• 한국식품영양학회지
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• 제8권4호
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• pp.275-279
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• 1995

A bacterium L-10 which produce mush of glutamic acid was Isolated from soil and identified as the genus Pserdomonas. The maximal glutamic acid production was obtained when the strain was cultured at 3$0^{\circ}C$ for 30 hrs in the optimal medium containing 5% glucose, 0.5% each of urea and yeast extract, 0.1% K2HP04, 0.02% MgSO4.7H20, 0.3% (NH, )rHP04, 0.5ug/l biotin and Initial pH 7.0, and then final glutamic acid production under the above conditions was 1.2mg/ml of cell cultures.

• Animal Bioscience
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• 제36권11호
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• pp.1685-1692
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• 2023

Objective: This study was conducted to investigate the effects of rumen-protected biotin (RPB) on growth performance, nutrient digestibility, nitrogen utilization and plasma biochemical parameters of Liaoning cashmere goats during the cashmere fiber growing period. Methods: Sixteen 6-month-old Liaoning cashmere twin-doelings (24.8±1.20 kg) were allocated to 2 diet groups that were individually ad libitum fed 30% concentrate and 70% forage diet (dry matter [DM]) by a paired experimental design. Goats of the control group were fed the basal diet, while goats belonging to the RPB group were fed the basal diet with 10 mg RPB/d per animal. The duration of the experiment was 16 weeks with two 8-week periods. Digestibility was determined at weeks 7 and 15, and other measures were taken every four weeks. Results: Compared with the control group, the average daily gain of the RPB group increased by 10.94% (p<0.05), and the intake of neutral detergent fiber was increased (p = 0.045). There were some increasing tendencies for the intake of DM, acid detergent fiber and ether extract (p = 0.070, 0.088, and 0.070, respectively). The intake and digestibility of N tended to increase (p = 0.062 and 0.093, respectively), while the N fecal excretion percentage of N intake was decreased (p = 0.093) in the RPB compared with the control group. N retention tended to increase (p = 0.084) with the addition of adding RPB to the diet. Plasma total protein was increased (p = 0.037), whereas the urea-N concentration was decreased (p = 0.049) in the RPB diet group compared with the control diet group. The levels of propionyl-CoA carboxylase (p<0.001) and methylmalonyl-CoA (p = 0.013) were increased in the RPB group. Conclusion: Supplementation of rumen-protected biotin in the diet of cashmere goats can enhance the utilization of N and improve daily weight gain during cashmere fiber growing period.

• 미생물학회지
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• 제12권3호
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• pp.115-130
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• 1974

Searches for the nutrition requirements of three strains of Brevibacterium ammoniagenes reported in the previous paper were carried out with an aim of achieving the striking accumulation of L-glutamic acid and the large multipication of cells. It was recognized that all three strains required both biotin and thiamine, together with amino acids such as histidine or cysteine, for their good growth and extracellular L-glutamic acid accumulation. The quantity of biotin required for remarkable growth of these microorganisms was quite different from that for the maximum production of L-glutamic acid. This result, however, did not apply in the case of thiamine. It was also confirmed that, of 18 amino acids, histidine and cysteine were the msot effective organic nitrogen sources, while the most available inorganic ammonium salt resulting in a large amount of L-glutamic acid-production and considerable cell gorwth was found to be only urea. Maximum accumulation of extracellular L-glutamic acid, more than 50%(w/w) of the initial sugar content, could be obtained from fermentation in the medium containing wheat-bran extract(Brev. ammoniagenes T-1 and Brev.ammoniagenes Y-2) or rice-bran extract(Brev. ammoniagenes YR-2), which confirmed us a possibility that these bacteria might be employed for industrial fermentation of L-glutamic acid.

• 한국식품과학회지
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• 제4권2호
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• pp.123-133
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• 1972

전보에서 분리 확보한 lysine 생산균주 Corynebacterium sp. S-27-12와 Brevibacterium flavum ATCC 15168 및 Micrococcus glutamicus ATCC 13032를 친주로 하여 자외선, $Co^{60}$및 N-methyl-N'-nitro-N-nitrosoguanidine treatment를 처리, 90여주의 변이주를 분리하고 이들의 lysine 생산능을 비교하여 lysine 생산이 8mg/ml 이상인 균주 6주를 선정하였다. 이들 중 leucine 요구주 Brev. flavum U46-N59의 lysine 축적조건을 검토한 결과를 종합하여 포도당 100, urea 10, CSL 40, $KH_2PO_4\;2;\;K_2HPO_4,\;0.5;\;MgSO_4.\;7H_2O,\;0.4;\;antifoam\;S-57,\;1g;\;Fe_2(SO_4)_3.XH-2O,\;10;\;MnCl_2,\;4H_2O,\;10mg;\;biotin,\;30;\;thiamine-HCl,\;100{\mu}g;$ 증류수 1 l로 된 pH7.5의 합성배지에 Brev. flavum U46-N59를 $28^{\circ}C$에서 4일간 플라스크(20ml/500ml)에 진탕배양했을때(180진탕/분) 최대로 21.6mg/ml의 lysine을 생산하였다. Penicilin은 40U/ml의 농도가 되도록 배양 36시간 후에 첨가하였다.

• 한국식품과학회지
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• 제9권1호
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• pp.1-9
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• 1977

A bacterium strain (K-173-10) which was isolated from waste soil of Korea brewing factory, could be grown on acetate as the sole carbon source and accumulated a considerable amount of L-glutamic acid in the medium. This strain was identified as the new species Brevibacterium ammoniagenes. This study was concerned not only with the culture condition for the production of L-glutamic acid and the cell growth, but also with the effects on concentration of various kind of organic substances, growth factors and penicillin. The results obtained were summarized as follow; 1. It was found that the concentrations of acetate and ammonium ions affected the growth of the bacterium as well as its L-glutamate accumulation. The optimum conditions of the composition of grown media for the growth of the bacterium and its glutamic acid production was found to be 40 g/l of total acetate, $100\;{\mu}g/l$ thiamine, $0.5\;{\mu}g/l$ biotin and $1{\sim}2g/l$ corn steep liquor as the growth factors. 2. Organic acid such as succinic acid, malic acid and ${\alpha}-ketoglutaric$ acid inhibited the cell growth as well as its L-glutamic acid production. 3. The penicillin (20 units/ml) stimulated the production of glutamic acid at appropriate incubation period. 4. It was found that this strain could grow in the presence of urea and ammonium acetate but not in other nitrogen sources.