• Title/Summary/Keyword: ubiquinones

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Antioxidant Properties and Ubiquinone Contents in Different Parts of Several Commercial Mushrooms (시판버섯의 부위별 항산화능과 유비퀴논 함량)

  • Hong, Myung-Hee;Jin, Yoo-Jeong;Pyo, Young-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.9
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    • pp.1235-1241
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    • 2012
  • Antioxidant properties and antioxidant compound contents in different parts of 14 commercial mushrooms were evaluated. Methanolic extracts from the entire mushroom, the pileus and the stipe, separately, were screened for their DPPH and ABTS free radical scavenging activities. Total ubiquinones (Coenzyme Qs; CoQs), total phenolic, and flavonoid contents were determined, in order to assess the extract's antioxidant activity. The portion of the mushroom selected had an effect on the results, with pileus methanolic extract exhibiting the greatest antioxidant effect (p<0.05). The analyzed mushrooms contained powerful antioxidants such as phenols (144.5~536.6 mg of gallic acid equivalents, mg GAE/100 g of dried weight, dw), flavonoids (3.7~31.2 mg of quercetin equivalents, mg QE/100 g dw) and ubiquinones (65.6~485.1 ${\mu}g$/100 g dw). Content of CoQ9 and CoQ10 in the 14 commercial mushrooms varied from 23.1 to 256.2 ${\mu}g$/100 g and from 16.1 to 238.3 ${\mu}g$/100 g, respectively. Phellinus linteus showed the highest antioxidant activity among all species due to the contribution of antioxidants such as phenols (530.5 mg GAE/100 g dw) and ubiquinones (308.8 ug/100 g dw). A positive linear correlation was demonstrated between free radical scavenging activity and total phenolic ($R^2=0.79$) and ubiquinone ($R^2=0.59$) contents in the pileus of mushrooms (p<0.05). Our data indicate that commercial mushrooms have potential as dietary sources of CoQs and phenolic antioxidants.

Analysis of the Seasonal Change in Attached Algae and Microbial Community Structure in Sediment Basin Trough of Water Treatment Plant By Using Quinone Profile and PCR-DGGE (Quinone profile과 PCR-DGGE를 이용한 정수장 침전지에서의 부착조류 및 미생물군집의 계절적 변화해석)

  • Yu, Hyun-Sun;Lim, Byung-Ran;Ahn, Kyu-Hong
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.3
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    • pp.461-467
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    • 2006
  • The seasonal change in attached algae and microbial community structure at sedimentation basin of water treatment plant was investigated by using quinone profiles and denaturing gel gradient electrophoresis (DGGE). The photosynthetic bacteria and algae contains PQ-9 and VK-1 as major quinone are major component of the total quinone fraction in attached algae and microorganisms on sedimentation basin trough. The microorganisms containing menaquinones appear to be sensitivity to the change in temperature than those containing ubiquinones. The plot of the mole fraction of dominant quinone species ($f_d$) to the DQ values showed higher sensitivity to the seasonal change in the microbial community structure. The results indicated that quinone and DGGE are useful tool for the evaluation of the changes in the microbial community structure.

Characterization of Microbial Community in Biological Wastewater Treatment System Using Respiratory Quinone Profiles

  • Lim Byung-Ran;Ahn Kyu-Hong;Lee Yonghun
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2003.05a
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    • pp.111-114
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    • 2003
  • The dynamics of microbial community structure of the various domestic wastewater treatment processes were examined using a novel approach of quinone profiles. The compositions of microbial quinone of 5 sites fer plant and lab-scale activated sludge were analyzed. More than 14 kinds of quinones were observed in the activated sludges tested in this study. The microbial community structure of the plant activated sludge processes a little differed from that of the lab-scale submerged MBR systems. The dominant quinones were UQ-8, UQ-10 followed $MK-8(H_4)$, MK-7 and MK-6. The molar ratio of ubiquinones to menaquinones (UQ/MK) changed from 0.81 to 1.9, indicating that aerobic bacteria dominated the microbial community of the activated sludge examined. The microbial diversity of the activated sludges calculated from the all quinone compositions was 9.5-11.9 and the microbial equability of the activated sludges was 0.64-0.79.

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Change of Microbial Community on Ozonation of Sewage Sludge to Reduce Excess Sludge Production (폐슬러지 감량화 및 재활용을 위한 오존 처리시 하수슬러지내의 미생물 군집구조의 변화)

  • Hong, Jun-Seok;Lim, Byung-Ran;Ahn, Kyu-Hong;Maeng, Sung-Kyu
    • Journal of Korean Society of Water and Wastewater
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    • v.18 no.1
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    • pp.59-65
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    • 2004
  • The change of the microbial community structure in excess sludge of different sewage treatment plants by ozone treatment was investigated by quinone profiles. The resulting ozone dosage ranged from 0.1 to $0.4gO_3/gTS$. In terms of overall sludge reduction, more than 50% reduction of the total sludge mass could be achieved by ozone treatment at $0.4gO_3/gTS$. Quinone concentration and type in sludge of different treatment plants were remarkably decreases with increasing ozone dose. Ubiquinones(UQs)-8, -10 and MK-8 were still remained in the ozonized sludge at $0.4gO_3/gTS$. The results of this study showed that the remaining microorganisms belong to UQs-8, -10 and MK-8 were difficult to destruct cell membrane or wall by ozonation. Fecal Streptococci and Salmonella were not detected at ozone dose of $0.2gO_3/gTS$, but Fecal Coliform was not detected at ozone dose of $0.4gO_3/gTS$.