• 한국식품과학회지
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• 제38권5호
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• pp.684-690
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• 2006

본 연구에서는 비파의 산업적 이용 평가를 목적으로 하여 부위별 비파 추출물의 생리활성 및 감마선 조사효과를 평가하였다. 비파 추출물의 감마선 조사에 의한 색도변화에서 명도 및 적색도는 증가하였고 황색도는 감소하여 더 밝은 색을 띄었다. 총 페놀 함량은 비파 잎에서 94.1 mg/g으로 가장 높았으며, 다음으로 씨, 과육 순으로 나타났으며 조사후의 유의적인 변화는 없었다. 항산화 효과로서는 electron donation ability, ${\beta}-carotene$ blcaching assay, FRAP assay 및 reducing power을 정하였는데 이와 같은 항산화 관련 실험에서 비파 부위별 메탄올 추출물 중 잎이 씨와 과육보다 상대적으로 강한 활성을 보였으며 감마선조사에 의한 유의적인 변화는 관찰되지 않았다(p<0.05). 본 실험에서의 결과로서 비파에서는 총 페놀 함량이 높을수록 항산화 활성 비례 증가함을 시사하는 자료가 얻어졌다. Xanthine oxidase 저해활성 측정결과, 비파 잎이 39.6%, 씨가 35.4%로 유의적 차이는 없었으나 과육은 20.2%로 차이를 나타냈다. 또한, 조사에 의한 유의적 차이는 없었다. 부위별 비파 추출물의 항간효과를 paper disc법을 이용하여 측정한 결과, 잎 추출물이 가장 강한 항균 활성을 가지는 10에서 14mm의 저해환을 나타내었으며, 특히 대장균에 대해서는 감마선 조사에 의한 유의적 변화 없이 가장 강한 항균 활성 나타냈었다. Ames test를 이용한 복귀돌연변이 시험에서는 $10,000\;{\mu}g/plate$이하 일 때 부위별 비파 메탄올 추출물의 돌연변이원성이 나타나지 않는 것으로 확인되었다. 이상의 결과로부터 다량의 페놀성 화합물을 함유하고 있는 비파의 생활 습관성병 예방 및 기능성 천연 소재로서의 개발이 기대되며 감마선 조사에 의한 비파 성분의 화학적 변형에 관한 고찰이 필요하다고 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권4호
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• pp.1407-1412
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• 2012

Background & Objectives: Gastric cancer is a leading cause of cancer-related deaths in both sexes in Iran. This study was designed to assess upper GI endoscopic findings among people > 50 years targeted in a mass screening program in a hot-point region. Methods: Based on the pilot results in Guilan Cancer Registry study (GCRS), one of the high point regions for GC-Lashtenesha- was selected. The target population was called mainly using two methods: in rural regions, by house-house direct referral and in urban areas using public media. Upper GI endoscopy was performed by trained endoscopists. All participants underwent biopsies for rapid urea test (RUT) from the antrum and also further biopsies from five defined points of stomach for detection of precancerous lesions. In cases of visible gross lesions, more diagnostic biopsies were taken and submitted for histopathologic evaluation. Results: Of 1,394 initial participants, finally 1,382 persons (702 women, 680 men) with a mean age of $61.7{\pm}9.0$ years (range: 50-87 years) underwent upper GI endoscopy. H. pylori infection based on the RUT was positive in 66.6%. Gastric adenocarcinoma and squamous cell carcinoma of esophagus were detected in seven (0.5%) and one (0.07%) persons, respectively. A remarkable proportion of studied participants were found to have esophageal hiatal hernia (38.4%). Asymptomatic gastric masses found in 1.1% (15) of cases which were mostly located in antrum (33.3%), cardia (20.0%) and prepyloric area (20.0%). Gastric and duodenal ulcers were found in 5.9% (82) and 6.9% (96) of the screened population. Conclusion: Upper endoscopy screening is an effective technique for early detection of GC especially in high risk populations. Further studies are required to evaluate cost effectiveness, cost benefit and mortality and morbidity of this method among high and moderate risk population before recommending this method for the GC surveillance program at the national level.

• 한방안이비인후피부과학회지
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• 제29권4호
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• pp.14-23
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• 2016

Objectives : One of major complications of diabetes, diabetic ulcer is also one of the main reasons for amputation, and the prevalence rate is 4-10%. Laser therapy is widely used for leg ulcer and diabetic ulcer, and it is known to improve wound epithelialization, cellular content, and collagen deposition. The purpose of this study is to investigate the properties of the laser and the spectrum of laser instruments for diabetic ulcer. Methods : We performed literature search using the PubMed, Cochrane, CINAHL and Web of science for the data in English. In addition, other databases were checked for different languages such as OASIS and NDSL for the literature in Korean, CNKI in Chinese, and CiNii and J-STAGE written in Japanese. We excluded all review article and experimental studies, and only clinical studies using laser or light emitting diode (LED) for diabetic ulcer were selected. Results : A total twenty papers were selected. Different light sources were used as follows: LED, HeNe, InGaAlP, GaAlAs, GaAs, CO2, and KTP. The number of LED studies was 9, and HeNe laser was 7, and InGaAlP and GaAlAs laser was 2, GaAs, CO2, and KTP laser was 1 for each. Various energy density of the clinical study were reported. Conclusions : It is suggested that to select appropriate laser type and give the adequate output power to treat diabetic ulcer. Further evaluation and research for the condition of laser therapy to treat diabetic ulcers are warranted.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제8권2호
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• pp.164-171
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• 2005

목 적: 건강한 만삭아로 출생한 신생아에서 상부위장관 출혈의 임상적 특징, 위내시경 소견, 연관된 위험요소 등을 알아보고자 하였다. 방 법: 2002년 1월부터 2003년 12월까지 을지의과대학교 을지병원 신생아 중환자실에 토혈 혹은 혈변을 주소로 입원하여 위내시경상 출혈성 위염 또는 궤양으로 진단된 9명의 환아들을 대상으로 산전인자, 모체인자, 분만소견, 내시경 소견, 예후 등을 후향적으로 조사하였다. 결 과: 1) 9명의 환아 중 원내 출생아는 5명(5명/3,734명)으로 발생률은 0.13%였고, 모두 적정체중 만삭아로 출생 시 가사소견은 없었다. 2) 산모들의 병력상 산전에 위염, 소화성 궤양, 제산제 복용 등의 기왕력, 위장관 질환의 가족력은 없었고, 분만과정 중 특이소견 없었다. 3) 토혈, 혈변, 반복되는 구토가 주증상이었으며, 출생 후 $4.4{\pm}3.8$일에 발생하였고, 입원 시 활력증후는 양호하였고, 혈액검사소견도 정상이었다. 내시경 검사상 출혈성 위염이 6예, 위궤양이 3예였다. 4) 모든 환아는 $H_2$ 수용체 차단제 치료와 보존적 치료을 실시하였고, 치료 시작 후 $0.9{\pm}0.3$일 이내에 임상증상은 호전되었다. $H_2$ 수용체 차단제는 $18.6{\pm}5.0$일 치료하였으며 추적관찰상 증상이 재발된 환아는 없어서 추적 내시경은 실시하지 않았다. 결 론: 만삭아에서 출혈을 동반한 상부위장관 점막질환은 대부분 예후가 양호하여 임상증상이 호전된 경우에는 추적 위내시경 검사는 불필요한 것으로 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권13호
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• pp.5199-5203
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• 2015

Background: Helicobacter pylori (H.pylori) is associated with chronic gastritis, peptic ulcers, gastric adenocarcinomas and mucosa associated tissue lymphomas. Cytotoxin associated gene A (CagA) is one of the virulence factors of H.pylori. It is hypothesized that reactive oxygen species (ROS) play roles in H.pylori associated disease especially in development of gastric adenocarcinoma. Individuals infected with H.pylori bearing CagA produce more ROS than others. 8-hydroxydeoxyguanosine (8OHdG) is an in vitro marker of DNA damage and oxidative stress. The aim of this study was to investigate the relationship between 8OHdG level, H.pylori infection and CagA and alterations of serum 8OHdG level after H.pylori eradication. Materials and Methods: Patients admitted with dyspeptic complaints and upper gastrointestinal endoscopy were assessed. H.pylori was determined from histopathology of specimens. Serum 8OHdG levels of three groups (H.pylori negative, H. pylori positive CagA negative and H.pylori positive CagA positive) were compared. Patients with H.pylori infection received eradication therapy. Serum 8OHdG levels pretreatment and posttreatment were also compared. Results: In total, 129 patients (M/F, 57/72) were enrolled in the study. Serum 8OHdG level of H.pylori negative, H. pylori positive CagA negative and H.pylori positive CagA positive groups were significantly different ($5.77{\pm}1.35ng/ml$, $5.43{\pm}1.14ng/ml$ and $7.57{\pm}1.25ng/ml$ respectively, p=0.05). Furthermore, eradication therapy reduced serum 8OHdG level ($6.10{\pm}1.54ng/ml$ vs $5.55{\pm}1.23ng/ml$, p=0.05). Conclusions: Individuals infected with H.pylori bearing CagA strains have the highest serum 8OHdG level and eradication therapy decreases the serum 8OHdG level. To the best of our knowledge this is the first study that evaluated the effect of CagA virulence factor on serum 8OHdG level and the effect of eradication therapy on serum 8OHdG levels together. Eradication of CagA bearing H.pylori may prevent gastric adenocarcinoma by decreasing ROS. 8OHdG level may thus be a good marker for prevention from gastric adenocarcinoma.

• 한국버섯학회지
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• 제3권2호
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• pp.71-74
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• 2005

차가는 배양균사로부터 위염, 위궤양 및 여러 가지 암에 효력을 나타내는 것으로 알려진 베풍긴(befungin) 약제의 생산을 위하여 사용되고 있다. 차가는 자작나무 줄기에서 주로 생산되는 것으로 알려져 있는데, 우리나라의 경우 자작나무 자생지는 해발고도 1,100m 이상에만 나타난다. 하지만, 우리는 점봉산의 거제수나무에서 차가버섯을 채집할 수 있었으며, 스트렙토마이신을 첨가한 PDA 배지를 이용하여 균주를 분리할 수 있었다. 차가 균은 5개 부위 중 차가 균으로 만연된 나무조직에서 분리될 뿐 다른 부분에서는 분리가 어려웠다. 균핵에서 확인한 포자는 $6.0{\sim}10.0{\times}4.5{\sim}6.0{\mu}m$ 크기를 나타내었고, 균사 굵기는 $2.5{\sim}5.0{\mu}m$ 이었다. 실내 배양에서 균총은 불규칙한 모양을 띄며, 시간이 경과할수록 균총의 중간 중간이 노란색으로 변색되었다. 약 20일이 경과하면 노란색으로 변색된 부분에서 적갈색의 강모(setae)를 확인할 수 있으며, 강모의 크기는 $4{\sim}6{\times}100{\sim}420{\mu}m$ 이었다. 차가의 균사생장에 가장 좋은 배지는 OA(oatmeal agar)이었다. 적합한 온도 범위는 $25{\sim}30^{\circ}C$이었으며, 균사생장에 적합한 pH는 8.0~9.5로 비교적 높은 산도를 좋아하는 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제31권11호
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• pp.1818-1827
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• 2018

Objective: The objective of this work was to determine the prevalence of the pathologies that caused the condemnation of pig carcasses in an area of intensive pig farming and Mediterranean climatology and to evaluate their influence in a risk-based inspection procedure for slaughterhouses. Methods: A retrospective observational investigation was carried out from 2002 to 2016 into the pathological processes that caused the condemnation of pig carcasses in a slaughterhouse from South-eastern Spain. The seasonal effect on the causes of condemnation carcass was reported. Negative binomial model was used to evaluate the effect of season on the rate of antemortem rejections and post-mortem condemnations. Histopathological examinations were performed to confirm the diagnosis. Results: The risk of antemortem rejections (0.0564%) was significantly greater in summer (risk ratio [RR] = 1.57). Autumn was associated with higher rate (RR = 1.69) of the total postmortem condemnations (0.1046%). Significantly higher rates of pronounced anaemia (0.0111%) were observed in summer (RR = 3.20). The main causes of anaemia were observed gastroesophageal ulcers and haemorrhagic enteropathies. Significantly highest risk of erysipelas (0.0074%) were observed in autumn (RR = 5.485). About other zoonosis, only eight cases (0.0013%) of carcasses were declared unfit due to tuberculosis lesions. Porcine muscular cysticercosis was not detected. Nevertheless, nonspecific causes such as generalized infections and emaciation represented the half of the condemned carcasses (50.90%). Conclusion: The pathologies leading to the condemnation of carcasses in this study can be considered representative of the pathologies that affect the pig population from a region with a high intensive production and Mediterranean climatology because this slaughterhouse receives a lot of animals from many farms of different size in a high intensive pig production zone (Mediterranean region). Increased knowledge of environmental factors that may foment the appearance of the diseases is essential for implementing inspection programs based on risk assessment in pig's slaughterhouses.

• International Journal of Oral Biology
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• 제34권2호
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• pp.61-72
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• 2009

Chios gum mastic (CGM) is a resin produced from the stem and leaves of Pistiacia lentiscus L var chia, a plant which grows only on Chios Island in Greece. CGM has been used for many centuries as a dietary supplement and folk medicine for stomach and duodenal ulcers in many Mediterranean countries and is known also to induce cell cycle arrest and apoptosis in some cancer cells. In this study, we further investigated the induction and mechanisms underlying the apoptotic response to CGM treatment in the SCC25 human tongue squamous cell carcinoma cell line. The viability of SCC25 cells, human normal keratinocytes (HaCaT cells) and human gingival fibroblasts (HGF-1 cells), and the growth inhibition of SCC25 cells were assessed by MTT assay and clonogenic assay, respectively. Staining with Hoechst and hemacolor dyes and TUNEL assays were employed to detect SCC25 cells undergoing apoptosis. SCC25 cells were treated with CGM, and this was followed by western blotting, immunocytochemistry, confocal microscopy, FACScan flow cytometry, MMP activity and proteasome activity analyses. CGM treatment of SCC25 cells was found to result in a time- and dosedependent decrease in cell viability, a dose-dependent inhibition of cell growth, and apoptotic cell death. Interestingly, CGM showed a remarkable level of cytotoxicity in SCC25 cells but not in normal cells. Tested SCC25 cells also showed several lines of apoptotic manifestation. Taken together, our present findings demonstrate that CGM strongly inhibits cell proliferation by modulating the expression of G1 cell cycle-related proteins and induces apoptosis via the proteasome, mitochondria and caspase cascades in SCC25 cells.

• 대한한의학원전학회지
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• 제22권3호
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• pp.31-51
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• 2009

The characteristics of Jin's ideas on clinic theory can be arranged as follows. 1. Jin emphasized warming and tonifying[溫補] in treatment and the part that shows this the best is the taking care of[調理] the Vital gate[命門], kidney, liver, and spleen. His ideas were based on his understanding of a human life's origin, and was influenced by Seolgi(薛己), Joheon-ga(趙獻可) and Janggaebin(張介賓)'s Vital gate and source Gi theory(元氣說) so scholastically, he has that in common with them but was later criticized by later doctors such as Oksamjon(玉三尊) as an 'literary doctor(文字醫)' who followed the ideas of "Uigwan(醫貫)". 2. The warming and tonifying school[溫補學派], who were influenced by Taoism, said in their theory of disease outbreak[發病學說] that since one must not hurt one's Yin essence and Yang fire [陰精陽火] there is more deficiency than excess, so that was why they used tonifying methods. Jin was also like them and this point of view is universal in internal medicine, gynecology, pediatric medicine and surgery and so on. 3. Jin, who saw the negative form of pulse diagnosis[診脈] emphasized following symptoms over pulse diagnosis using the spirit of ‘finding truth based on truth[實事求是]' in "Maekgyeolcheonmi(脈訣闡微)", but emphasized 'the combination of pulse and symptoms[脈證合參]'. He understood pulse diagnosis as a defining tool for symptoms, and in "Seoksilbirok(石室秘錄)" simplified pulse diagnosis into 10 methods : floating/sunken(浮沉), slow/fast(遲數), large/fine(大小), vacuous/replete(虛實) and slippery/rough(滑澀). 4. Jin used 'large formulas(大方)' a lot that usually featured a large dose, and in " Bonchosinpyeon(本草新編)" he thought of the seven formulas(七方) and ten preparations(十劑) as the standard when using medicine. He did away with old customs and presented a 'new(新)' and 'extra(奇)' point of view. He especially used a lot of Insam(人蔘) when tonifying Gi and Geumeunhwa(金銀花) when treating sores and ulcers. 5. In the area of surgery Jin gave priority to the early finding and treatment of disease with internal treatment[內治] and was against the overuse of acupuncture. However records of surgical measures in a special situation like lung abscesses(肺癰) and liver abscesses(肝癰), and anesthetic measures using 'Manghyeongju(忘形酒)' and 'Singoiyak(神膏異藥)' and opening the abdomen or skull, and organ transplants using a dog's tongue are important data. 6. Jin stated the diseases of Gi and blood broadly. Especially in the principles of treating blood, blood diseases had to be forwarded[順] and Gi regulation[理氣] was the number one priority and stated the following two treatments. First, in "Jeonggiinhyeolpyeon(精氣引血篇)" of volume 6 of "Oegyeongmieon(外經微言)", for the rules for treating blood he stated the pattern identification of finding Gi in blood and blood in Gi. Second, he emphasized Gi regulation(理氣) in blood diseases and stated that the Gi must be tonifyed after finding the source of the loss of blood.

• 대한의생명과학회지
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• 제6권2호
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• pp.119-129
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• 2000

피부는 인체의 표면을 보호하는 중요한 기관으로 피부가 손상되었을 경우 상처 재생은 염증기, 상피화기, 수복기의 정상적인 재생 단계를 거치며 치유된다 최근 저강도 레이저의 생물학적 효과로서 상처 재생과의 밀접한 관련성이 알려지고 있다. 본 연구는 저강도 레이저가 상처 재생에 미치는 유의한 효과를 세포 형태학적으로 확인하기 위해 실험적으로 유도한 가토 피부 상처 (2$\times$2 cm)에 12일 동안 5 Hz, 830 nm, 1.6 J/$cm^2$의 자극강도 (10 min/day)로 상처면에 레이저를 적용한 결과, 다음과 같은 곁과를 얻었다. 레이저 조사군의 경우 결합조직의 수복과 상피의 재형성이 대조군과 비교했을 매우 빠르게 진행되는 것으로 관찰되었으며, 특히 섬유아세포의 활성과 육아조직 합성율이 유의하게 증가되는 것으로 확인되었다. 이상의 연구 곁과를 종합해 달 때 유효한 치료강도의 저강도 레이저 자극은 피부의 개방성 창상 및 욕창 등의 상처 치유를 촉진할 수 있는 것으로 사료된다.