• 대한간호학회지
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• 제5권2호
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• pp.92-103
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• 1975

This study was conducted to find out status of delivery environments, delivery attendants, and various immunization practices towards 513 babies who were born during the period from January 1, 1972 to December 31, 1974, in Dongnae Area, Shindong Myon, Chunseong Gun, Ganwon Do. The results and findings obtained from the study are summarized as follows : 1. Of all deliveries, 10.7% of the babies were reported born at the hospitals. 2. Deliveries attended by qualified professionals such as, doctors, midwives or public health nurses, were 22.2% of the total deliveries. Those who delivered alone without having anyone's assistants were as much as 9.7%, The percentage of the deliveries attended by the professional groups were increased year by year among the younger group mothers with the babies birth order being first or second place. 3. Sewing scissors were most frequently used as a tool for cutting the umbilical cord indicating 87.5% in this area. Sterilized tools were used among 50.3% of the women. 4. The usage af vinyl sheet and cement bag paper were frequently used as a delivery sheet, indicating 32.3%. Delivery set which was distributed by the health center was used 18.0%. (excluded the deliveries attended by professional attendants) 5. Immunization practices for the infant were observed as relatively high rate: 92.8% of the infant received D. p. T., 81, 3u/o received against poliomyelitis, 52.8% for small pox, 78.9% with B. C. G., and 18.5%against measles. The percentage af babies complected 3 times shot for D. P. T. and 2 times shot for poliomyelitis vaccine turned out be to 67.8% and 87.5%, respectively.

• Clinical and Experimental Pediatrics
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• 제46권2호
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• pp.192-194
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• 2003

가정분만으로 태어난 후 생후 6일된 신생아에서 전신성 강직-간대 경련이 발생하여 내원한 환아에서, 병력과 진찰 소견상 신생아 파상풍으로 진단하여 근이완제 및 인공환기요법으로 치료한 1례를 경험하였기에 보고하는 바이다.

• 한국식품영양학회지
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• 제22권2호
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• pp.313-319
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• 2009

To improve the growth of human mesenchymal stem cells(hMSCs) under general cell culture conditions(20% $O_2$ and 5% $CO_2$), we examined the effect of s-allylcysteine(SAC), which is known as an antioxidant and the main component of aged-garlic extract, on hydrogen peroxide-induced cellular stress in hMSCs. We found that SAC blocked hydrogen peroxideinduced cell death and cellular apoptosis, but that SAC did not improve the growth of hMSCs during short-term culture. To evaluate the protective effect of SAC, we examined the endogenous expression of the antioxidant enzymes catalase (CAT), superoxide dismutase(SOD), and glutathione peroxidase(Gpx) in hMSCs. Hydrogen peroxide was found to downregulate the expression of CAT, SOD, and Gpx at the protein level. However, in the pre-treatment group of SAC, SAC inhibited the hydrogen peroxide-induced down-regulation of CAT, SOD, and Gpx. Unfortunately, treatment with SAC alone did not induce the up-regulation of antioxidant enzymes and the cell proliferation of hMSCs. Surprisingly, SAC improved cell growth in a single cell level culture of hMSCs. These results indicate that SAC may be involved in the preservation of the self-renewal capacity of hMSCs. Taken together, SAC improves the proliferation of hMSCs via inhibition of oxidative-stress-induced cell apoptosis through regulation of antioxidant enzymes. In conclusion, SAC may be an indispensable component in an in vitro culture system of human MSCs for maintaining self-renewal and multipotent characterization of human MSCs.

• Journal of Microbiology and Biotechnology
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• 제12권3호
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• pp.483-489
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• 2002

The role of $G{\alpha}12\;and\;G{\alpha}13$ in modulating the IgE receptor-mediated histamine secretion in the streptolysin-o-permeabilized human cultured mast cell was investigated. The expression of $G{\alpha}12\;and\;G{\alpha}13$ proteins were regulated during human cultured mast cell differentiation, and a significant correlation was observed between the levels of expression of $G{\alpha}12\;and\;G{\alpha}13$ proteins and IgE receptor-mediated histamine secretion capability in human cultured mast cells. Antibodies against $G{\alpha}12\;and\;G{\alpha}13$ effectively inhibited the IgE receptor-induced histamine release, and the concentration of anti-$G{\alpha}12$ antibody used to inhibit histamine secretion was shown to also inhibit the IgE receptor-mediated elevation of intracellular $Ca^2+$. Therefore, the results suggest that $G{\alpha}12\;and\;G{\alpha}13$ play roles in modulating IgE receptor-activated $Ca^2+$ influx, thereby regulating histamine release in cultured human mast cells. This is the first report to show that $G{\alpha}12\;and\;G{\alpha}13$ are involved in the regulation of $Ca^2+$ mediated exocytosis in human cultured mast cells.

• Asian-Australasian Journal of Animal Sciences
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• 제31권2호
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• pp.237-244
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• 2018

Objective: Piglet pre-weaning mortality is an important variable indicating the efficacy of farrowing management and animal well-being during lactation. The present study determined the association of newborn traits measured soon after birth with piglet pre-weaning mortality and growth. Methods: In total, 805 piglets born from 57 multiparous sows were investigated. Their blood oxygen saturation, blood glucose and rectal temperature at 24 h after birth (RT24h) were monitored. Birth order, sex, skin color, integrity of the umbilical cord, attempts to stand and birth intervention were monitored. Piglets were weighed at day 0, 7, and 21 to evaluate average daily gain (ADG). Results: Piglet pre-weaning mortality for lactation period was 12.6% and cumulative mortality during the first 7 days of age was 8.6%. A higher proportion of piglets with pale skin color died compared to piglets with normal skin color (26.7% vs 7.7%, p<0.001). A higher (p<0.001) proportion of piglets that attempted to stand after 5 min (38.5%) died compared to piglets that attempted to stand within 1 min (6.3%) after birth. Piglet body weight at birth ($BW_B$), blood glucose and the number of piglets born alive (BA) were correlated with ADG (p<0.05). Piglets with $BW_B$ <1.30 kg had higher (p<0.001) mortality rate than piglets with $BW_B{\geq}1.80 kg$ (19.0% vs 3.3%) and piglets with $BW_B$ 1.30 to 1.79 kg (4.0%). Piglet with RT24h <$37.0^{\circ}C$ had higher (p<0.001) mortality rate (86.2%) than piglets with RT24h >$38.5^{\circ}C$ (3.9%). Conclusion: Low $BW_B$ and low RT24h compromise piglet survival during the lactation period in the tropical conditions. Piglets in the litters with a high BA, low $BW_B$ and low blood glucose have reduced ADG.

• 부모자녀건강학회지
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• 제6권1호
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• pp.31-45
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• 2003

This study is designed to produce basic data on the nursing intervention between infants and mothers after discharge from hospitals. This research is carried by investigating the nursing intervention during their after-delivery staying in the hospitals located in Pusan. The object of this study includes the neonatal wards of 51 hospitals. Data collection was carried from the 3rd of September, 2001 to the 4th of October in the same year. Tools for measurement using in this research is the nursing checklist designed by these researchers. The data were analyzed by using SPSS and the output was produced in frequency and percentage considering general characteristics and nursing activities. On the course of investigation, it was revealed that the average number of items checked are as follows; total beds in one hospital came up to 360.1, the beds in each newborn infant ward came up to 36.1, in each ward the numbers of incubators came up to 9.2 and intensive care unit to 1.7. In each ward 8.4 nurses and 2.9 nurse aids were working at that time. In each hospital, secure of air way, physical examination, eye care, umbilical cord care, maintenance of body temperature, identification of infant, nutritive condition, safety and danger management, infection management, mother-infantile interaction, medical assistant to doctor, medication and discharge education were comparatively well done. But, in case of emotional, social and cognitive aspect of nursing activities through interactions between newborn infants and nurses as substitutes are not reached to the adequate level. In the end, this study hereby suggests that further investigation on the device to facilitate the interaction between newborn infant and nurse as a nursing intervention of high quality.

• Molecules and Cells
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• 제40권6호
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• pp.434-439
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• 2017

Gestational diabetes mellitus (GDM), one of the common metabolic disorders of pregnancy, leads to functional alterations in various cells including stem cells as well as some abnormalities in fetal development. Perivascular stem cells (PVCs) have gained more attention in recent years, for the treatment of various diseases. However, the effect of GDM on PVC function has not been investigated. In our study, we isolated PVCs from umbilical cord of normal pregnant women and GDM patients and compared their phenotypes and function. There is no significant difference in phenotypic expression, response to bFGF exposure and adipogenic differentiation capacity between normal (N)-PVCs and GDM-PVCs. However, when compared with N-PVCs, early passage GDM-PVCs displayed decreased initial rates of cell yield and proliferation as well as a reduced ability to promote wound closure. These results suggest that maternal metabolic dysregulation during gestation can alter the function of endogenous multipotent stem cells, which may impact their therapeutic effectiveness.

• 대한소아치과학회지
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• 제36권4호
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• pp.586-590
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• 2009

Leukocyte adhesion deficiency type I(LAD I)은 혈관 내피 세포에 백혈구가 부착하는 과정에 결함이 발생하여 혈관에서 감염부위로의 백혈구의 이주가 방해되어 발생하는 질환으로, 재발성 감염증과 백혈구 증가증을 보이는 희귀 질환이다. 피부와 점막의 괴사성 감염, 장내 패혈증, 제대염, 중이염, 뇌수막염 등의 임상 증상을 보이며, 이러한 환자들의 주요한 구강 내 증상은 심각한 치주 질환과 치조골 소실, 치주낭 형성, 유치열과 영구치열의 부분적 또는 전체적 조기 상실을 보인다. 본 증례는 심한 사춘기전 치주염 소견을 보이는 LAD type I환자로 국소적, 전신적 감염을 예방하기 위해 정기적인 치과 내원으로 치면 세균막 관리를 시행하였다. 감염 시 항생제 투여 및 세균 도말 검사를 시행하였다.

• 한국정밀공학회지
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• 제28권4호
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• pp.521-525
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• 2011

It is known that the low-intensity sound stimulation really affect to grow the cell. The cellular growth mechanism, however, does not been clearly identified even the effect on the low-intensity sound stimulation. The purpose of this study is to investigate the effect of low-intensity sound stimulation on the alveolar UC-MSC proliferation. Before the low-intensity sound stimulation is applied, the UC-MSC are cultured for 24 hours to facilitate their attachments. The cells are divided into two groups. And each was exposed to a medium with or without the low-intensity ultrasound stimulation at 71dB intensity level. The UC-MSC are again divided into three treatment groups of group 1, 2, and 3 and exposed to a frequency at 50Hz, 100Hz, and 1000Hz, respectively. In the results, it is investigated that the growth rates of UC-MSC for the stimulated groups were higher than those of control groups. In 1000Hz frequency, the number of UC-MSC cell is significantly higher than control groups (p>0.05). We would put the hypothesis that the cell growth could be enhanced by an appropriate low-intensity sound stimulation.

• 대한한방소아과학회지
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• 제15권1호
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• pp.235-253
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• 2001

Tae-yeul is the baby's disorder inheritted by its mother during fregnancy. This is due to its mother's overeating hot, fatty food and taking harmful medicine. So internal physical fever is accumulated in mother's body, and it heats up the placenta and the umbilical cord. Generally, this disorder means the skin disease that is occurred from $2{\sim}5$ months to 1 year after birth. It is specialized by the dirty oozing, a severe itching and the rash at its head and face. It is similar in its symptoms such as atopic dermatitis in western medicine. Recently, this disease has the increasing tendency of prevalence rate because of the bad atmosphere and the invironmental pollution. The results were summerised as follows; 1, 2, 3, 4 1. The causes of Tae-yeul are summarized by fragnat woman's contacting a cool air, overating a hot and toxic food and taking emotional shock. 2. The symptoms of Tae-yeul are the edema on upper eyelids, stifling convulsions, flush, the fever of one side body, the asthma of sputum increase, the yawing of the tired mind, the bowl complaint, the red urine and urination disturbance. 3. In the treatment method of Tae-yeul, its mother feed her milk after taking a madicine. So one must expect to be treated slowly. If one tries to treat it with a cool madicine quickly, it can cause vomitting or a serious disorder. 4. In the prescription of Tae-yeul, they used a Moktongsan, a Chiwhnagtang, an Yangyoubang, a Tojuksan, a Whangyunsan, a Juryungtang and a Kamdutang, etc.. For the method of cleaning face or bathing, they used Sasungsan, Whanbaekpijuntang. 5. Tae-yeul including Naesun, Taelyumchang, yousun, etc. is similar to the atopic dermatitis of western medicine in its symptoms.