• Journal of the Korean Society of Food Science and Nutrition
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• 제14권4호
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• pp.345-352
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• 1985

To characterize the protein from arrowroot leaf, proteins were extracted and separated from arrowroot leaf, then its amino acid composition and functional properties were studied. Protein in arrowroot leaf was consisted of 18.5% albumin, 33.5% globulin, 34.0% glutelin, 6.2% protamine and 7.8% insoluble residues. The rates of precipitation of proteins which extracted with water, 1M NaCl, and 0.015N NaOH as a solvent were 84.7% (at pH 3.0), 76.4% (at pH 2.5) and 86.4% (at pH 4.0), respectively. The extracted proteins were separated up to about 90% by organic solvents such as ethanol and acetone at 80% concentration, Composition of arrowroot leaf protein concentrates were: $1{\sim}2%$ moisture, $59{\sim}67%$ protein, $4{\sim}8%$ ash and $5{\sim}6%$ (dialyzed concentrates) or $1{\sim}2%$ (acetone-treated ones) lipid. Main amino acids of the concentrates were aspartic acid, glutamic acid and glycine. Solubility profile of the concentrates according to pH was typical. The minimum solubility (below pH 5.0) of acetone extracted protein concentrates was lower than that of unextracted ones, whereas the reverse was true for pH value above this region. Bulk density, water and fat absorption of the concentrates were attributable to correlation to the treatment of acetone. And the bulk density of the concentrates was negatively correlative to both water and fat absorption. Emulsifying and foaming properties were not varied with the treatment of acetone.

• The Journal of Natural Sciences
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• 제7권
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• pp.91-101
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• 1995

To establish the simple and practical test method of estimating resistance of rice varieties against rice leafblight, trials were made to correlate among the four test methods such as true resistance test,secondaryinfection test, secondary epidemic test and field test. The results obtained through the experiment on 25 ricevarieties and 3 pathogenic isolates designated to HB9O11, HB9022 and HB9033 can be summarized asfollows. 1 .Based on the results of true resistance test, 25 rice varieties can be classified into four groups:1 )Samgang variety group ;resistant to HB9011, 13 varieties, 2)Pungsan variety group ; resistant to HB9011 and HB9022, 5 varieties, 3)Samgang variety group ; resistant to all three isolates, 2 varieties, 4)Unbongvariety group: sensitive to all three isolates, 12 varieties. 2. The responses of rice varieties to isolates showed some discrepancies among on the test methods. These examples were found in 3 varieties including Yeongdeog varieties to HB9011, 3 varieties including Taebaeg variety to HB9022 and Taebaek vaieties to HB9033. 3. Correlation coefficiences between the secondary infection test and the secondary epidemic test for HB9011, HB9022 and HB9033 were 0.972, 0.894 and 0.919, respectively. It suggests that the two methods are not significantly different so that one of the two methods are not significantly different so that one of the two methods can be omitted from resistant test without affecting the result. 4. Between the true resistance test and the field test at the disease common area, there were no significant correlations. Unbong, Chucheong and Yeongdeog varieties are appeared as resistant varieties in the true resistant test, but their responses in the field test were different and appeared as sensitive varieties. 5. The disease index was used to express theresults from four test methods. The disease index was calculated as the sum of each numerical values of theresults from the four test methods by giving the same weights(0 to 25)to each test method. If the disease index for certain variety is less than 15, then the variety is considered to be resistant. 4 varieties such as Seohae, Hwajin, Yeongdeog and Pungsan varieties-disease indices were less than 15 were selected as field resistance varieties.

• Journal of Korean Society of Forest Science
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• 제42권1호
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• pp.1-38
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• 1979

The woody species of Genus Ilex which are endemic to Korea are distributed on limited area due to solely temperature factor. There is some differences according to species, however in general, the evergreen Ilex are found along southern coastal area of Korean Peninsula and near islands where the cold index does not exceed $-5^{\circ}C$. But Ilex macropoda and the variety, only deciduous ones, are grown in temperate zone of the peninsula and some islands. The list of Ilex species of Korea are as follows. Ilex cornuta Lindley et Pax., I. crenata Thunb. var. microphylla Max., I. crenata Thunb., I. rotunda Thunb., I. macropoda Miq., I. macropoda Miq. var. pseudo-macropoda Loensner, I. integra Thunb. The author surveyed the populations of Ilex species as many as possible and data of some characters such as leaf shape, spine, fruit shape, stomata density, sex ratio in natural communities, etc. are collected. Almost all the Ilex species in Korea show sporadic distribution. This means quite small sized populations isolate distantly each other eliminating the change of gene exchange in between. Particularly Ilex conuta and I. crenata show the morphological differentiation among populations as well as significant individual variation within a population. These were true with such characteristics, leaf shape, leaf dimension, leaf margin, fruit shape, spine, and stomata density. The founded are that the fruit length and the stomata density counted on the beneath surface of leaves of Ilex cornuta increased with the decrease of latitude. These are naturally closely related with the cold index values. The table shown below indicates the correlation between mean stomata density per $0.3642mm^2$ and cold index values. These relation however were not observed on Ilex crenata. The most dominated natured in relation to individual variation were outline of leaf, the number of marginal spine, the shape of leaf cross section and the degree of luster of the upper leaf surface. As shown in photos 5~7, these variations are agreed at a glance. There are reports that the development of marginal spines in some Ilex species is associated with the juvenility and topophysis. In present study, these two factors were neglected because of the intended sampling procedure. Of Ilex rotunda, population difference with the characteristics of leaf length is recognized but not for leaf width, petiole length, and fruit size. However, individual variations within a population were significantly large. In case of Ilex integra, only individual differences within population were calculated statistically for such characteristics as leaf length, leaf width, and petiole length. As to natural population, the sex ratio was 1:2 (female to male) for Ilex cornuta, and 1:1 for Ilex crenata. The tendency of more male than female in I. cornuta was agreed to other observations. Preparing the tip cutting of length 10cm, and treating with IBA, then attaching earth ball to the cut end, very successful rooting percentages were obtained. Asexual propagation has the advantages of maintaining the heterozygosity of existing varieties and overcoming the difficulties of delayed seed germination frequently encountered with Ilex species. Considering a great deal of variation in morphological traits, a good possibility of selection breeding for decorative and ornamental purposes exists. At present, these evergreen Ilex are ignored by local people as nuisance weedy shrubs. So the proper protection measures should promptly be taken.

• The Korean Journal of Ecology
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• 제22권3호
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• pp.119-129
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• 1999

The bryophyte communities on the forest floor of the mixed coniferous and deciduous broad-leaf forest and the evergreen coniferous forest of cool temperate and subalpine zones in Mts. Taebaek, Odae and Sulak of Tabaek Range were investigated from. the numerical and phytosociological viewpoints. As a result, four bryophyte communities were recognized there, considering the species composition of vascular vegetation; A. Thuja koraiensis-Abies nephrolepis/Taxus caespitosa-Pinus pumila/Hylocomium splendens-Pleurozium schreberi community, B: Sasamorpha borealis-Abies nephrolepis/Plagiomnium cuspidatum- Aulacomnium heterostichum community, C. Abies holophylla-Abies nephrolepis/Thuidium recognitum-Trachycystis immarginata cristatus community, D. Meehania urticifolia-Abies nephrolepis/Hylocomiopsis ovicarpa-Taxiphyllum aomoriense community. Among these, the community A was distributed in higher altitudes and characterized by the species group of the holarctic elements, e.g. Hylocomium splendens, Pleurozium schreberi, Dicranum majus, etc. limited in subarctic; subalpine and arctic; alpine zones and was confirmed as synusies of the Thujo-Abietetum nephrolepidis Song 1991 and Taxo-Pinetum pumilae Song 1985 belonging the Vaccinio-Piceetea Br.-Bl. 1939. In contrast the communities B, C and D were distributed in the mixed coniferous and deciduous broad-leaf forest of lower altitudes and characterized by the elements of the cool temperate; montane zone. They were confirmed as synusies belonging to the Corylo-Quercetum mongolicae Song 1988. By the result of the numerical syntaxonomical analysis such as cluster analysis, interspecific association, PCA, the communities were largely divided into the two types. The two types coincided with the community units or the diagnostic species of the bryophyte communities of the subalpine zone and the montane zone, respectively. Also the analysis revealed that the division of the above two forest zones are justifiable. Up to date many investigator have described the coniferous forest distibuted above 1000m in Taebaek mountains as the forest vegetation of subalpine zone. However, from the results of the present study, the boundary of the mixed forest and the true subalpine coniferous forest was elucidated to be ca. 1,500 m phytosociologically. Species richness was the highest in the community A, and the other four communities showed a similar species richness.

• Korean Journal of Medicinal Crop Science
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• 제13권6호
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• pp.268-272
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• 2005

This study was carried out to investigate the labor saving cultivation of C. auriculatum, which has much bigger root than local variety C. wilfordii, and tested three nonsupporting methods, i.e., untreated control, top pinching, and cutting of branch-top compared with I type stake. Top growth was more vigorous in staking cultivation than in nonsupporting cultivations. Among nonsupporting methods, main vine length and total length of main vines per plant were increased in order of untreated control > top pinching > cutting of branch-top, while the opposite is true to diameter of main vein. Number of leaves per plant was lower, but leaf length and leaf width were higher in cutting of branch-top than in other nonsupporting methods. Number of branched root and diameter of main root were bigger in cutting of branch-top and in staking cultivation, while length of main root and diameter of branched root were greater in staking cultivation and in top pinching than in other treatments. Although yield of marketable root per 10a was $17.9{\sim}22.6%$ lower, income was $8%{\sim}17%$ higher in nonsupporting methods than in staking cultivation by cost saving to staking installation. Among nonsupporting methods, yield of marketable root and income were 6% and 8% higher in cutting of branch-top than in untreated control, respectively.

• Horticultural Science & Technology
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• 제33권3호
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• pp.349-355
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• 2015

Lisianthus (Eustoma grandiflorum) is relatively sensitive to temperature and light conditions. For year round production of good quality potted plants and energy saving, it is necessary to understand the growth and flowering response to the combined conditions of these environmental factors. This study was conducted to examine the growth and flowering responses to temperature, photoperiod, and light intensity during the post-seedling stage. 'El Paso Deep Blue' lisianthus plants with four true leaf pairs were grown in growth chambers maintained at average daily temperatures (ADT) of 14, 20, and $26^{\circ}C$ and provided with three photosynthetic photon fluxes [PPF; 100, 200, and $400{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$] for 8 (08:00-16:00) and 16 hours (08:00-24:00) by fluorescent and incandescent lamps, resulting in four daily light integrals (DLI): about 3, 6 (two photoperiods), 12 (two photoperiods), and $24mol{\cdot}m^{-2}{\cdot}d^{-1}$. After treatment for three weeks followed by growth for one week in a greenhouse of $20{\pm}3^{\circ}C$, growth and development were measured. Higher temperature, higher PPF, and longer photoperiod promoted plant growth and flowering; however the impacts of PPF and photoperiod were smaller than those of temperature. As ADT and DLI increased, the number of leaves, number of flowers, lateral shoot length, and shoot dry weight increased. An increase of about $1mol{\cdot}m^{-2}{\cdot}d^{-1}$ DLI could constitute an increase of 0.40 to $0.76^{\circ}C$ ADT depending on these crop characteristics when ADT and DLI are above $20^{\circ}C$ and $12mol{\cdot}m^{-2}{\cdot}d^{-1}$, respectively. Therefore, growers can select a regimen of heating or supplemental lighting without delaying harvesting time or decreasing crop quality.

• Journal of the Korean Society of Tobacco Science
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• 제30권1호
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• pp.1-7
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• 2008

Bacterial wilt caused by Ralstonia solanacearum has become a severe problem on tobacco in Korea. No effective single control measure is available at present time. One of the most potential way for controlling the bacterial wilt on tobacco is growing tobacco cultivars resistant to the bacterial wilt. In this study, optimal conditions for screening tobacco cultivars resistant to the bacterial wilt were examined to provide reproducible and efficient methods in growth chamber testing and field experiments for evaluating plant disease resistance. For this, already-known inoculation methods, inoculum densities, and incubation temperature, and plant growth stages at the time of inoculation were compared using tobacco cultivars resistant (Nicotiana tabacum cv, NC95), moderately resistant (N. tabacum cv. SPG70), and susceptible (N. tabacum BY4) to the bacterial disease. It was determined that root-dipping of tobacco seedlings at six true leaf stage into the bacterial suspension with inoculum level of $10^8$ colony-forming units (CFU)/ml for 20 min before transplanting was simple and most efficient in testing for resistance to the bacterial wilt of tobacco caused by R. solanacearum, for which disease incidences and severities were examined at 2 weeks of plant growth after inoculation at $20{\sim}25^{\circ}C$ in a growth chamber. These experimental conditions could discriminate one tobacco cultivar from the others by disease severity better than any other experimental conditions. In field testing, the optimum time for examining the disease occurrence was late June through early July. These results can be applied to establishing a technical manual for the screening of resistant tobacco cultivars against the bacterial wilt caused by R. solanacearum.

• Horticultural Science & Technology
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• 제35권4호
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• pp.402-409
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• 2017

We performed seed germination tests to investigate the effects of seed sowing orientation on germination viability on peanut (Arachis hypogaea L.) sprouts. Specifically, we assessed the influence of seed sowing orientation on germination rate, seedling weight, and seedling length, as well as the seedling vigor index. The seeds were sown in oak tree sawdust at 3.0 cm depth. Four seed orientations were tested: vertical with the hypocotyl end down, vertical with the hypocotyl end up, horizontal with the hypocotyl end down, and horizontal with the hypocotyl end up. The mean seed germination percentages of the four seed orientations were significantly different (p < 0.01) and ranged from 25 to 91.7%. The vertical orientation with hypocotyl-end-down and hypocotyl-end-up orientations showed the highest (91.7%) and lowest (25%) germination rates, respectively. The vertical orientation with the hypocotyl end down produced the heaviest (4.9 g) seedlings and the longest hypocotyls (4.65 cm). This orientation also produced the longest true leaf + epycotyl (2.15 cm) and had the highest seedling vigor index (197.1). The seedlings had a straight growth pattern, whereas seedlings from seeds sown with the hypocotyl up had an awkward plumular hook shape. Taken together, to produce peanut sprouts, we recommend placing the seeds vertically with the hypocotyl end down because this orientation leads to a high germination rate, high biomass production, and high overall seedling quality.

• Horticultural Science & Technology
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• 제32권2호
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• pp.227-234
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• 2014

The responses to chilling temperature of 12 Korean cucumber varieties was compared to those of two U.S.A. (previously determined cold tolerant NC76 and 'Chipper'), and Chinese and Japanese germplasms. Seedlings of each entry were exposed to $4^{\circ}C$ (Experiment 1) and $1^{\circ}C$ (Experiments 2 and 3) at the first-true leaf stage for eight and nine hours, respectively, under 80% relative humidity (RH) and $149{\mu}moles{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux (PPF). The chilling response [damage rating (DR)] of each accession was based on visual ratings (1 to 5) after treatment, where 1 = no damage, 2 = slight, 3 = moderate, 4 = advanced, and 5 = severe damage. Predictably the cumulative average DR of chilling tolerant line NC76 and 'Chipper' after chilling w as 1 and 1.1, respectively. Korean 'Nacdongchungjang' was most sensitive to chilling temperatures [DR = 2.3] when compared to the other entries examined. The sensitivity to chilling of 'Nacdongchungjang' was followed by Chinese 'Dongguan' [DR = 1.7]. In contrast, 'Saeronchungjang' (DR = 1) and 'Janghyungnachap' (DR = 1) were the most chilling tolerant of the Korean accessions examined and equivalent to the response of line NC76 and 'Chipper'. Nevertheless, chloroplast type genotyping of these accessions with known chilling-linked sdCAPS genomic markers revealed genotypic differences between chilling tolerant lines (NC76 and 'Chipper') and all Korean lines examined.

• Journal of Bio-Environment Control
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• 제6권4호
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• pp.310-316
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• 1997

This experiments comparisons were conducted to establish the effective sap analytical method including quantitative analysis of nitrate and phosphorous, sap extraction method, extraction rate, macerating time and sampling position. Earl's Favorite muskmelon was sown on February 11th, 1993 and transplanted to rockwool slab when three to fourth true leaf appeared. Non-circulating system was employed in this experiment. Sap testing using a sample provides an immediate analysis of nutritional status of the crop and potential problems can be recognized and corrective measures taken. Some methods were recommended UV-spectroscopy for the analysis of nitrate nitrogen, Vanad molybdenium acid method for phosphorous. Optimal extraction rate of sample to water was determined as one to four and the best result observed at 60seconds in macerating duration. Further trials would be requested about sampling time due to the difference according to the sampling time. Given sampling position was favorable for the sap analysis.