• Archives of Pharmacal Research
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• 제21권3호
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• pp.291-297
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• 1998

Cop protein has been overexpressed in Escherichia coli using a T7 RNA polymerase system. Purification to apparent homogeneity was achieved by the sequential chromatography on ion exchange, affinity chromatography, and reverse phase high performance liquid chromatography system. The molecular weight of the purified Cop was estimated as 6.1 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). But the molecular mass of the native state Cop was shown to be 19 kDa by an analytical high performance size exclusion chromatography, suggesting a trimer-like structure in 50 mM Tris-HCI buffer (pH 7.5) containing 100 mM NaCl. Cop protein Was calculated to contain $39.1% {\alpha}-helix, 16.8% {\beta}-sheet$, 17.4% turn, and 26.8% random structure. The DNA binding property of Cop protein expressed in E. coli Was preserved during the expression and purification process. The isoelectric point of Cop was determined to be 9.0. The results of amino acid composition analysis and N-terminal amino acid sequencing of Cop showed that it has the same amino acid composition and N-terminal amino acid sequence as those deduced from its DNA sequence analysis, except for the partial removal of N-terminal methionine residue by methionyl-aminopeptidase in E. coli.

• 천문학회보
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• 제38권1호
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• pp.41.2-41.2
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• 2013

We present the statistical properties of a volume-limited sample of 7,429 nearby (z = 0.033-0.044) galaxies from the Sloan Digital Sky Survey Data Release 7. By performing a visual inspection, we classified our sample galaxies according to the Hubble sequence (Hubble 1926, 1936). Then we excluded apparently smaller and flatter galaxies from our database because morphology classification on them turned out to be difficult. Our results cover structural (e.g. concentration index, color, magnitude, stellar mass, etc.), spectroscopic (e.g. velocity dispersion, $H{\beta}$ absorption line, Fe absorption line, Mg absorption line, accretion rate, $H{\alpha}$ emission line, etc.), and environmental (e.g. density, etc.) properties of each morphology type based on morphology distribution. For this analysis, we used the recent re-measurements of spectral line strengths by Oh and collaborators (2011). Our statistics confirm the up-to-date understanding on galaxy populations, e.g., correlations between morphology and line strengths and in turn derived ages and so on.

• Bulletin of the Korean Chemical Society
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• 제19권1호
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• pp.57-62
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• 1998

We have synthesized a 17-residue peptide with the amino acid sequence RQMKEDAKGKSEEELAD corresponding to residues 84-100 of chicken skeletal troponin C. This stretch of the protein sequence is in the middle one-third of the 32-residue 9-turn α-helix that connects the two globular domains of the dumbell-shaped molecule and includes the D/E linker helix. We describe here the solution conformation of the helix linker as studied by circular dichroism (CD) and two-dimensional nuclear magnetic resonance (2-D NMR) spectroscopy. The NOE connectivities together with the vicinal $^3J_{N{\alpha}}$ coupling constants suggest that the peptide exists in a fast conformational equilibrium among several secondary structure: a nascent helix near the N-terminus, a helix, and a substational population of extended and random coil forms. In addition, two interresidue α-α NOEs are observed suggesting a bent structure with a bend that includes the single glycine in position 92. These results are consistent with the ideas that in neutral solution the D/E linker region of the central helix in troponin C can adopt a helical conformation and the central helix may have a segmental flexibility around Gly 92.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.330.2-330.2
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• 2002

The conformational study on cyclic Ac-Cys-Pro-Xaa-Cys-NHMe (Ac-CPXC-NHMe: X = Ala, Val. Leu. Aib. Gly. His. Phe, Tyr. Asn. and Ser) peptides has been carried out using the ECEPP/3 force field and the hydration shell model in the unhydrated and hydrated states. This work has been undertaken to investigate structural implications of the CPXC sequence as the chain reversal for the initiation of protein folding and as the motif for active site of disulfide oxidoreductases. The backbone conformation DAAA is in common the most feasible for cyclic CPXC peptides in the hydrated state. which has a type 1${\beta}$-turn at the Pro-Xaa sequence. The proline residue and the hydrogen bond between backbones of two cystines appear to play a role in stabilizing this preferred conformation of cycilc CPXC peptides. However. the distributions of backbone conformations and ${\beta}$-turns may indicate that the cyclic CPXC peptide seems to exist as an ensemble of ${\beta}$-turns and coiled conformations. The intirnsic stability of the cyclic CPXC motif itself the active conformation appears to play a role in determining electrochemical properties of disulfide oxidoreductases.

• Journal of Platform Technology
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• 제6권1호
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• pp.17-23
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• 2018

승강장 진입을 위한 택시 대기열은 택시 승강장 주변에 교통 정체를 일으켜 주변을 혼잡하게 만든다. 이러한 정체와 혼잡은 택시 승강장 주변이 아닌 다른 곳에 택시들이 대기하도록 하고 승강장에 진입이 가능한 택시만 택시 승강장에 접근하도록 함으로써 감소시킬 수 있다. 이를 위해 본 논문에서는 택시 승강장 진입 순서 제어를 위한 승강장 진입 순번 운영 시스템을 제안한다. 이 시스템은 택시 기사가 선택한 승강장 진입 순번 신청을 수신하면 그 승강장의 상태에 따라 적절한 순번을 할당하며, 그 승강장에 진입할 순서가 되면 택시 단말기를 통해 승강장 진입 순서가 되었음을 공지할 수 있다. 이 서비스를 이용하는 택시는 택시 승강장 진입을 위한 대기열에 있지 않고 다른 곳에서 대기를 하며, 승강장 진입 알림을 받은 후 택시 승강장에 접근하면 된다. 따라서, 이 시스템은 택시 승강장 주변에 택시 대기열을 감소시키거나 제거할 수 있어 승강 주변의 교통 정체 및 혼잡을 감소시킬 수 있다. 본 논문에서 제안한 승강장 진입 순번 운용 시스템은 충청북도 충주시에 설치되어 있는 택시 승강장을 대상으로 하여 구글의 클라우드 서비스와 안드로이드 플랫폼을 이용하여 구현하였다.

• BMB Reports
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• 제32권5호
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• pp.461-467
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• 1999

Recombinant Mycobacterium smegmatis expressing ovalbumin was used to immunize C57BL/6(H-$2^b$) mice, and the humoral immunity against recombinant ovalbumin was analyzed. Antibodies were purified by denatured ovalbumin-conjugated affinity chromatography. The epitopes of the antibodies were screened with a random peptide library displayed on the tip of fUSE5 filamentous phage pIII minor coat proteins. Two peptides, IRLADR and SPGAEV, were selected predominantly by the recognition of purified antibodies using biopanning methods. The composition of the peptide sequence with the primary structure of OVA revealed that the peptide sequence analogizes to INEAGR, part of the $^{323}ISQAVHAAHAEINEAGR^{339}$ sequence previously reported as the antigenic determinant for murine Band also Th cell epitopes (I-$A^d$ binding). Also, the structures of these mimotopes obtained from restrained molecular dynamic computations resulted in the formation of a $\beta$-turn proven to be a secondary structure of the parent peptide within the ovalbumin molecule, enabling us to confirm the structural similarity. This study demonstrates that immunization with recombinant M. smegmatis can generate neutralizing antibodies identical with those induced by the administration of natural antigenic proteins and supports the potential use of mycobacteria as vaccine delivery vehicles.

• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.749-755
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• 2005

The ccpA gene encoding catabolite control protein A (CcpA) of Leuconostoc mesenteroides SYl, a strain isolated from kimchi, was cloned, sequenced, analyzed for transcript, and overexpressed in Escherichia coli. The ccpA ORF (open reading frame) is 1,011 bp in size, which can encode a protein of 336 amino acid residues with a molecular mass of 36,739 Da. The transcription start site was mapped at a position 49 nucleotides upstream of the start codon, and promoter sequences were also identified. The putative cre site overlapped with the -35 promoter sequence. The deduced amino acid sequence of the CcpA contained the helix-turn-helix motif found in many DNA-binding regulatory proteins. CcpA from 1. mesenteroides SY1 had $54.6\%$ identity with CcpA from Lactobacillus casei. The Northern blot experiment showed that ccpA was transcribed as a single 1.1 kb transcript, and transcription was repressed when grown on media containing glucose. CcpA was overproduced in E. coli BL21(DE3) cells using the pET expression vector, and purified to an apparent homogeneity. Gel Mobility Shift Assay with purified CcpA and a DNA fragment containing the ere sequence of the $\alpha$-galactosidase gene (aga) from L. mesenteroides SY1 revealed that CcpA bound specifically to the cre site of aga.

• 조형예술학연구
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• 제2권
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• pp.239-293
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• 2000

The paper aims to study how to describe the rigorous position on which semantic elements are laid, within the visual space. This purpose is, first of all, for the construction of visual syntactical semantics of the visual space. For this aim, the preliminary research begins with some definitions on : 1) visual space, visual design, visual syntactical semantic meaning, 2) position, sequence of position, 2-dim. sequence of position, and 3) 2-dim. numerical series of position, harmonious dynamic values. Here, The main issue is to define the position in general. To solve this problem, the researcher surveyed the positions on which the sets of pixemes are set up. The results are as follows ; 1. As far as the positions in visual space are concerned, they are the possible locations permitted in the visual patches. They are not the factual but the possible positions. 2.The position value that the sequential point has is not arithmetical but harmonious-dynamic, for it is not permitted for its own sake, but for the person, that is to say, for the viewer's visual perception. 3. The harmonious-dynamic value of positional sequence can be composed of the 2-dimensional successive numerical series which is, in turn, composed of the primordial 3 values x0, $\mu0$, y0. Here, the $\mu0$ is the harmonious mean value of x0 and y0. The x0 and y0 are, therefore, of the mutual dynamic relationship. 4. From this, the 2-dim general $\Delta-TABLE$ of the numerical series of position in visual design could be acquired through development of the primordial 3 values into the $X_i,\;Y_i$, orbit values.

• Journal of Microbiology and Biotechnology
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• 제9권4호
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• pp.457-463
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• 1999

The physical map of bacteriophage $\PhiFC1$ DNA was constructed with the restriction endonucleases SalI, BamHI, EcoRI, XbaI, and AvaI. The 40.5-kb DNA restriction map is shown to be circularly permuted representing the headful packaging mechanism of the phage. The DNA restriction fragments containing the packaging initiation site(pac) was localized on the restriction map and the nucleotide sequences of the region were analyzed. Four open reading frames (ORFs), following one another with the same orientation, were found at the region. The 2nd ORF (ORF-ts) has significant amino acid sequence homologies to the previously known terminase small subunits of other bacteriophages. The putative terminase small subunit gene has a presumptive NTP-hydrolysis motif and a helix-turn-helix motif. The cleavage site for the first round of packaging was found to be located at the coding sequence of the putative terminase small subunit gene. The fourth ORF, even if partially sequenced, has a good amino acid sequence homology to the portal vertex proteins of other bacteriophages representing the evolutionarily conserved arrangements of genes near the pac site of this bacteriophage, $\PhiFC1$.

• Nuclear Engineering and Technology
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• 제54권6호
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• pp.2135-2146
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• 2022

A nuclear energy facility is one of the most critical facilities to be safely protected during and after operation because the physical destruction of its barriers by an external attack could release radioactivity into the environment and can cause harmful effects. The barrier walls of nuclear energy facilities should be sufficiently robust to protect essential facilities from external attack or sabotage. Physical protection system (PPS) vulnerability assessment of a typical small nuclear research reactor was carried out by simulating an external attack with a tri-nitro toluene (TNT) shaped charge and results are presented. The reinforced concrete (RC) barrier wall of the research reactor located at a distance of 50 m from a TNT-shaped charge was the target of external attack. For the purpose of the impact assessment of the RC barrier wall, a finite element method (FEM) is utilized to simulate the destruction condition. The study results showed that a hole-size of diameter 342 mm at the front side and 364 mm at the back side was created on the RC barrier wall as a result of a 143.35 kg TNT-shaped charge. This aperture would be large enough to let at least one person can pass through at a time. For the purpose of the PPS vulnerability assessment, an Estimate of Adversary Sequence Interruption (EASI) model was used, which enabled the determination of most vulnerable path to the target with a probability of interruption equal to 0.43. The study showed that the RC barrier wall is vulnerable to a TNT-shaped charge impact, which could in turn reduce the effectiveness of the PPS.