• The Journal of the Korean Society for Microbiology
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• v.3 no.1
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• pp.15-23
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• 1968

For the effective control of Syphilis, many investigators have developed a more rapid, simple and economical screening serological test which is adequately sensitive and specific. To fulfill the requirements of a more rapid serologic test for syphlis, a substitute for the conventional serum specimen was needed since considerable time and labor are involved in the processing of blood to serum. Burdon suggested the use of plasma in the serologic tests for syphilis as a substitute for serum. He noticed that plasma was more sensitive than serum in the Kline and Kahn tests, and attributed this to the presence of more antibody-like substance, "reagin" in plasma than in serum. However, to make plasma sufficiently sensitive, it was necessary to inactivate plasma by heating at a temperature of $56^{\circ}C$ for about 30 minutes. Heating of plasma resulted in the precipitation of fibrinogen which made centrifugation necessary to obtain dear plasma. Since the chief disadvantage to the use of unheated plasma(or serum) was a reduction in sensitivity of results-which probably was due to a labile factor such as complement-Portnoy et al began to consider rapid chemical methods of inactivation of plasma(or serum). They experienced that choline chloirde was shown to be anticomplementary which suggested its use as an inactivating agent for unheated plasma(or serum). In 1959 Portnoy et al reported the Rapid Plasma Reagin(RPR) Test for syphilis which is a more rapid, economical and simple. But still this test has many disadvantages as a rapid performing, field and office procedure, because it requires the usual laboratory equipments such as centrifuge, rotating machine, microscope etc. To substitute these disadvantages of the RPR test, in 1962, Portnoy et al developed the Rapid Plasma Reagin(RPR) card test for syphilis, which has the following advantages: a) Simplicity and rapidity of performance, b) Requires no laboratory equipments, c) Stable antigen suspension, d) Adequate sensitivity and specificity. This RPR card test can be used as a rapidly performing and screening test in field investigation, outpatient clinics, small laboratories and hospitals doing limited syphilis serology, and predonor in blood bank. Private clinic which has limited laboratory equipment and technic for syphilis serology can also use this RPR card test as a tool in the rapid diagnosis of syphilis. It was thought that this RPR card test is a useful tool in Korea for private physician and mass survey for syphilis diagnosis. But Portnoy patented the reagents needed for the performing the RPR card test. Therefore authors developed newly the reagents and according to Portnoy's method evaluated the newly developed. RPR card test compared with the VDRL, Kolmer CF, and RPCF tests. The RPR card and VDRL tests were performed plasma and serum from the total 1,132 cases. Among these 1,131 cases, 521 were syphilis suspected laboratory specimens, and 611 were syphilis unsuspected healthy young men. After screening with these two tests, the RPR card and VDRL tests, reactive specimens to the above one or both tests were retested by the Kolmer CF and RPCF tests.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.2
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• pp.155-161
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• 2002

Tetraselmis suecica (T. suecica) of Prasinophyta was selected because the growth rate is comparatively higher and the culturing is also easy. In order to investigate antioxidative activity, the soluble elements of T. suecica were fractionated using water and organic solvents such as methanol, hexane, chloroform, ethylacetate and butanol. The chloroform fraction of T. suecica showed strong antioxidative activity. The potential antioxidative activity was detected in hexane: ethylacetate (1:5) once used the fractions by different mixtures of organic solvents. This fraction was further purified by preparative thin layer chromatography (PTLC) and repeated reverse-phase HPLC. On the basis of chemical and spectroscopic evidences obtained by UV, FT-IR, FAB-MS and NMR, the compound purified from T. suecica was identified as pheophorbide-a. The antioxidative activity of the compound was comparable to that of $\alpha$-tocopherol and could be act as an antioxidant in foods.

• BMB Reports
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• v.28 no.6
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• pp.527-532
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• 1995

Human taurine transporter has 12 transmembrane domains and its molecular weight is 69.6 kDa. The long cytoplasmic carboxy and amino termini might function as regulatory attachment sites for other proteins. Six potential protein kinase C phosphorylation sites have been reported in human taurine transporter. In this report, we studied the effects of phorbol 12-myristate 13-acetate (PMA) and glucocorticoid hormone on taurine transportation in the RAW 264.7, mouse macrophage cell line. When the cells were incubated with $[^{3}H]taurine$ in the presence or absence of $Na^+$ ion for 40 min at $37^{\circ}C$, the [$[^{3}H]taurine$ uptake rate was 780-times higher in the $Na^{+}-containing$ buffer than in the $Na^{+}-deficient$ buffer, indicating that this cell line expresses taurine transporter protein on the cell surface. THP1, a human promonocyte cell line, also showed a similar property. The $[^{3}H]taurine$ uptake rate was not influenced by the inflammatory inducing cytokines such as interleukin-1, gamma-interferon or interleukin-1+gamma-interferon, but was decreased by the PMA in the RAW 264.7 cell line. This suggests that activation of protein kinase C inhibits taurine transporter activity directly or indirectly. The inhibition of $[^{3}H]taurine$ uptake by PMA was time-dependent. Maximal inhibition occurred in one hr stimulation with PMA Increasing the treatment time beyond one h reduced the $[^{3}H]taurine$ uptake inhibition due to the depletion or inactivation of protein kinase C. The cell line also showed concentration-dependent $[^{3}H]taurine$ uptake under PMA stimulation. The phorbol-ester caused 23% inhibition at the concentration of 1 ${\mu}m$ PMA. The inhibition was significant even at a concentration as low as 10 nM PMA The reduced $[^{3}H]taurine$ uptake could be recovered by treatment with glucocorticosteroid hormone. Dexamethasone led to recover of the reduced taurine uptake induced by phorbol-ester, recovering maximally after one hr. This may suggest that macrophage cells require higher taurine concentration in a stressed state, for the secretion of glucocorticoid hormone is increased by hypothalamo-pituitary-adrenocortical (HPA) axis activation in the blood stream.

• Korean Journal of Optics and Photonics
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• v.19 no.3
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• pp.182-186
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• 2008

The prism spectrometer is a standard device for the measurement of refractive index; it is used in undergraduate laboratories. Typically, however, lots of attention is required in the alignment, and the accuracy of the obtained refractive index is not so high in spite of the durability of the device. The maximum and minimum deviation method, which compensates the disadvantages of the prism spectrometer, can be composed cost effectively using a length marking tape and a rotating platform. It can measure the refractive indices accurately by utilizing a wide screen. In this study, the equal sided hollow prism whose length is 26 mm was fabricated and measured the refractive indices of seven kind of liquids (pure water, $C_3H_5(OH)_2$, $CCl_4$, $C_6H_4NH_2$, $CS_2$, $C_6H_4(CH_3)_2)$ by using the prism spectrometer and maximum and minimum deviated laser beam method at the wavelengths of He-Ne laser (${\lambda}$= 632.8 nm) and YVO4 laser (${\lambda}$= 532 nm). The result shows that the data obtained by the latter method are more accurate and precise than those obtained by the former device.

• Korean Chemical Engineering Research
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• v.54 no.2
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• pp.145-151
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• 2016

In response to the cavitation caused by the partial vacuum caused by the fluid flow, a paint was developed by dispersing the lamella-shaped glass-flake in resin for anti-cavitation. This composite paint was developed by using the inorganic filler (lamella shaped glass-flake) and the NBR (Acrylonitrile-butadiene rubber) which was modified epoxy resin. Especially, the glass-flake was a thin film with a thickness of about 100~200 nm and length of about $20{\sim}30{\mu}m$, the aspect ratio was about 200 to 300 times that of the plate-shaped. So the paint for anti-cavitation have shown excellent performance in corrosion resistance. The results of evaluating anti-cavitation performance was below, tensile strength $4.8{\sim}6N/mm^2$ or more, rupture elongation 30% or higher, abrasive speed $10mm^2/h$ or less. In particular, it showed more than twice the superior performance compared to existing advanced foreign products in anti-cavitation performance evaluation.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1994.06a
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• pp.11-26
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• 1994

Crown gall of stonefruit and nut trees is one of the very few plant diseases subject to efficient biological control. The disease is caused by the soil-inhabiting bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes and the original control organism was a non-pathogenic isolate of A. rhizogenes strain K84. Control is achieved by dipping planting material in a cell suspension of strain K84 which specifically inhibits pathogenic strains containing a nopaline Ti plasmid. Because the agrocin 84-encoding plasmid (pAgK84) is conjugative, it can be transmitted from the control strain to pathogenic strains which, as a result, become immune to agrocin 84 and cannot be controlled. To prevent this happening, the transfer genes on pAgK84 were located and then largely eliminated by recombinant DNA technology. The resulting construct, strain K1026, is transfer deficient but controls crown gall just as effectively as does strain K84. Field data from Spain confirm that pAgK84 can transfer to pathogenic recipients from strain K84 but not from strain K1026. The latter has been registered in Australia as a pesticide and is the first genetically engineered organism in the world to be released fro commercial use. It is recommended as a replacement for strain K84 to prevent a breakdown in the effectiveness of biological control of crown gall. Several reports indicate that both strains K84 and K1026 sometimes control crown gall pathogens that are resistant to agrocin 84. A possible reason for this is that both strains produce a second antibiotic called 434 which inhibits growth of nearly all isolates of A. rhizogenes, both pathogens and non-pathogens. Crown gall of grapevine is caused by another species, Agrobacterium vitis. It is resistant to agrocin 84 and cannot be controlled by strains K84 or K1026. It is different from other crown gall pathogens in several characteristics, including the fact that, although a rhizosphere coloniser, its also lives systemically in the vascular tissue of grapevine. Pathogen free propagating material can be obtained from tissue culture or, less surely, by heat therapy of dormant cuttings. A number of laboratories are searching for a biocontrol strain that will prevent, or at least delay, reinfection. A non-pathogenic A. vitis strain F/25 from South Africa looks very promising in this regard.

• Journal of the Korea Institute of Building Construction
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• v.19 no.3
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• pp.209-217
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• 2019

EMP (Electromagnetic Pulse) usually means High Power Electromagnetic Wave (HPEM). In the case of the shielding plate against the EMP, there is a possibility of deterioration of the electromagnetic wave shielding performance due to the skill of the constructor, bad construction, deformation of the shielding plate at the connection portion (joint portion). The inefficient use of space due to the separation distance is also pointed out as a problem. Therefore, this study aims to derive the optimum electromagnetic shielding condition by applying ATMSM to concrete as a part of securing electromagnetic wave shielding performance with reflection loss against concrete wall. Experimental parameters included concrete wall thickness and application of Zn-Al ATMSM. For the concrete wall, the wall thickness was 100 to 300mm, which is generally applied, and experimental parameters were set for the application of Zn-Al metal spraying method to evaluate electromagnetic shielding performance. Experimental results showed that as the thickness increases, the electromagnetic shielding performance increases due to the increase of absorption loss. In addition, after the application of Zn-Al ATMSM, the average shielding performance increased by 56.68 dB on average, which is considered to be increased by the reflection loss of the ATMSM. In addition, it is considered that the shielding performance will be better than that when the conductive mixed material and the ATMSM are simultaneously applied.

• Membrane Journal
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• v.32 no.4
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• pp.253-263
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• 2022

This study suggests a guideline for designing unit process of wastewater reuse in terms of a maintenance of the process based on critical parameters to draw a high quality performance of RO unit. Defining the parameters was done by applying membrane integrity test (MIT) in pretreatment process utilizing lab-scale MF. SDI is utilized for judging whether permeate is suitable to RO unit. However, result said TOC concentration matching with particle count analysis is better for judging the permeate condition. When membrane test pressure (P_test) was measured to derive log removal value in PDT, virgin state of membrane fiber was used to measure dynamic contact angle utilizing surface tension of the membrane fiber. Actually, foulant affects to the state of membrane surface, and it decreases the P_test value along with time elapsed. Consequently, LRV_DIT is also affected by P_test value. Thus, sensitivity of direct integrity test descends with result of P_test value change, so P_test value should be considered not the virgin state of the membrane but its current state. Overall, this study focuses on defining design parameters suitable to MF pretreatment for RO process in wastewater reuse by assessing its impact. Therefore, utilities can acknowledge that the membrane surface condition must be considered when users conduct the direct integrity test so that P_test and other relative parameter used to calculate LRV_DIT are adequately measured.

• Journal of the Korean Recycled Construction Resources Institute
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• v.12 no.3
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• pp.254-262
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• 2024

The demand for high strength and ultra-lightweight materials to incorporate the advanced technology of nanomaterials into the lengthening of structures is continuously increasing. Therefore, based on existing research results and numerous mixing trials, we derived a mix of high strength and ultra-light concrete of a compressive strength of 100 MPa with a unit weight of 18 kN/m³ and a compr essive str ength of 80 MPa with a unit weight of 16 kN/m³ and evaluated their per for mance. In this paper, 108 specimens corresponding to high strength and ultra-lightweight concrete with a compressive strength of 100 MPa under a unit weight of 18 kN/m³, and a compressive strength of 80 MPa under a unit weight of 16 kN/m³ were manufactured, and the bond characteristics were identified by performing a directly tensile tests, and the bond characteristics were evaluated by comparing them with the experimental results and the current design criteria. It was judged that the bond strength calculation formula of ACI-408R and the experimental results were not accurately reflected, so an bond stress equation based on ACI-408R was proposed. The result of the proposed equation was that the deviation was somewhat reduced. In addition, the results of calculating the CEB-FIP model and the modified CMR model using statistical analysis showed slight differences from the experimental results, but considering that the bond behavior is a local behavior, the proposed model appears to explain the bond behavior of high strength and ultra-light concrete as a whole.

• Journal of the Korean Recycled Construction Resources Institute
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• v.6 no.2
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• pp.115-122
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• 2018

In this paper, the feasibility of recycling concrete waste as a method to reduce final disposal amount of wastes generated through decommissioning of nuclear power plant has been analyzed based on experimental results of existing literature. When recycled concrete waste was used as recycled aggregate, it was investigated through literature that the concrete strength decreased by 30~40% depending on the mixing ratio. It was also investigated that concrete with recycled aggregate can be used as a structural material when the quality of recycled aggregate is well managed since no significant problem was found. When recycled cement produced from concrete waste was used, the strength of concrete or mortar decreased considerably as the recycled cement content increased. Therefore, it can be concluded that concrete or mortar with recycled cement can be used as a filling material for final disposal of large radioactive waste rather than for structural use. This paper is expected to be useful for reduction on disposal volume and decommissioning cost for nuclear power plants such as Kori 1.