• International Journal of Industrial Entomology and Biomaterials
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• 제47권2호
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• pp.126-133
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• 2023

Genetic resources of mulberry trees are commonly preserved as trophosomes, which are vulnerable to environmental factors, such as natural disasters, diseases, and pests. This study establishes a basic protocol for ultra-low temperature cryopreservation of mulberry trees using a two-step freezing process. The procedure was established using the "Daeshim" variety and then tested on genetic resources from 24 other mulberry varieties. Samples were first dried to a moisture content of 33-43% in a low-temperature forced-air chamber at -5 ℃, then slowly frozen from -5 ℃ to -20 ℃, and preserved in liquid nitrogen (-196 ℃). To determine the regeneration rate, isolated dormant buds were inoculated into MS basal medium, and grown shoots were grafted onto 1-year-old rootstock via chip budding and then cultured. After freezing in liquid nitrogen, the "Daeshim" variety exhibited a survival and regeneration rate of more than 70% and 50%, respectively. Applying the two-step freezing process to genetic resources from 24 mulberry species yielded average survival and regeneration rates of 85.3% and 75.5%, respectively. Morus alba showed survival and regeneration rates of 100%, confirming the efficacy of the two-step freezing method. These results indicate the high feasibility of ultra-low-temperature cryopreservation through two-step freezing of dormant buds from mulberry genetic resources. Additional research is required into the variations in regeneration rates with freezing period in liquid nitrogen.

• 한국표면공학회지
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• 제53권4호
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• pp.144-152
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• 2020

In the manufacturing process of joining of aluminum alloy and polymer, the strength of the metal-polymer joining is greatly influenced by the nanostructure of the oxide film. In this study, we investigated the dependence of joining strength on the thickness, structure, pore formation and surface roughness of the formed film. After the two-step anodization process, the surface oxide layer became thinner and rougher resulting in higher joining strength with the polymer. More specifically, after the two-step anodization, the surface roughness, Ra increased from 2.3 to 3.2 ㎛ with pore of three-dimensional (3D) nanostructure, and the thickness of the oxide film was thinned from 350 to 250 nm. Accordingly, the joining strength of the aluminum alloy with polymer increased from 23 to 30 MPa.

• Bulletin of the Korean Chemical Society
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• 제31권12호
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• pp.3693-3696
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• 2010

We experimentally measure the kinetic energy and angular distributions of photoelectrons of $N_2$ as a function of 410 nm femtosecond laser intensity by using velocity map imaging technique. The strong-field multiphoton ionization of molecules shares many of the characteristics with those of atoms. Electron kinetic energies are nearly independent of laser intensities. The independence suggests that the electron peaks in the photoelectron spectrum actually result from a two-step process, indicative of the occurrence of real population in the intermediate states. The relative amplitudes of electron peaks indicate that in the two-step process, nonresonant population transfer dominates for low intensities, while resonant population transfer dominates for higher intensities.

• Membrane and Water Treatment
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• 제11권1호
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• pp.25-29
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• 2020

The purpose of this study was to investigate the characteristics of lignin fractionated from waste wood (WW) using a two-step process of ethanol organosolv pretreatment followed by ultrafiltration with membranes of different molecular weight cut-offs (1, 5 and 20 kDa). The different permeates obtained were characterized by fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TGA) and gel permeation chromatography (GPC). The analysis by FT-IR and NMR of these lignins showed that the lignin core was successfully separated from WW. TGA curves confirmed that the thermal properties of lignin fractionated by ultrafiltration were almost identical to each other. The results from GPC confirmed that fractionating of lignin was achieved by ultrafiltration. For the membrane fractionation process, values of molecular weight decreased as the cut-offs used to obtain the fractions became smaller. As a result, fractionating lignin by a two-step process allowed separating different fractions of lignin of different molecular weights yielded high purity without interference from existing pollutants in WW. The two-step process offers the possibility of using fractionated WW as an untapped source of lignin.

• 한국수소및신에너지학회논문집
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• 제23권5호
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• pp.559-571
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• 2012

Dimethyl ether (DME) is a new clean fuel as an environmentally-benign energy resource. DME can be manufactured from various energy sources including natural gas, coal, and biomass. In addition to its environmentally friendly properties, DME has similar characteristics to those of LPG. The aim of this article is to represent the development of new DME process with KOGAS's own technologies. KOGAS has investigated and developed new innovative DME synthesis process from synthesis gas in gaseous phase fixed bed reactor. DME has been traditionally produced by the dehydration of methanol which is produced from syngas, a product of natural gas reforming. This traditional process is thus called the two-step method of preparing DME. However, DME can also be manufactured directly from syngas (single-step). The single-step method needs only one reactor for the synthesis of DME, instead of two for the two-step process. It can also alleviate the thermodynamic limitations associated with the synthesis of methanol, by converting the produced methanol into DME, thereby potentially enhancing the overall conversion of syngas into DME. KOGAS had launched the 10 ton/day DME demonstration plant project in 2004 at Incheon KOGAS LNG terminal. In the mid of 2008, KOGAS had finished the construction of this plant and has successively finished the demonstration plant operation. And since 2008, we have established the basic design of commercial plant which can produce 3,000 ton/day DME.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2007년도 추계학술대회 논문집
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• pp.525-526
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• 2007

Over-polishing is required to completely remove the material of top surface across whole wafer, in spite of a local dishing problem. This paper introduces the two-step CMP process using protective layer and high selectivity slurry, to reduce dishing amount and variation. The 30nm thick protective oxide layer was deposited on the pattern, and then polished with low selectivity slurry to partially remove the projected area while suppressing the removal rate of the recessed area. After the first step CMP process, high selectivity slurry was used to minimize the dishing amount and variation in pattern structure. Experimental result shows that two-step CMP process can be successfully applicable to reduce the dishing defect generated in over-polishing.

• 한국축산식품학회지
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• 제37권1호
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• pp.123-133
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• 2017

The development of a new manufacturing process, a two-step temperature treatment, to modulate the physicochemical properties of nanoparticles including the size is critical. This is because its physicochemical properties can be key factors affecting the cellular uptake and the bioavailability of bioactive compounds encapsulated in nanoparticles. The aims of this study were to produce (beta-lactoglobulin) ${\beta}-lg$ nanoparticles and to understand how two-step temperature treatment could affect the formation and physicochemical properties of ${\beta}-lg$ nanoparticles. The morphological and physicochemical properties of ${\beta}-lg$ nanoparticles were determined using atomic force microscopy and a particle size analyzer, respectively. Circular dichroism spectroscopy was used to investigate the secondary structure of ${\beta}-lg$. The surface hydrophobicity and free thiol groups of ${\beta}-lg$ were increased with a decrease in sub-ambient temperature and an increase in mild heat temperature. As sub-ambient temperature was decreased, a decrease in ${\alpha}-helical$ content and an increase in ${\beta}-sheet$ content were observed. The two-step temperature treatment firstly involved a sub-ambient temperature treatment from 5 to $20^{\circ}C$ for 30 min, followed secondly by a mild heat temperature treatment from 55 to $75^{\circ}C$ for 10 min. This resulted in the production of spherically-shaped particles with a size ranging from 61 to 214 nm. Two-way ANOVA exhibited the finding that both sub-ambient and mild heat temperature significantly (p<0.0001) affected the size of nanoparticles. Zeta-potential values of ${\beta}-lg$ nanoparticles were reduced with increasing mild heat temperature. In conclusion, two-step temperature treatment was shown to play an important role in the manufacturing process - both due to its inducement of the conformational changes of ${\beta}-lg$ during nanoparticle formation, and due to its modulation of the physicochemical properties of ${\beta}-lg$ nanoparticles.

• International Journal of Advanced Culture Technology
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• 제4권4호
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• pp.75-80
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• 2016

The process of extracting external boundary of an object is a very important process for recognizing an object in the image. The proposed extraction method consists of two processes: External Boundary Extraction and Thinning. In the first step, external boundary extraction process separates the region representing the object in the input image. Then, only the pixels adjacent to the background are selected among the pixels constituting the object to construct an outline of the object. The second step, thinning process, simplifies the outline of an object by eliminating unnecessary pixels by examining positions and interconnection relations between the pixels constituting the outline of the object obtained in the previous extraction process. As a result, the simplified external boundary of object results in a higher recognition rate in the next step, the object recognition process.

• 전기학회논문지P
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• 제59권1호
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• pp.53-57
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• 2010

Low-density parity-check (LDPC) codes with belief-propagation (BP) algorithm achieve a remarkable performance close to the Shannon limit at reasonable decoding complexity. Conventionally, each iteration in decoding process contains two steps, the horizontal step and the vertical step. In this paper, an efficient implementation of the adaptive offset min-sum (AOMS) algorithm for decoding LDPC codes using the single-step method is proposed. Furthermore, the performances of the AOMS algorithm compared with belief-propagation (BP) algorithm are investigated. The algorithms using the single-step method reduce the implementation complexity, speed up the decoding process and have better efficiency in terms of memory requirements.

• 대한전자공학회논문지
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• 제17권5호
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• pp.22-27
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• 1980

새로운 불순물(dopant) 원료인 스핀온 쏘스를 사용하여 붕소를 실리콘천에 이단계 확산시키는 공정을 검토하였다. predeposition후의 확산충의 두께의 drive-in 확산후의 두께에 비하여 무시할 수 없이 큰 경우에 대하여 이단계 확산공정이 끝난 후의 확산층의 두께를 예측할 수 있는 간단한 방법을 제안하였다. 제안된 방법에 의하여 불순물 확산충의 두께를 예측하였으며 그 결과를 실험적으로 측정된 값과 비교하였다.