• 생명과학회지
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• 제5권2호
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• pp.1-1
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• 1995

Casein or soybean protein was subjected to there action with caffeic acidtyrosinase system at 30-35$\circ$C, pH 6.8 with aeration for 5hr. The resulting brown proteins were washed with acetone until the washings were on longer colored. However, modified protein still retained a light brown. The effects of the modified proteins and brown compounds on male Wistar strain rats were studied by pair-feeding of a cholesterol-free diet for 14days. Significant decrease in protein digestibility for the rats fed with the modified proteins were observed. Weight gain and protein digestibility were not influenced by feeding brown compounds, but the feeding of brown compound from casein caused an enlargement of caecum. The concentrations of serum cholesterol and triglyceride in the rats fed with modified proteins and brown compounds were mostly unchanged against the rats fed with untreated proteins. These results suggest that the decrease in protein digestibility induced by enzymic browning-reaction did not cause the decrease in concentration of serum cholesterol.

• Food Science and Biotechnology
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• 제14권2호
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• pp.290-296
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• 2005

Effects of water extract obtained from Chrysanthemum boreale M. (CE) on serum enzyme activities and Kupffer cells of carbon tetrachloride ($CCl_4$)-induced rats were investigated. Thirty-two healthy male Sprague-Dawley rats were divided into normal (N), $CCl_4$-induced (T), CE-supplemented (C), and $CCl_4$-induced and CE-supplemented (TC) groups. $CCl_4$ injection significantly increased aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, and alkaline phosphatase activities in serum. Significant increases in total cholesterol and triglyceride concentrations were also observed in $CCl_4$-induced rats. Oral administration of CE at 300 mg/kg body weight significantly decreased serum enzyme levels and suppressed $CCl_4$ hepatotoxicity-induced lipid profile changes. Histological findings showed fatty change, fibrosis and increased number of Kupffer cells in T group. Electron microscopic examination showed increased lysosome content and dilation of rough endoplasmic reticulum within Kupffer cells in T group, whereas CE supplement attenuated liver injury in $CCl_4$-induced liver. These results indicated CE could significantly alleviate CC4-induced hepatotoxicity injury.

• Asian-Australasian Journal of Animal Sciences
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• 제13권4호
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• pp.516-520
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• 2000

The present study was conducted to evaluate effect of fermented chub mackerel extract (FCME) on lipid metabolism of rats fed a diet supplemented with 1% cholesterol. Four week-old male rats were divided into three groups of 15 rats with 0, 0.1% or 0.2% FCME supplementation. In comparison with control, rats fed 0.2% FCME showed reduction of activities of acetyl-Coenzyme A carboxylase (p<0.05), 3-hydroxy-3-methyl glutaryl Coenzyme A reductase (p<0.01) and fatty acid synthetase (p<0.01). Rats fed 0.2% FCME also showed reductions in free cholesterol in the liver (p<0.05), and in concentrations of free cholesterol (p<0.05), LDL+VLDL-cholesterol (p<0.05), triglyceride (p<0.01) and phospholipid (p<0.01) in the plasma. Plasma HDL-cholesterol concentration was significantly (p<0.05) higher in treatment groups as compared with control group. Atherogenic index was also significantly lower in rats fed 0.1% or fed 0.2% supplement diet, whereas bile acid in feces was not significantly affected. The current study showed that 0.2% inclusion level of the fermented chub mackerel extracts might have hypolipidemic properties.

• 환경생물
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• 제22권1호
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• pp.141-147
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• 2004

In order to investigate whether or not the alcohol-treated rat liver cells can be protected by Korean Citrus junos and medicinal herbs, We compared the serum biochemistry of rats administered both alcohol and the complex of Korean Citrus junos and medicinal herbs to control rats treated with alcohol alone. The activities of Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) in the citron 3 (Citron 3, less mellowed citron which was ripened for three months)＋Phellinus linteus, Alnus japonica, Dendropanax morbifera and citron 4 (Citron 4, completely mellowed citron which was ripened fer four months)＋Phaseolus radiatus, Cordyceps militaris group were significantly low when compared with the negative control group (p<0.05). The levels of triglyceride (TG) in all experimental groups were significantly lower than the negative control group (p<0.05). The concentrations of total cholesterol in the citron 3＋Phellinus linteus, Atnus japonica, Dendropanax morbifera and citron 4＋Phaseolus radiatus, Cordyceps militaris, Phellinus linteus were lower than the negative control group (p<0.05). The activities of alcohol dehydyogenase (ADH) in all experimental groups were significantly high when compared with the normal control group (p<0.05). These results suggest that the complex of Korean Citrus junos and medecinal herbs could be an excellent candidate for protecting vat liver cell damage induced by alcohol.

• Preventive Nutrition and Food Science
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• 제19권3호
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• pp.156-163
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• 2014

We investigated the effects of a high-protein diet and resveratrol supplementation on immune cells changes induced by abdominal irradiation in rats. Female Wistar rats were divided into 5 groups: 1) control diet, 2) control diet with irradiation 3) 30% high-protein diet with irradiation, 4) normal diet with resveratrol supplementation and irradiation, and 5) 30% high-protein diet with resveratrol supplementation and irradiation. We measured blood protein and albumin concentrations, lipid profiles, white blood cell (WBC) counts, proinflammatory cytokine production, and splenocyte proliferation in rats that had been treated with a 17.5 Gy dose of radiation 30 days prior. A high-protein diet affected plasma total cholesterol and very low density lipoprotein-cholesterol levels, which were increased by the radiation treatment. In addition, the lymphocyte percentage and immunoglobulin M (IgM) concentration were increased, and the neutrophil percentage was decreased in rats fed a high-protein diet. Resveratrol supplementation decreased the triglyceride (TG) level, but increased the IgM concentration and splenocyte proliferation. Proinflammatory cytokine production was lower in rats fed a high-protein diet supplemented with resveratrol than in rats fed a control diet. The results of the present study indicate that high-protein diets, with or without resveratrol supplementation, might assist with recovery from radiation-induced inflammation by modulating immune cell percentages and cytokine production.

• Asian-Australasian Journal of Animal Sciences
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• 제13권8호
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• pp.1050-1053
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• 2000

Biological evaluation of residual malathion after 168 hrs of single dipping exposure of White Leghorn hens to different concentrations (0, 0.5, 1.0 and 1.5%) of pesticides was investigated. Thirty-two male albino rats divided into four groups of eight each were kept on 20% isoproteinous diet prepared from the meat of these malathion dipped hens. After 30 days feeding trial, the rats were killed by decapitation. No significant change was found in erythrocytes. However, the triglyceride concentration in brain tissue was increased significantly (p<0.05) when dose level of pesticide was 1% in dipping solution. Similarly, malathion exposed poultry meat failed in altering any significant change in the pancreatic amylase and lipase activities of rats. This study concludes the virtual absence of toxic accumulation of pesticide in the meat of birds after 168 hrs of exposure in usual concentration range upto 1.5%.

• 한국식품영양과학회지
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• 제26권3호
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• pp.507-513
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• 1997

This study was carried out to examine whether kimchi has hypolipidemic effect and to know how it exert lipid-lowering effect in rats. Male Sprague-Dawley rats were fed with kimchi-fee diet, or 3%, or 5%, or 10% kimchi diets for 6 weeks. Plasma cholesterol level was lowered in rats fed all concentrations of kimchi diets, and plasma triglyceride(TG) level was lowered in 10% kimchi diet group compared with that of control significantly(p<0.05). Th intake of kimchi lowered VLDL-cholesterol and VLDL-TG levels, whereas increased HDL-cholesterol level significantly(p<0.05). LDL-cholesterol level was lowered only in 5% kimchi diet group and LDL-TG level was lowered in all kimchi diet groups compared with those of control significantly (p<0.05). the intake of 5% and 10% kimchi diets also lowered the levels of hepatic cholesterol, TG, total lipid, and apolipoprotein B, whereas increased the levels of fecal total fat, cholesterol, TG, and apolipoprotein A-1 significantly(p<0.05). Triiodothyronine(T$_3$) level was elevated in rats fed kimchi diet, whereas thyroxine(T$_4$) level was not affected by kimchi treatment. These observations support that the intake of kimchin in rats loweres plasma and hepatic lipid levels by increasing the excretion of TG and cholesterol through feces, by the elevation of T$_3$ level, and by the altered lipoprotein metabolism.

• Asian-Australasian Journal of Animal Sciences
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• 제18권8호
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• pp.1105-1109
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• 2005

The effect of dietary fatty acid supplementation on milk production, milk composition and plasma constituents in dairy cows was examined. Dietary fatty acids including mainly palmitic acid and stearic acid were given to cows from 2 weeks before and 8 weeks after parturition. Weekly gain of daily milk production was increased gradually during early lactation period and reached a plateau at 4 weeks after parturition. Weekly gain of daily milk production in lactating cows received dietary fatty acids was significantly higher than that of cows given a control diet alone. Although milk lactose concentration was slightly decreased by dietary fatty acid supplementation, milk fat and protein were not significantly influenced by dietary fatty acid supplementation. Dietary fatty acids did not affect plasma concentrations of triglyceride, non-esterified fatty acids, total cholesterol, high-density lipoprotein cholesterol and glucose during postpartum. It is suggested that dietary fatty acid supplementation has the potency to enhance energy balance and improve milk yield without any adverse effects on milk composition.

• Asian-Australasian Journal of Animal Sciences
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• 제23권6호
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• pp.799-805
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• 2010

Since the consumption of spent laying hens as roasted skewered meat increases, the effects of various carotenoids on pigmentation and antioxidant activity were tested with 62-wk-old 250 ISA brown laying hens to improve the quality of chicken meat. In a 6-wk feeding trial, 4 carotenoids with different polarity (${\beta}$-8-apo-carotenoic acid ethyl ester (ACAEE)>astaxanthin>canthaxanthin>${\beta}$-carotene) at 100 mg carotenoid/kg feed were used. The more polar the carotenoids, the higher were the levels in blood. After 5-wk adaptation, the concentrations of astaxanthin, canthaxanthin, and ACAEE in blood were -4 ${\mu}g/ml$. Canthaxanthin decreased significantly (p<0.05) the level of total blood cholesterol. Decreases in blood triglyceride by all carotenoids used were significant. ACAEE and astaxanthin tended to increase skin yellowness of thigh, breast, and wing proportionally to feeding period. In the case of polar carotenoids (ACAEE and astaxanthin), the longer the period of feeding, the higher the accumulation in skin was observed. Only astaxanthin was effective against the production of lipid peroxides in skin. Conclusively, out of the commercially available carotenoids we tested, astaxanthin is recommended for pigmentation of skin and inhibition of lipid oxidation.

• 한국지역사회생활과학회지
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• 제14권3호
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• pp.59-65
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• 2003

This study was carried out to investigate the supplementary effects of the rice germ oil compared with soy bean oil on lipid metabolism of non-insulin dependent diabetic mice. Forty diabetic KK mice were fed two kinds of experimental diets with 20% lipid from soy bean oil as a control(CO) and rice germ oil(RG) for 8 weeks, respectively. Diet intake, body weight, organs weights and lipids levels of serum, liver and feces were measured. There was no significant difference in food and water intake, body weight gain and organs weights between experimental groups. But the concentrations of serum total cholesterol and LDL-cholesterol were lower in RG group than in CO group. The hepatic total lipid and total cholesterol levels of RG group were significantly lower than those of CO group. The contents of total lipid, triglyceride and total cholesterol excreted in feces of RG group were higher than those of CO group. These results suggested that rice germ oil can reduce serum total cholesterol and LDL-cholesterol and hepatic total lipid concentration of non-insulin dependent diabetic mice compared with soybean oil due to increasing fecal lipid excretion. But we need to investigate the hypolipidemic effect of this oil by supplementary level and period.