• Kyungpook Mathematical Journal
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• 제53권4호
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• pp.647-660
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• 2013

In the paper, a two-prey one-predator system with defensive ability and Holling type-II functional responses is investigated. First, the stability of equilibrium points of the system is discussed and then conditions for the persistence of the system are established according to the existence of limit cycles. Numerical examples are illustrated to attest to our mathematical results. Finally, via bifurcation diagrams, various dynamic behaviors including chaotic phenomena are demonstrated.

• IMMUNE NETWORK
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• 제5권2호
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• pp.68-77
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• 2005

Background: Costimulation is a critical process in Ag-specific immune responses. Both B7.1 and CD28 molecules have been reported to stimulate T cell responses during antigen presentation. Therefore, we tested whether Ag-specific immune responses as well as protective immunity are influenced by coinjecting with B7.1 and CD28 cDNAs in a mouse HSV-2 challenge model system. Methods: ELISA was used to detect levels of antibodies, cytokines and chemokines while thymidine incorporation assay was used to evaluate T cell proliferation levels. Results: Ag-specific antibody responses were enhanced by CD28 coinjection but not by B7.1 coinjection. Furthermore, CD28 coinjection increased IgG1 production to a significant level, as compared to pgD+pcDNA3, suggesting that CD28 drives Th2 type responses. In contrast, B7.1 coinjection showed the opposite, suggesting a Th1 bias. B7.1 coinjection also enhanced Ag-specific Th cell proliferative responses as well as production of Th1 type cytokines and chemokines significantly higher than pgD+pcDNA3. However, CD28 coinjection decreased Ag-specific Th cell proliferative responses as well as production of Th1 types of cytokines and chemokine significantly lower than pgD+pcDNA3. Only MCP-1 production was enhanced by CD28. B7.1 coimmunized animals exhibited an enhanced survival rate as well as decreased herpetic lesion formation, as compared to pgD+pcDNA3. In contrast, CD28 vaccinated animals exhibited decreased survival from lethal challenge. Conclusion: This study shows that B7.1 enhances protective Th1 type cellular immunity against HSV-2 challenge while CD28 drives a more detrimental Th2 type immunity against HSV-2 challenge, supporting an opposite role of B7.1 and CD28 in Ag-specific immune responses to a Th1 vs Th2 type.

• 생물정신의학
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• 제2권2호
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• pp.275-280
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• 1995

Smooth pursuit eye movement, one of the reliable biological markers of schizophrenia, is not always abnormal in schizophrenic patients. Therefore the authors studied it in schizophrenic patients and normal controls and compared the results according to the presence or abscence of family history of psychosis and types (type I and type II). The results are as follows: 1) In the 18 normal control group (8 mole, 10 female), there was no sex difference in the responses of smooth pursuit eye movement. 2) In th 44 schizophrenic group (28 male, 16 female), there also was no sex difference in the responses of smooth pursuit eye movement. 3) In comparison of 44 schizophrenic group to 18 normal control group, there was significantly increased abnormal response in smooth pursuit eye movement in schizophrenic group (P < 0.005). 4) In schizophrenic group, there was no difference in the responses of smooth pursuit eye movement between type I and type II schizophrenia. 5) The presence or abscence of family history of psychosis made no difference in the responses of smooth pursuit eye movement in schizophrenic group. 6) Subdivision of type I or type II in each case of presence or abscence of family history made no difference in the responses of smooth pursuit eye movement in schizophrenic group.

• 아동학회지
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• 제25권5호
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• pp.179-191
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• 2004

An emotion inference task was used to investigate children's cognitive cuing by age, cue type, and situation-emotion fit. Subjects were 41 of 3-, and 5-year-old children from two different day-care centers in Seoul and Kyonggi province. Each child was individually interviewed with pictorial tasks. 5-year-old children demonstrated more cuing, thinking, and application responses than 3-year-old children. Particularly, they showed more situation and thinking responses in situation-emotion match than in situation-emotion mismatch. 3-year-old children showed more past-oriented responses than 5-year-old children. In the interpersonal cue story, there were more situation, past-oriented and thinking responses than in the interobjective ones. The interpersonal cue story produced more situation responses in situation-emotion match than in situation-emotion mismatch.

• IMMUNE NETWORK
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• 제11권5호
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• pp.268-280
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• 2011

Background: Dengue virus, which belongs to the Flavivirus genus of the Flaviviridae family, causes fatal dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) with infection risk of 2.5 billion people worldwide. However, approved vaccines are still not available. Here, we explored the immune responses induced by alternating prime-boost vaccination using DNA vaccine, adenovirus, and vaccinia virus expressing E protein of dengue virus type 2 (DenV2). Methods: Following immunization with DNA vaccine (pDE), adenovirus (rAd-E), and/or vaccinia virus (VV-E) expressing E protein, E protein-specific IgG and its isotypes were determined by conventional ELISA. Intracellular CD154 and cytokine staining was used for enumerating CD4+ T cells specific for E protein. E protein-specific CD8+ T cell responses were evaluated by in vivo CTL killing activity and intracellular IFN-${\gamma}$ staining. Results: Among three constructs, VV-E induced the most potent IgG responses, Th1-type cytokine production by stimulated CD4+ T cells, and the CD8+ T cell response. Furthermore, when the three constructs were used for alternating prime-boost vaccination, the results revealed a different pattern of CD4+ and CD8+ T cell responses. i) Priming with VV-E induced higher E-specific IgG level but it was decreased rapidly. ii) Strong CD8+ T cell responses specific for E protein were induced when VV-E was used for the priming step, and such CD8+ T cell responses were significantly boosted with pDE. iii) Priming with rAd-E induced stronger CD4+ T cell responses which subsequently boosted with pDE to a greater extent than VV-E and rAd-E. Conclusion: These results indicate that priming with live viral vector vaccines could induce different patterns of E protein-specific CD4+ and CD8+ T cell responses which were significantly enhanced by booster vaccination with the DNA vaccine. Therefore, our observation will provide valuable information for the establishment of optimal prime-boost vaccination against DenV.

• Clinical and Experimental Pediatrics
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• 제48권3호
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• pp.251-259
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• 2005

Atopy is a highly prevalent and serious health problem. The prevalence and severity of asthma and allergic diseases have increased over recent decades, particularly in industrialized nations. Early life infections may protect against the development of atopy and allergic diseases like asthma. The inverse relationship between the incidence of atopy and childhood infections has led to the 'hygiene hypothesis', which suggests that diminished exposure to childhood infections in modern society has led to decreased Th1-type responses. Th1 and Th2 responses are counter-regulatory. Reduced Th1 may lead to enhanced Th2-type inflammation, which is important in promoting asthma and allergic disease via up-regulation of IL-4, IL-5, and IL-13. It is now widely accepted that altered regulation of Th2 responses(and possibly the balance between Th1 and Th2 responses) is an important factor in the development of atopy. CpG DNA represent a novel class of drugs with substantial immunomodulatory properties. CpG DNA contain unmethylated motifs centered on the CpG dinucleotides, like bacterial DNA. These CpG DNA promote Th1 and regulatory type immune responses and suppress Th2 responses. In murine studies, CpG DNA are effective in prevention and treatment of asthma and allergic diseases. CpG DNA are just beginning to be tested in human asthma. While its precise mechanisms continue to be fully studied, CpG DNA offers considerable promise as a novel treatment for atopic inflammation. It may prove to be an important disease modifying therapy, or even curative therapeutic agent for asthma and allergic diseases.

• Fisheries and Aquatic Sciences
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• 제13권1호
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• pp.56-62
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• 2010

To determine whether long double-stranded RNA (dsRNA) induces RNA interference and type I interferon (IFN) responses in fish, long dsRNAs encoding enhanced green fluorescent protein (EGFP), GFPuv, and polyinosinic-polycytidylic acid sequences were co-injected with an EGFP expressing plasmid, into rock bream (Oplegnathus fasciatus). We investigated the EGFP mRNA and protein levels, and the transcriptional responses of dsRNA-dependent protein kinase and Mx1 genes. Long dsRNAs were strong inducers of a type I IFN response in rock bream, resulting in nonspecific suppression of exogenous gene expression. Furthermore, sequence-specific knockdown of exogenous gene expression at the mRNA level was detected at an early phase (24 h). These results suggested that long dsRNA may inhibit exogenous gene expression through an early mRNA interference response and a later type I IFN response in fish.

• 한국학교ㆍ지역보건교육학회지
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• 제20권2호
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• pp.81-91
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• 2019

Objectives: It helps dental clinic to work 1:1 consulting and select the properties of the Kakao Talk message. The study was designed to determine the influence of users cognitive and emotional responses by message attribute that affect intention of use of dental clinic. Methods: This study targeted 297 peple Kakao Talk users aged 20-50 s, in Seoul, Gyeonggi-do and Incheon from, February 6 to April 7, 2017. We analyzed the data with frequency analysis, t-test, one-way ANOVA, and multiple regression analysis by using SPSS ver 24.0(SPSS Inc, Chicago, USA). The significance level for significance was set at 0.05. Results: Among the user's responses about the event type Kakao talk message, the ease(${\beta}=0.121$, p<0.01), usefulness(${\beta}=0.148$, p<0.001), affinity(${\beta}=0.471$, p<0.001), satisfaction(${\beta}=0.249$, p<0.001). affected intention of use of dental clinic. Among the user's responses about the dental information type, the ease(${\beta}=0.150$, p<0.05), expertise(${\beta}=0.151$, p<0.05), satisfaction(${\beta}=0.237$, p<0.001) affected intention of use of dental clinic(p<0.05). Among the user's responses about the general public relations type, the expertise(${\beta}=0.254$), affinity(${\beta}=0.193$), satisfaction(${\beta}=0.474$) affected intention of use of dental clinic(p<0.001). Conclusion: It is necessary to elicit a positive intention of use of dental clinic through a highly emotional response from the users to improve the image of the dental clinic and to attract patients.

• 제어로봇시스템학회논문지
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• 제9권5호
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• pp.379-383
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• 2003

Parallel type fuzzy controller is designed by using a hybrid connected type fuzzy-PID controller and a model reference fuzzy controller. The first controller, consists of a fuzzy-PI and a fuzzy-PD making a hybrid type fuzzy-PID controller, plays a role as firstly reaching stable responses and secondly overcoming disturbance in plants. The second controller, model reference fuzzy controller, plays a role as reaching faster responses than other controllers. We have confirmed that the controller produces rapid and stable responses and overcomes disturbance by using parallel type fuzzy controller in a DC motor application.

• Structural Monitoring and Maintenance
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• 제9권2호
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• pp.129-155
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• 2022

In this study, parametric analyses on a hoop-type PZT (lead-zirconate-titanate) interface are performed to estimate the effects of the PZT interface's materials and geometries on sensitivities of impedance responses under strand breakage. The paper provides a guideline for installing the PZT interface suitable in tendon anchorages for damage-sensitive impedance signatures. Firstly, the concept of the PZT interface-based impedance monitoring technique in prestressed tendon anchorage is briefly described. A FE (finite element) analysis is conducted on a multi-strands anchorage equipped with a hoop-type PZT interface for analyzing materials and geometric effects. Various material properties, geometric sizes of the interface, and PZT sensor are simulated under two states of prestressing force for acquiring impedance responses. Changes in impedance signals are statistically quantified to analyze the effect of these factors on damage-sensitive impedance monitoring in the tendon anchorage. Finally, experimental analyses are performed to demonstrate the effects of materials and geometrical properties of the PZT interface on damage-sensitive impedance monitoring.