• The Korean Journal of Physiology and Pharmacology
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• v.19 no.5
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• pp.451-460
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• 2015

Sirtuin 1 (SIRT1) is a mammalian $NAD^+$-dependent protein deacetylase that regulates cellular metabolism and inflammatory response. The organ-specific deletion of SIRT1 induces local inflammation and insulin resistance in dietary and genetic obesity. Macrophage-mediated inflammation contributes to insulin resistance and metabolic syndrome, however, the macrophage-specific SIRT1 function in the context of obesity is largely unknown. C57/BL6 wild type (WT) or myeloid-specific SIRT1 knockout (KO) mice were fed a high-fat diet (HFD) or normal diet (ND) for 12 weeks. Metabolic parameters and markers of hepatic steatosis and inflammation in liver were compared in WT and KO mice. SIRT1 deletion enhanced HFD-induced changes on body and liver weight gain, and increased glucose and insulin resistance. In liver, SIRT1 deletion increased the acetylation, and enhanced HFD-induced nuclear translocation of nuclear factor kappa B (NF-${\kappa}B$), hepatic inflammation and macrophage infiltration. HFD-fed KO mice showed severe hepatic steatosis by activating lipogenic pathway through sterol regulatory element-binding protein 1 (SREBP-1), and hepatic fibrogenesis, as indicated by induction of connective tissue growth factor (CTGF), alpha-smooth muscle actin (${\alpha}$-SMA), and collagen secretion. Myeloid-specific deletion of SIRT1 stimulates obesity-induced inflammation and increases the risk of hepatic fibrosis. Targeted induction of macrophage SIRT1 may be a good therapy for alleviating inflammation-associated metabolic syndrome.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1951-1964
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• 2016

1,4-Dioxane-degrading bacterial consortia were enriched from forest soil (FS) and activated sludge (AS) using a defined medium containing 1,4-dioxane as the sole carbon source. These two enrichments cultures appeared to have inducible tetrahydrofuran/dioxane and propane degradation enzymes. According to qPCR results on the 16S rRNA and soluble di-iron monooxygenase genes, the relative abundances of 1,4-dioxane-degrading bacteria to total bacteria in FS and AS were 29.4% and 57.8%, respectively. For FS, the cell growth yields (Y), maximum specific degradation rate ($V_{max}$), and half-saturation concentration ($K_m$) were 0.58 mg-protein/mg-dioxane, $0.037mg-dioxane/mg-protein{\cdot}h$, and 93.9 mg/l, respectively. For AS, Y, $V_{max}$, and $K_m$ were 0.34 mg-protein/mg-dioxane, $0.078mg-dioxane/mg-protein{\cdot}h$, and 181.3 mg/l, respectively. These kinetics data of FS and AS were similar to previously reported values. Based on bacterial community analysis on 16S rRNA gene sequences of the two enrichment cultures, the FS consortium was identified to contain 38.3% of Mycobacterium and 10.6% of Afipia, similar to previously reported literature. Meanwhile, 49.5% of the AS consortium belonged to the candidate division TM7, which has never been reported to be involved in 1,4-dioxane biodegradation. However, recent studies suggested that TM7 bacteria were associated with degradation of non-biodegradable and hazardous materials. Therefore, our results showed that previously unknown 1,4-dioxane-degrading bacteria might play an important role in enriched AS. Although the metabolic capability and ecophysiological significance of the predominant TM7 bacteria in AS enrichment culture remain unclear, our data reveal hidden characteristics of the TM7 phylum and provide a perspective for studying this previously uncultured phylotype.

• Journal of the Korean Geophysical Society
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• v.2 no.2
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• pp.135-142
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• 1999

Interpretations of 3-component magnetic logging data obtained for a reinforced bar as a model of the line of the magnetic dipoles are conducted using a least squared inversion technique. The length of the bar is 1.12 m, sampling interval is 0.05 m, the distance between the bar and the borehole is 0.3 m, and the top of the bar is fixed at 0 m of depth. The bar is set to be approximately vertical. Magnetic anomalies smoothed with FFT are used as input data for the inversion. For the interpretation of magnetic logging data the depth to the top, the length, the magnetic moment per unit length, the direction of the magnetization (declination and inclination), and the bearing and plunge of the line of magnetic dipoles are left as unknown parameters. The comparison of the results obtained from the individual inversion of the horizontal component or the vertical component of the magnetic anomalies, and those from the simultaneous inversion of horizontal and vertical component of the magnetic anomalies shows that there exist some disagreements between each inversion result. The depth to the bottom of the bar, which is actually 1.12 m, is estimated as 1.18 m, and the inclination of the magnetization is estimated as -76°by simultaneous inversion. The negative value of the inclination indicates that the strength of the remnant magnetization is much greater than that of the induced magnetization, so that the direction of the resultant magnetization points to the top of the bar.

• Journal of Gastric Cancer
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• v.1 no.2
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• pp.92-99
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• 2001

Purpose: E-cadherin is an adhesion molecule essential for tight connection between cells, forming the cadherin/catenin complex. Truncated $\beta$-catenin disrupts the interaction between E-cadherin and $\alpha$-catenin, leading to the loss of intercellular adhesion. Met protein, the hepatocyte growth factor receptor, plays important roles in signal transduction. We investigated the relationships between the expressions of E-cadherin, $\beta$-catenin, and c-met protein and the clinicopathological and prognostic parameters in gastric adenocarcinomas. Materials and Methods: The patterns of E-cadherin, $\beta$-catenin, and c-met protein expression were studied using immunohistochemistry in formalin-fixed, paraffin-embedded archival tissues from 76 surgically resected gastric adenocarcinomas. Results: Increased expressions of E-cadherin, $\beta$-catenin, and c-met were more significantly correlated in early gastric cancers (EGC) than in advanced gastric cancers (AGC) (P=0.002, P=0.003 and P=0.026). The positive immunoreactivities of all three markers were markedly lower in signet ring-cell type and poorly differentiated type lesions than in intestinal-type lesions. Decreased expression of the $\beta$-catenin protein correlated well with increased tumor invasion depth (P=0.039), and increased lymph node metastasis correlated well with reduced expression of c-met (P=0.046). Conclusion: In gastric cancers, reduced expressions of the E-cadherin, $\beta$-catenin, and c-met proteins may play some role in poorer tumor differentiation, deeper tumor invasion, and increased lymph node metastasis. Also, the c-met gene is thought to play a specific role in the mechanism of the yet unknown catenin action.

• Journal of Gastric Cancer
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• v.17 no.2
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• pp.132-144
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• 2017

Purpose: To identify baseline prognostic factors for survival in patients with disease progression, during or after chemotherapy for the treatment of advanced gastric or gastroesophageal junction (GEJ) cancer. Materials and Methods: We pooled data from patients randomized between 2009 and 2012 in 2 phase III, global double-blind studies of ramucirumab for the treatment of advanced gastric or GEJ adenocarcinoma following disease progression on first-line platinum- and/or fluoropyrimidine-containing therapy (REGARD and RAINBOW). Forty-one key baseline clinical and laboratory factors common in both studies were examined. Model building started with covariate screening using univariate Cox models (significance level=0.05). A stepwise multivariable Cox model identified the final prognostic factors (entry+exit significance level=0.01). Cox models were stratified by treatment and geographic region. The process was repeated to identify baseline prognostic quality of life (QoL) parameters. Results: Of 1,020 randomized patients, 953 (93%) patients without any missing covariates were included in the analysis. We identified 12 independent prognostic factors of poor survival: 1) peritoneal metastases; 2) Eastern Cooperative Oncology Group (ECOG) performance score 1; 3) the presence of a primary tumor; 4) time to progression since prior therapy <6 months; 5) poor/unknown tumor differentiation; abnormally low blood levels of 6) albumin, 7) sodium, and/or 8) lymphocytes; and abnormally high blood levels of 9) neutrophils, 10) aspartate aminotransferase (AST), 11) alkaline phosphatase (ALP), and/or 12) lactate dehydrogenase (LDH). Factors were used to devise a 4-tier prognostic index (median overall survival [OS] by risk [months]: high=3.4, moderate=6.4, medium=9.9, and low=14.5; Harrell's C-index=0.66; 95% confidence interval [CI], 0.64-0.68). Addition of QoL to the model identified patient-reported appetite loss as an independent prognostic factor. Conclusions: The identified prognostic factors and the reported prognostic index may help clinical decision-making, patient stratification, and planning of future clinical studies.

• Journal of the Society of Naval Architects of Korea
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• v.29 no.4
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• pp.234-243
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• 1992

Fatigue crack initiation life has a wide scatter and this makes the fatigue design of structural members difficult. In order to make the fatigue life distribution clear, it is required to prepare a large number of specimens and repeat the fatigue tests under the same loading condition. Such fatigue tests usually take much time and cost. In this study, a fatigue testing method using a multi-notched test specimen for the purpose of estimating the distribution function of fatigue crack initiation life by small number of fatigue tests is used. The purpose of this study is to verify the above fatigue testing method of a multi-notched specimen by using Bayesian reliability analysis, Least square method and Skewness method for the determination of unknown Weibull parameters. The multi-notched specimen is a specimen in which several tens of statistically identical notches are prepared.

• Journal of the Korea Computer Graphics Society
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• v.21 no.1
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• pp.1-11
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• 2015

Researchers have developed virtual reality environments to provide audience with more visually immersive experiences than previously possible. One of the most popular solutions to build the immersive VR space is a multi-projection technique. However, utilization of multiple projectors requires large spaces, expensive cost, and accurate geometry calibration among projectors. This paper presents a novel omnidirectional projection system with a single projector and a single spherical mirror.We newly designed the simple and intuitive calibration system to define the shape of environment and the relative position of mirror/projector. For successful image projection, our optimized omnidirectional image generation step solves image distortion produced by the spherical mirror and a calibration problem produced by unknown parameters such as the shape of environment and the relative position between the mirror and the projector. Additionally, the focus correction is performed to improve the quality of the projection. The experiment results show that our method can generate the optimized image given a normal panoramic image for omnidirectional projection in a rectangular space.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.40 no.12
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• pp.1032-1039
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• 2012

The present paper deals with precise relative positioning of formation satellites with long baseline in low Earth orbit making use of L1/L2 dual frequency GPS carrier phase measurements. Kinematic approach means to describe the motion of objects without taking its mass/dynamics model into consideration. The advantage of the kinematic approach is that information about dynamics of the system is not applied, which gives more flexibility and could improve the scientific interest of the observations made by the mission. The ionosphere terms, which are not canceled by double differenced measurement equation in the case of the long baseline, are explicitly estimated as unknown parameters by extended Kalman filter. The estimated float ambiguities by EKF are solved by existing efficient integer vector search strategy under integer least square condition. For the integer vector search, we employ well known MLAMBDA. Finally, The feasibility and accuracy of processing scheme are demonstrated using the GPS measurements for two satellites in low Earth orbit separated by baselines of 100 km.

• Journal of Korean Society of Steel Construction
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• v.19 no.2
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• pp.233-245
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• 2007

Previous finite element studies have shown that AASHTO Standard load distribution factor (LDF) equations appear to be conservative for longer spans and larger girder spacing, but too permissible for short spans and girder spacing. AASHTO LRFD specification defines the distribution factor equation for girder spacing, span length, slab thickness, and longitudinal stiffness. However, this equation requires an iterative procedure to correctly determine the LDF value due to an initially unknown longitudinal stiffness parameter. This study presents a simplified LDF equation for interior and exterior girders of two-span continuous I-girder bridges that does not require an iterative design procedure. The finite element method was used to investigate the effect of girder spacing, span length, slab thickness, slab width, and spacing and size of bracing. The computer program, GTSTRUDL, was used to idealize the bridge superstructures as the eccentric beam model, the concrete slab by quadrilateral shell elements, steel girders by space frame members, and the composite action between these elements by rigid links. The distribution factors obtained from these analyses were compared with those from the AASHTO Standard and LRFD methods. It was observed through the parametric studies that girder spacing, span length, and slab thickness were the dominant parameters compared with others. The LRFD distribution factor for the interior girder was found to be conservative in most cases, whereas the factor for the exterior girder to be unconservative in longer spans. Furthermore, a regression analysis was performed to develop simplified LDF formulas. The formulas developed in this study produced LDF values that are always conservative to those from the finite element method and are generally smaller than the LDF values obtained from the AASHTO LRFD specification. The proposed simplified equation will assist bridge engineers in predicting the actual LDF in two-span continuous I-girder bridges.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.77-81
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• 2007

Diagnostic performance of polymerase chain reaction (PCR) for detecting Dirofilaria immitis in dogs was evaluated when no gold standard test was employed. An enzyme-linked immunosorbent assay test kit (SnapTM, IDEXX, USA) with unknown parameters was also employed. The sensitivity and specificity of the PCR from two-population model were estimated by using both maximum likelihood using expectation-maximization (EM) algorithm and Bayesian method, assuming conditional independence between the two tests. A total of 266 samples, 133 samples in each trial, were randomly retrieved from the heartworm database records during the year 2002-2004 in a university animal hospital. These data originated from the test results of military dogs which were brought for routine medical check-up or testing for heartworm infection. When combined 2 trials, sensitivity and specificity of the PCR was 96.4-96.7% and 97.6-98.8% in EM and 94.4-94.8% and 97.1-98% in Bayesian. There were no statistical differences between estimates. This finding indicates that the PCR assay could be useful screening tool for detecting heartworm antigen in dogs. This study was provided further evidences that Bayesian approach is an alternative approach to draw better inference about the performance of a new diagnostic test in case when either gold test is not available.