• Proceedings of the Korean Society of Embryo Transfer Conference
• /
• 2002.11a
• /
• pp.78-78
• /
• 2002

The role of heat shock proteins in shielding organism from environmental stress is illustrated by the large-scale synthesis of these protein by the organism studied to date. However, recent evidence also suggests an important role for heat shock protein in fertilization and early development of mammalian embryos. Effects of elevated in vitro temperature on in vitro produced bovine embryos were analysed in order to determine its impact on the expression of heat shock protein 70 (HSP70) by control and frozen-thawed after in vitro fertilization (IVF) or nuclear transfer (NT). The objective of this study was to assess the developmental potential in vitro produced embryos with using of the various containers and examined expression and localization of heat shock protein 70 after it's frozen -thawed. For the vitrification, in vitro produced embryos at 2 cell, 8 cell and blastocysts stage after IVF and NT were exposed the ethylene glycol 5.5 M freezing solution (EG 5.5) for 30 sec, loaded on each containers such EM grid, straw and cryo-loop and then immediately plunged into liquid nitrogen. Thawed embryos were serially diluted in sucrose solution, each for 1 min, and cultured in CRI-aa medium. Survival rates of the vitrification production were assessed by re-expanded, hatched blastocysts. There were no differences in the survival rates of IVF using EM grid, cryo-loop. However, survival rates by straw were relatively lower than other containers. Only, nuclear transferred embryos survived by using cryo-loop. After IVF or NT, in vitro matured bovine embryos 2 cell, 8 cell and blastocysts subjected to control and thawed conditions were analysed by semiquantitive reverse transcription polymerase chain reaction methods for hsp 70 mRNA expression. Results revealed the expression of hsp 70 mRNA were higher thawed embryos than control embryos. Immunocytochemistry used to localization the hsp70 protein in embryos. Two, 8-cell embryos derived under control condition was evenly distributed in the cytoplasm but appeared as aggregates in some embryos exposed frozen-thawed. However, under control condition, blastocysts displayed aggregate signal while Hsp70 in frozen-thawed blastocysts appeared to be more uniform in distribution.

• Journal of the Korean Ceramic Society
• /
• v.40 no.10
• /
• pp.967-972
• /
• 2003

The inner-diameter 5 cm cold hollow cathode ion source was designed for the high current density and the homogeneous beam profile of ion beam. The ion source consisted of a cylindrical cathode, a generation part of magnetic field, a plasma chamber, convex type ion optic system with two grid electrode, and DC power supply system. The cold hollow cathode ion sources were classified into standard type (I), electron output electrode modified type (II). The operation of the ion source was done with discharge current, ion beam potential and argon gas flow rate. The modification of electron output electrode resulted in uniform plasma generation and uniform area of ion beam was extended from 5 cm to 20 cm. Improved ion source was evaluated with beam uniformity, ion current, team extraction efficiency, and ionization efficiency.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.22 no.6
• /
• pp.860-873
• /
• 1998

In a low speed open-type wind tunnel, a group of parallel wakes downstream of two dimensional grid model consisting of several circular cylinders were experimentally investigated to study the response of the wake flows to the acoustic excitation, in hoping to promote the understanding of the underlying mechanism behind the gross flow change due to artificial excitation. In the unexcited wake flows, the development of the individual wakes behind cylinders was almost uniform for the ratio of the spacing to the cylinder diameter of s/d.geq.1.5. For smaller s/d, however, the jet streams issued through the gaps between the cylinders became biased in one side and the cylinders had wakes of different sizes. At s/d=1.25, the gap flow directions change in time, leading to unstable wake patterns. Further reduction in s/d made this unstable flip-flopping of the jets stable. The most effective excitation frequency was found to be in the Strouhal number range of St=0.5-0.6. This frequency was related to the vortex shedding. At s/d=1.75, the excitation frequency was 2 or 4 times the vortex shedding frequency. When the flow was excited at this frequency, the vortex sheddings were energized, and pairings between neighboring vortices were generated. Also, the merging process between individual wakes was accelerated. The unstable and unbalanced wake patterns at s/d=2.15 were made stable and balanced. The unstable and unbalanced wake patterns at s/d=2.15 were made stable and balanced. For smaller spacing of s/d .leq,1.0, the acoustic excitation became less effective in controlling the flow.

• Journal of Korea Spatial Information System Society
• /
• v.6 no.2 s.12
• /
• pp.51-62
• /
• 2004

Recently, the location-based service for moving customers is becoming one of the most important service in mobile communication area. For moving object applications, there are lots of update operations and such update loads are concentrated on some particular area unevenly. The primary processing of LBS application is spatio-temporal range queries. To improve the throughput of spatio-temporal range queries, the time of disk I/O in query processing should be reduced. In this paper, we adopt non-uniform two-level grid index structures of GALIS architecture,which are designed to minimize update operations. We propose query scheduling technique using spatial relationship and time relationship and a combined spatio-temporal query processing method using time zone concepts to improve the throughput of query processing. Some experimental results are shown for range queries with different query range to show the performance tradeoffs of the proposed methods.

• Journal of the Korean Vacuum Society
• /
• v.18 no.3
• /
• pp.164-175
• /
• 2009

CFD is used to analyze gas flow characteristics, power absorption, electron temperature, electron density and chemical species profile of an internal antenna type inductively coupled plasma system. An optimized grid generation technology is used for a complex real-scale models for industry. A bare metal antenna shows concentrated power absorption around rf a feeding line. Skin depth of power absorption for a system is modeled to 50 mm, which is reported 53 mm by experiments. For an application of bipolar plates for hydrogen fuel cells, multi-sheet loading ICP nitriding system is proposed using an internal ICP antenna. It shows higher atomic nitrogen density than reported simple pulsed dc nitriding systems. Minimum gap between sheets for uniform nitriding is modeled to be 39 mm.

• Journal of computational fluids engineering
• /
• v.16 no.4
• /
• pp.39-46
• /
• 2011

A numerical study of a turbulent natural convection in an enclosure with the lattice Boltzmann method (LBM) is presented. The primary emphasis of the present study is placed on investigation of accuracy and numerical stability of the LBM for the turbulent natural convection flow. A HYBRID method in which the thermal equation is solved by the conventional Reynolds averaged Navier-Stokes equation method while the conservation of mass and momentum equations are resolved by the LBM is employed in the present study. The elliptic-relaxation model is employed for the turbulence model and the turbulent heat fluxes are treated by the algebraic flux model. All the governing equations are discretized on a cell-centered, non-uniform grid using the finite-volume method. The convection terms are treated by a second-order central-difference scheme with the deferred correction way to ensure accuracy and stability of solutions. The present LBM is applied to the prediction of a turbulent natural convection in a rectangular cavity and the computed results are compared with the experimental data commonly used for the validation of turbulence models and those by the conventional finite-volume method. It is shown that the LBM with the present HYBRID thermal model predicts the mean velocity components and turbulent quantities which are as good as those by the conventional finite-volume method. It is also found that the accuracy and stability of the solution is significantly affected by the treatment of the convection term, especially near the wall.

• Wind and Structures
• /
• v.26 no.5
• /
• pp.331-341
• /
• 2018

A numerical study on the flow over a square cylinder in the vicinity of a wall is conducted for different Couette-Poiseuille-based non-uniform flow with the non-dimensional pressure gradient P varying from 0 to 5. The non-dimensional gap ratio L (=$H^{\ast}/a^{\ast}$) is changed from 0.1 to 2, where $H^{\ast}$ is gap height between the cylinder and wall, and $a^{\ast}$ is the cylinder width. The governing equations are solved numerically through finite volume method based on SIMPLE algorithm on a staggered grid system. Both P and L have a substantial influence on the flow structure, time-mean drag coefficient ${\bar{C}}_D$, fluctuating (rms) lift coefficient ($C_L{^{\prime}}$), and Strouhal number St. The changes in P and L leads to four distinct flow regimes (I, II, III and IV). Following the flow structure change, the ${\bar{C}}_D$, $C_L{^{\prime}}$, and St all vary greatly with the change in L and/or P. The ${\bar{C}}_D$ and $C_L{^{\prime}}$ both grow with increasing P and/or L. The St increases with P for a given L, being less sensitive to L for a smaller P (< 2) and more sensitive to L for a larger P (> 2). A strong relationship is observed between the flow regimes and the values of ${\bar{C}}_D$, $C_L{^{\prime}}$ and St. An increase in P affects the pressure distribution more on the top surface than on bottom surface while an increase in L does the opposite.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.30 no.9A
• /
• pp.722-728
• /
• 2005

In this paper, the reverse-link performance of synchronous DS-CDMA cellular networks is investigated in Rician multipath fading environments. The system's performance is evaluated in terms of the achievable average bit error rate BER) and the user capacities of two different network layouts, namely those of a uniform grid of hexagonal multiple cells and a single isolated cell. In the multiple-cell scenario, the impact of the other cells' interference on the attainable capacity of the synchronous DS-CDMA uplink is investigated. Upon comparing both networks to a conventional asynchronous CDMA system, we demonstrate an achievable user capacity gain of $25\%$ to $56\%$ for synchronous uplink transmissions over that of the corresponding asynchronous transmission scenario at BER = $10^{-3}$.

• Journal of the Society of Naval Architects of Korea
• /
• v.32 no.3
• /
• pp.72-82
• /
• 1995

In the present paper, flow characteristics and free surface waves generated by a submerged hydrofoil advancing with an uniform speed are calculated. Using a numerical method based on a MAC(Marker And Cell) method, the Navier-Stokes and the continuity equations are solved to simulate flow fields around the hydrofoil. Computations are carried out in a rectangular grid system in which grids are concentrated near the foil and the free surface to improve numerical accuracies. Viscous flow phenomenas including pressure distributions are computed. Moreover, the influences of submerged depths upon the generated wave profiles and the wave breaking phenomena are also investigated. Experiments are performed at the towing tank of Inha University to measure free surface wave elevations due to the advancing hydrofoil. The computational results are compared with the present and the other available experimental data to show the accuracy of the numerical method developed.

• Journal of the Institute of Electronics Engineers of Korea TE
• /
• v.42 no.1
• /
• pp.35-40
• /
• 2005

Recently, the location based service for moving customers is becoming one of the most important service in mobile communication area and for moving object applications, there are lots of update operations and such update loads are concentrated on some particular area unevenly. The primary processing of LBS application is spatio-temporal range queries and to improve the throughput of spatio-temporal range queries, the time of disk I/O in query processing should be reduced. In this paper, we adopt non-uniform two-level grid index structure, which are designed to minimize update operations. We propose query scheduling technique using spatial relationship and time relationship and a combined spatio-temporal query processing method using time zone concepts to improve the throughput of query processing. Some experimental results are shown for range queries with different query range to show the performance tradeoffs of the proposed methods.