• Korean Journal of Oriental Medicine
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• v.13 no.2 s.20
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• pp.149-155
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• 2007

Objective : Using a spontaneously hypertensive rat (SHR) model of essential hypertension, this study investigated whether electroacupuncture (EA) could reduce early stage hypertension by examining whether EA increased nitric oxide (NO) levels in plasma, which compensates for elevated blood pressure (BP). Methods : EA was applied to the acupoint, Baekhoe (GV20), and to a non acupoint in the tail at 10 Hz and an intensity of 1 mA for 10 minutes on the first and fourth day of the week for three weeks under isoflurane anesthesia. In conscious SHR and normotensive Wistar Kyoto (WKY) rats, blood pressure was determined the day after EA treatment by the tail cuff method using an automatic BP monitoring system. We also measured NO concentration of blood serum in SHR and WKY. Results : Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were lower after 3 weeks of GV20 treatment than in non EA treated or non acupoint treated SHR rats. The NO level of plasma was significantly lower in hypertensive SHR than in normotensive WKY. EA prevented the augmentation of blood pressure, and also increased NO concentrations from $7.91{\pm}0.42$ ${\mu}M$ to $11.50{\pm}0.93$ ${\mu}M$ in SHR serum. Conclusions : We suggest that acupuncture may be an early intervention to delay the development of hypertension and enhance NO/NOS activity.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.27 no.7
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• pp.477-482
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• 2014

$CaNb_2O_6:RE^{3+}$ (RE=Sm or Eu) phosphor powders were prepared with different contents of activator ions by using solid-state reaction method. The X-ray diffraction patterns exhibited that the phosphors synthesized with different activator ions showed an orthorhombic system with a main (131) diffraction peak. The maximum size of the grain particles, determined from the measurement of scanning electron microscopy, was observed at 0.05 mol of $Eu^{3+}$ ions and at 0.01 mol of $Sm^{3+}$. As for the $Eu^{3+}$-doped phosphor powders, the excitation spectra were composed of a broad band peaked at 278 nm and several weak bands in the range of 350~500 nm, and the highest red emission spectrum was observed at 0.15 mol of $Eu^{3+}$ ions. As for the $Sm^{3+}$-activated phosphor powders, three strong emission bands under excitation at 273 nm were observed at 570, 612, and 659 nm, respectively. The intensities of all the emission bands approached maxima for 0.05 mol of $Sm^{3+}$ ions. The optical properties show that the $Eu^{3+}$- or $Sm^{3+}$-doped $CaNb_2O_6$ powders are promising red-orange emitting phosphor powders applicable to full-color photonic devices.

• International Journal of Highway Engineering
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• v.18 no.5
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• pp.75-82
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• 2016

PURPOSES : This study was initiated to estimate the benefits from the campaign to prevent drowsy driving crashes on expressways. The study was conducted by the Korea Expressway Corporation using a contingent valuation method. METHODS : First, a questionnaire was designed for a preliminary survey. From the survey's results, the initial willingness to pay for the campaign was determined by averaging different amounts of payments chosen under virtual scenarios in the survey. The willingness to pay data was used to find a first bid price for the open-ended method used for the second survey. After that, a primary questionnaire was designed and conducted using a single dichotomous choice question (SDBCQ). Drivers at expressway resting areas were asked their willingness to pay for the campaign. Based on statistical analysis using data collected from the second survey, the mean willingness to pay was estimated using a probability utility function. Finally, the benefit from the campaign was calculated using the estimated willingness to pay and accident data on expressways. CONCLUSIONS : Based on the result from the contingent valuation method, the benefit from the campaign to prevent drowsy driving crashes was estimated to be 170.6 won per expressway trip. The benefit is to be paid as an additional toll. In addition, the traffic crash cost estimate is about 2,209,680,000 won less than the cost during the same period in 2014.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.35 no.5
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• pp.539-545
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• 2011

Combustion characteristics of industrial radiant tube (RT) burners with forced oscillating combustion technology are investigated using a real-scale (125,000 kcal/h) industrial RT burner facility in both laboratory and field tests. Three different types of industrial RT burners using a by-product gas from the iron-and-steelmaking process are examined in a laboratory facility equipped with a W-type RT. During the field tests, an industrial RT burner is characterized in a large facility equipped with multiple RTs. Their performance and emission controls are investigated under diverse operating conditions. The feasibility of the forced oscillating combustion technology is evaluated by the extent of $NO_x$ reduction and the efficiency improvement. These improvements are able to save energy, extend the RT lifetime, and enhance productivity. The operating conditions that achieve the best performance and emission control for each RT burner are determined.

• Korean Journal of Animal Reproduction
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• v.20 no.1
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• pp.43-52
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• 1996

This study was carried out to establish the in vitro short-term culture system of developing male germ cells by purifing germ cells of various stages. The decapulated testicular cells were incubated with collagenase (lmg/ml) and try psin (2.5mg/ml) in HBSS. After separating male germ cell, the separated germ cells were stained with heamatoxylin/eosin and determined developing stages under light microscopy. The purity of pachtene spermatocytes a and round spermatid were 85%, respectively. Yield of total male germ cells was highly variable between individuals, with a mean value of 3.5 to 4.5 ${\times}$ 10$^7$ cells/testis. Viability of the cell was over 97% after separation. In DMEM medium, the optimal cell number for culture is approximately 1 x 10$^5$ cells/dish, but low cell den-sities than 1 ${\times}$ 10$^5$ cell/dish showed a decreased cell viability. Furthermore, about :36.8% of pac-hytene cells was successfully cultured for 6 days and some of cells were developed to secondary spermatids and round spermatids. Therefore, our data suggested that this culture conditions will be utilize as a feasible tools to produce tran-sgenic livestock using techniques such as intrac-ytoplasmic injection and cell fusion.

• The Korean Journal of Physiology and Pharmacology
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• v.1 no.2
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• pp.151-159
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• 1997

In an attempt to investigate whether hemorrhage affects the gene expression of the renin-angioteusin system (RAS) components in the brain and peripheral angiotensin-generating tissues, changes in mRNA levels of the RAS components in response to hemorrhage were measured in conscious unrestrained rats. Wistar rats were bled at a rate of 3 ml/kg/min for 5 min, and then decapitated 7 h after hemorrhage. Levels of mRNA for renin, angiotensinogen and angiotensin $II-AT_1$ receptor subtypes ($AT_{1A}$ and $AT_{1B}$) were determined with the methods of northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR). Hemorrhage produced a profound hypotension with tachycardia, but blood pressure and heart rate recovered close to the basal level at 7 h. Plasma and renal renin levels were significantly increased at 7 h. Hemorrhage induced rapid upregulation of gene expression of both $AT_{1A}$ and $AT_{1B}$ receptor subtypes in the brainstem and hypothalamus, downregulation of them in the adrenal gland and liver. However, renin mRNA level increased in the brainstem, decreased in the liver, but was not changed in the hypothalamus, kidney and adrenals after hemorrhage. Angiotensinogen mRNA level was not significantly changed in any of the tissue except a slight increase in the liver. The kidney and liver did not show any significant change in gene expression of the RAS components. These results suggest that gene expression of the RAS in central and peripheral tissues are, at least in part, under independent control and the local RAS in each organ plays specific physiologic role.

• Journal of the Korean Society of Safety
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• v.33 no.5
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• pp.15-20
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• 2018

This paper describes the fusing time characteristics of Light PVC Sheathed Circular Cord(VCTF) and Tray Frame Retardant(TFR) cables according to increased temperature under over current condition. The experimental equation will be used to determine the validity and reliability of the test results. The over current flowed 3, 5 and 10 times higher than the amount of allowable current using DC power supply with DAQ(Data Acquisition) measurement system. An infrared radiation heater, which was controlled by a variable AC auto transformer, was used to increase the temperature from room temperature to 50, 100 and 150 degrees Celsius. First, two type of cables were analyzed those with different cross-sectional areas with in the same structure and those with different structures with in the same cross-sectional areas. Then, it was determined how fusing time had been influenced according to the cross-sectional areas and different structures, respectively. The cable resistance was increased by joule heating according to increasing temperature. Therefore, the allowable current of cable is decreased. Finally, the fusing time of the cable was decreased due to increased temperatures at current flow, which were 3 times the amount of allowable current. The instantaneous breakdown was observed when current flow was 5 and 10 times over the amount of allowable current. The fusing time is directly affected by the structure of cable insulation.

• Journal of Microbiology and Biotechnology
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• v.10 no.2
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• pp.251-257
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• 2000

Chitin synthases are identified as key enzymes of chitin biosynthesis in most of the fungi. Among them, chitin synthase II has been reported to be and essential enzyme in chitin biosynthesis, and exists as a membrane-bound form. To search and screen new antifungal agents from natural resources to inhibit chitin synthase II, the assay conditions were established using the enzyme isolated from Saccharomyces cerevisiae ECY38-38A(pAS6) that overproduces only chitin synthase II. This enzyme was activated only by partial proteolysis with trypsin. Its actibity reached the maximum at $80{\;}\mu\textrm{g}/ml$ of trypsin and was strongly stimulated by 2.0 mM $Co^{2+}$, 1.0 nM UDP-[$^{14}C$]-GicNAc, and 32 mM free-GlcNAc. Under these assay conditions, the highest chitin synthase II activity was observed by incubation at $30^{\circ}C$ for 90 min. However, and extremely narrow range of organic solvents up to as much as 25% of DMSO and 25% of MeOH was useful for determining optimal assay conditions. After a search or potent inhibitors of chitin synthase II from natural resources, prodigiosin was isolated from Serratia marcescens and purified by solvent extration and silica gel column chromatographies. The structure of prodigiosin was determined by UV, IR, Mass spectral, and NMR spectral analyses. Its molecular weight and formula were found to be 323 and $C_{20}H_{25}N_{3}O$, respectively. Prodigiosin ingibited chitin synthase II by 50% at the concentration of $115{\;}\mu\textrm{g}/ml$.

• International Journal of Highway Engineering
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• v.13 no.1
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• pp.239-249
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• 2011

The behavior of the fiber reinforced concrete (FRC) base under environmental loads was analyzed numerically as a fundamental study to develop a high structural and functional performance composite pavement system in which the base was formed using FRC and the asphalt or cement concrete surface was placed on it. A two-dimensional finite element model of the FRC base was developed and the sensitivity study was performed with the variables including slab thickness of base, thermal expansion coefficient, elastic modulus, and tensile and compressive strengths. The crack spacing and crack width were selected as representatives of the base behavior. The effects of the selected variables on the crack spacing and crack width were analyzed and the sensitive variables were determined. The results of this study could be useful to determine the optimal material properties of the FRC base for combining well with the surface materials.

• Journal of the Earthquake Engineering Society of Korea
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• v.11 no.1 s.53
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• pp.21-27
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• 2007

In this study, an approach that installs seismic isolation bearings was proposed for the seismic retrofit of the existing bridges. The method that replaces all existing bearings with seismic isolators was proposed already. However, in this study, we recommend to utilize the existing bearings for the benefit of safety and cost. According to our proposal, the seismic isolators do not support vertical loads but they Just function as the period shifter and the horizontal damper. To verify this approach experimentally, the real scale bearings and lead rubber bearings far the real highway bridges were designed and fabricated. And the responses of this isolated bridges to the assumed earthquakes were determined by the pseudo dynamic test scheme. The test results were also compared to the responses computed by the well known structural analysis software to check the reliability of the test. From the test results, we found that the retrofitted bridges using the proposed method showed stable performances under earthquakes.