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Analysis of Genetic Relationship and Cultural Characteristics of Penicillium species Isolated from Postharvest Decay of Pear by Random Amplified Polymorphic DNA (저장 배에서 분리한 Penicillium속의 배양적 특성과 RAPD에 의한 유연관계 분석)

  • Kim, Ju-Hee;Lee, Wang-Hyu;Ryu, Young-Jin;Cheong, Seong-Soo;Choi, Joung-Sik
    • The Korean Journal of Mycology
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    • v.30 no.2
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    • pp.78-85
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    • 2002
  • Fifteen isolates from pear, and sixteen isolates from KCTC, KCCM, and Chungnam Univ. of Penicillium spp. were investigated for the analysis of their relationships of cultural characteristics and RAPD genetic variation by RAPD. The cultural characteristics of Penicillium spp. were shown different growth rate, morphology, and color. In addition, the cultural characteristics and RAPD analysis were conducted for the pear rot pathogens and related isolates. RAPD patterns were applied to compare the taxonomic and genetic diversity of the Penicillium species between 15 groups isolated from pear fruits and 16 standard species. The genomic DNA were amplified from $0.1{\sim}2.0kb$ by five URP primer and 744 bands were detected. The cluster analysis showed four genomic DNA RAPD groups and its similarity was 47.7%. Intraspecific relationships were 87.4, 97.5 and 95.2%, in P. expansum, P. solitum, and P. crustosum, respectively. These results appeared to be that there were high similarities between isolates, and consistent with the results of cultural morphological characteristics analysis.

Restoration planning of the Seoul Metropolitan area, Korea toward eco-city

  • Lee, Chang Seok
    • Proceedings of the Korea Society of Environmental Biology Conference
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    • 2003.06a
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    • pp.1-5
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    • 2003
  • In order to prepare a basis for ecological restoration of the Seoul Metropolitan area, ecological diagnoses on soil physico-chemical properties and vegetation structure were carried out. Land use patterns, actual vegetation, and biotope patterns were also investigated based on aerial photograph interpretation and field checks. I formulated landscape elements overlaying those data and evaluated the ecological value of each element. Soil pollution was evaluated by analyzing soil samples collected in each grid on the mesh map, divided by 2km $\times$ 2km intervals. Soil samples were collected in forests or grasslands escaped from direct human interference. Soil pollution evaluated from pH, and SO$_4$, Ca, Mg, and Al contents of soil was more severe in the urban outskirts than in the urban center. Those soil environmental factors showed significant correlation with each other. Vegetation in the urban area was different in species composition from that in suburban areas and showed lower diversity compared with that in the suburban areas. Successional process investigated by population structure of major species also showed a difference. That is, successional trend was normal in suburban areas, but that in urban areas showed a retrogressive pattern. The landscape ecological map of Seoul indicates that the urban center lacks vegetation and greenery space is restricted in urban outskirts. Such an uneven distribution of vegetation has caused a specific urban climate and thereby contributed to aggravation of air and soil pollution, furthermore causing vegetation decline. From this result, it was estimated that such uneven distribution of vegetation functioned as a trigger factor to deteriorate the urban environment. I suggested, therefore, a restoration plan based on landscape ecological principles, which emphasizes connectivity and even distribution of green areas throughout the whole area of the Seoul to solve this complex environmental problem. In this restoration plan, first of all, I decided the priority order for connection of the fragmented greenery spaces based on the distances from the core reserves comprised of green belt and rivers, which play roles as habitats of wildlife as well as for improvement of urban environment. Next, I prepared methods to restore each landscape element included in the paths of green network to be constructed in the future on the bases of such preferential order. Rivers and roads, which hold good connectivity, were chosen as elements to play important roles in constructing green network by linking the fragmented greenery spaces.

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The Comparison of Health Promoting Behaviors for the Korean Elderly residing in Korea and Japan (한국거주와 일본거주 한국노인의 건강증진 행위 비교)

  • Park, Kyung-Min
    • Research in Community and Public Health Nursing
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    • v.13 no.1
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    • pp.68-78
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    • 2002
  • This study intended to analyze the health promoting behaviors by comparing various factors according to the residence areas(Korea or Japan) of Korean Elderly. and to present basic data in planning systematic and effective programs of health promotion for each residence area. The subjects of this study were 164 Korean elders in Daegu, South Korea, and 164 elders in Aichi prefecture, Japan. Data was collected by interviews and self-administered questionnaires from the 1st to the 20th of April in 2000. The tool for this study was based on Walker et al.(1987)'s Health Promotion lifestyle Profiles (HPLP). The analysis was performed with Cronbach's $x^2-test$, t-test. ANCOVA, Kendal tau, Pearson correlation, and Stepwise Multiple Regression test using SPSS program. The results are as follows: 1. There was a significant difference in occupation($x^2$=41.3. p=0.000), the source of the pocket money($x^2$=114.36. p=0.000) by the residence areas in terms of socio-demographic characteristics. 2. There was a significant difference between the two groups on the health promoting behaviors(t=-8.19. p=0.000). The score of elders group in the South Korean group on health promoting behaviors was 2.32. showing 0.33 lower than that of the elders group in Japan(2.65). 3. ANCOVA involving occupation and the source of pocket money as co-variables. showed significant differences (F=15.37. p=0.000) regarding health promoting behaviors according to the residence areas. 4. In consideration of variables that have an influence on health promoting behavior by residence areas, pocket money occupied 11.5% of health promoting behavior in the elders group in South Korea. In the elders group in Japan, pocket money occupied 18.1% of health promoting behavior and 20.6% including education. The suggestions based on the results of this study are as follows.: 1. It is necessary to develop health promoting programs considering the residence areas of Korean elders. 2. It is necessary to develop social programs for improving the education level and solving pocket money problems, which are the significant factors for the health promoting behavior of Korean elders.

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A Study on the Tidal Current State of Myeongnyang Strait on the Date of Myeongnyang Sea Battle, by Orbital Period of Celestial Body (천체의 궤도 운동 주기 분석을 통한 명량대첩 당시의 명량수도 조류 상태에 관한 연구)

  • Kim, Hyun-Jong
    • Journal of Navigation and Port Research
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    • v.39 no.3
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    • pp.209-215
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    • 2015
  • Miraculous victory of Myeongnyang sea battle turned the tide of the Joseon's entire war against Japan and it is regarded as one of the most remarkable sea victories in the world history. In the sea battle of Myeongnyang, on September 16, 1597(lunar calender), the Joseon navy with 13 battle ships, led by Admiral Yi Sun-sin, won the Japanese navy with their fleet of 133 warships. There were several reasons why Admiral Yi decided on this location for battle. Myeongnyang strait is so narrow and had currents so powerful that many ships could not pass strait simultaneously. Therefore, despite being vastly outnumbered, Admiral Yi used terrain and tidal current advantage to defeat Japanese navy's numerical advantage. In order to find out the tidal state of Myeongnyang strait on September 16, 1597, topological phase of sun and moon was studied by orbital period of earth and moon. The tidal state of Myeongnyang strait on September 16, 1597 is estimated based on the theories of tide and tide tables. As a result of this study, time of slack water were found to be 0636, 1248, 1906 and time/speed of maximum tidal current were found to be 0930/8.3kts(NW), 1612/9.9kts(SE).

Influence of Ammonia and Dissolved Oxygen Concentrations on Nitrite Accumulation in a MBR (MBR 반응조에서 아질산염 축적에 미치는 암모니아와 용존산소 농도의 영향 연구)

  • Choi, In-Su;Wiesmann, Udo
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.8
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    • pp.922-929
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    • 2007
  • The complete oxidation of ammonia to nitrate is a distinctive two-step process divided into the oxidation of ammonia to nitrite(nitritation) by Nitrosomonas and the oxidation of nitrite to nitrate(nitratation) by Nitrobacter. The nitrogen removal via nitrite accumulation offers several advantages such as saving costs for aeration, saving carbon source and finally reduction of sludge discharge. In this work a suspended bioreactor coupled with membrane filtration(MBR) was used to find the process conditions of nitrite build-up. The MBR enables to reach sufficient nitrifying bacteria in the bioreactor, although the autotrophic bacteria can be easily washed out due to their lower growth rate. The dissolved oxygen concentration $c'_{O2}$ and ammonia concentration $c_{NH3}$ in the reactor were varied and investigated as parameters for nitrite accumulation. As a result the higher ammonia concentration in the reactor is very effective for starting nitrite build-up and the effect was strengthened in combination with lower dissolved oxygen concentration. With lower $c'_{O2}<0.3$ $mgL^{-1}$ $O_2$ and high $c_{NH3}=6.3\sim14.9$ $mgL^{-1}$ $NH_3N$ the 74% of the nitrite accumulation was achieved. Specially, it was found that the nitrite accumulation could occur not only in biofilm reactor as many references showed but also in the membrane bioreactor carried out in this study.

Studies on the Production of Artificial Zeolite from Coal Fly Ash and Its Utilization in Agro-Environment

  • Lee, Deog-Bae;Henmi, Teruo;Lee, Kyung-Bo;Kim, Jae-Duk
    • Korean Journal of Environmental Agriculture
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    • v.19 no.5
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    • pp.401-418
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    • 2000
  • 1. Production of the artificial zeolite from coal ash Coal fly ash is mainly composed of several oxides including $SiO_2$ and $Al_2O_3$ derived from inorganic compounds remained after burning. As minor components, $Fe_2O_3$ and oxides of Mg, Ca, P, Ti (trace) are also contained in the ash. These components are presented as glass form resulting from fusion in the process of the combustion of coal. In other word, coal ash may refer to a kind of aluminosilicate glass that is known to easily change to zeolite-like materials by hydrothermal reaction. Lots of hot seawater is disposing near thermal power plants after cooling turbine generator periodically. Using seawater in the hydrothermal reaction caused to produce low price artificial zeolite by reduction of sodium hydroxide consumption, heating energy and water cost. As coal ash were reacted hydrothermally, peaks of quartz and mullite in the ash were weakened and disappeared, and new Na-Pl peaks were appeared strengthily. Si-O-Si bonding of the bituminous coal ash was changed to Si-O-Al (and $Fe^{3+}$) bonding by the reaction. Therefore the produced Na-Pl type zeolite had high CEC of 276.7 $cmol^+{\cdot}kg^{-1}$ and well developed molecular sieve structure with low concentration of heavy metals. 2. Utilization of the artificial zeolite in agro-environment The artificial zeolite(1g) could remove 123.5 mg of zinc, 164.7 mg copper, 184.4 mg cadmium and 350.6 mg lead in the synthetic wastewater. The removability is higher 2.8 times in zinc, 3.3 times in copper, 4.7 times in cadmium and 4.8 times in lead than natural zeolite and charcoal powder. When the heavy metals were treated at the ratio of 150 $kg{\cdot}ha^{-1}$ to the rice plant, various growth inhibition were observed; brownish discoloration and death of leaf sheath, growth inhibition in culm length, number of panicles and grains, grain ripening and rice yield. But these growth inhibition was greatly alleviated by the application of artificial zeolite, therefore, rice yield increased $1.1{\sim}3.2$ times according to the metal kind. In addition, the concentration of heavy metals in the brown rice also lowered by $27{\sim}75%$. Artificial Granular Zeolites (AGZ) was developed for the purification of wastewater. Canon exchange capacity was 126.8 $cmol^+{\cdot}kg^{-1}$. AGZ had Na-Pl peaks mainly with some minor $C_3S$ peaks in X-ray diffractogram. In addition, AGZs had various pore structure that may be adhere the suspended solid and offer microbiological niche to decompose organic pollutants. AGZ could remove ammonium, orthophosphate and heavy metals simultaneously. Mixing ratio of artificial zeolite in AGZs was related positively with removal efficiency of $NH_4\;^+$ and negatively with that of $PO_4\;^{3-}$. Root growth of rice seedling was inhibited severely in the mine wastewater because of strong acidity and high concentration of heavy metals. As AGZ(1 kg) stayed in the wastewater(100L) for 4days, water quality turned into safely for agricultural usage and rice seedlings grew normally.

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Comparative Analyses of the Uses of Information and Information Technologies in the Everyday Life of Undergraduate and Graduate Students Majoring Science and Engineering (넷세대 이공계 학부생과 대학원생들의 학업 및 일상생활에서의 정보이용과 정보기기 활용 비교분석)

  • Kwon, Nahyun;Lee, Jungyeoun;Chung, Eunkyung;Chang, Gilsoo;Yoon, Hyejin
    • Journal of the Korean Society for information Management
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    • v.30 no.2
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    • pp.269-295
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    • 2013
  • This study purported to study the uses of information and communication technology (ICT) tools in their daily life among the undergraduate and graduate students majoring science and engineering. It also examined the purposes of the uses of the major ICT tools, the differences in their information technology uses between graduate and undergraduate students. The data were collected from 83 undergraduate and graduate students from four universities using content analysis of an observational logs and a survey questionnaire. The observational logs were collected using an instant message application available on a smart phone. Study results revealed desktop computers, laptop computers, and smart phones as the three most heavily used ICT tools among the participants and typical situations students use each of the three tools. There were also sharp distinctions between graduate and undergraduate students in their selection of the tools, and distinctions between work and non-work situations. The findings of this study can be used to redesign information services and systems for the scientists and engineers in the next generation.

Effects of Variety and Stand Density on Plant Morphology, Winter Survival, and Cold Hardiness of Alfalfa (품종과 재식밀도가 알팔파의 형태, 월동 및 내한성에 미치는 영향)

  • Min, D.H.;King, J.R.;Kim, D.A.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.2
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    • pp.167-176
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    • 1999
  • Shoot morphological characteristics were affected by plant population densities in alfalfa. Shoots per plant, yield per shoot, yield per plant, and stem diameter increased with decreasing alfalfa population density. Winter survival was maximized at a plant population of $45plants\;m^{-2}$ or less. The grams of TNC per root increased as the stand density decreased. The lowest LT 50 occurred at the lowest stand density ($16plants\;m^{-2}$ having 25 cm plant spacing) and the highest LT 50 was attained at the highest stand density($494plants\;m^{-2}$ having 4.5 cm plant spacing). There were no differences in shoot morphology, winter survival, and cold hardiness in between 'Algonquin'and 'Vernal'. From this study, high stand density of alfalfa ($278plants\;m^{-2}$ or more) appears to be of little advantage compared to low stand density in terms of winter survival and cold hardiness.

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Homo replicus: imitation, mirror neurons, and memes (호모 리플리쿠스(Homo replicus): 모방, 거울뉴런, 그리고 밈)

  • Jang, Dayk
    • Korean Journal of Cognitive Science
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    • v.23 no.4
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    • pp.517-551
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    • 2012
  • We are imitating animals. True imitation can be defined as a learning to do an act from seeing it done by others. We have been building culture by imitating others' skills and knowledge with high fidelity. In this regard, it is important to ask how the faculty of imitation has evolved and how imitation behaviors develop ontogenetically. It is also interesting to see whether nonhuman animals can imitate truly or not and how different imitation learning is among human and non-human animals. In this paper, first I review empirical data from imitation studies with human and nonhuman animals. Comparing different species, I highlight their different levels of copying fidelity and explain the reason why they are showing the difference. Then I review recent studies on neurobiological mechanisms underlying imitation. The initial neurobiological studies on imitation in humans suggested a core imitation circuitry composed of mirror neuron system [inferior parietal lobule(IPL) and inferior frontal gyrus(IFG)] and the posterior part of the superior temporal sulcus(pSTS). More recent studies on the neurobiology of imitation, however, has gone beyond the studies on the core mechanisms. Finally, I try to find out implications of psychology and biology of imitation for cultural evolution. I argue for a memetic approach to cultural evolution, along the lines with a recent study on measuring memes by mirror neurons system.

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Growth, Hay Yield and Chemical Composition of Cassava and Stylo 184 Grown under Intercropping

  • Kiyothong, K.;Wanapat, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.6
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    • pp.799-807
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    • 2004
  • The objective of this field experiment was to investigate the growth, hay yield and chemical composition of cassava and stylo 184 grown under intercropping. The experiment was arranged in a Randomized Complete Block (RCB) design with 5 treatments and 4 replications. The treatments were: sole crop cassava (C); sole crop stylo 184 (S) and three intercropping treatments comprising an additive series of one (SC), two (SSC) and three (SSSC) rows of stylo 184 to one row of cassava. The results showed that leaf area per plant (LA) of cassava was significantly higher (p<0.05) in the sole crop relative to the intercropping treatments. Both total hay yield and CPDM yield were significantly higher (p<0.05) for C treatment and lower (p<0.05) for SSSC treatment. The total hay yield and CPDM yield were significantly greater (p<0.05) in the sole crop relative to the intercropping treatments. At the first and second harvests, CP content was similar among treatments; while at third and fourth harvests, CP contents were significantly greater (p<0.05) for the intercropping treatment relative to the sole crop. At the first and second harvest, NDF contents were significantly greater (p<0.05) in the sole crop relative to the intercropping treatments, whereas NDF contents were similar among intercropping treatments. Leaf area of stylo 184 at first and second harvest were significantly greater (p<0.05) for C, SC and SSC as compared with the SSSC treatments. At each harvesting, there were no significant differences in ash, CP, NDF, ADF and ADL contents of stylo 184 hay between the sole crop and intercropping treatments, except for the first harvest. ADF contents were significantly greater (p<0.05) in S, SC treatments relative to SSC and SSSC treatments. Both collective hay yield and CPDM yield of cassava and stylo 184 were significantly greater (p<0.05) for the SSC treatment and significantly lower (p<0.05) for the S treatment. Collective hay yield and CPDM yield were significantly greater (p<0.05) for the intercropping treatments relative to the sole crop. Based on this research, it was concluded that stylo 184 showed potential for intercropping with cassava. Intercropping cassava with stylo 184 has beneficial effects and can improve foliage biomass yield and soil fertility, which would be a more sustainable system than growing the cassava as a pure stand. In terms of hay yield and CP production, two rows of stylo 184 to one row of cassava could be the optimal pattern for this intercropping system.