• Animal Bioscience
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• 제35권10호
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• pp.1616-1627
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• 2022

Objective: This work was conducted to investigate the effects of oxidative stress on meat quality, mitochondrial function, calcium metabolism and ferroptosis of broilers. Methods: In this study, a total of 144 one-day-old male Ross 308 chicks were divided into 3 groups (control group, saline group, and hydrogen peroxide [H₂O₂] group) with 6 replicates of 8 broilers each. The study lasted for 42 d. The broilers in the saline and H₂O₂ groups were intraperitoneally injected with 0.75% saline and 10.0% H₂O₂ on the 16th and 37th day of the experimental period respectively, the injection volumes were 1.0 mL/kg of broiler body weight. On the 42nd day of the experimental period, two chicks were randomly selected from each cage, a total of thirty-six chicks were stunned by electric shock and slaughtered to collect breast muscle samples. Results: The H₂O₂ exposure reduced pH value, increased drip loss and shear force of breast meat (p<0.05), impaired the ultrastructure and function of mitochondria. The H₂O₂ exposure damaged the antioxidant system in mitochondria, excessive reactive oxygen species carbonylation modified calcium channels on mitochondria, which impaired the activities of key enzymes on calcium channel, resulted in the increased calcium concentration in cytoplasm and mitochondria (p<0.05). In addition, the H₂O₂ exposure increased the iron content and lipid peroxidation (p<0.05), which induced ferroptosis. Conclusion: Oxidative stress could impair meat quality by causing mitochondrial dysfunction, resulting in calcium metabolism disorder and ferroptosis.

• The Plant Pathology Journal
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• 제36권4호
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• pp.384-384
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• 2020

• 한국가스학회지
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• 제19권6호
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• pp.92-98
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• 2015

독성가스는 고압가스안전관리법에 의해 관리된다. 독성가스는 "고압가스안전관리법에서 규정한, 31종의 가스와 $LC_{50}$이 5000 ppm 이하인 가스"이다. 전자산업의 발달에 따라 우리나라 독성가스 사용량은 폭발적으로 증가하였다. 이에 국내 대학교 실험실의 독성가스 관련 연구 수요도 함께 증가해 왔다. 그러나 국내 실험실 독성가스 안전관리와 관련한 연구는 전무한 상태이다. 본 연구에서는 국내 대학 실험실의 실태조사를 통해 독성가스 안전관리에 있어서의 약점들을 파악하였다. 이 같은 약점을 극복하기 위해 독성가스 안전관리 방안을 제시하였다. 또한 제시된 방안에 따라 독성가스 시설이 안전하게 관리되는지를 확인하고, 이를 통한 개선을 위해 표준 체크리스트를 개발하였다. 이 연구 결과는 국내 대학 실험실에서 독성가스 시설의 안전관리를 강화할 것이며, 이를 모든 실험실에 대한 안전 지침으로 제공한다.

• Nuclear Engineering and Technology
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• 제51권5호
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• pp.1436-1443
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• 2019

A fast gamma-ray dose rate assessment method for complex geometries based on stylized model reconstruction and point-kernel method is proposed in this paper. The complex three-dimensional (3D) geometries are imported as a 3DS format file from 3dsMax software with material and radiometric attributes. Based on 3D stylized model reconstruction of solid mesh, the 3D-geometrical solids are automatically converted into stylized models. In point-kernel calculation, the stylized source models are divided into point kernels and the mean free paths (mfp) are calculated by the intersections between shield stylized models and tracing ray. Compared with MCNP, the proposed method can implement complex 3D geometries visually, and the dose rate calculation is accurate and fast.

• Nuclear Engineering and Technology
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• 제53권1호
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• pp.103-110
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• 2021

In the development of a Risk Monitor probabilistic safety assessment (PSA) model from the basic PSA model of a nuclear power plant, the modeling of common-cause failure (CCF) is very important. At present, some approximate modeling methods are widely used, but there lacks criterion of modeling accuracy and error analysis. In this paper, aiming at ensuring the accuracy of risk assessment and minimizing the Risk Monitor PSA models size, we present three basic issues of CCF model resulted from the changes of a nuclear power plant configuration, put forward corresponding modeling methods, and derive accuracy criteria of CCF modeling based on minimum cut sets and risk indicators according to the requirements of risk monitoring. Finally, a nuclear power plant Risk Monitor PSA model is taken as an example to demonstrate the effectiveness of the proposed modeling method and accuracy criteria, and the application scope of the idea of this paper is also discussed.

• Nuclear Engineering and Technology
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• 제54권1호
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• pp.293-300
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• 2022

During the decommissioning of the nuclear facilities, the radioactive gases in pressure vessels may leak due to the demolition operations. The decommissioning site has large space, slow air circulation, and many large nuclear facilities, which increase the difficulty of workers' inhalation exposure assessment. In order to dynamically evaluate the activity distribution of radionuclides and the committed effective dose from inhalation in nuclear decommissioning environment, an inhalation exposure assessment method based on the modified eddy-diffusion model and the inhaled dose conversion factor is proposed in this paper. The method takes into account the influence of building, facilities, exhaust ducts, etc. on the distribution of radioactive gases, and can evaluate the influence of radioactive gases diffusion on workers during the decommissioning of nuclear facilities.

• Structural Engineering and Mechanics
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• 제63권5호
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• pp.639-646
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• 2017

According to the multi-index system of dam safety assessment and the standard of safety, a comprehensive evaluation model for dam safety based on a cloud model is established to determine the basic probability assignment of the Dempster-Shafer theory. The Dempster-Shafer theory is improved to solve the high conflict problems via fusion calculation. Compared with the traditional Dempster-Shafer theory, the application is more extensive and the result is more reasonable. The uncertainty model of dam safety multi-index comprehensive evaluation is applied according to the two theories above. The rationality and feasibility of the model are verified through application to the safety evaluation of a practical arch dam.

• Asian-Australasian Journal of Animal Sciences
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• 제30권2호
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• pp.236-245
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• 2017

Objective: The study was to investigate the effects of alanyl-glutamine (Ala-Gln) and glutamine (Gln) supplementation on the intestinal mucosa barrier in piglets. Methods: A total of 180 barrows with initial weight $10.01{\pm}0.03kg$ were randomly allocated to three treatments, and each treatment consisted of three pens and twenty pigs per pen. The piglets of three groups were fed with control diet [0.62% alanine (Ala)], Ala-Gln diet (0.5% Ala-Gln), Gln diet (0.34% Gln and 0.21% Ala), respectively. Results: The results showed that in comparison with control diet, dietary Ala-Gln supplementation increased the height of villi in duodenum and jejunum (p<0.05), Gln supplementation increased the villi height of jejunum (p<0.05), Ala-Gln supplementation up-regulated the mRNA expressions of epidermal growth factor receptor and insulin-like growth factor 1 receptor in jejunal mucosa (p<0.05), raised the mRNA expressions of Claudin-1, Occludin, zonula occludens protein-1 (ZO-1) and the protein levels of Occludin, ZO-1 in jejunal mucosa (p<0.05), Ala-Gln supplementation enlarged the number of goblet cells in duodenal and ileal epithelium (p<0.05), Gln increased the number of goblet cells in duodenal epithelium (p<0.05) and Ala-Gln supplementation improved the concentrations of secretory immunoglobulin A and immunoglobulin G in the jejunal mucosa (p<0.05). Conclusion: These results demonstrated that dietary Ala-Gln supplementation could maintain the integrity of small intestine and promote the functions of intestinal mucosa barriers in piglets.

• 한국항행학회논문지
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• 제28권1호
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• pp.159-162
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• 2024

This paper proposes a novel safety autonomous platform (SAP) architecture that can automatically and precisely manage on-site safety through ensemble artificial intelligence models generated from video information, worker's biometric information, and the safety rule to estimate the risk index. We practically designed the proposed SAP architecture by the Hadoop ecosystem with Kafka/NiFi, Spark/Hive, Hue, ELK (Elasticsearch, Logstash, Kibana), Ansible, etc., and confirmed that it worked well with safety mobility gateways for providing various safety applications.

• Journal of Microbiology and Biotechnology
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• 제29권1호
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• pp.160-170
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• 2019

The lactic acid bacteria species Lactobacillus plantarum (L. plantarum) has been used extensively for vaccine delivery. Considering to the critical role of dendritic cells in stimulating host immune response, in this study, we constructed a novel CD11c-targeting L. plantarum strain with surface-displayed variable fragments of anti-CD11c, single-chain antibody (scFv-CD11c). The newly designed L. plantarum strain, named 409-aCD11c, could adhere and invade more efficiently to bone marrow-derived DCs (BMDCs) in vitro due to the specific interaction between scFv-CD11c and CD11c located on the surface of BMDCs. After incubation with BMDCs, the 409-aCD11c strain harboring a eukaryotic vector pValac-GFP could lead to more efficient expression of GFP compared with wild-type strains shown by flow cytometry analysis, indicating the enhanced translocation of pValac-GFP from L. plantarum to BMDCs. Similar results were also observed in an in vivo study, which showed that oral administration resulted in efficient expression of GFP in both Peyer's patches (PP) and mesenteric lymph nodes (MLNs) within 7 days after the last administration. In addition, the CD11c-targeting strain significantly promoted the differentiation and maturation of DCs, the differentiation of $IL-4^+$ and $IL-17A^+$ T helper (Th) cells in MLNs, as well as production of $B220^+$ $IgA^+$ B cells in the PP. In conclusion, this study developed a novel DC-targeting L. plantarum strain which could increase the ability to deliver eukaryotic expression plasmid to host cells, indicating a promising approach for vaccine study.