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Development of a SNP marker set related to crown gall disease in grapevines by a genome wide association study

  • Kim, Dae-Gyu;Jang, Hyun A;Lim, Dong Jun;Hur, Youn Young;Lee, Kyo-Sang;Min, Jiyoung;Oh, Sang-Keun
    • Korean Journal of Agricultural Science
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    • v.47 no.3
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    • pp.693-705
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    • 2020
  • Grapes (Vitis spp. L.) are the third most produced fruit in the world. Crown gall disease caused by Agrobacterium vitis forms galls in the stems of the grapevines and reduces the vitality of the fruit trees, resulting in reduced yields. This pathogen has occurred in vineyards worldwide and caused serious economic losses. It is a soil-borne disease, so Agrobacterium vitis can survive for several years in vineyards and is difficult to control. Additionally, since there is no effective chemical control method, the most effective control method is the breeding of resistant varieties. To make the resistant variety, marker-assisted selection (MAS) enables fast breeding with low cost. In this study, we applied a genome-wide association study (GWAS), by combining phenotyping and genotyping-by-sequencing (GBS), for the development of a single nucleotide polymorphism (SNP) marker set related to crown gall disease using 350 grapevine varieties. As a result of the GBS based genotyping analysis, about 58,635 SNPs were obtained. In addition, the phenotypic analysis showed 35.2% resistance, 73% moderate susceptibility and 16.4% highly susceptibility. Moreover, after confirmation, two genes (VvARF4 and VvATL6-like) were shown to be related to crown gall disease based on the results of GWAS analysis, using the phenotypic data, and GBS. High-resolution melting analysis (HRMA) was performed using the Luna® Universal Probe with real-time PCR to distinguish the melting peaks of the resistant and susceptible varieties. Our data show that these SNP markers are expected to be helpful in evaluating resistance against grapevine crown gall disease and in breeding.

Comparison of Flexural Strength of Three-Dimensional Printed Three-Unit Provisional Fixed Dental Prostheses according to Build Directions

  • Park, Sang-Mo;Park, Ji-Man;Kim, Seong-Kyun;Heo, Seong-Joo;Koak, Jai-Young
    • Journal of Korean Dental Science
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    • v.12 no.1
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    • pp.13-19
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    • 2019
  • Purpose: The aim of this study was to compare the flexural strength of provisional fixed dental prostheses which was three-dimensional (3D) printed by several build directions. Materials and Methods: A metal jig with two abutment teeth and pontic space in the middle was fabricated. This jig was scanned with a desktop scanner and provisional restoration was designed on dental computer-aided design program. On the preprocessing software, the build angles of the restorations were arranged at $0^{\circ}$, $30^{\circ}$, $45^{\circ}$, $60^{\circ}$, and $90^{\circ}$ and support was added and resultant structure was sliced to a thickness of $100{\mu}m$. Processed restorations were printed with digital light processing type 3D printer using poly methyl meta acrylate-based resin. After washing and post-curing, compressive loading was applied at a speed of 1 mm/min on a metal jig fixed to a universal testing machine. The maximum pressure at which fracture occurred was measured. For the statistical analysis, build direction was set as the independent variable and fracture strength as the dependent variable. One-way analysis of variance and Tukey's post hoc analysis was conducted to compare fracture strength among groups (${\alpha}=0.05$). Result: The mean flexural strength of provisional restoration 3D printed with the build direction of $0^{\circ}$ was $1,053{\pm}168N$; it was $1,183{\pm}188N$ at $30^{\circ}$, $1,178{\pm}81N$ at $45^{\circ}$, $1,166{\pm}133N$ at $60^{\circ}$, and $949{\pm}170N$ at $90^{\circ}$. The group with a build direction of $90^{\circ}$ showed significantly lower flexural strength than other groups (P<0.05). The flexural strength was significantly higher when the build direction was $30^{\circ}$ than when it was $90^{\circ}$ (P<0.01). Conclusion: Among the build directions $0^{\circ}$, $30^{\circ}$, $45^{\circ}$, $60^{\circ}$, and $90^{\circ}$ set for 3D printing of fixed dental prosthesis, an orientation of $30^{\circ}$ is recommended as an effective build direction for 3D printing.

Comparison of the Mechanical Properties between Bulk-fill and Conventional Composites (Bulk-fill 복합레진과 전통적 복합레진의 물성비교)

  • Noh, Taehwan;Song, Eunju;Park, Soyoung;Pyo, Aeri;Kwon, Yonghoon;Kim, Jiyeon;Kim, Shin;Jeong, Taesung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.43 no.4
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    • pp.365-373
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    • 2016
  • Composites are the most useful restorative material. However, composites have some disadvantages such as polymerization shrinkage, long working time, and susceptibility to water and contamination, which are stood out more especially when treating children. To solve these problems, bulk-fill composites have been developed. The aim of this study is to compare mechanical properties of bulk-fill and conventional composites. Bulk-fill composites (SureFil SDR flow (SDR), Tetric N-Ceram bulk fill (TBF)) and conventional composites (Filtek Z-350 (Z-350), Unifil Flow (UF), Unifil Loflo Plus (UL)) were used. The Vickers hardness tester was used to measure the microhardness of materials, and Fourier transform infrared spectroscopy was used to measure the degree of conversion. Polymerization shrinkage was measured by using a linometer. Flexural and compressive properties were measured by using the universal testing machine. Data were statistically analyzed by ANOVA and Scheffe's post hoc test. The level of significance was set to p < 0.05. Most conventional composites showed higher microhardness than bulk-fill composites. However, bulk-fill composites showed a higher top/bottom microhardness ratio than conventional composites. Bulk-fill composites showed a higher top/bottom degree of conversion ratio than conventional composites. The polymerization shrinkage was highest in UL and lowest in Z-350. The polymerization shrinkage of flowable composites was higher than that of non flowable composites. The compressive properties were highest in Z-350 and lowest in SDR and UL. In terms of flexural properties, Z-350 was the highest. However, none of the bulk-fill composites exhibited mechanical properties as good as those of conventional composites. Nonetheless, the ratio of microhardness and degree of conversion, which are important properties of bulk filling, were higher in bulk-fill composites. Therefore, the bulk-fill composites might be considered suitable restorative materials in pediatric dentistry.

Comparison of the Strain on the Alveolar Ridge According to the Occlusal Scheme of Complete Dentures (총의치 교합양식에 따른 응력 분포 양상 비교연구)

  • Choi, Won-Jun;Lim, Young-Jun;Kim, Chang-Whe;Kim, Myung-Joo
    • Journal of Dental Rehabilitation and Applied Science
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    • v.26 no.1
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    • pp.1-12
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    • 2010
  • The purpose of this study was to compare the strain on the alveolar ridge in the centric, eccentric and protrusive position according to the occlusal scheme (bilateral balanced occlusion with 33 degree anatomical teeth, group B; monoplane occlusion with non-anatomical teeth, group M; lingualized occlusion with 33 degree anatomical teeth and non-anatomical teeth, group L; of complete dentures. Experimental dentures were set bilateral balanced occlusion, lingualized occlusion and monoplane occlusion. They are analysed through T-Scan II(Tekscan, Boston, U.S.A) and 1.5mm thick layer was removed from the denture-supporting surface of resin model and then replaced with silicone to simulate resilient edentulous ridge mucosa. A $4{\times}6$ linear strain gauge is attached to the $1^{st}$ premolar and $1^{st}$ molar area. The strain values are recorded according to the occlusal scheme in the centric, eccentric and protrusive position after uniformly applying 50 N and 150 N force through a Universal Testing Machine(instron$^{(R)}$ 5567, Bluehill 2.0 software ,U.S.A.) with the models mounted in the articulator. When performing centric and protrusive occlusion, the three groups of occlusal scheme were compared in the anterior region and in the posterior region. The strains of each group were also compared in the working side and in the non-working side during eccentric excursion. It was observed that the strain in the bilateral balanced occlusion showed a higher value than the lingualized occlusion and monoplane occlusion in every position except the non-working side. However, during the eccentric movement the strain value in the non-working side showed the lowest value in the bilaterally balanced occlusion. The strain change amount from the working side or centric occlusion to non-working side and also the strain variation rate within the non-working side showed the highest value in bilateral balanced occlusion.

A Study on the Human Mind and Moral Mind Theory in Daesoon Thought (대순사상의 인심도심론(人心道心論) 연구)

  • Park Byung-mann
    • Journal of the Daesoon Academy of Sciences
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    • v.47
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    • pp.139-172
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    • 2023
  • This study aims to look into the concept of Human Mind and Moral Mind and the issue of their axiological interpretation in Daesoon Thought. In the Song dynasty, the concept of the Human Mind and Moral Mind was understood as indicating two aspects of the universal Human Mind. Discourse on this theory was undertaken by scholars such as Cheng Yichuan (程伊川), Su Shi (蘇軾), and Zhu Xi (朱熹). The differences between the Human Mind and Moral Mind were interpreted as issues of cultivation. The discussion of the Human Mind and Moral Mind were established through a systematic theory by Zhu Xi, and this developed into various forms of discourse and ideological stances thereafter. One of the most important issues of the Human Mind and Moral Mind theory was its axiological interpretation, which was divided largely into three patterns. One was to interpret the Human Mind and Moral Mind as evil and good respectively (proponents included Cheng Yichuan and Zhu Xi in his early theories), the other one saw them as value-neutral and good (proponents included Zhu Xi, Toegye 退溪, and Yulgok 栗谷), and the last one interpretation held them as pre-evil and pre-good (Dasan 茶山). As the Human Mind and Moral Mind can be seen as a universal human issue, the examination of those patterns in the Confucian tradition would be meaningful for understanding the Human Mind and Moral Mind as a theoretical base in Daesoon Thought. In Daesoon Thought, the Human Mind and Moral Mind are defined as private and public respectively, but no further explanation is provided regarding these items. If we infer by considering the two in the light of the overall ideology and values that Daesoon Jinrihoe pursues, the Human Mind can be said to represent basic biological desires such as clothing, food, and sexual satisfaction all of which are human vital activities needed for the preservation of the human race. The Moral Mind can be seen as a mind that is set upon practicing morality and realizing the ideological aims of 'supporting the nation and comforting the people,' 'vast saving all creatures,' and achieving 'harmony and peace for humankind.' However, the conscience and the selfish mind, which are related axiologically to the Human Mind and the Moral Mind, are defined respectively as good and evil and explained in a relatively systematic way which includes conceptual claims and details on the origin of these aspects of mind. The reason why the discussions of the conscience and selfish mind are more systematically described than the Human Mind and Moral Mind seems to be that issues relating to the conscience and selfish mind are more directly applicable to matters of religious doctrine.

Suggestions on the Types of the Distribution of Gardens for the Overseas Establishment of Traditional Korean Gardens - Oriented the Garden which is Applicable to the Open Space - (한국전통정원 해외조성을 위한 정원보급 유형 제안 - 공공 공간에 적용될 정원을 대상으로 -)

  • Kwon, Jin-Wook;Park, Eun-Yeong;Hong, Kwang-Pyo;Hwang, Min-Ha
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.31 no.3
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    • pp.106-113
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    • 2013
  • This study aims to establish the identity of traditional Korean gardens and develop a universal way for overseas Koreans and foreigners to have an appropriate understanding of traditional Korean gardens, as part of efforts to distribute and promote the overseas establishment of traditional Korean gardens. The focus of this study is on developing planning and design guidelines to ensure that traditional Korean gardens have individuality when they are established overseas and on establishing directional rules for planners. Although traditional Korean gardens may vary in form according to their purposes and spatial scales, the most important thing is that they should incorporate emotions that are well-matched with Korean landscapes and that their design language should be easily recognizable and understandable to everyone. The basic spatial types of traditional Korean gardens for overseas establishment, which are presented in this study, include the exhibition(fair) type, the garden type and the park type. These basic types serve as prototypes that correspond to the purposes of the gardens. In consideration of the spatial scale, the exhibition(fair) type is set as the minimum unit for composition, and suggested basic facilities include trees, a well, a pond and an island in the pond, flower beds and fences. The results of this study have significance as basic information for planning and designing traditional Korean gardens for overseas establishment.

Development of Organic Paste Porcelain for Fixed Prostheses (유기조성물을 이용한 페이스트형 일반도재 시스템)

  • Han, Jung-Suk;Lee, Myung-Hyun;Kim, Dae-Hyun;Chung, Hun-Young
    • Journal of Dental Rehabilitation and Applied Science
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    • v.20 no.2
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    • pp.109-120
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    • 2004
  • INTRODUCTION: The build-up method has been used for application of porcelain powder on the metal framework to make final tooth shape conventionally. This method takes time and need skill to mimic final shade and shape of porcelain fused to metal crown. The purpose of this study was to develop standard shape and shade laminating porcelain forms to reduce build-up time. METHODS: To make tooth form porcelain paste, several liquid organic compounds were added to conventional feldspathic porcelain. The amount of additives and rheologic property were tested to find out best composition. Comparison of mixing methods to reduced porosity, proper heating schedule, and measurement of shrinkage amount and residual organic materials were performed to set-up standard procedures. Finally, biaxial flexural strength and color of preformed laminated paste porcelain were compared with those of porcelain which fabricated by the conventional build-up method. RESULTS: There was no significant difference in physical properties and color stability between two fabrication methods after various testing methods. Conclusion: This new build-up method can be applied to fabricate the PFM crown and bridge without any loss of strength and optical properties.

Future Trend Impact Analysis Based on Adaptive Neuro-Fuzzy Inference System (ANFIS 접근방식에 의한 미래 트랜드 충격 분석)

  • Kim, Yong-Gil;Moon, Kyung-Il;Choi, Se-Ill
    • The Journal of the Korea institute of electronic communication sciences
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    • v.10 no.4
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    • pp.499-505
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    • 2015
  • Trend Impact Analysis(: TIA) is an advanced forecasting tool used in futures studies for identifying, understanding and analyzing the consequences of unprecedented events on future trends. An adaptive neuro-fuzzy inference system is a kind of artificial neural network that integrates both neural networks and fuzzy logic principles, It is considered to be a universal estimator. In this paper, we propose an advanced mechanism to generate more justifiable estimates to the probability of occurrence of an unprecedented event as a function of time with different degrees of severity using Adaptive Neuro-Fuzzy Inference System(: ANFIS). The key idea of the paper is to enhance the generic process of reasoning with fuzzy logic and neural network by adding the additional step of attributes simulation, as unprecedented events do not occur all of a sudden but rather their occurrence is affected by change in the values of a set of attributes. An ANFIS approach is used to identify the occurrence and severity of an event, depending on the values of its trigger attributes. The trigger attributes can be calculated by a stochastic dynamic model; then different scenarios are generated using Monte-Carlo simulation. To compare the proposed method, a simple simulation is provided concerning the impact of river basin drought on the annual flow of water into a lake.

A Study on the Successful Strategy for DAB : Focused on DAB Strategy in UK (DAB 성공전략에 관한 연구 - 영국 DAB전략을 중심으로 -)

  • Bae, Hong-Kyun
    • Korean Business Review
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    • v.18 no.2
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    • pp.133-149
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    • 2005
  • DAB is meeting with mixed success. Denmark is performing similarly to the UK thanks to the innovation of the public broadcaster but where DAB is not so strong, the problem can usually be pointed at the lack of enabling regulation. The UK's example, whilst not appropriate everywhere, has valuable lessons including licensing incentives for existing analogue commercial broadcasters and a market-building obligation on the national multiplex licensee. Despite the obvious success of DAB, it would be an omission to leave some of the continued criticisms unanswered, whether of its slow start or the underlying technology. True, there was much over-optimism in the mid-1990s, coupled with unrealistic promises and expectations of receiver pricing and consumer take-up. Governments across Europe have legislated for DAB digital radio in a variety of ways but few as successfully as the UK. It is essential that both public and private broadcasters are encouraged equally to participate in digital radio. The UK is fortunate because, for the last 10 years, there has been a progressive government policy towards digital broadcasting. The 1996 Broadcasting Act set out a full licensing regime for both digital television and digital radio. The 1996 UK legislation contained a number of key elements which have been cornerstones of its success. DAB digital radio began test broadcasting around ten years ago but it has not been a universal consumer success across Europe. In the UK, however, digital radio receivers are one of the fastest-selling consumer electronics products and sales have overtaken those of analogue radios. Why has the UK succeeded with DAB digital radio when other European countries have yet to see their markets take off? This article explains what steps the UK took to make DAB digital radio a success.

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Effects of Institution of Bankruptcy Proceedings on an Arbitration Agreement and Arbitral Proceedings (파산절차에 있어서의 중재합의의 효력과 중재절차)

  • Oh Chang-Seog
    • Journal of Arbitration Studies
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    • v.15 no.1
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    • pp.113-146
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    • 2005
  • Bankruptcy proceedings serve the purpose of the collective satisfaction of the debtor's creditors through the realisation of the debtor's assets and the distribution of the proceeds therefrom. Upon the adjudication bankruptcy, the debtor's right to administer and dispose of the property belonging to the bankruptcy estate shall be vested in the administrator. If a mutual contract was not or not completely fulfilled by the debtor and the other party at the time of the adjudication of bankruptcy, the administrator has right to choose wether to fulfil or terminate the contractual relation. Legal acts that have been conducted prior to the adjudication of bankruptcy and that are detrimental to the debtor's creditors may be contested by the administrator. However, these effects of bankruptcy will have not great influence on the arbitration agreement between the debtor and another party. An arbitration agreement that has been conducted prior to the adjudication of bankruptcy is binding the administrator as an universal legal successor of debtor. Only the arbitration agreement directly disadvantageous to the debtor's creditors may be contested by the administrator. Furthermore, it is not at the discretion of administrator whether or not to submit the dispute to arbitration because an arbitration agreement does not belong under the category of Art. 50 Korean bankruptcy Act which demands a mutual contract. Arbitral proceeding upon the property of the bankruptcy estate and pending for the debtor as plaintiff or against the debtor as defendant at the date of the adjudication of bankruptcy may be taken up at the given status by the administrator. This leads to a change of the party. If a duly summoned party fails to appear in arbitration court, the arbitrator, if satisfied there is no valid excuse, may continue the proceedings and make the award as if all the parties were present. This may be disadvantagious to the debtor's creditors because the arbitral award have the same effects on the participants as the final and conclusive judgement of the court. Even if there is a change of party on side of debtor to the administrator in bankruptcy, the arbitral proceedings will not be automatically postponed or suspended. The matter of how to proceed is at discretion of administrator, when the parties haven't agree on the arbitral proceedings. He can continue the arbitral proceedings without to grant an adjournment of hearing. However, an arbitration award may be challenged by a party dissatisfied and set aside by the court based upon the misconduct that violates the basic rights of the parties to a fair hearing. The arbitrator must treat the parties equally in the arbitral proceedings and give each party a full opportunity to present his case. The arbitrator, therefore, will carefully exercise his discretion in determining whether to continue the arbitral proceedings or to grant a postponing. In the practice, the arbitral proceedings may be usually postponed to grant due process.

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