• 제목/요약/키워드: two-hybrid analysis

검색결과 762건 처리시간 0.024초

Two scale seismic analysis of masonry infill concrete frames through hybrid simulation

  • Cesar Paniagua Lovera;Gustavo Ayala Milian
    • Earthquakes and Structures
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    • 제24권6호
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    • pp.393-404
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    • 2023
  • This paper presents the application of hybrid-simulation-based adapter elements for the non-linear two-scale analysis of reinforced concrete frames with masonry infills under seismic-like demands. The approach provides communication and distribution of the computations carried out by two or more remote or locally distributed numerical models connected through the OpenFresco Framework. The modeling consists of a global analysis formed by macro-elements to represent frames and walls, and to reduce global degrees of freedom, portions of the structure that require advanced analysis are substituted by experimental elements and dimensional couplings acting as interfaces with their respective sub-assemblies. The local sub-assemblies are modeled by solid finite elements where the non-linear behavior of concrete matrix and masonry infill adopt a continuum damage representation and the reinforcement steel a discrete one, the conditions at interfaces between concrete and masonry are considered through a contact model. The methodology is illustrated through the analysis of a frame-wall system subjected to lateral loads comparing the results of using macro-elements, finite element model and experimental observations. Finally, to further assess and validate the methodology proposed, the paper presents the pushover analysis of two more complex structures applying both modeling scales to obtain their corresponding capacity curves.

Characterization of Burkholderia glumae Putative Virulence Factor 11 (PVF11) via Yeast Two-Hybrid Interaction and Phenotypic Analysis

  • Kim, Juyun;Kim, Namgyu;Mannaa, Mohamed;Lee, Hyun-Hee;Jeon, Jong-Seong;Seo, Young-Su
    • The Plant Pathology Journal
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    • 제35권3호
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    • pp.280-286
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    • 2019
  • In this study, PVF11 was selected among 20 candidate pathogenesis-related genes in Burkholderia glumae based on its effect on virulence to rice. PVF11 was found to interact with several plant defense-related WRKY proteins as evidenced through yeast-two hybrid analysis (Y2H). Moreover, PVF11 showed interactions with abiotic and biotic stress response-related rice proteins, as shown by genome-wide Y2H screening employing PVF11 and a cDNA library from B. glumae-infected rice. To confirm the effect of PVF11 on B. glumae virulence, in planta assays were conducted at different stages of rice growth. As a result, a PVF11-defective mutant showed reduced virulence in rice seedlings and stems but not in rice panicles, indicating that PVF11 involvement in B. glumae virulence in rice is stage-dependent.

Identification of a Domain in Yeast Chitin Synthase 3 Interacting with Chitin Synthase 4 by Two-Hybrid Analysis

  • Park, Hyun-Sook;Shin-Jung-Choi;Nok-Hyun-Park;Chi-Hwa-Kim;Sung-Uk-Kim
    • Journal of Microbiology and Biotechnology
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    • 제12권6호
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    • pp.943-949
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    • 2002
  • It has been proposed that chitin synthase 3 (CHS3)-nediated chitin synthesis during the vegetative cell cycle is regulated by chitin synthase 4 (CHS4) of Saccharomyces cerevisiae. To investigate direct protein-protein interaction between the coding products of these two genes, a domain of Chs3p that is responsible for interaction with Chs4p was identified, using the yeast two-hybrid system. This domain of 54 amino acids, termed MIRC3-4 (Maximum Interacting Region of Chs3p with Chs4p), is well conserved among CHS3 homologs of various fungi. Some mutations in MIRC3-4 resulted in a decrease in the enzymatic activity and chitin contents. Chs3p carrying those mutations exhibited weak interactions with Chs4p, when assayed by the yeast two-hybrid system. Surprisingly, all the mutants were sensitive to Calcofluor regardless of changes in enzymatic activities or chitin contents. This report deals with a core region in MIRC3-4 that affects the interaction with Chs4p.

병렬 하이브리드 전기자동차의 주요 구성시스템에 대한 상대적 가격 모델링 (Relative Cost Modeling for Main Component Systems fo Parallel Hybrid Electric Vehicle)

  • 김필수;김용
    • 대한전기학회논문지:전기기기및에너지변환시스템부문B
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    • 제48권6호
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    • pp.294-300
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    • 1999
  • There is a growing interest in hybrid electric vehicles due to environmental concerns. Recent efforts are directed toward developing an improved main component systems for the hybrid electric vehicle applications. Soon after the introduction of electric starter for internal combustion engine early this century, despite being energy efficient and nonpolluting, electric vehicle lost the battle completly to internal combustion engine due to its limited range and inferior performance. Hybrid Electric vehicles offer the most promising solutions to reduce the emission of vehicles. This paper describes a method for cost reduction estimation of parallel hybrid electric vehicle. We used a cost reduction structure that consisted of five major subsystems (three-type and two-type motor) for parallel hybrid electric vehicle. Especially, we estimated the potential for cost reductions in parallel hybrid electric vehicle as a function of time using the learning curve. Also, we estimated the potentials of cost by depreciation.

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Prediction of the long-term deformation of high rockfill geostructures using a hybrid back-analysis method

  • Ming Xu;Dehai Jin
    • Geomechanics and Engineering
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    • 제36권1호
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    • pp.83-97
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    • 2024
  • It is important to make reasonable prediction about the long-term deformation of high rockfill geostructures. However, the deformation is usually underestimated using the rockfill parameters obtained from laboratory tests due to different size effects, which make it necessary to identify parameters from in-situ monitoring data. This paper proposes a novel hybrid back-analysis method with a modified objective function defined for the time-dependent back-analysis problem. The method consists of two stages. In the first stage, an improved weighted average method is proposed to quickly narrow the search region; while in the second stage, an adaptive response surface method is proposed to iteratively search for the satisfactory solution, with a technique that can adaptively consider the translation, contraction or expansion of the exploration region. The accuracy and computational efficiency of the proposed hybrid back-analysis method is demonstrated by back-analyzing the long-term deformation of two high embankments constructed for airport runways, with the rockfills being modeled by a rheological model considering the influence of stress states on the creep behavior.

One-node and two-node hybrid coarse-mesh finite difference algorithm for efficient pin-by-pin core calculation

  • Song, Seongho;Yu, Hwanyeal;Kim, Yonghee
    • Nuclear Engineering and Technology
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    • 제50권3호
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    • pp.327-339
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    • 2018
  • This article presents a new global-local hybrid coarse-mesh finite difference (HCMFD) method for efficient parallel calculation of pin-by-pin heterogeneous core analysis. In the HCMFD method, the one-node coarse-mesh finite difference (CMFD) scheme is combined with a nodal expansion method (NEM)-based two-node CMFD method in a nonlinear way. In the global-local HCMFD algorithm, the global problem is a coarse-mesh eigenvalue problem, whereas the local problems are fixed source problems with boundary conditions of incoming partial current, and they can be solved in parallel. The global problem is formulated by one-node CMFD, in which two correction factors on an interface are introduced to preserve both the surface-average flux and the net current. Meanwhile, for accurate and efficient pin-wise core analysis, the local problem is solved by the conventional NEM-based two-node CMFD method. We investigated the numerical characteristics of the HCMFD method for a few benchmark problems and compared them with the conventional two-node NEM-based CMFD algorithm. In this study, the HCMFD algorithm was also parallelized with the OpenMP parallel interface, and its numerical performances were evaluated for several benchmarks.

Parametric analysis of hybrid outrigger system under wind and seismic loads

  • Neethu Elizabeth Johna;Kiran Kamath
    • Structural Engineering and Mechanics
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    • 제86권4호
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    • pp.503-518
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    • 2023
  • In tall constructions, the outriggers are regarded as a structural part capable of effectively resisting lateral loads. This study analyses the efficacy of hybrid outrigger system in high rise RCC building for various structural parameters identified. For variations in α, which is defined as the ratio of the relative flexural stiffness of the core to the axial rigidity of the column, static and dynamic analyses of hybrid outrigger system having a virtual and a conventional outrigger at two distinct levels were conducted in the present study. An investigation on the optimal outrigger position was performed by taking the results from absolute maximum inter storey drift ratio (ISDmax), roof acceleration (accroof), roof displacement (disproof), and base bending moment under both wind and seismic loads on analytical models having 40, 60 and 80 storeys. An ideal performance index parameter was introduced and was utilized to obtain the optimal position of the hybrid outrigger system considering the combined response of ISDmax, accroof, disproof and, criteria required for the structure under wind and seismic loads. According to the behavioural study, increasing the column area and outrigger arm length will maximise the performance of the hybrid outrigger system. The analysis results are summarized in a flowchart which provides the optimal positions obtained for each dependent parameter and based on ideal performance index which can be used to make initial suggestions for installing a hybrid outrigger system.

역복사 해석을 위한 혼합형 유전알고리즘에 관한 연구 (A Hybrid Genetic Algorithms for Inverse Radiation Analysis)

  • 김기완;백승욱;김만영
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2003년도 춘계학술대회
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    • pp.1639-1644
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    • 2003
  • A hybrid genetic algorithm is developed for estimating the wall emissivities for an absorbing, emitting, and scattering media in a two-dimensional irregular geometry with diffusely emitting and reflecting opaque boundaries by minimizing an objective function, which is expressed by the sum of square errors between estimated and measured temperatures at only four data positions. The finite-volume method was employed to solve the radiative transfer equation for a two-dimensional irregular geometry. The results show that a developed hybrid genetic algorithms reduce the effect of genetic parameters on the performance of genetic algorithm and that the wall emissivities are estimated accurately without measurement errors.

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혼종 모형을 이용한 간호 학습전이의 개념 분석 (A Concept Analysis on Learning Transfer in Nursing Using the Hybrid Model)

  • 손해경;김효진;김동희
    • 한국직업건강간호학회지
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    • 제29권4호
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    • pp.354-362
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    • 2020
  • Purpose: This study aimed to define and clarify learning transfer in nursing. Methods: This study used a hybrid model to analyze the concept of learning transfer in nursing through three phases. For the theoretical phase, learning transfer attributes were identified through a scoping literature review. In the fieldwork phase, in-depth focus group interviews were conducted to develop attributes. Purposive sampling was performed with ten participants(five nursing students, two nurses, three nursing faculty members). In the analysis phase, the attributes and final analysis of learning transfer in nursing were extracted and integrated from the previous two phases. Results: According to the analysis, learning transfer was represented in two dimensions with eight attributes. The development of competency dimension had three attributes: 1) theory acquisition, nursing skills, professional attitude, 2) integration, and 3) analysis competency. The competency change dimension had five attributes: 1) appropriateness in patient care, 2) proficiency in patient care, 3) satisfaction, 4) achievement, and 5) confidence. Conclusion: The concept analysis might provide a basic understanding of learning transfer, a development framework toward a measurement of nursing learning transfer and effective educational nursing strategies.