• Journal of Life Science
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• v.21 no.9
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• pp.1226-1233
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• 2011

In neurons, kinesin is the molecular motor that transport cargos along microtubules. KIF5s (alias kinesin-I), are heterotetrameric motor conveying cargos, but the mechanism as to how they recognize and bind to a specific cargos has not yet been completely elucidated. To identify the interaction proteins for KIF5C, yeast two-hybrid screening was performed, and specific interaction with the $\underline{S}$am68-$\underline{l}$ike $\underline{m}$ammalian protein $\underline{2}$ (SLM-2), a member of the $\underline{s}$ignal $\underline{t}$ransducers and $\underline{a}$ctivators of $\underline{R}$NA (STAR) family of RNA processing proteins, was found. SLM-2 bound to the carboxyl (C)-terminal region of KIF5C and to other KIF5 members. The C-terminal domain of Sam68, SLM-1, SLM-2 was essential for interaction with KIF5C in the yeast two-hybrid assay. In addition, glutathione S-transferase (GST) pull-downs showed that SAM68, SLM-1, and SLM-2 specifically interacted to Kinesin-I complex. An antibody to SAM68 specifically co-immunoprecipitated SAM68 associated with KIF5s and coprecipitated with a specific set of mRNA. These results suggest that Kinesin-I motor protein transports RNA-associated protein complex in cells.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.363-370
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• 2014

Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control.

• Applied Biological Chemistry
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• v.37 no.5
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• pp.361-369
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• 1994

To study the light-induced expression mechanism and protein transport into the chloroplast, a rice genomic clone (GrbcS) for the small subunit of ribulose 1,5-bisphosphate carboxylase (rbcS) was isolated and its nucleotide sequence was determined. Nucleotide sequence analysis of GrbcS revealed that the gene consists of two exons interrupted by an intron, encoding a protein of 175 amino acids including a transit peptide of 47 amino acids. These structural features of GrbcS are consistent with those of other rbcS genes from monocot species. Genomic Southern blot analysis suggested that the rbcS genes are present as a relatively small multigene family in the rice genome. Comparison of the nucleotide and deduced amino acid sequences to other rice rbcSs shows close sequence similaritiy. Conserved DNA sequences present in other light-responsive genes are also found in the 5’ upstream region of GrbcS such as G-box, 3AF1-binding site and GATA site. The possible function of these putative regulatory elements are discussed.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.2
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• pp.177-186
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• 2003

The influences of horizontal and vertical flow components including the stratification of water column and the wind field on the formation of oxygen-deficient water in summer in Jindong Bay, northern part of Chinhae Bay, were examined. Temperature, salinity and dissolved oxygen in seawater, and direction and velocity of wind were observed in Jindong Bay from March 1998 to February 1999. Low concentration of 5 mg/L in dissolved oxygen (DO) appeared at the bottom layer from May to September. Extremely low DO concentration less than 3 mg/L was investigated in summer (July to August) when stratification was strongest due to abrupt vertical gradients of temperature and salinity in water column. Bottom waters with the extremely low DO concentration were observed even in spring (May to June) at the inner part of the bay. In summer (August to September), the bottom waters with the low DO concentration (less than 5 mg/L) existed at the water depth from 4 to 6 m, being moved upward to the surface layer compared to other seasons. Vertical components of residual flow, calculated by the direction and velocity of wind, in Jindong Bay in summer showed that locally prevailed northerly and westerly wind resulted in downwelling flow at the outer part of the bay and conversely, upwelling at the inner part of the bay. In addition, bottom current at the outer part corresponding to the downwelling area directed to the inner part, probably resulting in a transport of the particulate organic matter settled at the bottom waters to the inner part of the bay. The oxygen-deficient watermass, which was formed at the bottom layer of the inner part, was likely to transported to the surface layer by the upwelling flow.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.4
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• pp.514-520
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• 2019

This study investigated the improved lipid metabolism effect of 3T3-L1 cells induced by adipocytes using the dichloromethane (DCM) fraction in the organic solvent extract of Wassong (Orostachys japonicus). To confirm the cell cytotoxicity, each of 6 fractions of organic solvent extracts (EtOH, Hexane, DCM, EtOAc, BuOH, and H₂O) was examined using MTS assay. As a result, it was confirmed that the DCM extract was stable over the whole range of concentrations, and a DCM fraction was used to confirm the improved lipid metabolism effect. Lipid excretion was measured to confirm the change of lipid metabolism. 3T3-L1 cells induced by adipocytes were treated with DCM extract and stained with oil-red O to evaluate lipid accumulation. As a result, it was confirmed that the lipid efflux was significantly improved. In order to confirm the mechanism of lipid efflux, the mRNA expressions of ABCA1 and ABCG1, which are lipid transport proteins, were confirmed by real-time PCR. Therefore, the present study confirmed that the DCM extract from Orostachys japonicus has the effect of improving the lipid metabolism on 3T3-L1 adipocytes. In addition, the results of this study will be used as the basis for the development of functional foods using Orostachys japonicus and also for conducting research on the detailed mechanisms.

• Journal of KIISE:Information Networking
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• v.27 no.3
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• pp.348-359
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• 2000

Most of the important real time multimedia applications require multipoint transmissions. To support these applications in ATM based Intermet environments, it is important to provide efficient IP multicast transportations over ATM networks. IETF proposed MARS(Multicast Address Resolution Server) as the service architecture to transport connectionless IP multicast flows over connection oriented ATM VCs. MARS assumes UNI3.0/3.1 signalling. Since UNI3.0/3.1 does not provide any means for receivers to request a join for a multicast ATM VC, MARS provides overlay service to relay join request from IP multicast group members to the sources of the multicast group. Later on, ATM Forum standardized UNI4.0 signalling which is provisioned with a new signalling mechanism called LIJ(Leaf Initiated Join). LIJ enables receivers to directly signal the source of an ATM VC to join. In this paper, we propose a new service architecture providing IP multicast flow transportation over ATM networks deploying UNI4.0 signalling. The proposed architecture is named UNI4MARS. It comprises service components same as those of the MARS. The main functionality provided by the UNI4MARS is to provide source information to the receivers so that the receivers may exploit LIJ to join multicast ATM VCs dynamically. The implementation overhead of UNI4MARS and that of MARS are compared by a course of simulations. The simulation results show that the UNI4MARS supports the dynamic IP multicast group changes more efficiently with respect to processing, memory and bandwidth overhead.

• Journal of Life Science
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• v.31 no.10
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• pp.944-953
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• 2021

Lignin is a complex phenylpropanoid polymer abundant in the cell walls of vascular plants. It is mainly presented in conducting and supporting tissues, assisting in water transport and mechanical strength. Lignification is also utilized as a defense mechanism against pathogen infection or wounding to protect plant tissues. The monolignol precursors of lignin are synthesized by cinnamyl alcohol dehydrogenase (CAD). CAD catalyzes cinnamaldehydes to cinnamyl alcohols, such as p-coumaryl, coniferyl, and sinapyl alcohols. CAD exists as a multigenic family in angiosperms, and CAD isoforms with different functions have been identified in different plant species. Multiple isoforms of CAD genes are differentially expressed during development and upon environmental cues. CAD enzymes having different functions have been found so far, showing that one of its isoforms may be involved in developmental lignification, whereas others may affect the composition of defensive lignins and other wall-bound phenolics. Substrate specificity appears differently depending on the CAD isoform, which contributes to revealing the biochemical properties of CAD proteins that regulate lignin synthesis. In this review, details regarding the expression and regulation of the CAD family in lignin biosynthesis are discussed. The isoforms of the CAD multigenic family have complex genetic regulation, and the signaling pathway and stress responses of plant development are closely linked. The synthesis of monolignol by CAD genes is likely to be regulated by development and environmental cues as well.

• Journal of agriculture & life science
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• v.51 no.1
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• pp.187-192
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• 2017

In various crops, seedlings are preferred to seeds for faster and more effective growth, so transplanters are used for them. This 2-row transplanter was developed to promote the mechanization of vegetable transplanting work which depends on human power. and it can automatically supply seedling tray and transport picked up seedling to the planting hopper. Also, we judged performance of transplanter with comparing seedling missing plant ration according to two types of pick-up method. Result of experiment, in finger-type picking up of 265 seedlings, missing plant ratio was 13.7% with 17 failures of pick-up and 15 collapse of bed soil. and In fork-type picking up of 200 seedlings, failure of pick-up was not appeared and missing plant ratio was low as 4% with 6 dropped during transfer. Therefore for 2-row automatic transplanter, fork-type pick-up device was found to be compatible.

• Journal of Wetlands Research
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• v.23 no.2
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• pp.154-162
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• 2021

The naturally opened Han river estuary is a place where the flows of the Han river, Imjin river, Yaesung river meet with West Sea of Korea, so the hydrodynamic mechanism(Impact-Response) structure of Han river estuary is complex. Continuous observation and measurement due to the morphological characteristics at the estuary are required to maintain the estuary environment and river management facilities. However, the Sannam wetland(the study area) is in the military operation area. Therefore, Sannam wetland has the limited access under the control from military office. In 2020, there had a natural disaster due to flooding in August and COVID-19, and it made a survey hard. The noncontact survey technique, the analysis of LANDSAT images at Sannam wetland, was applied to analyze riverbed fluctuation and morphological transformation around Sannam wetland. LANDSAT images obtained from EarthExplorer, USGS and analyzed by QGIS. The analysis was performed based on the area and the distance near Sannam wetland. As a result, an erosion was happened on the downstream of the study area, and the upstream of the study area did not have any serious sediment transport. Considering the resolution of LANDSAT images, this noncontect survey technique is applicable to manage the study area. From the analysis of LANDSAT images, it is assumed that the tidal effect is greater than the inflow from the upstream. The pattern change of tidal response causes the damage of the river facilities near the Hangang river estuary.

• Journal of the Korea institute for structural maintenance and inspection
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• v.24 no.6
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• pp.10-16
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• 2020

Deterioration of reinforced concrete structure caused by chloride ingress is the main issue and regrading this, many studies have been investigated with both experiments and computational modelling. In addition to chloride diffusion, chloride sorption should be considered as a chloride transport mechanism in concrete structure and cracks formed in concrete structures are the main variable to evaluate the performance of the structures. In this study, after making two types of cracks width (0.1 and 0.3 mm) in reinforced concretes, chloride absorption tests were performed. Weight change and colour change using 0.1 AgNO3 solution from the samples were performed to measure chloride ingress. Image processing was also carried out to quantify range of colour change in carck face. From the result, it were confirmed that the amount of chloride absorption increases with exposure time and increasing crack width, and chlorides reached at steel depth within 1 hour. It would be possible that chloride can move through interface bewteen steel and concrete, thereby further study regarding this is required.