• Title/Summary/Keyword: under-determined system

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Evaluation of Tissue Culture Efficiency in a Speed Breeding System for Stable and Sustainable Supported Wheat (Triticum aestivum) Immature Embryogenesis (안정적이며 지속적 밀(Triticum aestivum) 미성숙배 조직배양을 위한 스피드 브리딩 조건의 배양 효율 검정)

  • Lee, Geon Hee;Kim, Tae Kyeum;Choi, Chang Hyun;Kim, Jae Yoon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.365-376
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    • 2020
  • Immature embryogenesis is a useful process in wheat tissue culture, including transgenic technology, because of its high regeneration efficiency compared to that in other tissues. However, it is a very labor-intensive and time-restrictive method, because the preparation of immature embryos is limited to the optimal time after flowering. In this experiment, 'Speed Breeding', a breeding technique that accelerates breeding generation advancement by extending the photoperiod, was applied to the wheat variety 'Bobwhite'. A controlled growth room was constructed by adjusting the photoperiod (22-hour light/2-hour dark) using LED lights at temperature of 22℃. After vernalization of the Bobwhite seeds at 4℃ for 4 weeks, the seedlings were grown in a controlled growth room and a greenhouse to compare the heading date. In both conditions, calli were induced from immature embryos on the 11th day after flowering. After 4 weeks, the calli were transferred to a regeneration medium. Regeneration efficiencies under greenhouse conditions and Speed Breeding conditions were determined as 45.05% and 43.18%, respectively. Antioxidant enzyme activity and reference gene expression analysis were performed to confirm the presence of stress due to an extremely long-day photoperiod. As a result, the antioxidant enzyme activity was not distinguished from that of the greenhouse condition. The reference gene expression analysis revealed that the PsaA and CDC genes were highly expressed under the Speed Breeding condition. However, expression of PsbA was similar expression in both conditions. These results will provide useful information for the application of immature embryogenesis to the wheat transformation system.

Characterization of Degradation features and Degradative Products of Poplar Wood(Populus alba${\times}$glandulosa) by Flow Type-Supercritical Water Treatment (초임계수에 의한 현사시 목분의 분해특성 및 분해산물 분석)

  • Choi Joon-Weon;Lim Hyun-Jin;Han Kyu Sung;Kang Ha-Young;Choi Don-Ha
    • Journal of Korea Foresty Energy
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    • v.24 no.1
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    • pp.39-46
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    • 2005
  • In this study, the possibility of sugar conversion of poplar wood(Populus $alba{\times}rglandulosa$) and their degradation features of major wood components were characterized using flow type supercritical water treatment system. The finely ground poplar wood meals were treated for 2min. under subcritical condition$(23MPa,\;275^{\circ}C\;and\;325^{\circ}C)$ and supercritical condition $(23MPa,\;375^{\circ}C\;and\;415^{\circ}C)$. respectively. The degradation products of poplar wood meals appeared brownish colors, including undegraded solids. Increasing the temperature of the system, the degradation rate of poplar wood meals was accelerated and reached up to $94\%\;at\;375^{\circ}C$. The total amount of reducing sugars in degradation products determined by DNS method were gradually lowered when the temperature condition became severe. This indicated that the reducing sugars formed were further degraded to kan derivatives by certain side reaction such as pyrolysis under higher temperature. In order to characterize degradation features of lignin, the degradation products were extracted with ethylacetate and the organic phases were subjected to GC-MS analysis. Main lignin degradation products were identified to vanillin, guaiacol, syrinaldehyde, 4-prophenyl syringol and dihydrosinapyl alcohol, which could be formed by the cleavage of ether linkages in lignin polymers by high temperature condition.

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A Study on Nonstoichiometry and Physical Properties of the Mixed Valency $Sr_{1+x}Dy_{1-x}FeO_{4-y}$Ferrite System (혼합원자가 $Sr_{1+x}Dy_{1-x}FeO_{4-y}$훼라이트계의 비화학양론과 물성 연구)

  • Chul Hyun Yo;Eun Seok Lee;Woong Bum Pyon;Moo Sil Pyon
    • Journal of the Korean Chemical Society
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    • v.32 no.1
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    • pp.3-8
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    • 1988
  • Nonstoichiometric solid solutions of the $Sr_{1+x}Dy_{1-x}FeO_{4-y}$ system (x = 0. 00, 0. 25, 0. 50, 0. 75 and 1. 00) with layered $K-2NiF_4$ type structure were prepared at 1200$^{\circ}$C under atmospheric pressure. X-ray powder diffraction spectra show that the crystallographic phases of the samples are tetragonal within the x range. Nonstoichiometric chemical formulas have been determined by Mohr salt analysis and it shows that the amount of $Fe^{4+}$ ion or ${\tau}$ value increases with increasing x. Electrical conductivities of the samples which were measured in the temperature range of $-100{\sim}200^{\circ}$C under atmospheric air pressure are varied within the semiconductivity range of $l0^{-8}{\sim}10^{-2}(ohm^{-1}{\cdot}cm^{-1}$) and the activation energies are also varied from 0.02 to 0.08 eV. Mixed valency state of $Fe^{3+}$ and $Fe^{4+}$ in the sample of $Sr_{1.00}Dy_{1.00}FeO_{4.04}$ was identified again by Mossbauer spectrum at 200K.

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Estimation and validation of the genetic coefficient of cv. Superior for the DSSAT-CSM (DSSAT 작물모형을 위한 수미품종의 품종모수의 결정과 기후변화에서의 활용)

  • Bak, Gyeryeong;Lee, Gyejun;Lee, Eunkyeong
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.20 no.2
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    • pp.166-174
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    • 2018
  • Potato(Solanum tuberosum L.) is one of the major food crop in the world following rice, wheat, and maize. It is thus important to project yield predict of potato under climate change conditions for assessment of food security. A crop growth modelling is widely used to simulate crop growth condition and total yield of various crops under a given climate condition. The decision support system for agrotechnology transfer (DSSAT) cropping system model, which was developed by U.S. which package integrating several models of 27 different crops, have been used to project crop yield for the impact assessment of climate change on crop production. In this study, we simulated potato yield using RCP 8.5 climate change scenario data, as inputs to the DSSAT model in five regions of Korea. The genetic coefficients of potato cultivar for 'superior', which is one of the most widely cultivated potato variety in Korea were determined. The GenCalc program, which is a submodule of the DSSAT package, was used to determine the genetic coefficients for the superior cultivar. The values of genetic coefficients were validated using results of 39 experiments performed over seven years in five regions. As a case study, the potato yield was projected that total yields of potato across five regions would increase by 26% in 2050s but decrease by 17% in 2090s, compared with 2010s. These results suggested that the needs for cultivation and irrigation technologies would be considerably large for planning and implementation of climate change adaptation for potato production in Korea.

An Experimental Study for the Design of Household-Storage Facilities (부엌 수납장 설계 기준 설정을 위한 기초적 연구)

  • 최재순
    • Journal of the Korean Home Economics Association
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    • v.20 no.4
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    • pp.155-168
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    • 1982
  • What is attempted here is to find out an optimum method for the design of physical environments that could save human energy expenditures and safely perform household duties. There are, if any, very little amount of research done in this area. This is particulary so when the work relates to the designing of household storage facilities in the light of the energy metabolism of human body. The first step to this study, therefore, is to find out the ways by which we can determine the energy metabolism of human body accurately. To measure the volume and the concentration of human respiration continuously and automatically, a new apparatus is selected here. This includes the recording system with the Wright Respirometer and the Expired Gas Analyzer as well as the computer system to multiply volume by concentration of human respiration and to integrate them for a given time. Then, the author experimented on the reliability of this apparatus and came to the conclusion that this apparatus satisfied our need to research the energy metabolism of human body. Next, the general plan and procedures to experiment with this apparatus have been determined as follows: 1) subjects are three young and sound females. Their physical characteristics are shown in Table 1 and most approximates the standard characteristics of Japanese females, 2) height of open shelves is selected in such away as to correspond to the respective height of each subject(see Table 2), 3) utensils to be stored are box shaped object, which weight is 0.5kg, 1.0kg, 2.0kg or 3.0kg, 4) working motions are given while one or two hands as to place utensil on each shelf from the standard working board, 85 cm in height and then to place back it on the board from the shelf and repeated in constant velocity as 10 times per a minute, 5) each posture of motion is chosen by each subject in free, 6) procedures of measurement of human energy metabolism ard shown at(6), 1, Section 3 as specific methods for using this apparatus. Findings of this study are as follows: 1. Human energy expenditures for storing utensils on shelves by each subject are shown in quantity more accurately than in any other studies, under varying conditions about height of shelves, load of utensils and working motion with one hand and two hands (see Fig. 8~13 and Table 3). 2. Experimental formulae of human energy expenditure for that work are shown as formula (8), (9) and (10), to generalize results of 1. and to apply those for working motion under given conditions. 3. As results of analysis on gained data, we are able to show the standard model of human energy expenditures for storing untensils on shelves by the standard Japanese female (see Table 7 and Fig. 14).

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Effect of Electroacupuncture at SP-6 with Different Durations on Minimum Alveolar Concentration and the Cardiovascular System under Isoflurane Anesthesia in Dogs (개에서 Isoflurane 마취시 SP-6 혈위의 전침자극시간이 최소폐포농도 및 심맥관계에 미치는 영향)

  • Jeong, Seong-Mok
    • Journal of Veterinary Clinics
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    • v.19 no.3
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    • pp.283-289
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    • 2002
  • The effects of electroacupuncture (EA) at SP-6 with different durations on the minimum alveolar concentration (MAC) and on the cardiovascular system were evaluated in dogs under isoflurane anesthesia. Eight healthy male beagles were randomly assigned to four study groups (n = 5/group) with washout period of 7 days for recovery and anesthetic withdrawal between experiments. Four study groups were control, nonacupoint electrical stimulation (NA), EA for 30 minutes (SP-6) and continuous EA for 70 or 90 minutes (SP-6C). For the nonacupoint electrical stimulation group, needles were inserted into the nonacupoint at the muscle bellies of left triceps brachii and right quadriceps femoris. MAC and cardiovascular parameters were determined after EA at SP-6 acupoint and at nonacupoint. Thirty minutes of EA and continuous EA until re-determination of MAC at SP-6 acupoint lowered the MAC of isoflurane by 21.3$\pm$8.0% and 16.1$\pm$4.6%, respectively (p<0.05). The decrements in MAC values were not significantly different between two EA groups. However, electrical stimulation of nonacupoint did not induce a significant change in MAC. In SP-6 and SP-6C groups, significant changes in cardiovascular parameters were not observed. These results indicate that EA at SP-6 have an advantage in isoflurane anesthesia in terms of reducing the requirement for anesthetics and minimizing cardiovascular side effects. EA for 30 minutes at maximum might be the sufficient time to produce acupuncture analgesia.

The Relationship of Froude Number and Developed Cloud Band Locations Near Yeongdong Region Under the Siberian High Pressure System (시베리아 고기압 영향으로 영동지역 부근에서 발달한 구름대의 위치와 Froude 수와의 관계)

  • Kim, Yu-Jin;Kim, Man-Ki;Lee, Jae Gyoo
    • Atmosphere
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    • v.29 no.3
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    • pp.325-342
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    • 2019
  • Precipitation and no-precipitation events under the influence of the Siberian high pressure system in Yeondong region, were analysed and classified as four types [obvious precipitation event (OP) type, obvious no-precipitation event (ON) type, ambiguous precipitation event (AP) type and ambiguous no-precipitation event (AN) type], according to the easiness in determining whether to have precipitation or not in Yeongdong region, to help in improving the forecast skill. Concerning the synoptic pressure pattern, for OP type, the ridge of Siberian high extends from Lake Baikal toward Northeast China, and there is a northerly wind upstream of the northern mountain complex (located near the Korean-Chinese border). On the other hand, for ON type, the ridge of Siberian high extends southeastward from Lake Baikal, and there is a westerly wind upstream of the northern mountain complex. The pressure pattern of AP type was similar to the OP type and that of AN type was also similar to ON type. Thus it was difficult to differentiate AP type and OP type and AN type and ON type based on the synoptic pressure pattern only. The four types were determined by U (wind speed normal to the Taebaek mountains) and Froude number (FN). That is, for OP type, average FN and U at Yeongdong coast are ~2.0 and ${\sim}6m\;s^{-1}$, and those at Yeongseo region are 0.0 and $0.1m\;s^{-1}$, respectively. On the contrary, for ON type, average FN and U at Yeongdong coast are 0.0 and $0.2m\;s^{-1}$, and those at Yeongseo region are ~1.0 and ${\sim}4m\;s^{-1}$, respectively. For AP type, average FN and U at Yeongdong coast are ~1.0 and ${\sim}4m\;s^{-1}$, and those at Yeongseo region are 0.0 and $0.2m\;s^{-1}$, whereas for AN type, average FN and U at Yeongdong coast are 0.1 and $0.6m\;s^{-1}$ and those at Yeongseo region are ~1.0 and ${\sim}3m\;s^{-1}$, respectively. Based on the result, a schematic diagram for each type was suggested.

Development of nutrigenomic based precision management model for Hanwoo steers

  • Chandima Gajaweera;Dong Hun Kang;Doo Ho Lee;Yeong-Kuk Kim;Bo Hye Park;Sun Sik Chang;Ui Hyung Kim;Seung Hwan Lee;Ki Yong Chung
    • Journal of Animal Science and Technology
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    • v.65 no.3
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    • pp.596-610
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    • 2023
  • Focusing high marble deposition, Hanwoo feedlot system uses high-energy diet over the prolonged fattening period. However, due to the individual genetic variation, around 40% of them are graded into inferior quality grades (QG), despite they utilized the same resources. Therefore, focusing on development of a nutrigenomic based precision management model, this study was to evaluated the response to the divergent selection on genetic merit for marbling score (MS), under different dietary total digestible nutrient (TDN) levels. Total of 111 calves were genotyped and initially grouped according to estimated breeding value (high and low) for marbling score (MS-EBV). Subsequently, managed under two levels of feed TDN%, over the calf period, early, middle, and final fattening periods following 2 × 2 factorial arrangement. Carcasses were evaluated for MS, Back fat thickness (BFT) and Korean beef quality grading standard. As the direct response to the selection was significant, the results confirmed the importance of initial genetic grouping of Hanwoo steers for MS-EBV. However, dietary TDN level did not show an effect (p > 0.05) on the MS. Furthermore, no genetic-by-nutrition interaction for MS (p > 0.05) was also observed. The present results showed no correlation response on BFT (p > 0.05), which indicates that the selection based on MS-EBV can be used to enhance the MS without undesirable effect on BFT. Ultimate turnover of the Hanwoo feedlot operation is primarily determined by the QGs. The present model shows that the initial grouping for MS-EBV increased the proportion of carcasses graded for higher QGs (QG1++ and QG1+) by approximately 20%. Moreover, there appear to be a potential to increase the proportion of QG 1++ animals among the high-genetic group by further increasing the dietary energy content. Overall, this precision management strategy suggests the importance of adopting an MS based initial genetic grouping system for Hanwoo steers with a subsequent divergent management based on dietary energy level.

Field Evaluation of Traffic Wandering Effect on Asphalt Pavement Responses (차량의 횡방향 주행이격에 의한 아스팔트 콘크리트 포장의 응답특성 분석)

  • Seo, Youngguk;Kwon, Soon-Min;Lee, Jae-Hoon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.3D
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    • pp.453-459
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    • 2006
  • This paper presents an experimental evaluation of wandering effect on asphalt concrete pavement responses. A laser-based wandering system has been developed and its performance is verified under various field conditions. The portable wandering system composed of two laser sensors with Position Sensitive Devices can allow one to measure the distance between laser sensors and tire edges of moving vehicle. Therefore, lateral position of each wheel on the pavement can be determined in a real time manner. Pavement responses due to different loading paths are investigated using a roll over test which is carried out on one of asphalt surfaced pavements in the Korea Highway Corporation test road. The pavement section (A5) consists of 5 cm thick surface course; 7 cm intermediate course; and 18 mm base course, and is heavily instrumented with strain gauges, vertical soil pressure cells and thermo-couples. From the center of wheel paths, seven equally-spaced lateral loading paths are carefully selected over an 140 cm wandering zone. Test results show that lateral horizontal strains in both surface and intermediate courses are mostly compressive right under the loading path and tensile strains start to develop as the loading offset becomes 40 cm from the wheel path. The development of the vertical stresses in the top layers of subbase and anti-frost is found to be minimal once the loading offset becomes 50 cm.

A Study on the Effect of Acupuncture on Anesthesia and the Mode of Action (The Second Report) (자침(刺鍼)이 마취(痲醉)에 미치는 작용기전(作用機轉) 연구(硏究) (제(第) 2 보(報)))

  • Park, Hee-soo;Park, Kyoung-sik
    • Journal of Acupuncture Research
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    • v.19 no.4
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    • pp.140-151
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    • 2002
  • This study was carried to identify whether acupuncture at several meridian points can affect the human anesthesia or not through the analysis of serum intermediates, such as, melatonin, serotonin, TSH, cortisol in human serum. We investigated the effect of accupuncture on the change of serum intermediates which have frequently been made a subject of discussion owing to its hyponic and sedative properties in practice. The result obtained were as follow ; 1. Serum melatonin levels was hardly affected at 40 minutes after general acupuncture on Hab-Gog, Nae-Gwan, and the other meridian points as compared with that($5.64{\pm}1.02pg/ml$) of resting control group. But on day 1 after acupuncture, these tend to increase significantly ($7.95{\pm}2.05pg/ml$ to $8.21{\pm}1.57pg/ml$, p <0.05). On the other hand, control group under sleep showed the serum level of $7.39{\pm}1.03pg/ml$. Additional acupuncture at In-Dang induced the increased melatonin level, especially at 40minutes after acupuncture(p <0.05). 2. Serotonin level in resting control serum recorded $51.14{\pm}8.17{\mu}g/ml$. Acupuncture at determined meridian point intend to increase average level of serotonin, but not significant. However observation on day 1 after acupuncture at Hab-Gwan meridian point and under sleeping showed the significantly increased serotonin level, $74.05{\pm}35.83{\mu}g/ml$, $70.44{\pm}13.08{\mu}g/ml$, respectively. 3. TSH level in intact human serum recorded $0.85{\pm}0.24{\mu}IU/ml$. and the serum exposed to acupuncture showed the irregular pattern of TSH level in a mean range of $0.76{\pm}0.44{\mu}IU/ml$ to $1.06{\pm}0.38{\mu}IU/ml$, regardless of meridian point or time after acupuncture. 4. The values of serum cortisol in control group were $11.76{\pm}2.06{\mu}g/dl$ in resting, $7.51{\pm}2.85{\mu}/dl$ under sleep, respectively. The level of serum cortisol in 40minutes after acupuncture was markedly (p <0.05) reduced to the serum levels of $5.65{\pm}2.58{\mu}g/dl$ (Hab-Gog, Nae-Gwan), $7.58{\pm}3.21{\mu}g/dl$ (Gyo-Gam, Sin-Mun besides Hab-Gog, Nae-Gwan), $6.43{\pm}3.54{\mu}g/dl$ (In-Dang besides to Hab-Gog, Nae-Gwan), respectively, as compared with control, intending to increase a little on day 1 after acupuncture. From the above results, the analysis of serum intermediates suggest that acupunctuation at meridian points applied to at this study act upon the phase of light anaesthesia or hypnosis, at the same time affect pituitary-adrenal axis rather than hypothalamus-pituitary axis in the secretary system of hormone and also don't affect dorsal-raphe nucleus according to the observation of change transition in serum intermediates, such as TSH, serotonin, and cortisol.

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