• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.6
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• pp.331-334
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• 2002

Culture conductivity and on-line NADH fluorescence were used to measure cellular growth in plant cell suspension cultures of Podophyllum hexandrum. An inverse correlation between dry cell weight and medium conductivity was observed during shake flask cultivation. A linear relationship between dry cell weight and culture NADH fluorescence was obtained during the exponential phase of batch cultivation In a bioreactor under the pH stat (pH 6) conditions. It was observed that conductivity measurement were suitable for biomass characterisation under highly dynamic uncontrolled shake flask cultivation conditions. However, if the acid/alkali feeding is done for pH control the conductivity measurement could not be applied. On the other hand the NADH fluorescence measurement allowed online-in situ biomass monitoring of rather heterogenous plant cell suspension cultures in bioreactor even under the most desirable pH stat conditions.

• Journal of Mechanical Science and Technology
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• v.17 no.9
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• pp.1332-1338
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• 2003

To estimate the true wear rate of polyethylene acetabular cups used in total hip arthroplasty, the dynamic compressive creep deformation of ultra-high molecular weight polyethylene (UHMWPE) was quantified as a function of time, load amplitude, and radial location of the specimen in the extruded rod stock. These data were also compared with the creep behavior of polyethylene observed under static loading. Total creep strains under dynamic loading were only 64％, 70％, and 61％ of the total creep strains under static loading at the same maximum pressures of 2 MPa,4 MPa, and 8 MPa, respectively. Specimens cut from the periphery of the rod stock demonstrated more creep than those cut from the center when they were compressed in a direction parallel to the extrusion direction (vertical loading) whereas the opposite was observed when specimens were compressed in a direction perpendicular to the extrusion direction (transverse loading). These findings show that creep deformation of UHMWPE depends upon the orientation of the crystalline lamellae.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.27 no.10
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• pp.1636-1643
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• 2003

In this study, bolted joint made of Glass-Mat Reinforced Thermoplastics (GMT) specimen was under tensile loading to investigate the relation between joint strength and glass-fiber weight fraction of the flat plate specimen. The effect of molding conditions such as the initial size of a GMT charge and molding temperatures was investigated under plane strain condition. In consideration of the specimen geometry, minimum end distance and width of the specimen to induce the bearing fracture mode of the bolted joint were determined. And finally, the effect of the outer diameter of washer and clamping pressure on joint strength was also investigated. Since joint strength is dependent on the local glass-fiber weight fraction, experimentally measured strength was modified, considering its irregular values of the specimen molded under various processing conditions in order to obtain a reasonable correlation between the two.

• Journal of Environmental Science International
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• v.33 no.6
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• pp.383-402
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• 2024

Variations in lettuce growth and quality were observed depending on the type of artificial light source. The RGB LED treatment resulted in thick leaf development, leading to higher fresh weight, dry weight, and relative growth rates. Two cultivars, "Tomalin" and "seonpunggold," exhibited increased anthocyanin content and dark red leaf color under conditions of RGB LED treatment. Additionally, they exhibited high chlorophyll content under conditions of RGB LED and RGBFR LED treatments. Particularly, under Red LED treatment, the plants showed elongated leaves with narrow widths, resulting in a higher leaf shape index and a tendency towards leaf curling. Therefore, RGB LED lighting which appropriately blends red, blue, and green lights, is more effective than single lighr sources at improving lettuce growth and quality.

• Journal of Microbiology and Biotechnology
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• v.9 no.2
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• pp.140-146
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• 1999

When methanol was the sole carbon source, Methylobacterium organophilum NCIB 11278, a facultative methylotroph, accumulated Poly-$\beta$-hydroxybutyrate (PHB) as 59% (w/w) of dry cell weight under potassium limitation, 37% under sulfate limitation, and 33% under nitrogen limitation. Based on a stoichiometric analysis of PHB synthesis from methanol, it was suspected that PHB synthesis is accompanied by the overproduction of energy, either 6-10 ATP and 1 $FADH_2$ or 6 ATP and 3 NADPH to balance the NADH requirement, per PHB monomer. This was confirmed by observation of increased intracellular ATP levels during PHB accumulation. The intracellular ATP with limited potassium, sulfate, and ammonium increased to 0.185, 0.452, and 0.390 $\mu$moles ATP/g Xr (residual cell mass) during PHB accumulation, respectively. The intracellular ATP level under potassium limitation was similar to that when there was no nutrient limitation and no PHB accumulation, 0.152- 0.186 $\mu$moles ATP/g Xr. We propose that the maximum PHB accumulation observed when potassium was limited is a result of the energy balance during PHB accumulation. Microorganisms have high energy requirements under potassium limitation. Enhanced PHB accumulation, in ammonium and sulfate limited conditions with the addition of 2,4-dinitrophenol, which dissipates surplus energy, proves this assumption. With the addition of 1 mM of 2,4-dinitrophenol, the PHB content increased from 32.4% to 58.5% of dry cell weight when nitrogen limited and from 15.1 % to 31.0% of dry cell weight when sulfate limited.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.6
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• pp.914-922
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• 1999

An experiment was conducted to determine the effects of dietary energy and protein on performance and carcass composition of broilers under heat stress during the growing period (3-6 weeks). A factorial arrangement of three levels of energy (3.0, 3.2 and 3.4 kcal/g), three levels of protein (18, 20 and 22%), an two rearing temperatures were used in this study. Groups of birds were kept under moderate temperature ($24{\pm}1^{\circ}C/24h$) or hot cycling temperature ($26-34^{\circ}C/6h$, $34{\pm}1^{\circ}C/12h$, and $34-26^{\circ}C/6h$). Body weight (BW), weight gain (WG), feed intake (FI), feed conversion (feed : gain) (FC), carcass weight (CW), carcass yield (YP), breast meat (BM), abdominal fat (AF), drumsticks (DS), and thighs (TH) percentages were determined at the end of the experiment. Hot cycling temperature significantly (p<0.05) decreased BW, WG, FI, CW, and BM, increased FC, YP, DS, and TH but did not affect AF. High energy significantly (p<0.05) increased BW, WG, CW, YP, AF, and TH, decreased FI and FC but did not affect BM or DS. This improvement was observed only under moderate temperature resulting in significant (p<0.05) energy by temperature interaction. High protein significantly (p<0.05) increased BW, WG, CW and BM, decreased AF but did not affect FI, FC, TP, DS, or TH. There were no significant protein by temperature interactions for any of the parameters tested except CW. It is concluded, under the conditions imposed in this experiment, that increasing dietary energy did not alleviate the depressing effect of heat stress while increasing dietary protein up to 22% improved the performance of broilers irrespective of rearing temperature.

• Korean Journal of Weed Science
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• v.15 no.4
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• pp.270-277
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• 1995

The research was carried out in tray in greenhouse equipped with Auto Foggy Systems(SAE KI RIN Co.) and the results were summarized as follows. 1. The number of leaves and fresh weight of Chinese cabbage were not different, but the plant height was increased due to foggy condition. 2. Plant height was decreased in 40 and 60 days of weed competition by foggy condition, the number of leaves was not different. And, shoot fresh weight was decreased by in all the duration of weed competition under foggy condition. Especially, that was greater in 40 days. 3. The shoot fresh weight was decreased by pendimethaline and napropamide application under foggy condition. Also, the number of leaves was decreased at 60 days after transplanting by pendimethaline application under foggy condition. 4. Pendimethalin, napropamide and alachlor treatments were not different in the weeding efficacy between non-foggy and foggy conditions. Trifluraline showed lower weeding efficacy by seedling emergence of large crabgrass as the days of treatment got longer under foggy condition.

• Journal of Chest Surgery
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• v.29 no.11
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• pp.1191-1196
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• 1996

Recently, if there is pulmonary hypertension in ventricular septal defect, early operation without pulmonary artery banding is recommended even though patient is under 1 year in age or has low body weight. We also had been performing operations under the above mentioned policy. From October 1986 to December 1995, eighty eight cases of ventricular septal defect under 10Kg in body weight were operated upon by open cardiac surgery. Mortality was compared by age, body weight and degree of pulmonary vascular hypertension. Total mortality was 10cases(11.4%); under 6months, the mortality was 5 in 14 cases(35.8%), from 6 months to 1year, 4 in 43 cases(9.3%) and over 1 year, one in 31 cases(3.2%), while there was no significant difference in mortality compared by degree of pulmonary vascular hypertension. And mortality under 6months in age was so high regardless of severity of pulmonary hypertension. After this exprience, too early operation,. especially under 6 months, should be considered very prudently, unless there were life threatning heart failure, impending irreversible pulmonary vascular bed change or failure to thrive.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2001.04a
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• pp.74-78
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• 2001

In this study, a multi-step optimization technique combined with a simple genetic algorithm is introduce to the structural design optimization of a high speed machining center. In this case, the design problem is to find out the best design variables which minimize the static compliance, the dynamic compliance, and the weight of the machine structure and meet some design constraints simultaneously. Dimensional thicknesses of the thirteen structural members along the static force loop of the machine structure are adopted as design variables. The first optimization step is a static design optimization, in which the static compliance and the weight are minimized under some dimensional and safety constraints. The second step is a dynamic design optimization, where the dynamic compliance and the weight are minimized under the same constraints. After optimization, the weight of the moving body was reduced to 9.1% of the initial design respectively. Both static and dynamic compliances of the optimum design are also in the feasible range even thought they were slightly increased than before.

• Journal of Environmental Science International
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• v.13 no.6
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• pp.581-589
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• 2004

This paper describes the amount of food consumption and the change of total weight of abalone under a vibration with noise that can be occurred due to piling work. This experiment was conducted in the aquarium in Yosu National University. In normal situation the juvenile stage shell's(total length is 1～1.5cm) amount of food consumption was 0.81g, the middle stage shell(total length is 3～3.5cm) was 13.61g, and the adult stage shell (Total length is 7～7.5cm) was 43.l9g per 5 organisms in 24 hours, while the experimental group was observed low numerical value compared normal groups. The abalones' food consumption and total weight in both groups, the intermittent and continuance impact with noise and vibration, was reduced during this experiment. The abalones' food consumption and total weight in the experimental groups without vibration were recorded slightly high numerical value than the experimental groups with noise and vibration. Based on this experimental data we could conjecture the noise and vibration are harmful factors to bring up a physiological stress to abalones. Especially, the vibration impact by piling works could produce a considerably unfavorable effect to the abalones than noise impact.