• Title/Summary/Keyword: tuber crop

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Cloning and Characterization of GDP-mannose Pyrophosphorylase from Solanum Tuberosum L.

  • Hyun, Tae-Kyung;Lim, Jung-Dae;Kim, Jae-Kwang;Seong, Eun-Soo;Lee, Jae-Geun;Yoon, Byeong-Sung;Kim, Myong-Jo;Cho, Dong-Ha;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.5
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    • pp.276-283
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    • 2005
  • Ascorbic acid is a great antioxidant and helps protect the body against pollutants. GDP-mannose pyrophosphorylase (GMPase) is a key enzyme in manufacturing GDP-mannose, a glycosyl donor for ascorbate and cell wall biosynthesis as well as for protein glycosylation. In this study, we described molecular cloning of a full-length cDNA from Potato (Solanum tuberosum L. cv. Jasim), using tuber. The cDNA isolated encoded a GDP-mannose pyrophosphrylase. The nucleotide sequence of pGMPC showed about 95%, 89% and 80% homology with S. tuberosum (AF022716), N. tabacum (AB066279) and A. thaliana (AF076484) cDNAs clone known as GMPase, respectively. We detected the expression of GMPase using RT-PCR. The highest expression of GMPase was found in stems, and the largest amount of ascorbic acid was also presented in stems. In contrast, the leaf showed minimal level of GMPase transcript and ascorbic acid content. We propose that GMPase expression patterns were similar to the changes of ascorbic acid content in the leaves treated with diverse stresses.

Changes of Endogenous Gibberellins in Tubers of Chinese Yam(Dioscorea opposita) during Storage Period

  • Lee, In-Jung;Kim, Sang-Kuk;Lee, Sang-Chul;Lee, Bong-Ho;Jeong, Hyung-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.4
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    • pp.281-285
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    • 2003
  • For storage periods of tubers in Chinese yam, the levels of $\textrm{GA}_{44}$ and $\textrm{GA}_{20}$ was constant, meanwhile both $\textrm{GA}_{53}$ and $\textrm{GA}_{19}$ level were always higher than that of $\textrm{GA}_{44}$ and $\textrm{GA}_{20}$$\textrm{GA}_9$ content as precursor of $\textrm{GA}_4$ was not changed during storage. $\textrm{GA}_{24}$ content was low to below 0.2 ng for 90 days after storage, $\textrm{GA}_{36}$ content as precursor of $\textrm{GA}_4$ like $\textrm{GA}_9$ was about 6-8 fold higher than that of $\textrm{GA}_9$ during storage. GA contents of the two gibberellin biosynthetic pathways were gradually increased when storage periods were progressed. Bioactive GA$_1$ content as the GA members of an early C-13 hydroxylation was always constant, and its content was very low as below 0.1ng per dry weight, meanwhile, bioactive $\textrm{GA}_4$ content as the GA members of non C-13 hydroxylation was drastically increased, also, its content was highest at 90 days after storage, and then decreased at 120 days after storage. Consequently, we suggest that $\textrm{GA}_4$ may be involved in controlling tuber sprouting in Chinese yam.

Control of Perennial Weed Scirpus maritimus L. in Reclaimed Paddy Fields of West Seashore II. Physiological and Ecological Characteristics of S. maritimus (서해안 간척답에 있어서 다년생잡초매자기 방제에 관한 연구 제 2 보 매자기의 생리생태적 특성)

  • 양환승;전재철;문영희
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.23 no.1
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    • pp.64-73
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    • 1978
  • To gain a better understanding of the germination and growth of Scirpus maritimus, pot and field experiments were conducted to observe the development of new rhizomes and tubers, the growth rate of culms. and the germination behaviour of tubers of this perennial weed. Tuber placement, water depth, salinity and pH were also observed for their possible influence on the growth of S. maritimus.

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Cultural Environments on Growth and Tuberlet Yield of Pinellia ternata(Thunb.) Breit (반하의 재배환경에 따른 생육 및 수량)

  • Kim, Young-Jin;Park, Moon-Soo;Park, Ho-Ki;Kim, Sun;Kim, Tai-Soo;Chang, Young-Sun
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.3
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    • pp.240-245
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    • 1995
  • This study was conducted to find out environment conditions in order to increase of growth and yield of tuberlets in pinellia ternata (Thunb.) Breit. The optimal temperature conditions for the growth of tuberlets were good rather temperature fluctuations than constant one and the optimal photoperiod was a 6hr-photoperiod. The growth of tuberlets to the kinds and concentration of growth regulators was good for 0. lppm 2.4- D, 10ppm kinetin and 50ppm gibberellin treatment. In colchicine soaking treatment, the growth of tuberlets was bad, but bulblets formation was good for 0. 05% colchicine treatment for 48hr. -soaking. In colchicine dropping treatment, dry tuber yield was good for 0. 1% colchicine treatment with once dropping for one day.

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Inhibitory Effects of Some Treatments on Browning During Yam(Discorea batatas Decne) Tuber Processing (가공과정중(加工過程中) 마 괴근조직(塊根組織)의 갈변억제처리(褐變抑制處理) 효과(效果))

  • Kang, Dong-Kyoon;Kim, Sang-Kuk;Chung, Sang-Hwan;Lee, Seong-Phil;Choi, Boo-Sull
    • Korean Journal of Medicinal Crop Science
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    • v.6 no.1
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    • pp.33-37
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    • 1998
  • Browning index rised rapidly within 15 hrs to 83% of total browning in Jang - ma (Dioscorea batatas Decne) tissues at $40^{\circ}C$ while 37% in Dan - ma. Therefore, the degree of browning process of Jang - ma was faster than that of Dan - ma. Browning in Jang - ma was inhibited by treatments of NaCl which showed 48 and 39% inhibition, respectively, after 48 hrs of incubation. Also the treatment of $80^{\circ}C$ heating showed excellent inhibition within 24 hrs. In the case of Dan - ma, browning process was inhibited by treatments of 1 and 0.5M NaCl and $70^{\circ}C$ heating, which showed 43, 26 and 23% inhibition, respectively, after 48 hrs of incubation.

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Effects of Nitrogen and Potassium on Plant Growth, Yield and Nodule Formation in Winged Bean(Psophocarpus tetragonolobus(L.) D. C.) (질소와 가리시용이 Winged bean의 생육, 수량 및 근류형성에 미치는 영향)

  • 손상목;이장석
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.6
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    • pp.525-531
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    • 1990
  • This study was conducted to find out the effects of nitrogen, potassium application on the plant growth, grain yield and nodule formation of Winged bean. Plant height, number of branches, number of leaves, leaf area, NAR, RGR and CGR increased with basal and top dressing of nitrogen and potassium application. Especially CGR was increased significantly with the added amount of potassium. Number of nodules and nodule dry weight decreased with application of basal and top dressing nitrogen, but increased with the added amount of potassium. Green pod, tuber and grain yield increased with application of basal and top dressing nitrogen, and the added amount of potassium. There appeared positive correlation coefficient between grain yield and growth charateristics, and between grain yield and components. But there showed negative correlation coefficient among yield, number of nodules, dry weight and P$_2$O$\_$5/ contents in leaf.

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Effect of Recycled Paper Mulch on Yield of Fall-grown Potato

  • Lee, Byun-Woo;Cui, Ri-Xian;Lee, Suk-Ha
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.1
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    • pp.68-73
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    • 2002
  • High temperature during sprout emergence period of potato (Solanum tuberosum L.) is a major limitation to the yield of fall-grown potato in Korea. To explore the possibility of improving the yield of fall-grown potato through recycled paper mulching, the changes of soil environment and the growth and yield of potato cv. Daeji as affected by three mulching treatments of non-mulched control, recycled paper mulching (RPM), and black polyethylene film-mulching (BPFM) were examined over two fall seasons at Suwon, Korea. The mulching materials were a recycled mulch paper with 110 g/$m^2$ and a thickness of 0.1 mm, which was manufactured from old corrugated containers for this experiment and a commercial black polyethylene film with a thickness of 0.01mm. On an average throughout the growing period, the soil temperature with RPM at the 5-cm depth was lower by 0.4-1.1$^{\circ}C$ than that of the control. The maximum temperature during daytime in RPM was lowered by approximately 1 to 5$^{\circ}C$ according to the weather condition during emergence period, the difference being great on a fine day. The soil temperature with BPFM was much higher than the control. The effect of the mulching treatments diminished as the canopy became developed. The mulching treatments were more advantageous than the control in the conservation of soil water. Moreover, RPM and BPFM efficiently suppressed the occurrence of weeds until the potato harvest. RPM improved the emergence significantly due to lower soil temperature, whereas BPFM showed much poorer emergence than the control. Growth after emergence and yield were significantly higher in RPM than in the control and BPFM. It was concluded that the significantly improved yield in RPM compared to that in the control was mainly due to the improvement of the sprout emergence and tuber growth accompanied by lower soil temperature and better conservation of soil water.

Genetics, Agronomic, and Molecular Study of Leaflet Mutants in Mungbean(Vigna radiata(L.) Wilczek)

  • Soehendi, Rudy;Chanprame, Sontichai;Toojinda, Theerayut;Ngampongsai, Sumana;Srinives, Peerasak
    • Journal of Crop Science and Biotechnology
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    • v.10 no.3
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    • pp.193-200
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    • 2007
  • Mungbean plants generally have a relatively close canopy, thus a large amount of self-shading can reduce yield due to poor light penetration. Modification of leaflet type can affect leaf canopy and could alter seed yield. Two multiple leaflet mutants were obtained from gamma-ray irradiation and used to study the mode of inheritance related to leaflet types and to evaluate their agronomic features. The cross between large-heptafoliate leaflet with small-pentafoliate leaflet mutants produce all $F_1$ plants with normal trifoliate leaflets. The $F_2$ plants segregated in leaflet size and leaflet number into a 9:3:3:1 ratio of large-trifoliate: large-heptafoliate: small-pentafoliate: small-heptafoliate plants, suggesting that independent loci control leaflet size and leaflet number. Regarding leaflet number, the $F_2$ population can be classified into normal-trifoliate, small-pentafoliate, large-heptafoliate, and small-heptafoliate at the dihybrid ratio of 9:3:3:1. The gene symbols $N_1,n_1$ and $N_2,n_2$ are proposed to represent leaflet number. Since no plant was found with large-pentafoliate leaflets, we hypothesize that the $N_2$ allele expresses pleiotropic effect on both leaflet number and leaflet size. Another possibility is that an additional locus with S and s alleles controls leaflet size and S is tightly linked with $N_2$. The effect of multifoliate leaflet on yield and yield components was evaluated in four mungbean families each with four leaflet isolines under three environments. Averaging across the families and environments, the normal-trifoliate and large-heptafoliate lines gave higher yield than small pentafoliate and heptafoliate ones. These two large leaflet lines also had higher leaf area per plant than the other multifoliate lines. Therefore, the mungbean lines with a greater leaf area, which were likely to intercept more sunlight, gave greater yield. Three AFLP markers that were found to be linked to number of leaflets per leaf, corresponded to the N1 allele of the smallpentafoliate parent.

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Studies on storage of potato chip variefies on spring crop (춘작 재배시 Chip 가공용 감자 품종에 따른 저장성 연구)

  • Kim, Kyung-Je;Lee, Eun-Sang
    • Korean Journal of Organic Agriculture
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    • v.10 no.4
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    • pp.69-78
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    • 2002
  • This experiment was conducted to investigate the changes of sugar contents and chip color during 104days storage after harvesting of five potato varieties. The potato varieties were planted on 1st April in 1999 and harvested on 10. July in 1999. $No_2$ contents in potato petiole tended to decrease repidly at tuber maturing stage. $K^+$ contents in potato petiole tended to in crease at 70 days ofter planting on medium maturing varieties, and at 90 days after planting on late maturing variety. Snowden variety was no desirable cultivar for processing on spring cultivation due to long growth period. Contents of solid and sugar in potatoes affected on potato chip color. Higher contents of solid in potato varieties showed low sugar contents and no change on chip color during storage.

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Influence of Fertilization Treatment using Organic Amendment based on Soil Testing on Plant Growth and Nutrient Use Efficiency in Potato (토양검정에 의한 유기자원 시비처방이 감자의 생육 및 양분이용효율에 미치는 영향)

  • Lim, Jin-Soo;Lee, Bang-Hyun;Kang, Seung-Hee;Lee, Tae-Guen
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.436-446
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    • 2020
  • In this study, we verified the effects of organic amendment application based on soil testing in potato cultivation. The application rate of organic amendments was determined based on the inorganicization rate of nitrogen, phosphoric acid, and potassium in the organic amendments. There was no significant difference in the length, stalk number, stalk diameter, and leaf color of potato plants under organic amendment application compared with those under chemical fertilization. The quantity of potato tubers and yield of marketable tubers under organic amendment application were higher than those under chemical fertilization. The top fresh weight and top-to-root ratio were the highest under organic amendment application, followed by those under chemical fertilization and the control. The inorganic nitrogen content in the leaves and stems of potato plants was the highest under chemical fertilization. There was no difference in the phosphoric acid content between the potatoes under chemical fertilization and those under organic amendment application. The potassium content in potatoes was higher under chemical fertilization than that under organic amendment application. While the change trend of inorganic nitrogen content in the roots was similar to that in the leaves and stems, the potatoes under organic amendment application, with the highest yield, showed the highest dry weight. The tuber weight showed a positive correlation with plant length, stalk number, and stalk diameter. The higher the weight of the tuber, the higher the weight of the marketable tuber, and the higher the top fresh weight, the higher the yield. Therefore, to increase yield, it is necessary to supply nutrients to improve the top fresh weight to the optimal level. There was no difference in the utilization efficiency of nitrogen and potassium between the potatoes under chemical fertilization and those under organic amendment application. The utilization efficiency of phosphoric acid was increased by 0.1% in potatoes under organic amendment application compared with that under other treatments. Regarding soil chemistry after harvest, the soils under organic amendment application showed a higher electrical conductivity and higher nitrogen and phosphoric acid content than those under other treatments. Therefore, the organic amendment method used in this study can be an alternative to chemical fertilization. It is also advantageous for the cultivation of subsequent crops in potato fields.