• International Journal of Advanced Culture Technology
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• 제4권4호
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• pp.75-80
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• 2016

The process of extracting external boundary of an object is a very important process for recognizing an object in the image. The proposed extraction method consists of two processes: External Boundary Extraction and Thinning. In the first step, external boundary extraction process separates the region representing the object in the input image. Then, only the pixels adjacent to the background are selected among the pixels constituting the object to construct an outline of the object. The second step, thinning process, simplifies the outline of an object by eliminating unnecessary pixels by examining positions and interconnection relations between the pixels constituting the outline of the object obtained in the previous extraction process. As a result, the simplified external boundary of object results in a higher recognition rate in the next step, the object recognition process.

• Journal of Plant Biology
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• 제39권1호
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• pp.63-69
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• 1996

인삼의 모상근 HRB-15 clone을 3L 용량의 생물반응기에서 배양하여 ginsenoside 생산량을 증진시킬 수 있는 방법을 찾고자 본 연구를 수행하였다. 모상근을 광배양하면 암배양에 비해 생장이 30% 정도 억제되지만 ginsenoside 함량(ginsenoside-Rb1, -Rb2, -Rc, -Rd, -Re, -Rg1)은 크게 증가되어 암배양시보다 2배 높은 1.10%로 나타났으며, 이때에는 ginsenoside-Re의 함량이 매우 높은 반면 다른 ginsenoside들의 함량은 암배양시 보다 다소 감소하는 경향이었다. 또한 초기 10일간 암배양한 후 20일간 광배양하는 방법을 기준으로 sodium acetate를 농도별로 처리하여 본 결과, sodium acetate의 농도가 증가할수록 모상근의 생장이 억제되고 ginsenoside 함량도 감소하였으며, 배양배지내 인산의 농도를 변화시켜 본 결과, 1.25 mM $KH_2PO_4$ 처리가 모상근의 생장과 ginsenoside 함량 모두에서 가장 높게 나타났다. 한편 모상근의 생장과 ginsenoside 함량을 증가시키기 위해 yeast elicitation과 ammonium nitrate를 이용한 2단계 배양방법(two-step culture process)을 개발하였다. Elicitor로서 $50\;\mu\textrm{g}/g$ yeast extract를 배양 20일째(광배양 10일째)에 첨가하여 본 결과, 총 ginsenoside 생산 모두에서 상승된 효과를 보여주었다. 따라서 2단계 배양방법은 인삼 모상근의 대량배양을 통해 이차대사산물인 ginsenoside 생산량을 증가시킬수 있는 하나의 좋은 방법으로 생각된다.

• 디지털산업정보학회논문지
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• 제14권4호
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• pp.189-204
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• 2018

The purpose of this study is to examine an organizational performance difference by individual utilization level of the smart work. The Smart work help minimizing business process and improving organization productivity based on information technology. This new technology provides a flexible way of the task through smart-work center, videoconferencing, telecommuting, mobile(remote control) and business-only messenger. This investment changes organizational culture, institutions and behavior by new technology applying. The organization system change due to smart work has trouble between alteration preferences and existing maintains a group. In response, the organization should make investment justification of smart work for institutional and culture stabilized by a new system in organization. I set up the analytical process of four stages for empirical research. It will analyze an operation difference of the smart work between pre and post investment in the first-step analysis. The two-step analysis will conduct a text mining analysis of smart work operations. The three-step analysis will identify organization performance differences among individual levels in smart work. The four-step analysis will identify a factor difference in organizational performance by individual utilization level on smart work. According to the study, It has been revealed a difference between the pre and post investment performance on smart work. The text mining analyses many appeared an improvement opinion of organizational culture. Next, there is a difference in organization performance among utilize groups of smart work. Furthermore, the factors of organizational performance among groups appeared differently. The theoretical contribution of this study provided to expand the organizational theory of organization change and resistance. The practical implications provided to require a strong guideline an organizational culture and institution for smart work.

• 복식문화연구
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• 제28권4호
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• pp.508-526
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• 2020

This study has established a fashion illustration education plan using the contour drawing that fosters observational ability and enables creative drawing. This study developed two illustration curriculum proposals consisting of 15 weeks, combining literature and case studies. The researchers organized a step-by-step teaching plan that utilizes contour drawing according to the three stages of fashion illustration classes: foundation courses, general courses, and intensive courses. When the contour drawing is used at the beginning stage of the foundation courses of fashion illustration, it can be used as a technique to reduce the fear of students about practical skills, induce interest in illustration, and cultivate observation ability about objects. In general process, it is combined with various tools and coloring materials to strengthen expression power, and it is possible to produce detailed expressions and illustrations about human body and clothing. In intensive courses, it is expanded to the production of creative works with new aesthetics through digital techniques and mixed materials. As such, the contour drawing is expanded in various ways according to the learning contents and goals of each step, and is flexibly adjusted according to the learning content. Contour drawing has the effect of acquiring observation and expression ability, and it is analyzed as a technique that enables the production of creative illustration of students.

• 복식문화연구
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• 제10권1호
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• pp.49-59
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• 2002

The type of product and consumer characteristis have known to play an important role in the post-purchase behavior. The issue related to the effects of clothing type and sex on the process of consumer satisfaction formation, would be helpful to understand the past-purchase consumer behavior. Therefore, the objective of this study was to clarify differences in the process of satisfaction formation in relation to clothing type and sex. The study was conducted in three steps. Through the two steps, measurement instruments were developed. At the last step, judgement sampling method was utilized to collect the data and subjects were 614 university students. Factor analysis and path analysis were used to analyze the data. The process of consumer satisfaction formation in formal wear and casual wear showed some differences. In the case of format wear, only expressive product performance was found to play an important role in the process of consumer satisfaction formation. However in the case of casual wear, both expressive and instrumental product performances had influence upon clothing satisfaction directly or through consumption emotion. The results revealed a similar tendency in the process of satisfaction formation between male and female groups. However in the male group the consumption emotion had a greater effect on the process of consumer satisfaction formation than in the female group it did.

• KSBB Journal
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• 제7권4호
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• pp.308-316
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• 1992

유전자 재조합 균주의 성질이 불안정할 경우,2 단 연속 배양조를 사용하면 여러가지 이점이 있다. 본 논문은 2단 연속 배양조의 최적화를 목적으로 유전자 재조합 대장균의 과도상태 거동을 1단 및 2단 연속 배양조에서 연구한 결과를 다룬다. 희석율이 갑자기 변할 때 대장균은 새로운 희석율에 적응하기 위해 성장속도를 바꾸는데 적응 속도가 성장 잠재력에 비례한다는 가정아래 수학적 이론식을 도출하였으며, 이때 중요한 동력학적 변수는 무차원의 순간속도 증가(a) 빛 적응속도 상수(k)이었다. 이 상수들을 여러 온도와 희석 속도에서 실험적으로 측정하였고, 이 측정값을 모렐식에 적용한 결과 실제 2단 배양조의 미생물 성장속도의 과도기적인 성질을 잘 묘사하는 것으로 나타났다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2006년도 Proceedings of The Convention
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• pp.127-130
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• 2006

Coenzyme Q10(CoQ10) is a biological quinine compound that is widely found in living organisms including yeast, plants, and animals. CoQ10 has two major physiological activities:(a)mitochondrial electron-transport activity and (b )antioxidant activity. Various clinical applications are also available: Parkinson's disease, Heart disease, diabetes. Because of its various application filed, the market size of CoQ10 is continuously expanding all over the world. A Japanese company, Nisshin Pharma Inc. is the first industrial producer of CoQ10(1974). CoQ10 can be produced by fermentation and chemical synthesis. In several companies, these two methods are used for the production of CoQ10:chemical synthesis - Yungjin, Daewoong, Nishin Parma; fermentation - Kaneka, Kyowa, Yungjin, etc. Researchs in microbial production of CoQ10 have several steps: screening of producing microorganisms, strain development, fermentation process, purification process, scale-up process, plant production. Several strategies are available for the strain development : Random mutation and screening, directed metabolic engineering. For the optimization of fermentation process, various conditions (nutrient, aeration, temperature, culture type, etc.) are considered. Purification is one of the most important step because the quality of final products entirely depends on its purity. The production cost will be reduced and the quality of the CoQ10 will be impoved by continuous researches in strain development, fermentation process, purification process.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2006년도 Proceedings of The Convention of The Korean Society of Applied Pharmacology
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• pp.127-130
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• 2006

Coenzyme Q10(CoQ10) is a biological quinine compound that is widely found in living organisms including yeast, plants, and animals. CoQ10 has two major physiological activities:(a)mitochondrial electron-transport activity and (b)antioxidant activity. Various clinical applications are also available : Parkinson's disease, Heart disease, diabetes. Because of its various application filed, the market size of CoQ 10 is continuously expanding all over the world. A Japanese company, Nisshin Pharma Inc. is the first industrial producer of CoQ10(1974). CoQ10 can be produced by fermentation and chemical synthesis. In several companies, these two methods are used for the production of CoQ10:chemical synthesis - Yungjin, Daewoong, Nishin Parma; fermentation - Kaneka, Kyowa, Yungjin, etc. Researchs in microbial production of CoQ10 have several steps: screening of producing microorganisms, strain development, fermentation process, purification process, scale-up process, plant production. Several strategies are available for the strain development : Random mutation and screening, directed metabolic engineering. For the optimization of fermentation process, various conditions (nutrient, aeration, temperature, culture type, etc.) are considered. Purification is one of the most important step because the quality of final products entirely depends on its purity. The production cost will be reduced and the quality of the CoQ10 will be impoved by continuous researches in strain development, fermentation process, purification process.

• KSBB Journal
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• 제15권5호
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• pp.537-540
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• 2000

일반적으로 paclitaxel과 유사한 구조를 가진 복잡한 유도체의 분리나 최종 제품에서의 엄격한 규정 등으로 인하여 한 단계의 HPLC system으로 정 제하는 것은 상당히 어렵다. 이러한 이유로 두 단계 HPLC system으로 식물세포 배양액으로 부터 paclitaxel의 대량 정제를 수행하였다. 즉, 첫번째 단계에 서 reverse-phase HPLC column, 두번째 단계에서 normal­p phase HPLC column을 사용하여 최종 제품을 얻었다. 또한 최종 정 제 된 paclitaxel 내 에 포함되어 있는 불순물 profile 확 언, 함량 분석, 그리고 이들 물질들에 대한 분리, 정제 및 동 정을 실시하였다 이들 불순물들 중에서 0.1% 이상되는 것은 모두 6종 (side chain derivative, baccatin III, 10-deacetyltaxol, cephalomannine, taxane derivative, 7-epitaxol)이며 NMR과 MS를 사용하여 화학구조를 분석한 결과 이미 알려진 pacli­t taxel 유도체 또는 전구체와 동일한 물칠로 밝혀 졌다. 본 연구 결과는 결국 paclitaxel 생산을 위한 품질관리 (quality con­t tr이)와 허가를 위한 자료로 유용하게 사용되어 진다.

• 문화기술의 융합
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• 제8권5호
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• pp.653-662
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• 2022

최근 이미지와 같은 다차원의 복잡한 패턴 인식에 많이 사용하는 CNN(Convolutional Neural Networks)을 적용한 지문분류 방법이 다양하게 연구되고 있다. CNN 기반 지문분류 방법은 일반적으로 특징추출과 분류 단계로 나누어진 두 단계의 과정을 하나로 통합하여 실행할 수 있다. 따라서 CNN 기반 방법은 지문 이미지의 특징을 자동으로 추출할 수 있으므로, 처리 과정을 단축시킬 수 있는 장점이 있다. 또한 불완전하거나 품질이 낮은 지문의 특징을 다양하게 학습할 수 있으므로, 예외 상황의 특징 추출에 대해 유연성이 있다. 본 논문에서는 CNN 기반 지문분류연구동향을 파악하고, 실험 방법 및 결과 분석을 통해 향후 연구방향에 대해 논의하고자 한다.