• Title/Summary/Keyword: unique collection

검색결과 218건 처리시간 0.035초

시계열 군집분석을 통한 디지털 음원의 순위 변화 패턴 분류 (Derivation of Digital Music's Ranking Change Through Time Series Clustering)

  • 유인진;박도형
    • 지능정보연구
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    • 제26권3호
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    • pp.171-191
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    • 2020
  • 본 연구는 현대 사회에서 가장 가치 있는 문화자산이자 한류의 흐름에서 특히 중요한 위치를 차지하는 디지털 음악에 초점을 두었다. 디지털 음악에 대하여 공신력 있는 음원 차트인 '가온 차트'에 진입한 음원들의 73주간 순위 변화를 수집하였으며 유사한 특징을 가지는 패턴들로 분류하였다. 이후 각 순위 변화 패턴으로부터 주목할 만한 특징에 대한 설명적 분석을 수행하였다. 구체적으로 음원에 대한 신뢰도 이슈가 발생하기 이전 기간의 국내 발매된 디지털 음원들로 한정하여 시점을 일치시킨 후 시계열 군집분석을 통해 패턴을 도출하고자 하였다. 데이터 수집과 전처리를 통하여 742건의 중복되지 않는 음원들을 확보하였고, 시계열 순위 변화에 대한 시계열 군집분석 결과 16개의 패턴들이 도출되었다. 이후 도출된 패턴들을 기반으로 '스테디셀러'와 '원 히트 원더'의 두 가지 유형의 대표적인 패턴을 확인하였다. 나아가 두 패턴에 대하여 차트 내에서 음원의 생존 기간과 음원 순위에 관점에서 다섯 가지의 세분화된 패턴으로 분류하였다. 각 패턴들이 가지는 중요한 특징들은 다음과 같다. 원 히트 원더형 패턴에서 아티스트의 슈퍼스타 효과와 편승효과가 강하게 나타났으며, 소비자들의 디지털 음원 선택에 강한 영향을 미친다는 것을 확인하였다. 나아가 스테디셀러형 패턴을 통해서 매우 오랜시간 소비자들의 선택을 받는 음원들을 확인하였고, 소비자의 니즈를 관통하며 가장 많은 선택을 받는 음원들이 오히려 원 히트 원더형 패턴이 아니라 스테디셀러: 중기 패턴에 포진하고 있음을 확인하였다. 특히 주목할 만한 점은 스테디셀러형 패턴을 통해 기존의 패턴과는 상반되는 '차트 역주행' 현상을 확인했다는 것이다. 본 연구는 디지털 음원을 중심으로 상대적으로 소외되었던 분야인 시간의 흐름에 따른 음원의 순위 변화에 초점을 두었고, 음원의 흥행과 순위를 예측하는 것이 아니라 순위 변화의 패턴을 세분화함으로써 음원 연구에 대한 새로운 접근을 시도하였다는 점에서 의의가 있다.

무인도서의 지속가능한 관리를 위한 기본 정책방향 (Management Policy Directions for Sustainable Management of the Uninhabited Islands of Korea)

  • 남정호;강대석
    • 한국해양환경ㆍ에너지학회지
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    • 제8권4호
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    • pp.227-235
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    • 2005
  • 독특한 생태적 가치뿐만 아니라 관광수요 증가에 따른 잠재적 경제가치, 주권수호의 기점으로 기능하는 국가안보적 가치를 가지는 국토자원인 무인도서 생태계의 이용과 보전을 조화하기 위한 무인도서 관리 기본 정책방향을 제시하였다. 무인도서 현황과 관리 실태를 토대로 도출한 관리문제점은 무인도서 기초 자료의 부족과 낮은 정확도, 무인도서의 종합적 체계적 관리를 위한 기본 정책방향의 부재, 폐기물(쓰레기), 벌목, 채취, 동물방목, 외부생물에 의한 생태계 훼손과 교란, 무인도서 이용 개발수요의 합리적 수용을 위한 정책수단 미흡, 무인도서 관리 업무 수행에 필요한 인력과 재원의 부족 등 관리기반 취약, 무인도서의 특성을 반영하지 못하고 있는 법제도 등을 들 수 있다. 이러한 관리 문제점을 개선하기 위한 무인도서 관리 기본 정책방향을 무인도서 관리 기본방향, 관리전략, 법제도 개정 방향으로 나누어 제시하였다. 무인도서의 관리기본방향은 무인도서가 가지고 있는 독특한 생태적, 경제적, 주권수호 차원의 가치를 고려할 때 현재 및 미래의 이용 개발수요가 현재의 무인도서 생태계 가치를 훼손하지 않는 가치보전과 기존의 육상중심에서 육상과 해양을 하나의 관리단위로 설정하여 관리하는 '통합관리' 로 설정하였다. 무인도서 관리기본방향을 달성하고, 관리문제점을 효과적으로 해결하기 위한 전략으로 무인도서 종합조사를 통한 지식 기반 강화, 이용 개발 수요의 합리적 수용과 체계적 관리를 위한 무인도서 관리지침 개발 및 법제화, 환경현안 중 가장 문제가 되는 쓰레기의 효과적 처리를 위한 도서 쓰레기 광역수거처리 체계 구축, 관리능력 부족을 해결할 수 있도록 관리체제 개선을 통한 관리역량 강화 네 가지를 제시하였다. 무인도서 관리 기본 정책방향의 효과적인 이행을 위한 법제도 개선방향은 종합조사의 실시, 유형별 분류와 이응행위 제한사항 및 관리지침의 개발, 무인도서 관리 범위 재설정, 특정도서 관련 규정 정비 방안 등을 제시하였다.

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보건소 보건간호사의 지역사회 진단활동에 관한 조사연구 (A Study of community diagnosis activity by Community Health Nurse Working in Health Centers)

  • 조원정;김영란
    • 한국보건간호학회지
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    • 제6권1호
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    • pp.32-45
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    • 1992
  • An important role of community health nurses in health centers is to solve community health problems found through data collection methodology which has been used to identify the health needs of the community, diagnose the health problems and to plan health programs suitable for the health problems. Also community health nurses must be prepared to know the community health needs and to participate in the planning process. Since 1956 when the health center law was established, community health nurses have really implemented only the services which the government has asked them to do. This has kept them busy enough. But these days as society is in rapid change, community health nurses should have the flexibility to deal with the social change and demands that are unique to their community each which has different health needs and demands. So community health nurses need to identify what community health problems exist in their particular communities. The purposes of this study were as follows. 1) To explore the suitability of the health programs which the government has asked the community health nurses to do for their own communities and if these programs are not suitable, to explore the reasons why. 2) To explore the degree to which the community health nurses have the ability to identify health problems in their own communities and activate the community diagnostic process. 3) To identify the degree that the community health nurses have the ability to implement plans related to community diagnosis. 4) To find out how much data related to community health problems, the community health nurses have and how they are utilizing it. 5) To measure the community health nurses self-confidence concerning diagnostic activities for community health. The study subjects were 454 Community Health Nurses working in Health Centers in Seoul, Korea. The period of data collection was 6 days(Nov. 9th 1991-Nov. 15th 1991). A questionnaire used for data collection was composed of three different items; general characteristics, community health diagnostic activities and self-confidence in performing diagnostic activities. The results of the study are as follows. First, over one third of the respondents replied that the government required activities for their communities are not appropriate. Of these activities the most frequent reply $(51.2\%)$ indicated that many of the activities in the community were inappropriate to the actual situation. Further, $25\%$ of the replies indicated that many activities were only administratively oriented and as such not appropriate. Second, $49.8\%$ of the respondents replied that they had done general assessments and had a general idea of the health problems of their community. Effective solutions to health problems could be found with an increase in health personnel and management ability according to $41.5\%$ of the respondents. Third, to the question as to whether they had ever independently implemented a plan towards solving community diagnosed problems, $52\%$ of nurses replied 'never', $40\%$ 'occasionally' but only $7.5\%$ replied that they did it frequently. Actually there was very little done even in the basic work of collecting the necessary data. Fourth, when asked how much of basic information they had collected that might be used in community diagnosis activity, of 26 items in 5 areas, there was hardly one for which complete data had been collected. Fifteen percent did have data on the geographical aspects of their area, housing distribution and types of housing, while $17.8\%$ knew the frequency with which the health center was used. Concerning community resources, even with a list of community resources, only $12.3\%$ had data on any of these resources, and this data was incomplete. Further, information about social work institutions, and facilities was also incomplete, only $14.2\%$ of the respondents had any data and even it was incomplete; that is, in general, the nurses did not have this information. Fifth, concerning the confidence of the community health nurse in their ability to carry out community diagnoses activities, $60\%$ replied that they were very or at least nominally confident, indicating that although they were not doing community diagnostic activities they felt they could do so, as they were carrying out home visits and program planning as part of their official duties. The following recommendations are made based on the results of this study. First; since the community health nurses have a high perception of the need for community diagnostic activities and. high confidence in their ability to carry out this activity and high percentage of respondents replied that with a little training they could do this even better it is recommended that community diagnostic activity training be included in the continuing education program for community health nurses. Second, in order for the Community Health Nurses to successfully solve the health problems of their respective community they reported to a need to increase the number of health personnel, improve the facilities and the system of managing their work. Considering this, it is recommended that ways be sought to remedy these deficits.

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벨기에 왕립예술역사박물관 소장 고려시대 금동침통의 과학적 보존처리를 통한 제작기법 연구 (Study of the Production Techniques Used in the Goryeo-period Gilt-Bronze Case for Acupuncture in the Collection of the Royal Museums of Art and History, Belgium)

  • 이재성;박영환
    • 박물관보존과학
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    • 제27권
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    • pp.147-164
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    • 2022
  • 전 세계 22개국에 흩어져 있는 우리 문화재는 20만 여점에 이른다. 저마다 다른 사연을 가지고 외국에 나가게 된 우리 문화재 중 일부는 해외 기관에 소장되어 세계인들에게 우리 문화를 알리고 있지만 훼손되어 전시되지 못하는 경우도 있다. 이와 같은 문제를 해결하기 위해 2013년부터 '국외 소재 한국문화재 보존·복원 및 활용 지원 사업'을 추진하고 있다. 2021년은 한국과 벨기에 수교 120주년을 기념하여 벨기에 왕립예술역사박물관 소장 고려시대 금동침통을 국내에 들여와 보존처리했다. 보존처리의 기본 방향은 원형을 보존하고 부식이 지속되는 것을 최대한 늦추는 것이기 때문에 표면 부식물제거, 안정화처리, 강화처리를 거치는 기본적인 보존처리 순서로 진행하였다. 하지만 국외 소재 한국문화재 보존처리 지원 사업 중 금속문화재로서는 처음으로 보존처리된 사례이기 때문에 국외 기관과의 차별화가 필요했다. 이를 위해 X선 투과조사, 컴퓨터단층촬영, 3차원 현미경 조사 등 과학적인 조사·분석법으로 고려시대의 다양한 금속 공예기술을 파악하였다. 표면에 정교하게 새겨진 연꽃, 넝쿨 등의 다양한 문양은 끝이 둥근 정을 이용해 점선으로 시문했다. 또한 문양이 새겨진 구리판을 원통형으로 말기 위해서 양끝을 약 2~3mm 정도 겹쳐지게 은땜으로 접합하였으며, 겹친 부위의 단차가 거의 없을 정도로 평평하게 단접하였다. 제작공정의 마지막 과정에서는 금분을 이용한 아말감 도금법으로 표면을 화려하게 도금하였다. 국외 소재 한국문화재인 벨기에 왕립예술역사박물관 소장 금동침통에 대한 보존처리로 원형을 보존하고, 더 이상의 추가적인 부식을 예방하였다. 특히 국외 소재 한국문화재에 대한 과학적 조사를 통해 역사적 가치와 학술적 가치를 되살릴 수 있었다.

여성건강을 위한 개념적 모형 (Conceptual Model for Women s Health)

  • 이경혜
    • 대한간호학회지
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    • 제27권4호
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    • pp.933-942
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    • 1997
  • There has recently been an increased interest in women's health from, various disciplines, with different perspectives presented according to each profession's academic background. This has led to many instances of incorrectly defining, or misinterpretation, of the issues even among professionals. Nurse scholars as well as practitioners who work in women's health care need to have a clear conceptual understanding of women's health in order to build a body of knowledge, delineate curricular activities, and set directions for professional nursing interventions. In addition, a conceptual model that may be directly utilized in practice is needed to maintain and promote women's health issues. The purpose of this study was to apply a Hybrid model, analyzing conceptual definitions and discussions related to women's health gathered from review of the literature. Further to compare analyticals the concepts and properties observed from field work, so as to present a final definition of women's health and, build a conceptual framework for a united comprehensive perspective on the concept as well as on nursing practice. Data collection and analysis consisted of a theoretical stage, field work stage, and final analysis. A heterogeneous group of professionals and lay persons, 39 in all, participated in the field work. Study findings Include several subconcepts under the concept of women's health : a woman's whole life, holistic health, quality of life, awareness of being a woman, individual nursing, self care ability, reproductive health, and family health. Thus, a comprehensive definition was built, 1. e., "Women's health care be defined as improvement in the quality of life of women through attainment of holistic health throughout the life span. With reproductive health at the core, the concept is directly related to family and national health, and includes taking care of one's own health based on awareness of being a woman and utilizing self care activities. Women's health care issues are unique and allow various responses, therefore women's health professionals need to apply individual approaches to reach solutions in attaining holistic health and improving quality of life." The constructual factors of women's health were found to be reproductive functions, diseases more common in woman, self actualization, mental health, women's health policies, sexuality, midlife changes, and marital relations, with each factor having more than three properties. Positive factors affecting women's health were found to be a normal childbearing process, a healthy lifestyle, active health management, health information, support, and resources, and interpersonal relationships. Negative factors were found to be overwhelming role stress, cultural oppression, gender inequality, distorted sexual identity, economic difficulties, misuse and/or abuse of substances, and stress. The model of women's health may be visualized as a balance scale set upon a woman's life, supporting 4 concentric circles. The innermost circle and second circle incorporate conceptual definitions of women's health, and the outer two circles represent the constructional factors and properties of women's health. Each circle has its own color that symbolizes the conceptual meaning. Positive and negative factors are represented as weights at either end of the scale, and are affected by nursing intervention, i. e., health and wellness increase when positive factors are stronger, whereas disease and illness increase when negative factors are stronger. This model is only a preliminary effort and requires much discussion and testing to be further developed. Continuous research is also required.

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중등 과학영재 지도교사의 수업 전문성에 관한 사례연구 (A Case Study on Teachers' Teaching Professionalism for Secondary Science-Gifted Students)

  • 배미정;김희백
    • 한국과학교육학회지
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    • 제30권4호
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    • pp.412-428
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    • 2010
  • 이 연구는 2명의 중등 과학영재 지도 교사의 수업 전문성을 교수지향의 관점에서 알아보고자 한 것이다. 두 교사 모두 6년 이상 중등 과학영재의 생물 수업을 담당하였으며, 영재교육 교사연수를 이수하였다. 참여교사 당 2차시의 수업관찰과 녹화, 2차례의 심층면담을 통해 자료를 수집하였다. 모든 수업자료와 면담자료는 전사된 후 분석되었다. 연구결과 교사들의 수업에서 몇 가지 특징이 나타났다. 두 교사 모두 '개방적 탐구' 지향을 나타냈다. 이것은 탐구력과 창의적 문제 해결력을 신장시켜 과학영재가 과학자로 성장할 수 있도록 도와야 한다는 교사의 교육목표와 신념을 반영한 것이다. 그러나 교사의 전문적 지식의 구체적 내용과 강조점의 차이에 따른 신념은 '학문적 엄격성'과 '발견'의 서로 다른 지향으로 나타나기도 하는데, 이것은 수업에서 다루는 교과 내용 수준이나 범위 등에 영향을 주는 것으로 보인다. 교사들의 과학교수지향은 수업 대상 학생과 과학영재 수업 경험의 증가에 따라 변화한 것으로 나타났다. 이 과정에서 교사의 과학영재 교육철학이 교수지향에 중요한 영향을 끼치는 것으로 나타났다. 교사의 전문성 신장을 위한 지속적인 노력을 바탕으로, 교사는 수업에 대한 자기반성을 통해 과학영재교육의 이론적 지식을 비판적으로 검토한다. 이를 통해 교사의 과학영재 교육철학이 정립되고 내면화될 때, 교사가 의도한 과학교수지향이 수업 실행에서도 일치되게 나타나는 것으로 보인다.

현대 니트패션에 나타난 혼합현상 - 2000년~2008년 여성컬렉션을 중심으로 - (A Mixture Phenomena Expressed in Contemporary Knit Fashion - Focus on Woman Collection from 2000 to 2008 -)

  • 박문희;이연희
    • 한국의류학회지
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    • 제33권12호
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    • pp.1924-1934
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    • 2009
  • 현대사회의 혼합화 현상과 니트웨어의 수요 증가에 따른 독특한 질감이 요구되어지고 있어 이에 따른 전략적인 방안들이 절실히 요구되고 있다. 본 연구는 세계 4대 컬렉션의 2000~2008년의 여성컬렉션의 횡편 니트 3~8게이지의 자료를 선정, 현대 니트패션에 나타난 혼합현상을 출현 빈도와 표현특성으로 분석하였다. 혼합현상은 상 하위문화의 혼합, 남 여 성의 혼합, 스타일의 혼합, 공간의 혼합, 타소재의 혼합, 기능의 혼합의 순으로 나타났으며, 2004년에 가장 높은 빈도수를 나타냈다. 혼합현상의 표현특성에서 가장 높은 빈도수를 나타낸 상 하위문화의 혼합은 고급스러운 소재, 고상한 무늬와 색상의 상 하위개념의 경계성의 모호와 남 여 성의 혼합은 기능성과 편리성의 추구와 양성성의 공유로 특성을 나타냈다. 스타일의 혼합은 니트패션의 전통적인 형태에서 정형과 비정형의 스타일의 혼합으로 특성을 나타냈고, 공간의 혼합은 니트의 역사가 깊은 북유럽의 전통적 니트문양이 현대적인 요소와 혼합된 특성을 나타내고 있었다. 타소재의 혼합은 장식적인 소재와 본래의 기능적인 용도를 초월하여 부분적으로 혼합되었고, 기능의 혼합은 두 가지 아이템의 용도로 활용 가능한 특성을 나타냈다. 이와 같이 현대 니트패션에 나타난 혼합 현상은 타영역의 혼합으로 상상력이 더해져 그 표현 가능성의 범위가 점차 확대되어 가고 있다.

"침구자생경(鍼灸資生經)"의 침구 문헌적 특징에 관한 연구 (A Study about Characteristics of literature of acupuncture and moxibustion in "Chimgujasaenggyeong(鍼灸資生經)")

  • 박현국;김기욱
    • 대한한의학원전학회지
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    • 제21권4호
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    • pp.61-74
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    • 2008
  • The acupuncture and moxibustion documentary characteristics of "Chimgujasaenggyeong" can be summarized into 6 parts such as the follwing 1. "Chimgujasaenggyeong" was written at about 1180-1195 during the Southern Song period. It contains 7 volumes in all. The acupuncture points and their variations in volume 1 were all directly recorded from Wang-yuil(王維一)'s "Dong-insuhyeolchimgudogyeong(銅人腧穴鍼灸圖經)" and 11 points were added from volumes 99 and 100 of "Taepyeongseonghyebang(太平聖惠方)", bringing the total to 365 points, which was different from the 360 that the people knew. Volume 2 is the actual collection of theses on acupuncture and moxibustion by Wangjipjung(王執中) and shows his unique views on the basic problems of acupuncture and moxibustion such as selection of points[取穴], application of moxas[施灸], aftercare of moxibustion[灸後護理] and acupuncture and moxibustion contraindications[鍼灸禁忌]. Volumes 3${\sim}$7 mostly divide the indications(主治) from "Dong-insuhyeolchimgudogyeong", "Taepyeongseonghyebang", "Cheon-geumyobang(千金要方)" by disease into chapters. 2. Of the remaining editions the 'Cheonryeok Guanggeunseodang Inbon(天曆 廣勤書堂 印本)' of the Won dynasty is the first, and the Jeongtong(正統) new edition is a reprint based on the Cheonryeok(天曆) edition, and the Jeongtong edition reprinted in the 9th year of Guanmun(寬文) of Japan has many missing and wrong characters compared to the original copy. 3. The big letters[大字] under the line in the current editions are all postscripts[按語] of Wangjipjung and the 5 verses quoted from other books that do not have their origin listed and have the qualities of rules for treatment in the first chapter of volume 3 'Heoson(虛損)' were put together by Wang. 4. In the annotations in small print of the current edition of "Jasaenggyeong" there are Wisegeol(衛世傑)'s added annotations in addition to Wangjipjung's original ones. 5. Some of the many medical books quoted by the "Jasaenggyeong" that are from before the Song dynasty have been lost completely and only can be seen here in this important text. 6. The quotations said to be from 'Myeongdanggyeong(明堂經)'(or 'Myeongdang(明堂)', 'Myeong(明)') in "Jasaenggyeong" are directly from volume 77 'Chimgyeong(鍼經)' and volume 100 'Myeongdang' of "Taepyeongseonghyebang" and not another book. The quotes from 'Myeongdang' in accupuncture and moxibustion books after the Song dynasty were directly or indirectly copied from "Jasaenggyeong".

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한국인 영아초기 수유시 모아상호작용 행동형태에 관한 현상학적 연구 (A Phenomenological Study on Mother-Infant Interacting Behavior Patterns Related to Newborn Infant Feeding in Korea)

  • 한경자
    • 대한간호학회지
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    • 제21권1호
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    • pp.89-116
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    • 1991
  • The purpose of this study was to describe mother -infant interacting behavior patterns related to newborn infant feeding and to explore the mother's cultural belief about their infant. The data collection was conducted by observation and interview. Twenty-five mothers and their newborn infants who were normally delivered and were also planned to breast feed were comprised as the subjects of this study. All subjects were interviewed and observed individuaily at 1 to 5 days after the delivery at the hospital, mid -wife's clinic, Maternal Child Health Center and their home throughout the country from remote area to big city, The observation data were recorded with symbolic letter on a recording sheet newly developed as a result of preliminary study. The interview data were taperecorded and then recorded in narrative form. Mother - infant interaction behaviors in early feeding period were analyzed based on 19 analytic sub-categories and their composing elements. Unit of analysis were mother, infant and mother -infant dyad. 8 analytic categories draw from the data. Each were preparation, instrument, interaction inducing, evaluation referred to mother's behavior, preparation, instrument, interaction inducing referred to infant's behavior and synchronic behaviors referred to mother - infant dyad. Frequencies of behavior items based on the categories were converted to percent. The result showed that in mother's preparation behavior, the breast condition of Korean mother can be an affecting factor for mother - infant interaction during feeding, and vocalization behavior was observed most frequently in interaction inducing behavior while the least frequent behavior observed was contacting. Subcultural characteristics of mother - infant interaction behaviors were analyzed for their relationships between groups of mothers who have lived in remote area vs urban area, and who were multipara vs primipara. Using a chi -square test, there were statistically significant relationships in the activity of psychological readiness in preparation behavior and the movement of extremities for the position of instrumental behavior in both groups. However, interaction inducing behaviors were not related with statistical significance in any set of groups. Accomplishment of marriage, bonding and emotional mediation of family members were the categories related to mother's cultural belief about the infant in aspect of functional values. Infant at birth is considered little more than a biological organism without social capabilities. Although the newborn infant is still be attached to his mother, he makes his mother extend her territoriality. The mother's interacting behavior toward her infant based on those beliefs appeared task oriented, separative behavioral series. On the other hand, it was seen that infant reacted independently to his mother's behavior by the in-nate perceptual abilities. Those independent behavioral series of mother and infant on the feeding situation were synchronized at any moment. Nurses are In a unique position to teach mothers about their infant's capabilities and help reducing some of uncertainty about infant's behaviors. Study results indicated that the informations infant's social capabilities and breast feeding should be given to the mothers. The results of this study have several implications for nursing. First, the study results will be used as fundamental resources for the development of the assessment tool about the early mother - infant interaction. Second, the results could be a relevant information in the fied. I of maternal child nursing education as real and useful data. Third, the behavioral patterns of early mother - infant interaction which were classified based on the qualitative analysis could be used for nursing theory development as very fundamental data.

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Genomic DNA Chip: Genome-wide profiling in Cancer

  • 이종호
    • 한국생물정보학회:학술대회논문집
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    • 한국생물정보시스템생물학회 2001년도 제2회 생물정보 워크샵 (DNA Chip Bioinformatics)
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    • pp.61-86
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    • 2001
  • All cancers are caused by abnormalities in DNA sequence. Throughout life, the DNA in human cells is exposed to mutagens and suffers mistakes in replication, resulting in progressive, subtle changes in the DNA sequence in each cell. Since the development of conventional and molecular cytogenetic methods to the analysis of chromosomal aberrations in cancers, more than 1,800 recurring chromosomal breakpoints have been identified. These breakpoints and regions of nonrandom copy number changes typically point to the location of genes involved in cancer initiation and progression. With the introduction of molecular cytogenetic methodologies based on fluorescence in situ hybridization (FISH), namely, comparative genomic hybridization (CGH) and multicolor FISH (m-FISH) in carcinomas become susceptible to analysis. Conventional CGH has been widely applied for the detection of genomic imbalances in tumor cells, and used normal metaphase chromosomes as targets for the mapping of copy number changes. However, this limits the mapping of such imbalances to the resolution limit of metaphase chromosomes (usually 10 to 20 Mb). Efforts to increase this resolution have led to the "new"concept of genomic DNA chip (1 to 2 Mb), whereby the chromosomal target is replaced with cloned DNA immobilized on such as glass slides. The resulting resolution then depends on the size of the immobilized DNA fragments. We have completed the first draft of its Korean Genome Project. The project proceeded by end sequencing inserts from a library of 96,768 bacterial artificial chromosomes (BACs) containing genomic DNA fragments from Korean ethnicity. The sequenced BAC ends were then compared to the Human Genome Project′s publicly available sequence database and aligned according to known cancer gene sequences. These BAC clones were biotinylated by nick translation, hybridized to cytogenetic preparations of metaphase cells, and detected with fluorescein-conjugated avidin. Only locations of unique or low-copy Portions of the clone are identified, because high-copy interspersed repetitive sequences in the probe were suppressed by the addition of unlabelled Cotl DNA. Banding patterns were produced using DAPI. By this means, every BAC fragment has been matched to its appropriate chromosomal location. We have placed 86 (156 BAC clones) cytogenetically defined landmarks to help with the characterization of known cancer genes. Microarray techniques would be applied in CGH by replacement of metaphase chromosome to arrayed BAC confirming in oncogene and tumor suppressor gene: and an array BAC clones from the collection is used to perform a genome-wide scan for segmental aneuploidy by array-CGH. Therefore, the genomic DNA chip (arrayed BAC) will be undoubtedly provide accurate diagnosis of deletions, duplication, insertions and rearrangements of genomic material related to various human phenotypes, including neoplasias. And our tumor markers based on genetic abnormalities of cancer would be identified and contribute to the screening of the stage of cancers and/or hereditary diseases

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