression of Her2/neu increases the rate of cell division and growth, leading to precancerous changes. The aim of the present study was to compare the serum and salivary Her2/neu levels between cases with premalignant and malignant oral lesions. Materials and Methods: Fasting blood samples and unstimulated saliva by passive drooling were collected from three groups of healthy control (n=20), premalignant disorder (PMD) (n=20) and OSCC (n=25) subjects. The HER2 extracellular domain (HER2 ECD) levels were measured using ELISA. Results: The levels of serum Her2/neu showed no significant differences between any of the groups but on the other hand salivary Her2/neu levels were found to be significantly (p<0.05) higher when compared between control (median 68.7 pg/ml, range: 21.5 - 75.8) and OSCC (median 145.6 pg/ml, range: 45.1-191.1). A similar trend was observed when comparing between PMD (median 43.3, range: 22.1 -94.7) and OSCC with a statistical significance of p<0.05. Conclusions: Our study provided evidence of increased salivary Her2/neu in OSCC when compared to PMD and control which was not the case for serum levels. This suggests that probably Her2/neu is not highly amplified as in breast cancer so as to be reflected in serum. Since saliva is in local vicinity of the OSCC, even a mild increase might be mirrored. On the whole, this study proposes Her2/neu as marker for distinguishing premalignant and malignant conditions.

• 한국식품영양과학회지
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• 제31권6호
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• pp.1078-1083
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• 2002

키토산을 응고제로 사용하여 상업 적 조건에서 제조한 두부와 일반 두부 및 키토산 분말을 고지질 급여 흰쥐 에6주간 급여한 후 혈청 지질대사에 미치는 영향을 조사하였다. 그 결과, 흰쥐의 식이 섭취량은 정상 식이군(N)에 비해 모두낮았으며, 키토산 두부 첨가군(CSC)과 키토산 분말 첨가군(CP)의 식이섭취량이 특히 낮았다. 체중 증가량은 고지질 식이군(HF)에 비해 CSC군, 일반 두부 첨가군(SC) 그리고 CP군들은 모두 유의적으로 낮았으며, 특히 CP군이 가장 낮은 체중증가를 나타내었다. 혈청중의 총 콜레스테롤 농도는 전 실험군간에 유의적인 차이는 없었으나, HDL-콜레스테롤 함량에서는 CSC, SC, CP군 모두 HF군에 비하여 높게 나타났다 따라서 총 콜레스테롤중 HDL-콜레스테롤이 차지하는 비율은 CSC, SC, CP군이 HF군에 비하여 높고 동맥경화지수는 반대로 낮게 나타났다. LDL-콜레스테롤 농도는 HF군에 비해 CSC, SC, CP 실험 군에서 유의적으로 낮게 나타났다. 총 지질 함량에서는 HF군에 비하여 SC와 CP군에서 유의적으로 감소하였다. 고지질 식이에 의해 증가한 중성지질 및 감소한 인지질 함량은 CSC, SC, CP군에서 유의적으로 회복되었다. 본 실험 에서는 HF군에 비해 CP군 뿐 아니라 CSC, SC군에서 HDL-콜레스테롤 및 인지질 농도의 증가, 중성지질 및 LDL-콜레스테롤 농도의 감소가 관찰됨으로써 상업용 키토산 두부가 고지질 관련 성인병 예방 및 치료에 효과가 있을 것으로 사료되었다. 그러나 현 실험조건하에서는 일반두부와 키토산 두부간에 효능에서 유의적인 차이를 발견하긴 어려웠다.

• Asian-Australasian Journal of Animal Sciences
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• 제30권12호
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• pp.1784-1795
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• 2017

Objective: The study examined the effect of intravenous administration of bacterial endotoxin-lipopolysaccharide (LPS) -on the nocturnal secretion of melatonin and on the expression of enzymes of the melatonin biosynthetic pathway in the pineal gland of ewes, taking into account two different photoperiodic conditions: short-night (SN; n = 12) and long-night (LN; n = 12). Methods: In both experiments, animals (n = 12) were randomly divided into two groups: control (n = 6) and LPS-treated (n = 6) one. Two hours after sunset, animals received an injection of LPS or saline. Blood samples were collected starting one hour after sunset and continuing for 3 hours after the treatment. The ewes were euthanized 3 hours after LPS/saline treatment. The concentration of hormones in plasma was assayed by radioimmunoassay. In the pineal gland, the content of serotonin and its metabolite was determined by HPLC; whereas the expression of examined genes and protein was assayed using real-time polymerase chain reaction and Western Blot, respectively. Results: Endotoxin administration lowered (p<0.05) levels of circulating melatonin in animals from LN photoperiod only during the first hour after treatment, while in ewes from SN photoperiod only in the third hour after the injection. Inflammation more substantially suppressed biosynthesis of melatonin in ewes from SN photoperiod, which were also characterised by lower (p<0.05) cortisol concentrations after LPS treatment compared with animals from LN photoperiod. In the pineal gland of ewes subjected to SN photoperiod, LPS reduced (p<0.05) serotonin content and the expression of melatonin biosynthetic pathway enzymes, such as tryptophan hydroxylase and arylalkylamine-N-acetyltransferase. Pineal activity may be disturbed by circulating LPS and proinflammatory cytokines because the expression of mRNAs encoding their corresponding receptors was determined in this gland. Conclusion: The present study showed that peripheral inflammation reduces the secretion of melatonin, but this effect may be influenced by the photoperiod.

• Asian Pacific Journal of Cancer Prevention
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• 제15권14호
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• pp.5815-5818
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• 2014

For an exact comparison of mRNA transcription in different samples or tissues with real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR), it is crucial to select a suitable internal reference gene. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and beta-actin (ACTB) have been frequently considered as house-keeping genes to normalize for changes in specific gene expression. However, it has been reported that these genes are unsuitable references in some cases, because their transcription is significantly variable under particular experimental conditions and among tissues. The present study was aimed to investigate which reference genes are most suitable for the study of gastric cancer tissues using qRT-PCR. 50 pairs of gastric cancer and corresponding peritumoral tissues were obtained from patients with gastric cancer. Absolute qRT-PCR was employed to detect the expression of GAPDH, ACTB, RPII and 18sRNA in the gastric cancer samples. Comparing gastric cancer with corresponding peritumoral tissues, GAPDH, ACTB and RPII were obviously upregulated 6.49, 5.0 and 3.68 fold, respectively. Yet 18sRNA had no obvious expression change in gastric cancer tissues and the corresponding peritumoral tissues. The expression of GAPDH, ${\beta}$-actin, RPII and 18sRNA showed no obvious changes in normal gastric epithelial cells compared with gastric cancer cell lines. The carcinoembryonic antigen (CEA), a widely used clinical tumor marker, was used as a validation gene. Only when 18sRNA was used as the normalizing gene was CEA obviously elevated in gastric cancer tissues compared with peritumoral tissues. Our data show that 18sRNA is stably expressed in gastric cancer samples and corresponding peritumoral tissues. These observations confirm that there is no universal reference gene and underline the importance of specific optimization of potential reference genes for any experimental condition.